检索结果-南通市图书馆

A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed

引用

Journal of Integrative Agriculture 2024年第5期23卷 1481-1493页

作者： Qianwei zhang Yuanyi Mao Zikun Zhao Xin Hu Ran Hu Nengwen Yin Xue Sun Fujun Sun Si Chen Yuxiang jiang liezhao liu Kun Lu jiana li Yu Pan Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Southwest UniversityChongqing 400715China Academy of Agricultural Sciences Southwest UniversityChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China

Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and *** this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic *** BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-liKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and *** with this,BnGLK1a-RNAi lines of *** display hypersensitivity to the abscisic acid(ABA)***,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild *** propose that BnGLK1a could be a potential target in breeding for improving rapeseed *** results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.

关键词： Brassica napus BnGLK1a chloroplast development photosynthetic efficiency yield

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

甘蓝型黄籽油菜粒色基因候选区间的QTL-Seq定位及连锁标记开发

引用

西南大学学报（自然科学版） 2024年第4期46卷 2-12页

作者：王瑞郭青青徐新福卢坤李加纳曲存民西南大学西部(重庆)科学城种质创制大科学中心重庆400715

甘蓝型油菜黄籽性状遗传来源较为复杂且易受环境条件影响,基因定位和分子标记开发难度大.相比于传统QTL定位,结合二代深度测序等生信方法直接对甘蓝型油菜黄籽基因进行染色体定位、基因挖掘和标记开发,将有助于黄籽遗传机理的研究.利用... 详细信息

甘蓝型油菜黄籽性状遗传来源较为复杂且易受环境条件影响,基因定位和分子标记开发难度大.相比于传统QTL定位,结合二代深度测序等生信方法直接对甘蓝型油菜黄籽基因进行染色体定位、基因挖掘和标记开发,将有助于黄籽遗传机理的研究.利用甘蓝型油菜黄籽临保系63-2和黑籽67-1配制杂交组合,构建F 2粒色分离群体.遗传分析表明:该群体中黄籽性状受1对隐性主效基因控制;以法国甘蓝型油菜Darmor-bzh基因组为参考,利用集团分离分析法和全基因组重测序将黄籽性状基因定位于C03染色体6.1~9.0 Mb置信区间内,同时基于基因组变异和聚丙烯酰胺凝胶电泳验证获得与粒色基因紧密连锁的SSR标记4个和InDel标记5个.

关键词：甘蓝型油菜黄籽性状二代测序定位分子标记

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于抗坏血酸调控氧化铈纳米酶催化活性的抗坏血酸氧化酶检测研究

引用

分析科学学报 2024年第4期40卷 389-394页

作者：潘蕊黄伟华李佳娜王丽丽朱希许惠凤福建省中西医结合老年性疾病重点实验室福建中医药大学中西医结合研究院福建福州350002 福建中医药大学药学院福建福州350002 福建农林大学生命科学学院福建福州350002

纳米酶是一类既有纳米材料的独特性能又有催化功能的模拟酶,它既有天然酶的高催化活性,又有模拟酶稳定经济的特点,在生物传感检测领域的应用十分广泛。本研究采用水热法合成具有辣根过氧化物酶样活性的氧化铈纳米酶。该纳米氧化铈同时具... 详细信息

纳米酶是一类既有纳米材料的独特性能又有催化功能的模拟酶,它既有天然酶的高催化活性,又有模拟酶稳定经济的特点,在生物传感检测领域的应用十分广泛。本研究采用水热法合成具有辣根过氧化物酶样活性的氧化铈纳米酶。该纳米氧化铈同时具有+4价和+3价两种形态,其中Ce^(4+)能与过氧化氢反应,使3,3’,5,5’-四甲基联苯胺(TMB)氧化,溶液显蓝色。由于抗坏血酸(AA)能还原Ce^(4+),抑制其催化活性以及TMB的氧化,从而使溶液的颜色较浅。而抗坏血酸氧化酶可催化氧化AA,阻碍了其对Ce^(4+)的还原,使纳米氧化铈的催化性能恢复。利用这种方法,实现对抗坏血酸氧化酶的定量检测,在12 U/L~400 U/L范围内呈良好的线性关系(R^(2)=0.9704),检测限为3.2 U/L。该研究为基于氧化铈纳米酶的比色方法在抗坏血酸氧化酶活性检测及相关应用提供基础。

