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Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

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World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： jian-jing lin Tong Ning Shi-Cheng Jia Ke-Jia Li Yong-Can Huang Qiang Liu jian-Hao lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： Nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

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Triptolide Inhibits Expression of Inflammatory Cytokines and Proliferation of Fibroblast-like Synoviocytes Induced by IL-6/sIL-6R-Mediated JAK2/STAT3 Signaling Pathway

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Current Medical Science 2021年第1期41卷 133-139页

作者： jian-jing lin Ke TAO Nan GAO Hui ZENG De-li WANG Jun YANG jian WENG National&Local Joint Engineering Research Center of Orthopaedic Biomaterials Department of Bone and Joint SurgeryPeking University Shenzhen HospitalShenzhen518000China Arthritis Clinical and Research Center Peking University People’s HospitalBeijing100044China Department of Rheumatism and Immunology Peking University Shenzhen HospitalShenzhen518000China Department of Radiology Peking University Shenzhen HospitalShenzhen518000China

Triptolide,a component of the Chinese herb Tripterygium wilfordii Hook F,has been proved to be effective in the treatment of rheumatoid arthritis(RA).However,its underlying mechanisms on RA have not yet been well *** observed the inhibitory effect of triptolide on the expression of inflammatory cytokines and proliferation of fibroblast-like synoviocytes(FLS)induced by the complex of interleukin-6(IL-6)and the soluble form of the IL-6 receptor(sIL-6R).Furthermore,to clarify the underlying mechanisms,we treated FLS with the Janus-activated kinase 2(JAK2)inhibitor/signal transducer and activator of transcription 3(STAT3)activation blocker *** this study,immunohistochemical staining was used to identify vimentin(+)and CD68(−)in *** FLS proliferation was measured by cell proliferation assay,and the cell cycles were analyzed by flow ***,ELISA was used to detect the expression of the inflammatory factors in culture *** expression levels of p-JAK2,JAK2,p-STAT3 and STAT3 were investigated through Western blotting *** results showed that IL-6/sIL-6R significantly increased the cell proliferation and expression of inflammatory cytokines,including IL-6,interleukin-1β(IL-1β)and vascular endothelial growth factor(VEGF).Triptolide or AZD1480 inhibited the cell proliferation and inflammatory cytokine expression in IL-6/sIL-6R-stimulated FLS by suppressing JAK2/*** study suggested that the physiological effects of triptolide on RA were due to its contribution to the inhibition of the inflammatory cytokine expression and FLS proliferation by suppressing the JAK2/STAT3 signaling *** may provide an innovative insight into the effect of triptolide in preventing RA pathogenesis.

关键词： triptolide inflammatory cytokines proliferation fibroblast-like synoviocytes JAK2/STAT3

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Initial precursor reaction mechanism of CVD-HfC coating based on density functional theory

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Chinese Journal of Aeronautics 2024年第7期37卷 511-521页

作者： Yunzhou lin Yulei ZHANG Yanqin FU jian ZHANG jing’an KONG Ruicong CHEN State Key Laboratory of Solidification Processing Carbon/Carbon Composites Research CenterNorthwestern Polytechnical UniversityXi’an 710072China Henan Key Laboratory of High Performance Carbon Fiber Reinforced Composites Carbon Matrix Composites Research InstituteHenan Academy of SciencesZhengzhou 450046China

Recently,the preparation of ultra-high temperature HfC ceramic coating has gained significant attention,particularly through the application of the HfCl_(4)-CH_(4)-H_(2)-Ar system via Chemical Vapor Deposition(CVD),which has been found widely applied to C/C ***,an analysis of the reactions that occur in the initial stage of the CVD-HfC coating process is presented using Density Functional Theory(DFT)and Transition State Theory(TST)at the B3LYP/Lanl2DZ *** results reveal that HfCl4 can only cleave to produce hypochlorite,which will further react with methyl to synthesize intermediates to form *** to the analysis of the energy barrier and reaction constant,HfCl preferentially reacts with methyl groups to form complex adsorptive intermediates at 1573 *** a C—Hf bond production energy of 212.8 kcal/mol(1 kcal=4.18 kJ),the reaction rate constant of HfCl+CH is calculated to be 2.15×10^(-18) cm^(3)/s at 1573 ***,both the simulation and experimental results exhibit that the upward trend of reaction rate constants with temperature is also consistent with the deposition rate,indicating that the growth curve of the reaction rate constants tends to flatten *** proposed reaction model of the precursor’s decomposition and reconstruction during deposition process has significant implication for the process guidance.

