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Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

Journal of Integrative Plant Biology 2023年第1期65卷 150-166页

作者： Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu jianfei kuang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China

The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ripening.However,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely unknown.Here,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening stage.Consistent with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the ripening.DNA affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB AM1.Stably overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ripening.Moreover,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation activity.Importantly,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by MaMsrA4.Collectively,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9.

关键词： banana EIN3/EIL redox regulation ROS starch degradation

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Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening

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Horticulture Research 2023年第1期10卷 220-231页

作者： Yingying Yang Chaojie Wu Wei Shan Wei Wei Yating Zhao jianfei kuang Jianye Chen Yueming Jiang Wangjin Lu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China

Mitogen-activated protein kinase(MAPK/MPK)cascades play crucial parts in plant growth,development processes,immune ability,and stress responses;however,the regulatory mechanism by which MAPK affects fruit ripening remains largely unexplored.Here,we reported that MaMPK14 cooperated with MaMYB4 to mediate postharvest banana fruit ripening.Transient overexpression of individual MaMPK14 and MaMYB4 in banana fruit delayed fruit ripening,confirming the negative roles in the ripening.The ripening negative regulator MaMYB4 could repress the transcription of genes associated with ethylene biosynthesis and fruit softening,such as MaACS1,MaXTH5,MaPG3,and MaEXPA15.Furthermore,MaMPK14 phosphorylated MaMYB4 at Ser160 via a direct interaction.Mutation at Ser160 of MaMYB4 reduced its interaction with MaMPK14 but did not affect its subcellular localization.Importantly,phosphorylation of MaMYB4 by MaMPK14 enhanced the MaMYB4-mediated transcriptional inhibition,binding strength,protein stability,and the repression of fruit ripening.Taken together,our results delineated the regulation pathway of MAPK module during banana fruit ripening,which involved the phosphorylation modification of MaMYB4 mediated by MaMPK14.

关键词： interaction. fir banana

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The membrane lipid metabolism in horticultural products suffering chilling injury

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Food Quality and Safety 2020年第1期4卷 9-14页

作者： Shu-min Liang Jian-fei kuang Shu-juan Ji Qin-fang Chen Wei Deng Ting Min Wei Shan Jian-ye Chen Wang-jin Lu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Post-harvest Science of Fruits and Vegetables College of HorticultureSouth China Agricultural UniversityGuangzhou College of Food Shenyang Agricultural UniversityShenyang City State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou School of Life Science Chongqing UniversityChongqing College of Food Science&Engineering Wuhan Polytechnic UniversityChina

Horticultural commodities suffer chilling injury following exposure to extremely low temperatures,which results in visible symptoms and considerable quality loss.Therefore,it is of significance to understand the mechanism of this physiological disorder and to develop effective strategies to control it.Chilling stress causes alteration in structure and function of the plasma membrane,which is assumed to be the primary event in response to cold stress.During this process,the membrane lipid metabolism plays a pivotal role in membrane fluidity and stability.In this review,we summarized the possible roles of membrane lipid metabolism in the development of chilling injury,having the potential for developing effective strategies to alleviate chilling injury in horticultural products under refrigerated storage in practice.

关键词： cell membrane chilling injury fruits and vegetables lipid metabolism

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A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

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Horticultural Plant Journal 2017年第5期3卷 190-198页

作者： WEI Wei FAN Zhongqi CHEN Jianye kuang jianfei LU Wangjin SHAN Wei Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China Ministry of Agriculture/Engineering Research Center of Southern Horticultural Products Preservation Ministry of Education College of Horticulture South China Agricultural University

Plant homeobox domain(PHD)-type transcription factors(TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay(EMSA) and transient expression analysis demonstrated that Ma PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening.

关键词： banana fruit ripening PHD-type transcription factor cell wall degradation transcriptional regulation

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Molecular Characterization of a Leaf Senescence-Related Transcription Factor BrWRKY75 of Chinese Flowering Cabbage

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Horticultural Plant Journal 2016年第5期2卷 272-278页

作者： TAN Xiaoli FAN Zhongqi LI Lulu WU Ya kuang jianfei LU Wangjin CHEN Jianye State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Engineering Research Center of Southern Horticultural Products Preservation Ministry of Education Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables College of Horticulture South China Agricultural University

WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence.