关键词：氧化铈抗坏血酸氧化酶纳米酶比色分析

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

引用

The Crop Journal 2020年第1期8卷 26-37页

作者： Yanmei Wang Shurong Jin Yi Xu Shuai li Shuangjuan Zhang Zheng Yuan jiana li Yu Ni College of Agronomy and Biotechnology Academy of Agricultural SciencesSouthwest University

Higher amounts of cuticular wax in plants have been associated with improved plant stress tolerance and increased potential for industrial *** this study,orthologs of KCS1 and CER1 in Arabidopsis,designated BnKCS1-1,BnKCS1-2,and BnCER1-2,were isolated from Brassica *** of BnKCS1-1 and BnKCS1-2 in *** were induced by abscisic acid(ABA)and drought treatment,while transcription of BnCER1-2 was induced only by drought *** three gene transcripts decreased significantly when plants were treated with methyl jasmonate(MeJA)or subjected to cold *** of BnKCS1-1,BnKCS1-2,and BnCER1-2 under the control of the CaMV35S promoter led to a significant increase in cuticular wax on transgenic *** ***1-1 and BnKCS1-2 overexpression led to similar differences from non-transformed plants,with significantly higher levels of aldehydes(C29 and C30),alkanes(C28,C29,and C31)and secondary alcohols(C28 and C29),and a significantly lower level of C29 *** of BnCER1-2 led to an increase in alkanes(C27,C28,C29,and C31),a decrease in secondary alcohols(C28 and C29),and insignificant changes in other wax *** electron microscopy revealed that overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 in *** resulted in a higher density of wax crystals on the leaf surface than observed in non-transformed *** plants showed a reduced rate of water loss and increased drought tolerance compared to non-transformed *** results suggest that BnKCS1-1,BnKCS1-2,and BnCER1-2 gene products can modify the cuticular wax of *** cuticular waxes using transgenic approaches is a new strategy for genetic improvement of plant drought tolerance and provides an opportunity for development of *** as a surface-wax crop.

关键词： Brassica napus Cuticular wax Drought tolerance Overexpression Transformation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

亚麻种质生氰糖苷含量测定与评价

引用

黑龙江农业科学 2024年第4期 81-85页

作者：苑志强李俊李志伟李佳娜李小萌伊六喜内蒙古农业大学科技园区/农学院内蒙古呼和浩特010019 内蒙古大学生命科学学院内蒙古呼和浩特010070

为进一步评价亚麻种质亚麻苦苷和百脉根苷含量的变异特征,本研究以20份亚麻种质为材料,利用高效液相色谱结合蒸发光检测仪测定亚麻苦苷和百脉根苷含量。结果表明,亚麻苦苷含量的均值为0.56±0.25 mg·kg^(-1),变异系数和遗传多样性指数... 详细信息

为进一步评价亚麻种质亚麻苦苷和百脉根苷含量的变异特征,本研究以20份亚麻种质为材料,利用高效液相色谱结合蒸发光检测仪测定亚麻苦苷和百脉根苷含量。结果表明,亚麻苦苷含量的均值为0.56±0.25 mg·kg^(-1),变异系数和遗传多样性指数分别为42.19%和31.26%;百脉根苷含量均值为0.25±0.24 mg·kg^(-1),变异系数和遗传多样性指数分别为60.67%和41.96%;亚麻籽亚麻苦苷、百脉根苷和二者总含量相互之间均极显著正相关。亚麻苦苷和百脉根苷含量与亚麻产量和品质相关性状的相关性分析表明,亚麻苦苷与棕榈酸之间显著负相关(-0.133),与其他脂肪酸相关性状之间不显著;亚麻籽亚麻苦苷、百脉根苷和二者总含量均与株高极显著正相关,其中与二者总含量的相关系数最大(0.216);聚类分析结果表明,遗传相似系数0.70时分为20个亚麻种质3个类群,第一类群种质的亚麻苦苷和百脉根苷平均含量较低,分别为0.46和0.11 mg·kg^(-1),这一类群中的内亚六号、H 140、内亚七号、内蒙红、CDC ARRAS、NOR MAN和晋亚8号这7个品种可以加以推广利用。

关键词：亚麻生氰糖苷变异系数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

New Insights into Nested Long Terminal Repeat Retrotransposons in Brassica Species

引用

Molecular Plant 2013年第2期6卷 470-482页

作者： lijuan Wei Meili Xiaoa Zeshan An Bi Ma Annaliese S. Mason Wei Qian jiana li Donghui Fu Chongqing Engineering Research Center for Rapeseed College of Agronomy and Biotechnology Southwest University Chongqing 400716 China School of Agriculture and Food Sciences and centre for Integrative I~egume-Research The University of Queensland 1:he Universit'y of QueenslandBrisbane 4072 Australia Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Agronomy College Jiangxi Agricultural University Nanchang330045 China

