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LC-MS-based lipidomic analysis in distinguishing patients with nonalcoholic steatohepatitis from nonalcoholic fatty liver

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Hepatobiliary & Pancreatic Diseases International 2021年第5期20卷 452-459页

作者： Zhong-Hua Wang Kenneth I Zheng Xiao-Dong Wang Jin Qiao Yang-Yang Li Li zhang Ming-Hua Zheng jian Wu Department of Medical Microbiology and Parasitology MOE/NHC/CAMS Key Laboratory of Medical Molecular VirologySchool of Basic Medical SciencesFudan University Shanghai Medical CollegeShanghai 200032China NAFLD Research Center Department of HepatologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Institute of Hepatology Wenzhou Medical UniversityWenzhou 325000China The Key Laboratory of Diagnosis and Treatment for the Development of Chronic Liver Disease in Zhejiang Province Wenzhou 325000China Department of General Practice Huaihai Middle Road Community Health Service Center of Huangpu DistrictShanghai 200025China Department of Pathology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Department of Gastroenterology and Hepatology Zhongshan Hospital of Fudan UniversityShanghai 200032China Laboratory of Fatty Liver and Metabolic Diseases Shanghai Institute of Liver DiseasesFudan University Shanghai Medical CollegeShanghai 200032China

Background: Nonalcoholic fatty liver disease(NAFLD) is one of the main liver diseases, and its pathologic profile includes nonalcoholic fatty liver(NAFL) and nonalcoholic steatohepatitis(NASH). However, there is no reliable non-invasive parameter in distinguishing NASH from NAFL in clinical practice. The present study was to find a non-invasive way to differentiate these two categories of NAFLD via lipidomic analysis. Methods: Lipidomic analysis was used to determine the changes of lipid moieties in blood from 20 NAFL and 10 NASH patients with liver biopsy. Liver histology was evaluated after hematoxylin and eosin staining and Masson’s trichrome staining. The profile of lipid metabolites in correlation with steatosis, inflammation, hepatocellular necroptosis, fibrosis, and NAFLD activity score(NAS) was analyzed. Results: Compared with NAFL patients, NASH patients had higher degree of steatosis, ballooning degeneration, lobular inflammation. A total of 434 different lipid molecules were identified, which were mainly composed of various phospholipids and triacylglycerols. Many lipids, such as phosphatidylcholine(PC)(P-22:0/18:1), sphingomyelin(SM)(d14:0/18:0), SM(d14:0/24:0), SM(d14:0/22:0), phosphatidylethanolamine(PE)(18:0/22:5), PC(O-22:2/12:0), and PC(26:1/11:0) were elevated in the NASH group compared to those in the NAFL group. Specific analysis revealed an overall lipidomic profile shift from NAFL to NASH, and identified valuable lipid moieties, such as PCs [PC(14:0/18:2), PE(18:0/22:5) and PC(26:1/11:0)] or plasmalogens [PC(O-22:0/0:0), PC(O-18:0/0:0), PC(O-16:0/0:0)], which were significantly altered in NASH patients. In addition, PC(14:0/18:2), phosphatidic acid(18:2/24:4) were positively correlated with NAS;whereas PC(18:0/0:0) was correlated positively with fibrosis score. Conclusions: The present study revealed overall lipidomic profile shift from NAFL to NASH, identified valuable lipid moieties which may be non-invasive biomarkers in the categorization o

关键词： Lipidomics Nonalcoholic steatohepatitis Nonalcoholic fatty liver disease Biomarker Nonalcoholic activity score Hepatic fibrosis

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Detection and identification for nematode Gnathostoma in imported Monopterus albus

Detection and identification for nematode Gnathostoma in imp...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Li Shuqing~1,Li Wenwen~2,Chen Zhifei~1,Li jian~2,Chen Shaohong~3,zhang Yongnian~3 Huang Weiyi~2 Wang Qiaoquan~1 1.Shanghai Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of china,Shanghai 200135 2.College of Animal science and technology,Guangxi university,Nanning 530005 3.Chinese Center for Disease Control and Prevention,National Institute of Parasitic Diseases, Shanghai 200025

