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Intestinal damage mediated by Kupffer cells in rats with endotoxemia

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World Journal of Gastroenterology 2002年第5期8卷 923-927页

作者： jian-PingGong chuan-XinWu DepartmentofGeneralSurgery TheSecondCollegeofClinicalMedicin&theSecondAffiliatedHospitalofChongqingUniversityofMedicalScience400010 DepartmentofGeneralSurgery TheSecondCollegeofClinicalMedicin&theSeco

AIM: To determine the in vivo effects of phagocytic blockadeof Kupffer cell (KC) on the release of proinflammatorycytokines in small intestinal lesion and on the integrity ofintestinal tract by using gadolinium chloride (GdCI3) duringearly ***: Wistar rats were divided into three groups: GroupA, rats were injected with endotoxin (F. coli O111:B4, a doseof 12 ***-1) only; Group B, rats were pretreatedintravenously with 25 mg of GdCl3 per kg 24 h are givenendotoxin; and Group C, sham operation on *** animalswere sacrificed 4 h after endotoxin *** portion ofthe rats of three groups, bile duct was cannulated, which thebile was collected externally. Morphological changes of ileumwere observed under light microscopy and electronicmicroscopy. The KC were isolated from rats by collagenaseperfusion and in KC, expression of TNF-α and IL-6 mRNAwere determined by RT-PCR analysis. Plasma and bile TNF-αand IL-6 Levels were determined by enzyme-linkedimmunosorbent assay (ELISA).RESULTS: In group A, there were neutrophil infiltrationand superficial epithelial necrosis of the ileal villi, sloughingof mucosal epithelium, and disappearance of some villi. Ingroup B, the ileal mucosal damage was much reduced. whichin group C, no significant morphological changes were ***3 pretreatment decreased significantly the expressionof TNF-α and IL-6 mRNA in group B (4.32±0.47 and 4.05±0.43) when compared to group A (9.46±1.21 and 9.04±1.09) (P＜0.05). There was no significant expression of TNF-α and IL-6 mRNA in group C (1.03±0.14 and 10.4±0.13).In rats of group A, the levels of TNF-α and IL-6 in bile andplasma were 207±29 ng. L-1, 1032±107 ng. L-1, 213±33ng. L-1, and 1185±127 ng. L-1, respectively. In group B, theywere 113±18 ng. L-1, 521±76 ng. L-1, 147±22 ng. L-1, and572±54 ng. L-1, respectively. In group C, they were 67±10ng. L-1, 72±13 ng. L-1, 109±18 ng. L-1, and 118±22 ng. L-1respectively. There were significant difference between thethree group (P＜O.05).

关键词：炎症细胞内毒素血症动物实验肠道狭窄 ELISA法

Fundamental mechanism of effects of MgO on sinter strength

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Journal of Iron and Steel Research International 2019年第11期26卷 1171-1177页

作者： Hong-song Han Feng-man Shen Xin jiang chuan-guang Bi Hai-yan zheng Qiang-jian Gao School of Metallurgy Northeastern UniversityShenyang 110819LiaoningChina Technology Center Meishan Iron and Steel Limited CorporationNanjing 210000JiangsuChina

MgO-containing flux may have a series of effects on the quality of sinter and performances of the blast ***,the fundamental mechanism of the effects of MgO on the sinter strength was *** the chemical reagent and industrial flux were used for preparing the *** experimental results show that the sinter strength decreases with MgO *** are three reasons for *** first reason is diffusion *** all of the CaO may react with Fe2O3 and generate CaO Fe2O3,but most of MgO cannot react with Fe2O3,and it still remains in the state of original *** diffusion rate of MgO in iron oxide is only 17.51μm/min in 30 *** second reason is the fluidity and ability to generate liquid *** the case of Fe2O3 mixed with CaO,there is some liquid phase formed above 1200℃,while in the case of Fe2O3 mixed with MgO,even at 1200 and 1220℃,there is still no liquid *** third reason is *** the case of industrial flux,the compression strength of the specimens made of Fe2O3 and limestone is 0.52 and 0.71 kN at 1150 and 1180℃,respectively,while that of the specimens made of Fe2O3 and magnesite is 0.48 and 0.56 kN,***,the fundamental mechanism of the effects of MgO additive on sinter strength can be better understood based on the diffusion rate of MgO in iron oxides,the fluidity of liquid phase,and the self-strength of bonding phase.