Long terminal repeat （LTR） retrotransposons, one of the foremost types of transposons, continually change or modify gene function and reorganize the genome through bursts of dramatic proliferation. Many LTR-TEs preferen-tially insert within other LTR-TEs, but the cause and evolutionary significance of these nested LTR-TEs are not well under-stood. In this study, a total of 1.52 Gb of Brassica sequence containing 2020 bacterial artificial chromosomes （BACs） was scanned, and six bacterial artificial chromosome （BAC） clones with extremely nested LTR-TEs （LTR-TEs density： 7.24/kb） were selected for further analysis. The majority of the LTR-TEs in four of the six BACs were found to be derived from the rapid proliferation of retrotransposons originating within the BAC regions, with only a few LTR-TEs originating from the proliferation and insertion of retrotransposons from outside the BAC regions approximately 5-23 Mya. LTR-TEs also pref-erably inserted into TA-rich repeat regions. Gene prediction by Genescan identified 207 genes in the 0.84Mb of total BAC sequences. Only a few genes （3/207） could be matched to the Brassica expressed sequence tag （EST） database, indicating that most genes were inactive after retrotransposon insertion. Five of the six BACs were putatively centromeric. Hence, nested LTR-TEs in centromere regions are rapidly duplicated, repeatedly inserted, and act to suppress activity of genes and to reshuffle the structure of the centromeric sequences. Our results suggest that LTR-TEs burst and proliferate on a local scale to create nested LTR-TE regions, and that these nested LTR-TEs play a role in the formation of centromeres.

关键词： LTR retrotransposons Brassica centromere retrotransposon-rich transposon burst.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

藜麦种质资源的茎秆性状评价

引用

山西农业大学学报（自然科学版） 2024年第4期44卷 1-9页

作者：张永康王树彦张锦豪李佳娜邢路虹刘瑞香郭占斌内蒙古农业大学农学院内蒙古呼和浩特010019 内蒙古农业大学沙漠治理学院内蒙古呼和浩特010011 内蒙古益稷生物科技有限公司内蒙古呼和浩特010011

[目的]藜麦种子具有全谷物高营养的品质,但倒伏是制约藜麦产量的重要因素之一,明确藜麦作物茎秆的抗倒伏特性,有利于筛选具有不同倒伏能力的藜麦品种(系),为藜麦抗倒伏育种鉴定、评价和创新提供理论依据。[方法]本研究根据株高、籽粒大... 详细信息

[目的]藜麦种子具有全谷物高营养的品质,但倒伏是制约藜麦产量的重要因素之一,明确藜麦作物茎秆的抗倒伏特性,有利于筛选具有不同倒伏能力的藜麦品种(系),为藜麦抗倒伏育种鉴定、评价和创新提供理论依据。[方法]本研究根据株高、籽粒大小、倒伏特性和生育期长短选取22份供试藜麦品种(系)并对其生育期、茎秆表型性状、茎秆机械强度和生理特征共16个指标进行测定和分析。[结果]22份藜麦品种(系)具有较高度的遗传变异,14个性状的变异系数为12.39%~81.08%,倒伏指数的变异系数最大,纤维素含量的变异系数最小。多因素方差分析得出作物倒伏特性与品种(系)、生育期、机械强度指标三者具有显著互作关系。相关性分析发现弯曲力矩、倒伏指数与茎秆表型性状、机械强度指标具有显著或极显著相关关系。基于13个茎秆特性指标以及隶属函数均值进行聚类分析可将22份藜麦品种(系)划分为3个类群,第Ⅰ类为抗倒伏组,第Ⅱ、Ⅲ类群为易倒伏组。多元线性回归分析得出,11个茎秆特性指标与倒伏指数和倒伏率做回归,R2均大于0.85。22份藜麦品种(系)的隶属函数均值为2.21~1.47,倒伏率范围为18.13%~91.83%,倒伏等级为2~5级。[结论]22份藜麦品种(系)农艺性状遗传多样性较为丰富,可为藜麦种质资源的创造提供参考。倒伏指数和弯曲力矩对作物倒伏能力评价具有重要作用。综合分析后推测K157、W120为抗倒伏品系,Y4、W26为易倒伏品系。

关键词：倒伏性茎秆特性倒伏指数弯曲力矩

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Broadening the genetic base of Brassica juncea by introducing genomic components from *** and *** via digenomic allohexaploid bridging

