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Chinese Chemical Letters 2007年第6期18卷 677-680页

作者： Yu Cai he jian he xu Yi xu Li Ming Ouyang jiang Pan Laboratory of Biocatalysis and Bioprocessing State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China

By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp. ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mand... 详细信息

关键词： Mandelonitrile Nitrilase (R)-()-Mandelic acid Screening Alcaligenes sp. ECU0401

Asymmetric reduction of α-hydroxy aromatic ketones to chiral aryl vicinal diols using carrot enzymes system

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Chinese Chemical Letters 2012年第6期23卷 635-638页

作者： Xiang Liu Yi Wang Hai Yan Gao jian he xu a School of Chemical and Material Engineering Jiangnan University Wuxi 214122 China State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China

Asymmetric reduction of ct-hydroxy aromatic ketones was carded out by using carrot enzymes system, yielding corresponding chiral vicinal diols with special functional groups. The optimum reaction conditions were obtained after investigation of various influencing factors. Chiral aryl vicinal diols were produced with good yields and excellent enantiomeric excesses under appropriate conditions. Meanwhile, the steric factors and electronic effects of the substituents on the aromatic ring were shown to have an interesting influence on both yield and enantioselectivity.

关键词： Asymmetric reduction Chiral aryl vicinal diol Aromatic ketone Carrot cell

Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica

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Synthetic and Systems Biotechnology 2023年第2期8卷 331-338页

作者： Man xu Wenliang Xie Zhen Luo Chun-Xiu Li Qiang Hua jian-he xu State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for Biomanufacturing and School of BiotechnologyEast China University of Science and Technology130 Meilong RoadShanghai200237China

Taxadiene is an important precursor for the biosynthesis of highly effective anticancer drug paclitaxel,but its microbial biosynthesis yield is very *** this study,we employed Yarrowia lipolytica as a microbial host to produce ***,a“push–pull”strategy was adopted to increase taxadiene production by 234%.Then taxadiene synthase was fused with five solubilizing tags respectively,leading a maximum increase of 62.3%in taxadiene production when fused with ***,a multi-copy iterative integration method was used to further increase taxadiene titer,achieving the maximum titer of 23.7 mg/L in shake flask culture after three rounds of ***,the taxadiene titer was increased to 101.4 mg/L by optimization of the fed-batch fermentation *** is the first report of taxadiene biosynthesis accomplished in ***,serving as a good example for the sustainable production of taxadiene and other terpenoids in this oleaginous yeast.

关键词： Taxadiene Yarrowia lipolytica “Push–pull”ostrategy Multi-copy integration Fed-batch fermentation

Microwave-assisted synthesis of α-hydroxy aromatic ketones from α-bromo aromatic ketones in water

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Chinese Chemical Letters 2011年第1期22卷 53-56页

作者： Xiang Liu Hai Bo Chen Zheng Guang Pan jian he xu he Xing Li School of Chemical and Material Engineering Jiangnan University Wuxi 214122 China State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China Department of Chemistry Shanghai Normal University Shanghai 200234 China

A reaction of α-bromo aromatic ketones in water with microwave irradiation gave the corresponding α-hydroxy aromatic ketones in good yields. The use of microwaves was found to significantly improve yields and shorten the reaction time. This reaction afforded a very clean, convenient method for the synthesis of α-hydroxy aromatic ketones.

关键词： α-Hydroxy aromatic ketones α-Bromo aromatic ketones Microwave irradiation

Correction to:Effect of Process Parameters on Defects,Melt Pool Shape,Microstructure,and Tensile Behavior of 316L Stainless Steel Produced by Selective Laser Melting

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Acta Metallurgica Sinica(English Letters) 2021年第4期34卷 511-513页

作者： Hua-Zhen jiang Zheng-Yang Li Tao Feng Peng-Yue Wu Qi-Sheng Chen Yun-Long Feng Long-Fei Chen Jing-Yu Hou he-jian xu School of Engineering Science University of Chinese Academy of SciencesBeijing100190China Institute of Mechanics Chinese Academy of SciencesBeijing100190China Beijing E-Plus-3D Technology Co. LTDBeijing102299China

In the Original Publication of the article,some reference numbers in the Figs.1,5,14 and Appendixes B,C,D are *** corrected reference numbers in the figures and appendixes are given below.

关键词： Microstructure Defects 316L

Biosensor Based HTS Method for Molecular Evolutionary of the Organophosphate Hydrolase

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Biosensor Based HTS Method for Molecular Evolutionary of the...

第十一届中国酶工程学术研讨会

作者：程欢 Yunpeng Bai Chunxiu Li jian-he xu State Key laboratory of Bioreactor Engineering East China University of Science and Technology

Organophosphates(OPs) are highly toxic synthetic compounds and are widely used as pesticides,plasticizers and flame *** enzyme were reported to degrade these compounds,such as OPH,PTE and PON1(Gerhard et al,2016,Bai et al,2017).A newly reported organophosphate hydrolase,***,was discovered fromPseudomonas oleovorans,by simple double mutantions His250 Ile/Ile263 Trp,switching into an efficient and thermostable phosphotriesterase(Luoet al,2014).In order to exploxit this enzyme full potential of pesticides biodgradation,efforts have been made to *** to obtain the variants with higher hydrolysis *** based scereening is a high-throughput scereening method,which may be more effectively in finding phenotypes of interest(Rameshet al,2014).In this study,we have constructed the biosensor plasimid pET-28 a(+)-PoOPH-DSF for sensing the pesticides methyl parathion(limit of detection is 2μM),establishing correlation between hydrolysis rate of PoOPH and expression rate of sfGFP Using fluorescence-activated cell sorting(FACS),individual cells carrying the active variant PoOPH could be isolated even when diluted into a decoy population of cells carrying PoOPH wild type at one part in a *** on the HTS method,better mutant with higher catalytic efficiency(k/K) towards methyl parathionwas selected.

