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Intravascular ultrasound-guided “extended” reverse controlled antegrade and retrograde subintimal tracking technique using a cutting balloon for recanalizing chronic coronary total occlusion with a side branch

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Journal of Geriatric Cardiology 2019年第6期16卷 498-501页

作者： Yong-Tai GONG jian-qiang li li SHENG Dang-Hui SUN Yue li Department of Cardiology the First Affiliated Hospital of Harbin Medical University

Percutaneous coronary intervention (PCI) of chronic total occlusion (CTO) represents the most technically challenging procedure in contemporary interventional cardiology.[1] Blunt lesions and presence of proximal side branch are considered to be strong predictors of reduced technical success.[ 2,3] For such lesions, the antegrade approach may not be feasible or desirable, and the retrograde approach can be used as the initial crossing strategy. However, when treating the blunt CTO with a large side branch proximal to the occlusion, the side branch might be occluded after stent implantation if the retrograde guidewire passed the occluded segment through the subintimal space and re-entered into the true lumen at the opposite side of the side branch.[4] We reported a useful method to solve the above issue which utilizes intravascular ultrasound (IVUS) to guide “extended” reverse controlled antegrade and retrograde subintimal tracking (CART) technique with a cutting balloon.

关键词： Chronic total occlusion Cutting balloon Intravascular ultrasound Percutaneous coronary intervention Reverse controlled antegrade and retrograde tracking technique

Nest survival rate of Reeves＇s pheasant(Syrmaticus reevesii) based on artificial nest experiments

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Zoological Research 2017年第1期38卷 49-54页

作者： Xu Luo Yu-Ze Zhao Jing Ma jian-qiang li Ji-liang Xu School of Nature Conservation Beijing Forestry University Beijing 100083 China

To explore the nest survival rate of Reeves＇ s pheasant（Syrmaticus reevesii） and the nest-site factors that affect it, we conducted artificial nest experiments with reference to natural nests at Dongzhai National Nature Reserve（DNNR）, Henan Province and Pingjingguan, Hubei Province from April to June 2014 simulating the situation in its early and later breeding season. We also determined distance characteristics of the nest sites by Arc GIS 10.0. Nest survival models were constructed in Program MARK for data analysis. Results indicated that in the early breeding season, the apparent survival rate（ASR） in DNNR（52.4%） was significantly greater than that in Pingjingguan（13.5%）, and the ASR in the later breeding season in DNNR（26.7%） was not indistinctively correlated with Pingjingguan（3.2%）. The daily survival rate（DSR） in the later breeding season was 93.8% in DNNR and 92.0% in Pingjingguan, respectively. The DSRs were both negatively correlated with nest distance to forest edges and settlements. The DSR in Pingjingguan was positively correlated with nest distance to paths and negatively correlated with nest distance to water sources. However, the DSR in DNNR was negatively correlated with nest distance to paths but positively correlated with nest distance to water sources.

关键词： Reeves's pheasant SyrmaUcus reevesfi Nest survival rate Artificial nest experiments.

A novel treatment of refractory coronary embolism:thrombus aspiration catheter-assisted twisting wire technique

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Journal of Geriatric Cardiology 2020年第2期17卷 110-115页

作者： Yi-Lun ZOU jian-qiang li Yong-Tai GONG Dang-Hui SUN Yue li Department of Cardiology the First Affiliated Hospital of Harbin Medical University

Coronary embolism caused by the thrombus from guiding catheters is a rare and challenging complication,which may result in serious and fatal consequences due to the loss of blood flow of the coronary artery during percutaneous coronary intervention(PCI).Current strategies for the reduction of thrombus burden include pharmacological agents(typically glycoprotein IIb/IIIa platelet inhibitors),embolic protection devices(filters and distal balloon occlusion with aspiration),mechanical thrombectomy and manual or aspiration thrombectomy devices.[1]The above strategies are widely used as the first-line treatments,but have limitations for some refractory thrombus.A few techniques have been invented before to address this issue,[2–6]but they are not always effective or unavailable in some medical centers.

