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De?ning gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

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Journal of Genetics and Genomics 2019年第1期46卷 19-30页

作者： Yuan Gao Ying mao Rong-Gang Xu Ruibao Zhu Ming Zhang Jin Sun Da Shen Ping Peng Ting Xie Jian-Quan Ni PKU-THU Joint Center for Life Sciences College of Life SciencesSchool of MedicineTsinghua UniversityBeijing100084China Gene Regulatory Lab School of MedicineTsinghua UniversityBeijing100084China Stowers Institute for Medical Research 1000 East 50th StreetKansas CityMO64110USA Tsingdao Advanced Research Institute Tongji UniversityQingdao266000China

In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study,we identified 173 EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.

关键词： Germline stem cells Escort cells In vivo screen

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Differential expression, molecular cloning,and characterization of porcine beta defensin 114

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Journal of Animal Science and Biotechnology 2019年第4期10卷 884-894页

作者： Guoqi Su Kunhong Xie Daiwen Chen Bing Yu Zhiqing Huang Yuheng Luo Xiangbing mao Ping Zheng Jie Yu Junqiu Luo Jun He Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130People’s Republic of China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengdu 625014People’s Republic of China

Background:β-defensins have attracted considerable research interest because of their roles in protecting hosts from various *** study was conducted to investigate the expression profiles of the porcineβ-defensin114(PBD114)in different breeds and in response to ***,the function of PBD114 protein was partially ***:Six Tibetan pigs(TP)and six DLY(Duroc×Landrace×Yorkshire)pigs were slaughtered to explore the expression profiles of PBD114 in different breeds and *** infection models,sixteen DLY pigs were divided into two groups and challenged either with sterile saline or *** *** recombinant protein PBD114(r PBD114)was obtained by using a heterologous expression system in ***:PBD114 gene was highly expressed in tissues such as the intestine,liver,spleen,and ***,the expression level of PBD114 gene was higher in the TP pigs than in the DLY pigs(P<0.05),and was significantly elevated upon *** K88 challenge(P<0.05).The nucleotide sequences of PBD114 from Tibetan and DLY pigs was identical,and both showed a 210-bp open reading frame encoding a 69-amino acid mature *** explaore the function of PBD114 protein,PBD114 gene was successfully expressed in *** Origami B(DE3)and the molecular weight of the r PBD114 was estimated by SDS-PAGE to be 25 k *** r PBD114 was purified and mass spectrometry verified the protein as ***,r PBD114 showed antimicrobial activities against *** DH5αand *** K88,and the minimal inhibitory concentrations(MICs)were 64 and 128μg/m L,*** and cytotoxicity assays showed that r PBD114 did not affect cell viability under physiological ***:PBD114 is an infection response gene that is differentially-expressed between different porcine breeds and *** antimicrobial activity of PBD114 protein,against pathogens such as the *** K88,suggested that it may serve as a candidate for the substitutio

关键词： Antimicrobial activity DLY E.coli Porcine beta defensin 114 Tibetan

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Effect of Long-Term Thermal Exposures on Tensile Behaviors of K416B Nickel-Based Superalloy

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Acta Metallurgica Sinica(English Letters) 2020年第12期33卷 1699-1708页

作者： mao-Kai Chen Jun Xie De-Long Shu Gui-Chen Hou Shu-Ling Xun Jin-Jiang Yu Li-Rong Liu Xiao-Feng Sun Yi-Zhou Zhou Institute of Metal Research Chinese Academy of SciencesShenyang 110016China Shenyang University of Technology Shenyang 110870China

The effect of long-term thermal exposure on the tensile behavior of a high W content nickel-based superalloy K416B was *** microstructure and the deformation characteristics were observed by scanning electron microscopy and transmission electron microscopy,and the phase transformation of the alloy during long-term thermal exposure was analyzed by X-ray diffraction patterns and differential thermal *** showed that after thermal exposure at 1000℃,the MC carbides in the K416 B alloy decomposed into M_(6)*** tensile deformation,dislocations slipping inγmatrix crossed over the M_(6)C by Orowan bowing *** the increase of thermal exposure time,the secondary M_(6)C reduced greatly the yield strength of the alloy at room ***,the continuous distribution of the secondary M_(6)C with great brittleness in the grain boundary could become the main source of crack,which might change the fracture characteristic of the alloy from trans-granular to intergranular.