引用

The Crop Journal 2022年第3期10卷 672-679页

作者： Jiaqin Mei Jin liu Fang Yue Yangui Chen Jiayi Ming Zhiyong Xiong Fengqun Yu jiana li Wei Qian College of Agronomy and Biotechnology Southwest UniversityChongqing 400716China Academy of Agricultural Sciences Southwest UniversityChongqing 400716China State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Chongqing 400715China Key Laboratory of Herbage and Endemic Crop Biotechnology School of Life SciencesInner Mongolia UniversityHohhot 010070Inner Mongoli1China Agriculture and Agri-Food Canad1 Saskatoon Research and Development Center107 Science PlaceSaskatoonSK S7N 0X2Canada

A narrow genetic base has hindered improvement of Brassica juncea(A^(j)A^(j)B^(j)B^(j)).In this study,large-scale genomic components were introduced from diploid ancestor species into modern *** using a digenomic hexaploid *** hexaploids A^(j)A^(j)A^(r)A^(r)B^(j)B^(j) and A^(j)A^(j)B^(j)B^(j)B^(n)B^(n) were first developed from ***×***(A^(r)A^(r))and ***×***(B^(n)B^(n)),and then crossed with dozens of *** and ***,*** types of hexaploid showed high pollen fertility and moderate seed set throughout the S_(1) to S_(3) generations,and could be crossed with diploid progenitor species under field conditions,in particular for the combination of A^(j)A^(j)B^(j)B^(j)B^(n)B^(n)×*** A^(j)A^(r)B^(n)B^(j)-type and 31 A^(j)A^(r)B^(n)B^(j)-type *** resources were generated,of which the A^(j)A^(r)B^(n)B^(j) type showed higher *** these new-type *** resources,97 individual plants were genotyped with 42 simple sequence repeat markers,together with 16 current *** accessions and 30 hexaploid *** on 180 polymorphic loci,the new-type *** resources and current *** were separated clearly into distinct groups,with large genetic distance between the new-type *** resources and current *** study provides a novel approach to introducing large-scale genomic components from diploid ancestor species into ***.

关键词： Brassica juncea Diploid progenitor species Exotic introgression Genetic diversity Digenomic hexaploid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus

引用

The Crop Journal 2023年第3期11卷 756-765页

作者： Yingchao Tan Lanyang Ren Jia Wang Shuyao Ran liusha Wu Ziyi Cheng Cunmin Qu jiana li liezhao liu College of Agronomy and Biotechnology Southwest UniversityChongqing 400715China Chongqing Engineering Research Center for Rapeseed Academy of Agricultural SciencesState Cultivation Base of Crop Stress Biology for Southern Mountainous LandSouthwest UniversityChongqing 400715China

The leaf is the main organ for rapeseed photosynthesis,and its morphology influences photosynthetic efficiency and supports increased planting density and ***,the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood,restricting progress in breeding for the *** describe a novel dominant mutation,curly leaf 1(cl1),which confers uneven dorsal–ventral axis development,irregular cellular structure and influenced gravitropic response in the seedling *** CL1 locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat(SSR)markers,and co-segregated with the phenotype of plants in the curly F2 population.A substitution(P62S)was identified in the highly conserved degron motif(GWSPV)of the IAA2 protein in the cl1mutant,and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin,influencing auxin *** P62S substitution-induced curly leaf phenotype was verified by ectopic expression of Bna ***2 in Arabidopsis and *** findings explain the function of IAA2 in rapeseed,providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development in ***.

关键词： Brassica napus Curly leaf Auxin IAA2 protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a target capture sequencing SNP genotyping platform for genetic analysis and genomic breeding in rapeseed

引用

The Crop Journal 2023年第2期11卷 499-510页

作者： Xiaodong li Xumei liu Yonghai Fan Shengting li Mengna Yu Mingchao Qian Yuling Chen Hongqiao Chen Xinchun li Bei liu Xinfu Xu Cunmin Qu jiana li Kun Lu Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City and Southwest University College of Agronomy and BiotechnologySouthwest UniversityBeibeiChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Academy of Agricultural Sciences Southwest UniversityBeibeiChongqing 400715China China Golden Marker(Beijing)Biotech Co. Ltd.Beijing 102206China

Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding ***,the complexity of its polyploid genome increases sequencing costs and reduces sequencing *** capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of *** this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named *** platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic *** capture quality of BnaPan50T was demonstrated well in 12 typical *** with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide *** capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the ***50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification *** study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.

关键词： Rapeseed Target capture SNP genotyping platform Genomic breeding

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：