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World Journal of Gastroenterology 2006年第25期12卷 3983-3988页

作者： jian-Zheng Jie jian-wei wang jian-Guo Qu wei wang Tao Hung National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Department of General Surgery China-Japan Union Hospital Jilin University Changchun 130031 Jilin Province China

AIM： To investigate the inhibitory effects of a recombinant adenovirus vector that expresses NK4, a truncated form of human hepatocyte growth factor （HGF）, on human colonic adenocarcinoma cells in vitro to establish a basis for future NK4 gene cancer therapy. METHODS： Cells from the LS174T human colonic adenocarcinoma cell line were infected with recombinant adenovirus rvAdCMV/NK4 and the effects of the manipulation on tumor cell proliferation, scatter, migration, and basement membrane invasion were assessed. Cells infected with a recombinant adenovirus vector （Ad-LacZ） expressing β-galactosidase served as the controls. RESULTS： We found that rvAdCMV/NK4 expression attenuated HGF-induced tumor cell scatter, migration, and basement membrane invasion （P 〈 0.05）, but did not inhibit tumor cell proliferation. CONCLUSION： HGF-induced LS174T tumor cell scatter, migration, and invasion can be antagonized by the recombinant NK4-expressing adenovirus.

关键词： Human colonic adenocarcinoma NK4 Hepatocyte growth factor Adenoviral vector

Transurethral resection of the prostate stricture management

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Asian Journal of Andrology 2020年第2期22卷 140-144页

作者： jian-wei wang Li-Bo Man Department of Urology Beijing Jishuitan HospitalThe Fourth Medical College of Peking UniversityBeijing 100096China

For more than nine decades,transurethral resection of the prostate remains the gold standard for the surgical treatment of lower urinary tract symptoms due to benign prostatic *** occurrence of urethral strictures after transurethral resection of the prostate is one of the major late complications and has been reported as the leading cause of iatrogenic urethral strictures in patients older than 45 years who underwent *** several postulations have been proposed to explain the urethral stricture after transurethral resection of the prostate,the exact etiology of urethral stricture after TURP is still *** etiological factors of urethral stricture formation after transurethral resection of the prostate include infection,mechanical trauma,prolonged indwelling catheter time,use of local anesthesia,and electrical injury by a stray *** single treatment option is not appropriate for all stricture *** management of urethral stricture following transurethral resection of the prostate includes minimally invasive endoscopic methods,including urethral dilation and direct visual incision,or open surgical procedures with varying urethroplasty *** scientific studies focusing on urethral strictures after transurethral resection of the prostate are relatively limited and sparse,we can apply the principles of urethral stricture management before making decisions on individual stricture treatment.

关键词： benign prostatic hyperplasia transurethral resection of the prostate urethral stricture

Fitness of others'evaluation effect promotes cooperation in spatial public goods game

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Chinese Physics B 2021年第12期30卷 648-655页

作者： jian-wei wang Rong wang Feng-Yuan Yu School of Business Administration Northeastern UniversityShenyang 110819China

Payoff-driven strategy updating rule has always been adopted as a classic mechanism,but up to now,there have been a great many of researches on considering other forms of strategy updating rules,among which pursuing high fitness is one of the most direct and conventional motivations in the decision-making using game *** there are few or no researches on fitness from the perspective of others'*** view of this,we propose a new model in which the evaluation effect with fitness-driven strategy updating rule is taken into consideration,and introduce an evaluation coefficient to present the degree of others'evaluation on individual's *** cooperative individuals can get positive evaluation,otherwise defective individuals get negative evaluation,and the degree of evaluation is related to the number of neighbors who have the same strategy of *** numerical simulation,we find that the evaluation effect of others can enhance the network reciprocity,thus promoting the *** a strong dilemma,the higher evaluation coefficient can greatly weaken the cooperation dilemma;for a weak one,the higher evaluation coefficient can make cooperator clusters spread faster,however,there is no significant difference in the level of cooperation in the final stable state among different evaluation *** cooperation becomes more flourish as the number of fitness-driven individuals increases,when all individuals adopt fitness-driven strategy updating rule,the cooperators can quickly occupy the whole ***,we demonstrate the robustness of the results on the WS small-world network,ER random network,and BA scalefree network.