关键词： K416B superalloy Thermal exposure Deformation mechanism Fracture characteristics

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Adomian修正分解法的后处理方法比较

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计算力学学报 2017年第4期34卷 517-522页

作者：毛崎波扬州大学机械工程学院扬州225127 南昌航空大学飞行器工程学院南昌330063

Adomian修正分解法在求解非线性微分方程中得到广泛应用。Adomian修正分解法的主要特点在于计算简单快速,并且不需要进行线性化或离散化。但是Adomian修正分解法的计算精度取决于其收敛域。为了扩大Adomian修正分解法的收敛域,需要对所... 详细信息

Adomian修正分解法在求解非线性微分方程中得到广泛应用。Adomian修正分解法的主要特点在于计算简单快速,并且不需要进行线性化或离散化。但是Adomian修正分解法的计算精度取决于其收敛域。为了扩大Adomian修正分解法的收敛域,需要对所得解进行后处理,目前常见的后处理方法包括Padé近似、LaplacePadé近似和多步迭代方法。本文首先简要回顾了Adomian修正分解法,然后讨论了这三种后处理方法,最后通过Duffing振子为例对这些后处理方法的优缺点进行讨论和分析。数值计算结果表明,多步迭代方法能够加速Adomian修正分解法解的收敛,并扩大其收敛域。

关键词： Adomian修正分解法后处理方法 Padé近似 Laplace-Padé近似多步迭代方法

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Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression

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Journal of Molecular Cell Biology 2022年第1期14卷 32-46页

作者： Yuwei Li Chencheng Yang Huishan Wang Ling Zhao Qinghua Kong Yu Cang Shuhua Zhao Longbao Lv Yan Li Bingyu mao Pengcheng Ma State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650203China Experimental Animal Center Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming Institute of Botany Chinese Academy of SciencesKunming 650223China Department of Urology the Affiliated Hospital of Yunnan UniversityKunming 650021China First Affiliated Hospital of Kunming Medical University Kunming 650032China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

Sonic hedgehog (Shh) signaling is essential for the proliferation of cerebellar granule neuron progenitors (CGNPs), and its misregulation is linked to various disorders, including cerebellar cancer medulloblastoma (MB). During vertebrate neural development, RNF220, a ubiquitin E3 ligase, is involved in spinal cord patterning by modulating the subcellular location of glioma-associated oncogene homologs (Glis) through ubiquitination. RNF220 is also required for full activation of Shh signaling during cerebellum development in an epigenetic manner through targeting embryonic ectoderm development. ZC4H2 was reported to be involved in spinal cord patterning by acting as an RNF220 stabilizer. Here, we provided evidence to show that ZC4H2 is also required for full activation of Shh signaling in CGNP and MB progression by stabilizing RNF220. In addition, we found that the ubiquitin E3 ligase RING finger LIM domain-binding protein (RLIM) is responsible for ZC4H2 stabilization via direct ubiquitination, through which RNF220 is also thus stabilized. RLIM is a direct target of Shh signaling and is also required for full activation of Shh signaling in CGNP and MB cell proliferation. We further provided clinical evidence to show that the RLIM‒ZC4H2‒RNF220 cascade is involved in Shh-group MB progression. Disease-causative human RLIM and ZC4H2 mutations affect their interaction and regulation. Therefore, our study sheds light on the regulation of Shh signaling during cerebellar development and MB progression and provides insights into neural disorders caused by RLIM or ZC4H2 mutations.