关键词： Complication Percutaneous coronary intervention Refractory thrombus embolism Thrombectomy

Landslide susceptibility assessment of the region affected by the 25 April 2015 Gorkha earthquake of Nepal

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Journal of Mountain Science 2016年第11期13卷 1941-1957页

作者： Amar Deep Regmi Megh Raj Dhital ZHANG jian-qiang SU li-jun CHEN Xiao-qing Key Laboratory of Mountain Hazards and Earth Surface Process Institute of Mountain Hazards and EnvironmentCASChengdu 610041China Central Department of Geology Tribhuvan UniversityKirtipur 44613Nepal

Nepal was hit by a 7.8 magnitude earthquake on 25^(th) April,*** main shock and many large aftershocks generated a large number of coseismic landslips in central *** have developed a landslide susceptibility map of the affected region based on the coseismic landslides collected from remotely sensed data and fieldwork,using bivariate statistical model with different landslide causative *** the investigation,it is observed that most of the coseismic landslides are independent of previous *** of 3,716 mapped landslides,we used 80% of them to develop a susceptibility map and the remaining 20% were taken for validating the model.A total of 11 different landslide-influencing parameters were *** include slope gradient,slope aspect,plan curvature,elevation,relative relief,Peak Ground Acceleration(PGA),distance from epicenters of the mainshock and major aftershocks,lithology,distance of the landslide from the fault,fold,and drainage *** success rate of 87.66% and the prediction rate of86.87% indicate that the model is in good agreement between the developed susceptibility map and theexisting landslides ***,lithology,slope angle and elevation have played a major role in triggering the coseismic mass *** susceptibility map can be used for relocating the people in the affected regions as well as for future land development.

关键词： Earthquake Himalaya Coseismic landslide Susceptibility bivariate statistical model Nepal

Removal of refractory thrombus by 5F child catheter in patients with subacute myocardial infarction

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Journal of Geriatric Cardiology 2019年第2期16卷 168-172页

作者： li SHENG jian-qiang li Dang-Hui SUN Yong-Tai GONG Jing-Yi XUE Yue li Department of Cardiology the First Affiliated Hospital of Harbin Medical University

No-reflow phenomenon is common in patients with acute coronary syndrome(ACS)after percutaneous coronary intervention(PCI),and it is strongly associated with adverse clinical *** studies have shown that heavy thrombus burden could predict the development of no-reflow in *** and mechanical thrombus aspiration catheters are widely used;however,there is still residual refractory thrombus left after the routine use of thrombectomy,especially in patients with subacute myocardial infarction(SMI).In our practice,child catheter aspiration has been used as a safe and effective approach to solve the residual thrombus in SMI *** described this technique in detail by a series of cases.

关键词： Percutaneous coronary intervention Refractory thrombus Subacute myocardial infarction Thrombus aspiration

Genetic Analysis of the P1 Region of Human Enterovirus 71 Strains and Expression of the 55 F StrainVP1 Protein

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Virologica Sinica 2012年第1期27卷 10-18页

作者： jian-qiang li Jun-jie Yang Xiu-juan Fan Zhen-peng Sun Yan Sun Huan li Zi-xin Meng Wei li Lanzou Institute of Biological Products Lanzhou 730070China

Enterovirus 71 （EV71） is a member of the Entero-virus genus of the Picomaviridae family and is the major cause of Hand, foot, and mouth disease （HFMD） in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP 1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by EliSA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.

关键词： EV71 Genetic analysis P1 region Expression VP1 protein

Programmed cell death protein-1 inhibitor combined with chimeric antigen receptor T cells in the treatment of relapsed refractory non- Hodgkin lymphoma: A case report

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World Journal of Clinical Cases 2021年第10期9卷 2394-2399页

作者： Zhi-Yun Niu li Sun Shu-Peng Wen Zheng-Rong Song lina Xing Ying Wang jian-qiang li Xue-Jun Zhang Fu-Xu Wang Department of Hematology Second Hospital of Hebei Medical UniversityHebei Key Laboratory of HematologyShijiazhuang 050000Hebei ProvinceChina Central Laboratory Hebei Senlang Biotechnology Co.Shijiazhuang 050000Hebei ProvinceChina