关键词： Chinese flowering cabbage leaf senescence WRKY transcription factor transcriptional regulation

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The association of HpDof1.7 and HpDof5.4 with soluble sugar accumulation in pitaya fruit by transcriptionally activating sugar metabolic genes

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Food Quality and Safety 2022年第4期6卷 619-628页

作者：牟真靓曾闰曦陈南慧刘宗莉曾泽湘秦永华单伟邝健飞陆旺金陈建业赵亚婷 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of HorticultureSouth China Agricultural UniversityGuangzhouChina

Soluble sugar is one of the most important factors affecting fruit flavor and quality.Here,we report the identification of two Dof(DNA-binding with one finger)transcription factors termed HpDof1.7 and HpDof5.4 and their roles in influencing sugar accumulation in pitayas.HpDof1.7 and HpDof5.4 share a similar expression pattern with sugar metabolism-related genes HpSuSy1 and HpINV2,and sugar transporter genes HpTMT2 and HpSWEET14 during pitayas maturation,and their expression pattern was also consistent with the accumulation of glucose and fructose,which were the predominant sugars in pitayas.HpDof1.7 and HpDof5.4 were both typical nucleus-localized proteins with trans-activation ability.Gel mobility shift assay revealed that HpDof1.7 and HpDof5.4 were bound to promoters of HpSuSy1,HpINV2,HpTMT2 and HpSWEET14.Finally,transient expression assays in tobacco leaves showed that HpDof1.7 and HpDof5.4 increased the activities of HpSuSy1,HpINV2,HpTMT2 and HpSWEET14 promoters,thus facilitating sugar accumulation by transcriptionally enhancing sugar metabolic pathway genes.Our findings provide a new perspective on the regulatory mechanisms of Dof transcription factors in sugar accumulation and pitaya fruit quality formation.

关键词： Pitaya sugar accumulation Dof transcription factors transcriptional activation

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Comparative Study on Deformation Prediction Models of Wuqiangxi Concrete Gravity Dam Based on Monitoring Data

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Computer Modeling in Engineering & Sciences 2022年第4期131卷 49-72页

作者： Songlin Yang Xingjin Han Chufeng kuang Weihua Fang jianfei Zhang Tiantang Yu Hunan Wuling Power Technology Corporation Ltd. Changsha410004China Wuling Power Corporation Ltd. Changsha410004China Nanjing Research Institute of Hydrology and Water Conservation Automation Ministry of Water ResourcesNanjing210012China Department of Engineering Mechanics Hohai UniversityNanjing211100China

The deformation prediction models of Wuqiangxi concrete gravity dam are developed,including two statistical models and a deep learning model.In the statistical models,the reliable monitoring data are firstly determined with Lahitte criterion;then,the stepwise regression and partial least squares regression models for deformation prediction of concrete gravity dam are constructed in terms of the reliable monitoring data,and the factors of water pressure,temperature and time effect are considered in the models;finally,according to the monitoring data from 2006 to 2020 of five typical measuring points including J23(on dam section 24^(#)),J33(on dam section 4^(#)),J35(on dam section 8^(#)),J37(on dam section 12^(#)),and J39(on dam section 15^(#))located on the crest of Wuqiangxi concrete gravity dam,the settlement curves of the measuring points are obtained with the stepwise regression and partial least squares regression models.A deep learning model is developed based on long short-term memory(LSTM)recurrent neural network.In the LSTM model,two LSTMlayers are used,the rectified linear unit function is adopted as the activation function,the input sequence length is 20,and the random search is adopted.The monitoring data for the five typical measuring points from 2006 to 2017 are selected as the training set,and the monitoring data from 2018 to 2020 are taken as the test set.From the results of case study,we can find that(1)the good fitting results can be obtained with the two statistical models;(2)the partial least squares regression algorithm can solve the model with high correlation factors and reasonably explain the factors;(3)the prediction accuracy of the LSTM model increases with increasing the amount of training data.In the deformation prediction of concrete gravity dam,the LSTM model is suggested when there are sufficient training data,while the partial least squares regression method is suggested when the training data are insufficient.

关键词： Wuqiangxi concrete gravity dam deformation prediction stepwise regression model partial least squares regression model LSTM model

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香蕉MaTIFY1转录因子特性及其在成熟过程中基因表达分析

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园艺学报 2017年第1期44卷 43-52页

作者：罗冬兰巴良杰陈建业陆旺金单伟邝健飞华南农业大学园艺学院亚热带农业生物资源保护与利用国家重点实验室南方园艺产品保鲜教育部工程研究中心广东省果蔬保鲜重点实验室广州510642

从香蕉果实中分离获得1个TIFY亚族的转录因子基因,命名为MaTIFY1。该基因含有一个681 bp的ORF,编码一条长度为227 aa,分子量为24.97 kD,等电点为7.85的氨基酸多肽链。MaTIFY1定位于细胞核,并且具有转录激活活性。荧光定量PCR分析表明,Ma... 详细信息