Objective To inspect the third stage larvae of Gnathostoma in imported Monopterus albus,and identify its species..Method 10 batches of imported Monopterus albus to Shanghai from abroad were detected for nematode Gnathostoma from January 2010 to March *** 52 Monopterus albus,3～10/batch,From Philippines(25),Indonesia(24) and Bangladesh(3),dissect each Monopterus albus,then minced,protease digestion,the suspension was filtered with 10 mesh copper screen,at the last take the precipitation of the *** the complete parasites under the stereoscope,morphological identification,and calculate the infection rate and average *** extracted genomic DNA of Gnathostoma,amplified internal transcribed spacer region 2 and cytochrome c oxidase subunit 1 by PCR,then Gel electrophoresis and *** sequences were aligned with corresponding sequences in *** The third stage larvas of nematode Gnathostoma were detected in Monopterus albus from Indonesia and Philippines,infection rates were 36.0%(9/25) and 50.0% (12/24),average infectiosity were 7.77(70/9) and 2.83(34/12).There wasn’t Gnathostoma in imported Monopterus albus from *** the microscope,the larvas have one cephalic bulb,4 rings hooklets on the cephalic bulb,the body surface with cross striations and small spines,the front of the body spines were obviously and density,the rear spines getting small and *** had 1 cervical papillae,4 cervical *** characteristics was similar to Gnathostoma *** results showed,ITS-2 and cox1 PCR products were 647 bp and 441 bp,with the expected *** alignment analysis showed that,two PCR products had 99%～100%consistency with Gnathostoma spinigerum ITS-2(GenBank accession number AB181155 and Z97175) and coxl (GenBank accession number AY501388,AB180099 and AB551552).Conclusion All of the Gnathostoma detected in imported Monopterus albus from Philippines and Indonesia were Gnathostoma spinigerum.

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Robustness measurement of scale-free networks based on motif entropy

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Chinese Physics B 2022年第8期31卷 190-196页

作者： Yun-Yun Yang Biao Feng Liao zhang Shu-Hong Xue Xin-Lin Xie jian-Rong Wang College of Electrical and Power Engineering Taiyuan University of TechnologyTaiyuan 030024China Taiyuan university of science and technology Taiyuan 030024China school of Mathematical sciences Shanxi UniversityTaiyuan 030024China

As a classical complex network model, scale-free network is widely used and studied. And motifs, as a high-order subgraph structure, frequently appear in scale-free networks, and have a great influence on the structural integrity, functional integrity and dynamics of the networks. In order to overcome the shortcomings in the existing work on the robustness of complex networks, only nodes or edges are considered, while the defects of high-order structure in the network are *** the perspective of network motif, we propose an entropy of node degree distribution based on motif to measure the robustness of scale-free networks under random attacks. The effectiveness and superiority of our method are verified and analyzed in the BA scale-free networks.

关键词： motif network robustness scale-free entropy

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Denaturation behavior of inclusions in industrial pure iron by calcium treatment

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Journal of Iron and Steel Research International 2023年第2期30卷 249-258页

作者： Xin Liu Yan-ping Bao Cheng-jian Hua jian-hua Chu Chao Gu Min Wang State Key Lab of Advanced metallurgy University of Science and Technology BeijingBeijing100083China

Only a few Chinese enterprises can produce high-quality industrial pure iron,and studies on the smelting process of industrial pure iron were *** inclusions in a melting process were characterized by means of electron microscopy and an automatic inclusion analysis system,and the evolution mechanism of inclusion was studied using thermodynamic calculation to optimize the calcium alloy addition in liquid *** results show that during the smelting process,inclusions mainly composed of Al_(2)O_(3)and spinel are formed before calcium *** calcium treatment,they continuously react with[Ca],[S]and[Ti],grow up,and are removed during refining and tundish *** the end,there are more small-sized inclusions containing CaS,and the contents of Al_(2)O_(3)and spinel are *** to thermodynamic calculations,the appropriate calcium treatment liquid window for the molten steel composition is(10-38)×10^(-6).Calcium treatment has changed the main types of inclusions in industrial pure iron from Al_(2)O_(3)to small-sized inclusions containing CaS and effectively reduces the influence of Al_(2)O_(3)inclusions on the quality of industrial pure iron.