关键词： public goods game evaluation effect fitness network reciprocity

Hemodynamic changes in hepatic sinusoids of hepatic steatosis mice

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World Journal of Gastroenterology 2019年第11期25卷 1355-1365页

作者： Jing Fan Chong-Jiu Chen Yu-Chen wang wei Quan jian-wei wang wei-Guang Zhang Department of Anatomy Histology and EmbryologySchool of Basic Medical SciencesPeking University Health Science Center

BACKGROUND Fatty liver(FL) is now a worldwide disease. For decades, researchers have been kept trying to elucidate the mechanism of FL at the molecular level, but rarely involve the study of morphology and medical physics. Traditionally, it was believed that hemodynamic changes occur only when fibrosis occurs, but it has been proved that these changes already show in steatosis stage, which may help to reveal the pathogenesis and its progress. Because the pseudolobules are not formed during the steatosis stage, this phenomenon may be caused by the compression of the liver microcirculation and changes in the *** To understand the pathogenesis of hepatic steatosis and to study the hemodynamic changes associated with hepatic *** Eight-week-old male C57 BL/6 mice were divided into three groups randomly(control group, 2-wk group, and 4-wk group), with 16 mice per group. A hepatic steatosis model was established by subcutaneous injection of carbon tetrachloride in mice. After establishing the model, liver tissue from mice was stained with hematoxylin and eosin(HE), and oil red O stains. Blood was collected from the angular vein, and hemorheological parameters were estimated. A two-photon fluorescence microscope was used to examine the flow properties of red blood cells in the hepatic *** Oil red O staining indicated lipid accumulation in the liver after CCl_4 *** staining indicated narrowing of the hepatic sinusoidal vessels. No significant difference was observed between the 2-wk and 4-wk groups of mice onmorphological examination. Hemorheological tests included whole blood viscosity(mPas, γ = 10 s-1/γ = 100 s-1)(8.83 ± 2.22/4.69 ± 1.16, 7.73 ± 2.46/4.22 ±1.32, and 8.06 ± 2.88/4.22 ± 1.50), red blood cell volume(%)(51.00 ± 4.00, 42.00 ±5.00, and 40.00 ± 3.00), the content of plasma fibrinase(g/L)(3.80 ± 0.50, 2.90 ±0.80, and 2.30 ± 0.70), erythrocyte deformation index(%)(44.49 ± 5.81, 48.00 ±15.29, and 44.36 ± 15.01), eryt

关键词： Hepatic steatosis Hemodynamics Hepatic sinusoids Two-photon fluorescence microscopy Carbon tetrachloride

Synthesis and characterization of insulin-5-Fu conjugate,enabling insulin as multi-drug carrier via dendritic approach

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Chinese Chemical Letters 2007年第3期18卷 247-250页

作者： Juan Huang jian wei wang Tao Gong Zhi Rong Zhang Key Laboratory of Drug Targeting Ministry of Education West China School of Pharmacy Sichuan University Chengdu 610041 China

To enable insulin as multi-drug carrier, we designed and synthesized dendritic linker molecule bearing three 5-fluorouracil residues at the branch ends. The new conjugate showed excellent water solubility. The stabilities under different conditions were investigated. The results showed that the conjugate was a potential prodrug to release free 5-Fu.

关键词： Insulin 5-Fluorouracil Prodrug Characterization Stability

Mechanical properties and microstructure of rolled and electrodeposited thin copper foil

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Rare Metals 2016年第12期35卷 909-914页

作者： Xiang-Qian Yin Li-Jun Peng Saif Kayani Lei Cheng jian-wei wang wei Xiao Li-Gen wang Guo-Jie Huang State Key Laboratory of Nonferrous Metals & Processes General Research Institute for Nonferrous Metals Metal Materials Division Korea Institute of Materials Science

Commercial grade rolled and electrodeposited copper foils from Japan and China were selected, and their mechanical properties and microstructure were investigated. It was observed that there are notable differences in fracture strength, elongation at break and hydrophilicity between rol- led and electrodeposited copper foils. The rolled copper foils have higher tensile strength, lower ductility and larger static contact angle than electrodeposited copper foils. The rolled copper foils contain a [^-fiber texture, and the electrodeposited copper foils have random crystalline orientations. It was also observed that the rolled foils have packed grains, and elec- trodeposited foils have equiaxial grains. The uniform fine grain size and a few substructures of Japanese electrode- posited foils are major reasons for their higher elongation.