关键词： Carbon composite HfC coating Chemical vapor deposition Density functional theory Precursor reaction mechanism

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MADRL-based UAV swarm non-cooperative game under incomplete information

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Chinese Journal of Aeronautics 2024年第6期37卷 293-306页

作者： Ershen WANG Fan LIU Chen HONG jing GUO lin ZHAO jian XUE Ning HE School of Electronic and Information Engineering Shenyang Aerospace UniversityShenyang 110136China College of Robotics Beijing Union UniversityBeijing 100101China School of Engineering Science University of Chinese Academy of SciencesBeijing 100049China College of Smart City Beijing Union UniversityBeijing 100101China

Unmanned Aerial Vehicles(UAVs)play increasing important role in modern *** this paper,considering the incomplete observation information of individual UAV in complex combat environment,we put forward an UAV swarm non-cooperative game model based on Multi-Agent Deep Reinforcement Learning(MADRL),where the state space and action space are constructed to adapt the real features of UAV swarm air-to-air *** multi-agent particle environment is employed to generate an UAV combat scene with continuous observation *** recently popular MADRL methods are compared extensively in the UAV swarm noncooperative game model,the results indicate that the performance of Multi-Agent Soft Actor-Critic(MASAC)is better than that of other MADRL methods by a large *** swarm employing MASAC can learn more effective policies,and obtain much higher hit rate and win *** under different swarm sizes and UAV physical parameters are also performed,which implies that MASAC owns a well generalization ***,the practicability and convergence of MASAC are addressed by investigating the loss value of Q-value networks with respect to individual UAV,the results demonstrate that MASAC is of good practicability and the Nash equilibrium of the UAV swarm non-cooperative game under incomplete information can be reached.

关键词： UAV swarm Reinforcement learning Deep learning Multi-agent Non-cooperative game Nash equilibrium

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Energy spectrum computed tomography multi-parameter imaging in preoperative assessment of vascular and neuroinvasive status in gastric cancer

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2511-2520页

作者： jing Wang jian-Cheng Liang Fa-Te lin Jun Ma Department of Radiology Pingluo County People's HospitalShizuishan 753400Ningxia Hui Autonomous RegionChina Department of Gastrointestinal Surgery Jiangsu Provincial People's HospitalNanjing 210029Jiangsu ProvinceChina

BACKGROUND Vascular and nerve infiltration are important indicators for the progression and prognosis of gastric cancer(GC),but traditional imaging methods have some limitations in preoperative *** recent years,energy spectrum computed tomography(CT)multiparameter imaging technology has been gradually applied in clinical practice because of its advantages in tissue contrast and lesion detail *** To explore and analyze the value of multiparameter energy spectrum CT imaging in the preoperative assessment of vascular invasion(LVI)and nerve invasion(PNI)in GC *** Data from 62 patients with GC confirmed by pathology and accompanied by energy spectrum CT scanning at our hospital between September 2022 and September 2023,including 46 males and 16 females aged 36-71(57.5±9.1)years,were retrospectively *** patients were divided into a positive group(42 patients)and a negative group(20 patients)according to the presence of LVI/*** CT values(CT40 keV,CT70 keV),iodine concentration(IC),and normalized IC(NIC)of lesions in the upper energy spectrum CT images of the arterial phase,venous phase,and delayed phase 40 and 70 keV were measured,and the slopes of the energy spectrum curves[K(40-70)]from 40 to 70 keV were *** Core Tip:To investigate the application value of multiparameter energy spectrum computed tomography(CT)imaging in the preoperative assessment of vascular and nerve infiltration in patients with gastric cancer(GC).The imaging data of GC patients were retrospectively analyzed to evaluate the accuracy and sensitivity of CT for identifying and quantifying vascular and nerve infiltration and for comparison with postoperative pathological *** purpose of this study was to verify the clinical feasibility and potential advantages of multiparameter energy spectrum CT imaging in guiding preoperative diagnosis and treatment decision-making and to provide a new imaging basis for improving the diagnostic accuracy