关键词： MgO addition Sinter strength Blast furnace Mineralization rate Fluidity Self-strength

Variable Universe Fuzzy Control for Battery Equalization

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Journal of Systems Science & Complexity 2018年第1期31卷 325-342页

作者： zheng jian CHEN jian OUYANG Quan The State Key Laboratory of Industrial Control Technology College of Control Science and Engineering Zhejiang University

In order to avoid the overcharge and overdischarge damages, and to improve the lifetime of the lithium-ion batteries, it is essential to keep the cell voltages in a battery pack at the same level,i.e., battery equalization. Based on the bi-directional modified Cuk converter, variable universe fuzzy controllers are proposed to adaptively maintain equalizing currents between cells of a serially connected battery pack in varying conditions. The inputs to the fuzzy controller are the voltage differences and the average voltages of adjacent cell pairs. A large voltage difference requires large equalizing current while adjacent cells both with low/high voltages can only stand small discharge/charge currents. Compared with the conventional fuzzy control method, the proposed method differs in that the universe can shrink or expand as the effects of the input changes. This is important as the input may change in a small range. Simulation results demonstrate that the proposed variable universe fuzzy control method has fast equalization speed and good adaptiveness for varying conditions.

关键词： Cell equalization lithium-ion battery variable universe fuzzy control

Multi-electron reaction and fast Al ion diffusion of δ-MnO_(2) cathode materials in rechargeable aluminum batteries via first-principle calculations

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Chinese Chemical Letters 2024年第4期35卷 248-254页

作者： Lumin zheng Ying Bai chuan Wu School of Materials Science&Engineering Beijing Institute of TechnologyBeijing 100081China Yangtze Delta Region Academy of Beijing Institute of Technology Jiaxing 314019China

Rechargeable aluminum batteries with multi-electron reaction have a high theoretical capacity for next generation of energy storage devices. However, the diffusion mechanism and intrinsic property of Al insertion into MnO_(2) are not clear. Hence, based on the first-principles calculations, key influencing factors of slow Al-ions diffusion are narrow pathways, unstable Al-O bonds and Mn^(3+) type polaron have been identified by investigating four types of δ-MnO_(2)(O3, O'3, P2 and T1). Although Al insert into δ-MnO_(2) leads to a decrease in the spacing of the Mn-Mn layer, P2 type MnO_(2) keeps the long(spacious pathways)and stable(2.007–2.030 A) Al-O bonds resulting in the lower energy barrier of Al diffusion of 0.56 e V. By eliminated the influence of Mn^(3+)(low concentration of Al insertion), the energy barrier of Al migration achieves 0.19 e V in P2 type, confirming the obviously effect of Mn^(3+) polaron. On the contrary, although the T1 type MnO_(2) has the sluggish of Al-ions diffusion, the larger interlayer spacing of Mn-Mn layer,causing by H_(2)O could assist Al-ions diffusion. Furthermore, it is worth to notice that the multilayer δ-MnO_(2) achieves multi-electron reaction of 3|e|. Considering the requirement of high energy density, the average voltage of P2(1.76 V) is not an obstacle for application as cathode in RABs. These discover suggest that layered MnO_(2) should keep more P2-type structure in the synthesis of materials and increase the interlayer spacing of Mn-Mn layer for providing technical support of RABs in large-scale energy storage.

关键词： Rechargeable aluminum batteries δ-MnO_(2) First-principles calculations Multi-electron reaction Diffusion mechanism

Proteomic profiling of early degenerative retina of RCS rats

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 878-889页

作者： Zhi-Hong Zhu Yan Fu chuan-Huang Weng Cong-jian Zhao zheng-Qin Yin Southwest Hospital/Southwest Eye Hospital Third Military Medical University Chongqing 400038 China Key Lab of Visual Damage and Regeneration ＆ Restoration of Chongqing Chongqing 400038 China