从香蕉果实中分离获得1个TIFY亚族的转录因子基因,命名为MaTIFY1。该基因含有一个681 bp的ORF,编码一条长度为227 aa,分子量为24.97 kD,等电点为7.85的氨基酸多肽链。MaTIFY1定位于细胞核,并且具有转录激活活性。荧光定量PCR分析表明,MaTIFY1随着香蕉果实成熟进程表达明显增强,并且外源丙烯处理可诱导其表达。此外,MaTIFY1启动子活性受乙烯利诱导激活,进一步表明MaTIFY1可能参与香蕉果实成熟的调控。原核表达并纯化了MaTIFY1重组蛋白。

关键词：香蕉 TIFY 转录因子果实成熟基因表达

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香蕉MaWRKY31转录因子的特性及对MaACS1、MaACO1和MaSAG1的调控

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食品科学 2017年第6期38卷 13-19页

作者：范中奇卓远芳符亚莉谭小丽邝健飞陆旺金陈建业华南农业大学园艺学院亚热带农业生物资源保护与利用国家重点实验室南方园艺产品保鲜教育部工程研究中心广东省果蔬保鲜重点实验室广东广州510642

从香蕉果实中克隆得到1个WRKY转录因子,命名为Ma WRKY31。氨基酸序列比对及进化树分析发现Ma WRKY31具有一个典型的WRKY结构域,与拟南芥WRKY31和番茄Sl WRKY6同源性较高,且同属于第Ⅱ类b亚族。实时荧光定量聚合酶链式反应表明外源乙烯... 详细信息

从香蕉果实中克隆得到1个WRKY转录因子,命名为Ma WRKY31。氨基酸序列比对及进化树分析发现Ma WRKY31具有一个典型的WRKY结构域,与拟南芥WRKY31和番茄Sl WRKY6同源性较高,且同属于第Ⅱ类b亚族。实时荧光定量聚合酶链式反应表明外源乙烯处理诱导了Ma WRKY31的表达,并且随着香蕉果实的成熟衰老,其表达明显增强。亚细胞定位和转录活性分析显示Ma WRKY31定位于细胞核,是一种核蛋白,并且具有转录激活活性。双荧光素酶瞬时表达分析显示,Ma WRKY31可以激活乙烯合成基因Ma ACS1和Ma ACO1,以及成熟衰老相关基因Ma SAG1的启动子活性。这些结果表明Ma WRKY31可能通过调控乙烯合成和成熟衰老相关基因的表达来参与香蕉果实成熟衰老过程,加深了对香蕉果实成熟衰老转录调控机制的认识。

关键词：香蕉果实成熟 WRKY转录因子转录调控

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黄瓜果实耐冷性与CsHSFs基因表达关系的研究

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华南农业大学学报 2015年第5期36卷 85-91页

作者：陈珊王晓晨邝健飞陈建业陆旺金华南农业大学园艺学院广东广州510642

【目的】研究CsHSFs基因的表达特征与热处理和冷锻炼处理提高黄瓜果实耐冷性的关系.【方法】测定了低温(8℃)贮藏过程中热处理(低温贮藏前50℃热水处理1 min)、冷锻炼处理(15℃预贮2 d后再低温贮藏)和对照处理的黄瓜果实的相对电导率、... 详细信息

【目的】研究CsHSFs基因的表达特征与热处理和冷锻炼处理提高黄瓜果实耐冷性的关系.【方法】测定了低温(8℃)贮藏过程中热处理(低温贮藏前50℃热水处理1 min)、冷锻炼处理(15℃预贮2 d后再低温贮藏)和对照处理的黄瓜果实的相对电导率、可溶性固形物(TSS)含量、叶绿素含量和果实色泽等生理指标,并用qRT-PCR法检测CsHSFs基因的表达特征.【结果和结论】热处理和冷锻炼处理均可提高果实耐冷性,主要表现为热处理和冷锻炼处理均显著抑制了果实相对电导率的升高和叶绿素含量的降低,较好地保持了果实的色泽和商品性状,相比较而言,冷锻炼处理减轻果实冷害的效果比热处理明显,同时,热处理与冷锻炼处理都不影响果实TSS含量.并且,CsHSF7和CsHSF11基因表达水平与热处理和冷锻炼诱导的果实耐冷性密切相关,但其参与冷害的机制可能不同,体现为CsHSF7在酵母中具有转录激活活性,而CsHSF11在酵母中不具有转录激活活性.

关键词：黄瓜果实热处理冷锻炼处理冷害 CsHSFs基因

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