关键词： Tomography X-ray computer Energy spectrum computed tomography Gastric cancer Vascular invasion Nerve invasion Cross-sectional study

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Mechanical and damping performances of TPMS lattice metamaterials fabricated by laser powder bed fusion

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China Foundry 2024年第4期21卷 327-333页

作者： Yan-peng Wei Huai-qian Li jing-jing Han Ying-chun Ma Hao-ran Zhou jing-chang Cheng jian Shi Zhi-quan Miao Bo Yu Feng lin National Key Laboratory of Advanced Casting Technologies Shenyang Research Institute of Foundry Co.Ltd.CAMShenyang 110022China Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Key Laboratory of Space Physics Beijing 100076China

Lattice metamaterials based on three-period minimum surface(TPMS)are an effective means to achieve lightweight and high-strength materials which are widely used in various fields such as aerospace and ***,its vibration and noise reduction,and damping properties have not been fully ***,in this study,the TPMS structures with parameterization were designed by the method of surface migration,and the TPMS structures with high forming quality was manufactured by laser powder bed fusion(LPBF).The mechanical properties and energy absorption characteristics of the beam and TPMS structures were studied and compared by quasi-static *** modal shapes of the beam lattice structures and TPMS structures were obtained by the free modal analysis,and the damping properties of two structures were obtained by modal *** the two structures after heat treatment with the same porosity of 70%,the yield strength of the beam lattice structure reaches 40.76 MPa,elastic modulus is 20.38 GPa,the energy absorption value is 32.23 MJ·m^(-3),the damping ratio is 0.52%.The yield strength,elastic modulus,energy absorption value,and damping ratio of the TPMS structure are 50.74 MPa,25.37 GPa,47.34 MJ·m^(-3),and 0.99%,*** results show that TPMS structures exhibit more excellent mechanical properties and energy absorption,better damping performance,and obvious advantages in structural load and vibration and noise reduction compared with the beam lattice structures under the same porosity.

关键词： lattice metamaterials TPMS energy absorption damping laser powder bed fusion

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Endoscopic detection and diagnostic strategies for minute gastric cancer:A real-world observational study

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3529-3538页

作者： Xiao-Wei Ji Jie lin Yan-Ting Wang jing-jing Ruan jing-Hong Xu Kai Song jian-Shan Mao Department of Gastroenterology The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009Zhejiang ProvinceChina Department of Pathology The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009Zhejiang ProvinceChina

BACKGROUND Minute gastric cancers(MGCs)have a favorable prognosis,but they are too small to be detected by endoscopy,with a maximum diameter≤5 *** To explore endoscopic detection and diagnostic strategies for *** This was a real-world observational *** endoscopic and clinicopathological parameters of 191 MGCs between January 2015 and December 2022 were retrospectively *** discoverable opportunity and typical neoplastic features were emphatically *** All MGCs in our study were of a single pathological type,97.38%(186/191)of which were differentiated-type *** light endoscopy(WLE)detected 84.29%(161/191)of MGCs,and the most common morphology of MGCs found by WLE was ***-band imaging(NBI)secondary observation detected 14.14%(27/191)of MGCs,and the most common morphology of MGCs found by NBI was *** three MGCs were detected by indigo carmine third *** a well-demarcated border lesion exhibited a typical neoplastic color,such as yellowish-red or whitish under WLE and brownish under NBI,MGCs should be *** proportion with high diagnostic confidence by magnifying endoscopy with NBI(ME-NBI)was significantly higher than the proportion with low diagnostic confidence and the only visible groups(94.19%>56.92%>32.50%,Pline combined with a typical neoplastic color using nonmagnifying observation is sufficient for diagnosis of *** improves the endoscopic diagnostic confidence of MGCs.