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A 3D-QSAR and Virtual Screening Assisted Activity Predicting Strategy for Substrate Spectrum Expansion of Aldehyde Ketone Reductase

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A 3D-QSAR and Virtual Screening Assisted Activity Predicting...

第十一届中国酶工程学术研讨会

作者：程强 Qi Chen jian-he xu Hui-lei Yu State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for BiomanufacturingEast China University of Science and Technology

Aldo-keto reductase（AKR） is widely used in reducing prochiral aldehyde or ketone compounds into corresponding chiral *** our previous work,an aldo-keto reductase named YtbE was isolated and identified from Bacillus ***0013,which can catalyze the NADPH-dependent carbonylreduction reaction with high ***,the reported detailed catalytic mechanism of YtbE is not clear and the substrate spectrum needs to be further *** address this issue,3-Dimensional Quantitative Structure Activity Relationship（3 D-QSAR） models of YtBE were constructed to predict the specific activity on more useful *** CoMFA and CoMSIA models,both the cross validation coefficient（Q2）（0.562,0.629）and non-cross validation coefficient（R2）（0.986,0.976） were satisfied,which prove the models have a robust and powerful predictive *** detailed relationship between the protein YtBE and substrates were then examined by the contour maps ***,virtual screening through the ZINC 15 database was used to expand the substrate spectrum of *** the candidate substrates,five of them were experimentally verified,indicating the accuracy of the moleculeCoMSIA ***3 D-QSAR assisted method was proved to be helpful for rational investigating and expanding the substrate spectrum of *** work also provides essential molecular insights into the substrate binding mechanism of YtbE.

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Molecular Engineering of an ε-Keto Ester Reductase for the Efficient Synthesis of an(R)-α-Lipoic Acid Precursor

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Molecular Engineering of an ε-Keto Ester Reductase for the ...

第十一届中国酶工程学术研讨会

作者：许尧 Qi Chen Gao-Wei Zheng jian-he xu State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for BiomanufactureEast China University of Science and Technology

Alpha-lipoic acid is a kind of potent antioxidant,but only the R form shows biological ***(R)-8-chloro-6-hydroxyoctanoate(precursor of R-alpha-lipoic acid)can be obtained by asymmetric reduction of ethyl 8-chloro-6-oxooctanoate(ECOO)by reductaseCpAR2 in high space-time yield(530 gLd) andwith excellent enantiomeric excess(>99%)(Yu-Jun Zhang,2015).In this work,we will improve the thermostability and activity of CpAR2 by protein engineering for a broad developing prospect for industrial *** spots for enhancing activity and thermostability were discovered through epPCR-based random mutagenesis,followed by site-saturation mutagenesis,and these beneficial mutations were then *** best variant CpAR2 showed 2-fold higher activity toward ethyl 8-chloro-6-oxooctanoate(ECOO,259.6 U/mg protein) than wild-type(120 U/mg protein),which is in the entrance *** variant CpAR2enhanced the Tby 4.2℃ over wild-type and Q252 is located in the loop of the enzyme surface.

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Cofactor specificity switch in 7β-Hydroxysteroid Dehydrogenase by Small-and-Smart Library Design

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Cofactor specificity switch in 7β-Hydroxysteroid Dehydrogen...

中国生物工程学会第十二届学术年会暨2018年全国生物技术大会

作者：游智能 Qi Chen Chun-Xiu Li jian-he xu State Key Laboratory of Bioreactor Engineering East China University of Science and Technology

Ursodeoxycholic acid(UDCA) is an active pharmaceutical ingredient(API) for dissolving cholesterol gallstones and improving the liver function in cholestatic diseases. It is also a FDA-approved therapeutic agent for pr...

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A newly developed thermostable amine dehydrogenase for synthesis of chiral amines

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A newly developed thermostable amine dehydrogenase for synth...

中国生物工程学会第十一届学术年会暨2017年全国生物技术大会

作者： Lei Liu Fei-Fei Chen jian-he xu Gao-Wei Zheng State Key laboratory of Bioreactor Engineering East China University of Science and Technology

Chiral amines are constitute of many pharmaceutical *** asymmetric reductive amination of ketone with ammonia is one of the most attractive ways to synthesize chiral ***,Bommarius and coworkers firstly developed amine dehydrogenases from amino acid dehydrogenases by protein engineering. However,the poor thermostability and narrow substrate scope limit their utilization in organic *** recently,Ursula has obtained a thermostable engineered amine dehydrogenase from Caldalkalibacillus thermarum,but the substrate scope is the same with other amine dehydrogenases ***,Identification of novel amine dehydrogenases with robust stability and broden substrate scope is still desired

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