AIM： To identify the underlying cellular and molecular changes in retinitis pigmentosa （RP）. METHODS： Label-free quantification-based proteomics analysis, with its advantages of being more economic and consisting of simpler procedures, has been used with increasing frequency in modern biological research. Dystrophic RCS rats, the first laboratory animal model for the study of RP, possess a similar pathological course as human beings with the diseases. Thus, we employed a comparative proteomics analysis approach for in-depth proteome profiling of retinas from dystrophic RCS rats and non-dystrophic congenic controls through Linear Trap Quadrupole - orbitrap MS/MS, to identify the significant differentially expressed proteins （DEPs）. Bioinformatics analyses, including Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway annotation and upstream regulatory analysis, were then performed on these retina proteins. Finally, a Western blotting experiment was carried out to verify the difference in the abundance of transcript factor E2F1. RESULTS： In this study, we identified a total of 2375 protein groups from the retinal protein samples of RCS rats and non-dystrophic congenic controls. Four hundred thirty-four significantly DEPs were selected by Student＇s t-test. Based on the results of the bioinformatics analysis, we identified mitochondrial dysfunction and transcription factor E2F1 as the key initiation factors in early retinal degenerative process. CONCLUSION： We showed that the mitochondrial dysfunction and the transcription factor E2F1 substantially contribute to the disease etiology of RP. The results provide a new potential therapeutic approach for this retinal degenerative disease.

关键词： retinal degeneration proteomics mitochondrion E2F1 MaxQuant RCS rat

Diabetes mellitus is a risk factor of acute kidney injury in liver transplantation patients

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Hepatobiliary & Pancreatic Diseases International 2021年第3期20卷 215-221页

作者： Yu-Jia Wang jian-Hua Li Yi Guan Qiong-Hong Xie chuan-Ming Hao zheng-Xin Wang Division of Nephrology Huashan HospitalFudan UniversityShanghai 200040China Division of General Surgery Huashan HospitalFudan UniversityShanghai 200040China

Background: Diabetes mellitus has become an increasing global health burden with rapid growing prevalence. Patients with diabetes have higher susceptibility to acute kidney injury(AKI). Liver transplantation(LT) predisposes the kidney to injury. However, the association between diabetes and AKI in LT patients remains unclear. Methods: We conducted a retrospective cohort study examining risk factors for AKI in patients undergone orthotopic LT. Potential risk factors including baseline estimated glomerular filtration rate(e GFR), the model for end-stage liver disease(MELD) score, diabetes, hypertension and intraoperative blood loss were screened. The primary endpoint was AKI occurrence. Multivariate logistic regression was used to analyze the association between potential risk factors and AKI. Results: A total of 291 patients undergone orthotopic LT were included in the present study. Among them, 102 patients(35.05%) developed AKI within 5 days after LT. Diabetes was identified as an independent risk factor for AKI. Patients who developed AKI had worse graft function recovery and higher mortality within 14 days after LT compared to those who did not develop AKI. AKI patients with diabetes had a significant decline of e GFR within the first postoperative year, compared with patients who did not develop AKI and who developed AKI but without diabetes. Conclusions: Diabetes is an independent risk factor for AKI after orthotopic LT. AKI is associated with delayed graft function recovery and higher mortality in short-term postoperative period. Diabetic patients who developed AKI after LT experience a faster decline of e GFR within the first year after surgery.

关键词： Acute kidney injury Liver transplantation Diabetes Renal function

The Characterization of a Class of Quantum Markov Semigroups and the Associated Operator-Valued Dirichlet Forms Based on Hilbert W＊-Module

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Acta Mathematica Sinica,English Series 2013年第5期29卷 857-866页

作者： Lun chuan ZHANG Mao zheng GUO School of Information Science Renmin University of China School of Mathematical Science Peking University

In this paper, we introduce the concept of operator-valued quadratic form based on Hilbert W＊-module l2 A, and give a one to one correspondence between the set of positive self-adjoint regular module operators on l2 A and the set of regular quadratic forms, where A is a finite and a-finite von Neumann algebra. Furthermore, we obtain that a strict continuous symmetric regular module operator semigroup （Tt）t∈R＋ C L（l2 A） is Markovian if and only if the associated A-valued quadratic form is a Dirichlet form, where L（l2 A） is the yon Neumann algebra of all adjointable module maps on l2 A.