关键词： Minute gastric cancer White light endoscopy Narrow-band imaging endoscopy Indigo carmine Magnifying endoscopy Detection Diagnosis

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Jetting of a near-wall cavitation bubble induced by another tandem bubble

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Journal of Hydrodynamics 2024年第3期36卷 444-456页

作者： Hui Han jing-zhu Wang jian-lin Huang Peng-bo Bai Yong-gang Chen Yi-wei Wang Key Laboratory for Mechanics in Fluid Solid Coupling Systems Institute of MechanicsChinese Academy of SciencesBeijing100190China School of Engineering Science University of Chinese Academy of SciencesBeijing100049China Guangdong Aerospace Research Academy Guangzhou511458China School of Future Technology University of Chinese Academy of SciencesBeijing100049China jianghuai Advance Technology Center Hefei230088China

Double bubbles near a rigid wall surface collapse to produce a significant jet impact,with potential applications in surface cleaning and ultrasonic ***,the dynamic behaviors of near-wall bubbles remain *** this study,we investigate the jetting of a near-wall bubble induced by another tandem *** define two dimensionless standoff distances,γ_(1),γ_(2),to represent the distances from the center of the near-wall bubble to the rigid wall and the center of controlling bubble to the center of the near-wall bubble,*** observations reveal three distinct jetting regimes for the near-wall bubble:transferred jetting,double jetting,and directed *** further investigate the jetting mechanism,numerical simulations are conducted using the compressibleInterFoam solver in the open-source framework of OpenFOAM.A detailed analysis shows that the transferred jet flow is caused by the pinch-off resulting from the axial contraction velocity at the lower end of the near-wall bubble being greater than the vertical contraction velocity,leading to a maximum jet velocity of 682.58 m/*** the case of double jetting,intense stretching between the controlling bubble and the wall leads to a pinch-off and a double jetting with a maximum velocity of 1096.29 m/*** directed jet flow is caused by the downward movement of the high-pressure region generated by the premature collapse of the controlling bubble,with the maximum jet velocity reaching 444.62 m/s.

关键词： Jetting of near-wall bubble controlling bubble impact velocity

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Study of primordial deuterium abundance in Big Bang nucleosynthesis

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Nuclear Science and Techniques 2024年第3期35卷 208-215页

作者： Zhi-lin Shen jian-Jun He Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China

Big Bang nucleosynthesis(BBN)theory predicts the primordial abundances of the light elements^(2) H(referred to as deuterium,or D for short),^(3)He,^(4)He,and^(7) Li produced in the early *** these,deuterium,the first nuclide produced by BBN,is a key primordial material for subsequent *** date,the uncertainty in predicted deuterium abundance(D/H)remains larger than the observational *** this study,the Monte Carlo simulation code PRIMAT was used to investigate the sensitivity of 11 important BBN reactions to deuterium *** found that the reaction rate uncertainties of the four reactions d(d,n)^(3)He,d(d,p)t,d(p,γ)^(3)He,and p(n,γ)d had the largest influence on the calculated D/H ***,the calculated D/H uncertainty cannot reach observational precision even with the recent LUNA precise d(p,γ)^(3) He *** the nuclear physics aspect,there is still room to largely reduce the reaction-rate uncertainties;hence,further measurements of the important reactions involved in BBN are still necessary.A photodisintegration experiment will be conducted at the Shanghai Laser Electron Gamma Source Facility to precisely study the deuterium production reaction of p(n,γ)d.

关键词： Big Bang nucleosynthesis Abundance of deuterium Reaction cross section Reaction rate Monte Carlo method

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Recent progress in self-propelled particles

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Journal of Hydrodynamics 2024年第1期36卷 61-77页

作者： Zhen-yu Ouyang jian-zhong lin Laboratory of Impact and Safety Engineering Ministry of EducationNingbo UniversityNingbo315201China Department of Mechanics State Key Laboratory of Fluid Power and Mechatronic SystemsZhejiang UniversityHangzhou310027China

Self-propelled particles are commonly found in a large number of planktonic organisms such as bacteria,fungi,and algae in nature,and researchers have taken a long interest in exploring their swimming mechanisms for more than a *** in the past 20 years,with the development of computational fluid dynamics and flow display technology,as well as the need for the design of synthetic self-propelled particles and micro-swimming devices,self-propelled particles have become the forefront and hotspot of current research in the field of fluid *** paper first introduces the swimming characteristics of common self-propelled particles,leading to a classic“squirmer”type self-propelled particle *** this basis,a systematic introduction and summary of the theoretical and numerical simulation research of“squirmer”will be ***,the main challenges and opportunities faced by the current research will be summarized.

关键词： Self-propelled particles multiphase flow hydrodynamic interaction

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