关键词： ZC4H2 RLIM RNF220 Shh signaling cerebellum medulloblastoma(MB)

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Influence of nitrogen wet deposition on nitrogen output in a typical watershed in the Three Gorges Reservoir Area

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Journal of Mountain Science 2022年第8期19卷 2214-2225页

作者： LU Chen-dong ZHANG Liu-yi YAN Kun WANG Yu-kuan XIONG Shan-gao HUANG Chen-tao LI Ting-zhen NI mao-fei Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir Area Chongqing Three Gorges UniversityWanzhou 404000China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China Institute of Strategic Planning Chinese Academy of Environmental PlanningBeijing 100043China College of Eco-Environmental Engineering Guizhou Minzu UniversityGuiyang 550025China

In order to explore the influence of wet nitrogen(N)deposition on N output in watersheds,this study selected a typical small watershed(Chenjiagou,CJG)in the Three Gorges Reservoir(TGR)area based on one-year observation of rainfallrunoff N *** and sources of N output were clarified by chemical statistics and isotopic abundance,and the contribution of N deposition to the N output was quantified by the output coefficient *** N flux of wet deposition was estimated at 18.53 kg N ha^(-1) yr^(-1),and originated mostly from agricultural *** N was significantly accumulated from upper to lower reaches due to frequent human *** changes of riverine N were strongly affected by chemical fertilizer,with the highest concentration in spring and the lowest in *** was mainly derived from chemical fertilizer,accounting for 38.83% of all sources.N concentration in processes of different rainfall events had a hysteresis effect corresponding to the flow *** rainfall events greatly changed DTN(Dissolved total N)outputs:rainstorm caused 91.26 kg DTN output,which was 11 times that of moderate rain(8.46 kg)and 4 times that of heavy rain(20.80 kg).N deposition contributed 19.89% of riverine N output in the *** results can provide theoretical support for the control of N pollution in the TGR area.

关键词： N wet deposition Riverine N load Isotope Watershed N output

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Assessing genome-wide adaptations associated with range expansion in the pink rice borer, Sesamia inferens

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Insect Science 2024年第5期31卷 1617-1630页

作者： Hongran Li Yan Peng Chao Wu Zhimin Li Luming Zou Kaikai mao Junfen Ping Ryan Buck Scott Monahan Arun Sethuraman Yutao Xiao Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong ProvinceChina Department of Biology San Diego State UniversityCAUSA Department of Ecology&Evolutionary Biology University of CaliforniaLos AngelesCAUSA

Understanding the genetic basis of adaptive evolution following habitat expan-sion can have important implications for pest *** pink rice borer(PRB),Sesamia inferens(Walker),is a destructive pest of rice that was historically restricted to regions south of 34o N latitude in ***,with changes in global climate and farming practices,the distribution of this moth has progressively expanded,en-compassing most regions in North ***,3 highly differentiated subpopulations were discovered using high-quality single-nucleotide polymorphism and structural vari-ant datasets across China,corresponding to northern,southern China regions,and the Yunnan-Guizhou Plateau,with significant patterns of isolation by geographic and envi-ronmental *** estimates of evolutionary history indicate asymmetric migration with varying population sizes across the 3 *** sweep analyses esti-mated strong selection at insect cuticle glycine-rich cuticular protein genes which are as-sociated with enhanced desiccation adaptability in the northern group,and at the histone-lysine-N-methyltransferase gene associated with range expansion and local adaptation in the Shandong *** findings have significant implications for the development of effective strategies to control this pest.

关键词： desiccation adaptability evolutionary history genetic adaptation phylo-geography Sesamia inferens

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地方政府财政效率促进经济增长实证研究——基于监督力度视角

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中国软科学 2019年第6期 99-106页

作者：毛军海南师范大学数学与统计学院

文章基于我国地方政府财政投入产出数据,采用三阶段DEA效率模型测算出地方政府财政效率。研究结果显示:我国地方政府财政效率区域分布上,中部地区财政效率均值较高,西部地区财政效率均值高于东部地区;在剔除环境因素和随机误差影响后,... 详细信息