关键词： Industrial pure iron Calcium treatment Inclusion Thermodynamic CaS

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江浙皖部分地区副鸡禽杆菌分离鉴定及生物学特性研究

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动物医学进展 2024年第5期45卷 39-45页

作者：吴娴毕海洋张燕茹李向臣何玉龙舒建洪武永淑浙江理工大学生命科学与医药学院浙江杭州310018 浙江理工大学绍兴生物医药研究院浙江绍兴312366 浙江农林大学动物科技学院动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室动物健康互联网检测技术浙江省工程实验室动物医学与健康管理浙江省国际科技合作基地中澳动物健康大数据分析联合实验室浙江杭州311300

为了解江浙皖地区鸡传染性鼻炎流行状况,对浙江、江苏和安徽部分地区疑似鸡传染性鼻炎的8个鸡场进行样品采集,对122份样品进行病原菌分离,对10株分离株进行形态学、卫星试验、PCR鉴定、生化鉴定和血清型鉴定等检测;利用分离株人工感染SP... 详细信息

为了解江浙皖地区鸡传染性鼻炎流行状况,对浙江、江苏和安徽部分地区疑似鸡传染性鼻炎的8个鸡场进行样品采集,对122份样品进行病原菌分离,对10株分离株进行形态学、卫星试验、PCR鉴定、生化鉴定和血清型鉴定等检测;利用分离株人工感染SPF鸡的动物回归试验,对攻毒后7 d和14 d的鸡只进行病理剖检和病理组织切片观察。结果表明,122份样品中副鸡禽杆菌阳性数为48个,阳性率为39.3%,8个养殖场的阳性率为36.8%~50.0%。10株副鸡禽杆菌分离菌,其中A型3株、B型4株、C型3株,致病率为60%~80%。表明不同血清型副鸡禽杆菌在江浙皖地区均有流行,具有一定的致病性,这为鸡传染性鼻炎三价疫苗研发和防控提供参考。

关键词：鸡传染性鼻炎副鸡禽杆菌分离鉴定

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Clonal integration benefits invasive alien plants under water variability in a native community

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Journal of Plant Ecology 2019年第3期12卷 574-582页

作者： Duo Chen Hao Xiong Chang-Gen Lin Wei He Zhuo-Wen zhang Hui Wang Yong-jian Wang Hubei Engineering technology Research Center for Forestry Information College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China

Aims Many invasive alien plant species are clonal and can greatly propa-gate and spread through clonal integration(sharing resources between connected ramets)in heterogeneous and variable ***,we tested whether water variability influences clonal integration of invasive alien plant species and consequently facili-tates their growth and dominance in a native *** We selected four typical invasive clonal plant species in ***(with clonal integration)and disconnected(without clonal integration)clonal fragments were established either under constant watering or variable watering condition in an experimental native plant community consisting of three naturally co-occurring grassland *** part of the container received high nu-trient and distal part received low *** Findings Clonal integration significantly increased biomass,aboveground mass and belowground mass of invasive alien plants in the proximal ramets,the distal ramets and the whole clone and de-creased the growth of native ***,clonal in-tegration significantly increased the growth of invasive plants in variable *** positive effect of clonal integration was stronger in variable watering than in constant *** plants with clonal integration had high biomass proportion(>0.6)in the whole *** results suggest that invasive clonal plants benefit more from clonal integration in variable water en-vironments when established in a native community,and to some extent,clonal integration potentially contribute greatly to the inva-siveness of alien clonal plants when they enter a new community with resource variability.

关键词： biomass proportion nutrient heterogeneity physiological integration plant invasion resource variability

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高熵合金纳米电催化剂的合成

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稀有金属 2024年第1期48卷 90-104页

作者：薛天雨黄仲谷昊辉张海军武汉科技大学材料学部省部共建耐火材料与冶金国家重点实验室湖北武汉430081

相较于单金属和双金属催化剂,高熵合金(HEAs)催化剂因具有多种活性位点而表现出优异的协同效应和催化活性,当其粒径细化至纳米尺度时,纳米尺寸效应与多元活性位点赋予了高熵合金纳米颗粒(np-HEAs)催化剂较低的过电位,近年来在电化学领... 详细信息

相较于单金属和双金属催化剂,高熵合金(HEAs)催化剂因具有多种活性位点而表现出优异的协同效应和催化活性,当其粒径细化至纳米尺度时,纳米尺寸效应与多元活性位点赋予了高熵合金纳米颗粒(np-HEAs)催化剂较低的过电位,近年来在电化学领域逐渐成为研究热点。目前,np-HEAs催化剂的合成方法有脱合金法、热冲击法、低温液相共还原法、机械合金法、激光烧蚀法及溅射沉积法等。综述了近年来np-HEAs催化剂合成的研究现状,总结了提高其催化活性的策略及措施,并展望了np-HEAs催化剂的未来发展方向。