关键词： Copper foil Rolling Electrodepositing Texture Substructure

Sensitivity of Different Cytotoxic Responses of Vero Cells Exposed to Organic Chemical Pollutants and their Reliability in the Bio-toxicity Test of Trace Chemical Pollutants

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Biomedical and Environmental Sciences 2010年第3期23卷 219-229页

作者： TING-TING LIAO YAN-LING SHI jian-wei JIA AND LEI wang College of Environmental Science and Engineering State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China

Objective To find a sensitive cytotoxic response to reflect the bio-toxicity of trace organic pollutants, the sensitivity and reliability of morphological change and proliferation inhibition of Vero cells exposed to 2, 4, 6-trichlorophenol （TCP） and the leachate from products related to drinking water （PRDW） were compared, and the mechanism of the morphological change in Vero cells exposed to chemical pollutants was studied. Methods Vero cells were treated by different concentration of TCP and the leachate from PRDW. Methylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide （MTT） assay was carried out for proliferation inhibition. Bioluminescence method was carried out as another method to test the toxicity of TCP. Flow Cytometry assay was used to test cell Apoptosis and damage of cell-membrane. Results 0.25 mg/L TCP had an effect on cell morphology, and the proportion of morphologically changed cells increased with increasing TCP concentration. At low TCP concentrations, inhibition of cell proliferation did not seem to correlate to TCP concentration, and was negative when TCP concentration was 〈1.0 mg/L. After exposure to leachate from PRDW extracted at different temperatures, the percentage of morphologically changed cells increased with extracting temperature, but the inhibition of cell proliferation failed to reflect the correlation between extracting temperature and proliferation inhibition of Vero cells. Although the Sensitivity of bioluminescence method seems to be similar to morphological change in Veto cells, the bacterial in this method is not homologous enough with human body cells to reflect the toxicity to human body. These imply cell morphological change is a more sensitive and reliable method to reflect bio-toxicity of organic pollutants than proliferation inhibition. Flow cytometry analysis and cell rejuvenation experiments indicated cell membrane damage, which results in cell morphological change, was an early and sensitive cytotoxic response comparing w

关键词： Vero cell Morphological change Membrane damage Bio-toxicity test Drinking water

New strategy during complicated open appendectomy: Convert open operation to laparoscopy

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World Journal of Gastroenterology 2014年第31期20卷 10938-10943页

作者： Jin-Hui Zhu wei Li Kai Yu Jia Wu Yun Ji jian-wei wang Department of General Surgery and Laparoscopic Center The Second Affiliated Hospital Zhejiang University School of Medicine Department of General Surgery Zhejiang Provincial People’s Hospital Department of Oncology the Second Affiliated Hospital Zhejiang University School of Medicine

AIM: To introduce a new strategy during complicated open appendectomy - converting open operation to laparoscopy.

关键词： Complicated appendectomy Open Laparoscopy Conversion Complication

Late-onset mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome with mitochondrial DNA 3243A>G mutation masquerading as autoimmune encephalitis:A case report

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World Journal of Clinical Cases 2023年第14期11卷 3275-3281页

作者： jian-wei wang Xiao-Bo Yuan Hong-Fang Chen Department of Neurology Affiliated Jinhua HospitalZhejiang University School of MedicineJinhua 321000Zhejiang ProvinceChina Department of Neurology The First People's Hospital of YongkangJinhua 321300Zhejiang ProvinceChina

BACKGROUND Here,we present a unique case of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes(MELAS)syndrome,which initially appeared to be autoimmune encephalitis and was ultimately confirmed as MELAS with the mitochondrial DNA 3243A>G *** SUMMARY A 58-year-old female presented with acute-onset speech impediment and auditory hallucinations,symmetrical bitemporal lobe abnormalities,clinical and laboratory findings,and a lack of relevant prodromal history,which suggested diagnosis of autoimmune *** work-up,in conjunction with the patient’s medical history,family history,and lactate peak on brain lesions on magnetic resonance imaging,suggested a mitochondrial *** genome analysis revealed the m.3243A>G variant in the MT-TL1 gene,which led to a diagnosis of MELAS *** This case underscores the importance of considering MELAS as a potential cause of autoimmune encephalitis even if patients are over 40 years of age,as the symptoms and signs are atypical for MELAS syndrome.

关键词： MELAS Mitochondrial DNA mutation Encephalitis Case report

INFLUENCE OF MOLECULAR weiGHT OF CHITIN AND ITS THREE DERIVATIVES ON CRITICAL CONCENTRATION OF LYOTROPIC LIQUID CRYSTALLINE PHASE TRANSITION

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Chinese Journal of Polymer Science 2000年第1期18卷 15-17页

作者： Yan-ming Dong jian-wei wang Qing Yuan Department of Materials Science, Xiamen University, Xiamen 361005, China 厦门大学福建厦门

The critical concentration of lyotropic liquid crystalline phase transition for chitin derivatives was determined using a polarization microscope. The influence of molecular weight on critical concentration of liquid crystalline solution for chitin, chitosan, cyanoethyl chitosan and propionyl chitin successively increases as the chain rigidity decreases. Therefore it can be used as an indicator of the chain rigidity.

关键词： chitin chitosan lyotropic liquid crystalline polymer critical concentration molecular weight