文章基于我国地方政府财政投入产出数据,采用三阶段DEA效率模型测算出地方政府财政效率。研究结果显示:我国地方政府财政效率区域分布上,中部地区财政效率均值较高,西部地区财政效率均值高于东部地区;在剔除环境因素和随机误差影响后,我国地方政府财政效率有所提升。运用工具变量的动态面板门槛模型估计研究结果表明:当预决算差异、财政违规资金和行政腐败程度处于中高阈值时,地方政府财政效率对经济增长具有显著的负向影响。由此说明,地方政府财政效率对经济增长的影响效应因监督力度不同而有所差异。

关键词：监督力度地方政府财政效率经济增长三阶段DEA模型动态面板门槛模型

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Quantifying the contribution of activity patterns to PM_(2.5)exposure inequity between urban and rural residents by a novel method

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Building Simulation 2024年第8期17卷 1323-1333页

作者： Wei Du Zhanpeng Cui Jinze Wang Yuqiong Wang Yungui Li Xiaoan Li Yan Zhou Tao Jiang Kang mao Xianbiao Lin Jianwu Shi Dengzhou Gao Yiming Qin Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control Faculty of Environmental Science&EngineeringKunming University of Science&TechnologyKunming 650500China Laboratory of Earth Surface Processes College of Urban and Environmental SciencesPeking UniversityBeijing 100871China Department of Environmental Engineering Southwest University of Science and TechnologyMianyang 621010China NHC Key Laboratory of Nuclear Technology Medical Transformation Mianyang Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaMianyang 621000China State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao266100China Key Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education School of Geographical SciencesFujian NormalUniversityFuzhou350117China School of Energy and Environment City University of Hong KongHong KongChina

PM_(2.5)pollution variations in different microenvironments would result in PM_(2.5)exposure inequity between rural and urban *** this study,the real-time PM_(2.5)exposure of urban and rural residents in China was examined based on portable PM_(2.5)sensors together with activity patterns derived from questionnaire surveys,with a focus on students and senior citizens who are sensitive to air *** results showed that PM_(2.5)exposure varied significantly among different resident groups,with higher PM_(2.5)exposure of rural residents than those of urban ***_(2.5)exposure peaks mostly occurred during(Accompanied)cooking activities owing to strong *** and resting were the main activities that affected PM_(2.5)exposures of different resident groups,accounting for 60.7%-94.5%of total daily ***,the long duration of sleeping makes it the predominant activity contributing to PM 25 exposure *** is necessary to obtain point-to-point respiratory volume(respiratory rate)data when measuring real-time PM_(2.5)exposure data and incorporate respiratory volume(respiratory rate)into the analysis of PM_(2.5)*** the first time,this study quantified the PM_(2.5)exposure inequality based on a novel method and can provide useful information for further studies on the exposure inequity.

关键词： PM_(2.5)exposure environmental inequity activity pattern urban and rural difference

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Effect of lump ore ratio on the metallurgical properties of blast furnace charge

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Baosteel Technical Research 2023年第4期17卷 28-34页

作者： TAN Chenglin mao Xiaoming XU Wanren Research Institute Baoshan Iron&Steel Co.Ltd.Shanghai 201999China

We conducted a series of experimental studies on the metallurgical properties of N-lump and F-lump ores used in Baosteel’s blast furnace, including thermal cracking, low-temperature reduction pulverization, reducibility, and droplet *** results show that the thermal burst properties of N-lump ore are better than those of F-lump *** low-temperature reduction degradation index(RDI) pulverization of the charge is the best when the ratios of N-lump ore and F-lump ore account for 35% *** reduction performance of the charge is improved when F-lump and N-lump ores are mixed with *** the softening-melting performance experiment, when the proportion of N-lump ore is 40%,the characteristic area value(S) as the charge permeability index is the *** F-lump ore is mixed with sinter, its droplet performance improves compared with that of single F-lump *** proportion of F-lump ore should not exceed 15%.

关键词： lump ore ratio metallurgical property experiment softening-melting property the RDI pulverization reduction property

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