BACKGROUND Chimeric antigen receptor T cell(CART)therapy has benefited many refractory lymphoma patients,but some patients experience poor *** studies have shown that programmed cell death protein-1(PD-1)inhibitors can improve and prolong the therapeutic effect of CAR-T cell *** SUMMARY A 61-year-old male presented with 15-d history of diarrhea and lower-limb edema.A large mass was detected in the pelvis,and pathology indicated non-Hodgkin diffuse large B-cell *** three cycles of the R-CHOP chemotherapeutic regimen,the patient showed three subcutaneous nodules under the left armpit and both sides of the cervical *** examination of the nodules indicated DLBCL *** patient was diagnosed with relapsed and refractory diffuse large B-cell *** recommended CAR-T cell *** treatment,the patient’s T cell function and expression of immune detection points were *** of PD-1 was obviously increased(52.7%)on cluster of differentiation(CD)3+T *** PD-1 inhibitor(3 mg/kg)was infused prior to lymphodepleting chemotherapy with fludarabine and ***-CD19 T cells of 3×10^(6)/kg and CAR-CD22 T cells 1×10^(6)/kg were infused,*** therapeutic effect was significant,and the deoxyribonucleic acid copy numbers of CAR-CD19 T cells and CAR-CD22 T cells were ***,the patient has been disease-free for more than 12 *** This case suggests that the combination of PD-1 inhibitors and CAR-T cellsimproved therapeutic efficacy in B-cell lymphoma.

关键词： Chimeric antigen receptor T cell Programmed cell death protein 1 inhibitor Relapsed/refractory non-Hodgkin lymphoma Case report

Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3' End Sequence Characterization Following Cell Culture

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Virologica Sinica 2010年第3期25卷 213-224页

作者： jian-qiang li Jie CHENG Xi LAN Xue-rui li Wei li Xiang-ping YIN Bao-yu li Bin YANG Zhi-yong li Yun ZHANG Ji-xing liU Key laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China Lanzou Institute of Biological Products Lanzhou 730070 China

The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.

关键词： Cloning Complete genome Gastroenteritis virus (TGEV) Cell culture

Prokaryotic Expression and Potential Application of the Truncated PCV-2 Capsid Protein

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Virologica Sinica 2010年第2期25卷 86-97页

作者： Zhong-zi LOU Zhi-yong li Gang WANG jian-qiang li Xi LAN Xue-rui li Xiang-ping YIN Ji-xing liU Si-dang liU Animal Infectious Diseases Research Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture Key Laboratory of Veterinary Public Health of Ministry of Agriculture Lanzhou 730046 China College of Veterinary Medicine Shandong Agricultural University Taian 271018 China

Three pairs of specific primers were designed to amplify the F2-1, F2-2 and XF2-2 truncated sequences of ORF2 which encodes the capsid protein of porcine circovirus type 2 （PCV-2）. The F2-1 sequence had most of the NLS region of ORF2, but the F2-2 and XF2-2 genes had the NLS region deleted. Truncated genes were subcloned into pET-32a（＋） vectors to construct recombinant fusion expression vectors. The vectors were then transformed into Rosetta（DE3） E. coli and expressed by induction of IPTG. Expressed proteins were detected by western blotting and EliSA. The protein with best immunoreactivity was confirmed and selected, then utilized to inoculate SPF rabbits to prepare polyclonal antibodies. The protein and prepared polyclonal antibody were utilized to detect sera samples against PCV-2 from Shandong province and PCV-2 particles in PK-15 cells. In our study, three recombinant fusion proteins were successfully obtained, and the molecular weights of fusion proteins were 35.9 kDa, 33.6 kDa and 38.6 kDa respectively detected by SDS-PAGE. All of the proteins showed positive reaction with anti-PCV-2 antisera, and His-XF2-2 showed better immunoreactivity than the others. The protein of His-XF2-2 was coated as antigen in EliSA to detect the seroprevalence of PCV-2 in certain districts of Shandong province, the seropositivity rate was 27.7 % （73/264）. Specific fluorescence and positive signals for PCV-2 could be detected in PK-15 cells inoculated with PCV-2 with the participation of prepared antibodies against His-XF2-2 in IFA and IPMA. Experimental results indicated that the truncated PCV-20RF2 gene containing most of the NLS region was successfully expressed in E. coli, and His-XF2-2 was demonstrated to have better immunoreactivity with anti-PCV-2 antisera than the other two fusion proteins. His-XF2-2 and prepared polyclonal antibodies against it had a satisfactory capability in detecting PCV-2 infection.

关键词： Porcine circovirus type 2 Capsid protein Fusion expression Polyclonal antibodies Virus detection