关键词：高熵合金(HEAs) 高熵合金纳米颗粒(np-HEAs) 催化剂纳米尺寸效应

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ADSORPTION OF TiCl_4 AND ELECTRON DONOR ON DEFECTIVE MgCl_2 SURFACES AND PROPYLENE POLYMERIZATION OVER ZIEGLER-NATTA CATALYST: A DFT STUDY

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Chinese Journal of Polymer science 2013年第4期31卷 591-600页

作者： Rui-hua Cheng Jun Luo Zhen Liu Jing-wen Sun Wei-huan Huang Ming-ge zhang jian-jun Yi 刘柏平 State Key Laboratory of Chemical Engineering East China University of Science and Technology Petrochemical Research Institute China National Petroleum Corporation

The formations of defective MgC12 surfaces, and subsequent adsorption of Ti species and electron donor, as well as propylene polymerization over the Ziegler-Natta catalyst have been investigated using density functional theory （DFT） method. Twelve possible support models of regular and defective MgC12 （110） and （100） surfaces were built. The individual adsorptions of titanium chlorides as mononuclear or dinuclear, and ethyl benzoate （EB） as electron donor, on these models were evaluated. The analysis of energies presented the cases of EB adsorption were generally more stable than titanium chlorides on both surfaces. Thus, EB as internal electron donor mainly prevented TIC14 from coordinating on the MgC12 surfaces where mostly non-stereospecific active sites could be formed. Exceptionally, A5 the site model with terminal Cl-vacancy on the MgC12 support, presented stronger adsorption of TiCl4 than that of EB on （110） surface. Since the TIC14 and ethyl benzoate （EB） would compete to adsorb on the support surface, it seems reasonable to assume that TIC14 might predominately occupy this site, which can act as the most plausible active site for propylene polymerization. The first insertion of propylene monomer into the A5 active site model showed that it exhibited good regioselectivity but poor stereospecificity in the absence of electron donor.

关键词： Supported Ziegler-Natta catalyst Propylene polymerization Defective MgC12 surfaces Electron donor Densityfunctional theory.

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Softening and recrystallization behavior of a new class of ferritic steel

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Journal of Iron and Steel Research International 2019年第2期26卷 154-161页

作者： Xiang-yu Xu jian-zhe Li Xue-min Wang Wei zhang Qian-nan Liu Cheng-jia Shang R.D.K.Misra Collaborative Innovation Center of Steel technology University of Science and Technology BeijingBeijing 100083China school of Materials science and Engineering University of Science and Technology BeijingBeijing 100083China Laboratory for Excellence in Advanced Steel Research Materials Science and Engineering ProgramDepartment of MetallurgicalMaterials and Biomedical EngineeringUniversity of Texas at El PasoEl PasoTX 79968USA

In order to clarify the recrystallization mechanism of low-densityδ-ferrite steel Fe-4AI-2Ni,interrupted and single-pass compression tests were carried *** this regard,five deformation temperatures(750-950 at an interval of 50°C)and different hold time were *** was observed that the softening and recrystallization fraction was enhaneed with increased deformation temperature and hold *** original grain bounclaries were the preferred nucleation sites for recrystallized grains,and recrystallization had an impact on obtaining homogeneous and fine-grained *** in the ferritic alloy commenced after a significant degree of softening,and the softening associated with recovery was appreciably *** optimum rolling deformation temperature was identified to be greater than 900°C.

关键词： δ-ferrite steel Interrupted compression test Recovery Recrystallization

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Chinese Physics B 2018年第8期27卷 386-392页

作者： Xiao-Yu Han Jun-Hong Wang＊ Mei-E Chen Zhan zhang Zheng Li Yu-jian Li Institute of Lightwave technology Beijing Jiaotong UniversityBeijing 100044China

In order to validate the similarity principle of microwave breakdown, a two-dimensional （2D） fluid model of low- pressure microwave argon plasma is established and solved by the finite-element method. Proportional conditions are used in this model to build three different breakdown processes that meet the premise of a similarity principle, and these breakdown processes are called ＂similar cases＂ in this paper. Similar cases have proportionately sized breakdown regions, where the ratio of frequency of incident microwave f to gas pressure p （f/p）, and the reduced field E/p in them are kept the same. All the important physical parameters such as electron density, electron temperature, and reduced electric field can be obtained from the simulation of this model. The results show that the parameters between similar cases are in constant ratio without changing with time, which means that the similarity principle is also valid in microwave breakdown.

关键词： similarity principle microwave plasma low pressure 2D fluid model

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