关键词： Hilbert W*-module quantum Markov semigroup operator-valued Dirichlet forms

Suppression of human colon tumor growth by adenoviral vector-mediated NK4 expression in an athymic mouse model

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World Journal of Gastroenterology 2007年第13期13卷 1938-1946页

作者： jian-zheng Jie State Key Laboratory ofMolecular Virology and Genetic Engineering Beijing 100730China Natinoal Institute for Viral Disease Control and Prevention ChinaCDC Beijing 100052 China Department of General Surgery China-JapanFriendship Hospital Beijing 100029 China

AIM： To investigate the suppressive effects of adenoviral vector-mediated expression of NK4, an antagonist of hepatocyte growth factor （HGF）, on human colon cancer in an athymic mouse model to explore the possibility of applying NK4 to cancer gene therapy. METHODS： A human colon tumor model was developed by subcutaneous implantation of tumor tissue formed by LS174T cells grown in athymic mice. Fifteen tumorbearing mice were randomized into three groups （n= 5 in each group） at d 3 after tumor implantation and mice were injected intratumorally with phosphate-buffered saline （PBS） or with recombinant adenovirus expressing 13-galactosidase （Ad-LacZ） or NK4 （rvAdCMV/NK4） at a 6-d interval for total 5 injections in each mouse. Tumor sizes were measured during treatment to draw a tumor growth curve. At d 26 after the first treatment, all animals were sacrificed and the tumors were removed to immunohistochemically examine proliferating cell nuclear antigen （PCNA）, microvessel density （represented by CD31）, and apoptotic cells. In a separate experiment, 15 additional athymic mice were employed to develop a tumor metastasis model by intraperitoneal injection （ip） of LS174T cells. These mice were randomized into 3 groups （n = 5 in each group） at d 1 after injection and were treated by ip injection of PBS, or Ad-LacZ, or rvAdCMV/NK4 at a 6-d interval for total two injections in each mouse. All animals were sacrificed at d 14 and the numbers and weights of disseminated tumors within the abdominal cavity were measured. RESULTS： Growth of significantly suppressed human colon tumors were in the athymic mice treatedwith rvAdCMV/NK4 （2537.4± 892.3 mm^3） compared to those treated by either PBS （5175.2 ± 1228.6 mm^3） or Ad-LacZ （5578.8± 1955.7 mm^3）（P 〈 0.05）. The tumor growth inhibition rate was as high as 51%. Immunohistochemical staining revealed a similar PCNA labeling index （75.1% ± 11.2% in PBS group vs 72.8% ± 7.6% in Ad-LacZ group vs 69.3% ± 9.4% in rvAdCMV/ NK4 group） in all

关键词： Human colon cancer NK4 Hepatocytegrowth factor Adenoviral vector Gene therapy

Novel deletion mutations of the PIH1D3 gene in an infertile young man with primary ciliary dyskinesia and his cousin with Kartagener’s syndrome

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Asian Journal of Andrology 2021年第3期23卷 330-332页

作者： chuan Huang Na-chuan Liu Xiao-Bo Wang Ben-Hong Gu jian-Xiong Zhang Li-Zhang zheng Li Department of Andrology Center for Men's HealthShanghaiChina Department of ART Institute of Urologic Medical CenterShanghai General HospitalShanghaiKey Lab of Reproductive MedicineShanghai Jiao Tong UniversityShanghai 200080China The Human Sperm Bank Reproductive and Genetic Hospital of CITIC-XiangyaChangsha 410000China Department of Urology Tongren HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 200080China.

Dear Editor,Male infertility is defined as the inability to achieve pregnancy in a fertile female partner even after 12 months of unprotected *** humans,it accounts for 40%-50%of cases of infertility,1 and it occurs most often due to problems with sperm production or sperm *** infertility is commonly due to deficiencies in semen,and semen quality is used as a surrogate measure of male *** ciliary dyskinesia(PCD)is a rare cause of male infertility.2,3 It is an autosomal recessive genetic condition that causes abnormal function of *** are microscopic finger-like projections on the surface of the cells.

关键词： partner protected cilia