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Decabromodiphenyl ether induces the chromosome association disorders of spermatocytes and deformation failures of spermatids in mice

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Journal of Environmental Sciences 2024年第4期138卷 531-542页

作者： Jinglong Xue Xiangyang Li Yafei Chi Leqiang Gao Yue Zhang Yan Wang Moxuan Zhao jialiu wei Zhixiong Shi Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology Capital Medical UniversityBeijing 100069China Laboratory Animal Center Capital Medical UniversityBeijing 100069China Key Laboratory of Cardiovascular Epidemiology&Department of Epidemiology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

The environmental presence of decabromodiphenyl ether(BDE-209),which is toxic to the male reproductive system,is *** current study investigated its mechanism of toxicity in *** results showed,that BDE-209 induced DNA damage,decreased the expression of the promoter of meiosis spermatogenesis-and oogenesis-specific basic helix-loop-helix 1(Sohlh1),meiosis related-factors Lethal(3)malignant brain tumor like 2(L3MBTL2),PIWI-like protein 2(MILI),Cyclin-dependent kinase 2(CDK2),Cyclin A,synaptonemal complex protein 1(SYCP1)and synaptonemal complex protein 3(SYCP3),and caused spermatogenic cell apoptosis,resulting in a decrease in sperm quantity and ***,BDE-209 downregulated the levels of anaphase-promoting complex/cyclosome(APC/C),increased the expression of PIWI-like protein 1(MIWI)in the cytoplasm of elongating spermatids,and decreased the nuclear levels of RING finger protein 8(RNF8),ubiquitinated(ub)-H2A/ub-H2B,and Protamine 1(PRM1)/Protamine 2(PRM2),while increasing H2A/H2B nuclear levels in *** reproductive toxicity was persistent for 50 days following the withdrawal of BDE-209 *** results suggested that BDE-209 inhibits the initiation of meiosis by decreasing the expression of ***,the reduced expression of L3MBTL2 inhibited the formation of chromosomal synaptonemal complexes by depressing the expression of meiosis regulators affecting the meiotic progression and also inhibited histone ubiquitination preventing the replacement of histones by protamines,by preventing RNF8 from entering nuclei,which affected the evolution of spermatids into mature sperm.

关键词： BDE-209 Meiosis L3MBTL2 Sperm RNF8 Histone ubiquitination

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Silica nanoparticles induce abnormal mitosis and apoptosis by balance and regulation of PKC mediated positive and negative signaling pathways

Silica nanoparticles induce abnormal mitosis and apoptosis b...

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第六届中国毒理学会免疫毒理专业委员会换届及学术会议

作者： Jin Zhang Lihua Ren Lianshuang Zhang jialiu wei Liu jianhui Zhiwei Sun Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Researches had shown mat SiNPs could damage seminiferous epithelium and reduce the quantity and quality of sperms,but little is known about the toxicity mechanism of SiNPs on male reproductive *** investigate toxic mechanism of SiNPs,male germ cell lines(GC-2spd) were exposed to 0,6.25,12.5,25 or 50μg/mL SiNPs for 24 h,*** results showed that SiNPs resulted in a dose-dependent reduction in cell viability,as well as increases in the LDH release and cell apoptosis *** can lead to DNA strand breaks and abnormal *** addition,SiNPs induced cell cycle arrest in the GO/Gl phase and proliferation *** could simultaneously activate negative signaling pathway(PKC-5/p53/p21) by PKC-5 and positive signaling pathway(PKC-α/MAPK) by PKC-α.However the lower expressions of cyclin E and CDK2 indicated that negative signaling pathway mediated by PKC-8 played a dominant role in the process of cell *** results suggested that SiNPs might activate PKC-8 mediated negative signaling due to DNA strand breaks resulting from *** activity PKC-8mediated negative signaling arrested GC-2 cells at GO/Gl phase,resulting in abnormal mitosis and cell apoptosis.

关键词： Silica nanoparticles mitosis GC-2spd cells Cell cycle PKC

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Silica nanoparticles exposure inducing reproductive toxicity by DNA damage and mitochondrial apoptosis pathway in female Balb/c mice

Silica nanoparticles exposure inducing reproductive toxicity...

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中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Jianhui Liu Man Yang Li Jing Lihua Ren jialiu wei Jin Zhang Xianqing Zhou Zhiwei Sun Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Background:Given that silica nanoparticles(SiNPs) has raised concerns about their potential risks to human health,this study is aimed to explore the influence of SiNPs on reproductive function and its mechanism in female ***:In this study,80 female mice were randomly divided into four groups:a control group,three SiNPs groups(7,21,35 mg/kg SiNPs).Mice in the SiNPs group were treated with SiNPs at different dose by tracheal perfusion every 3 days for 15 days(in total 5 times),mice in the control group were given the same volume of physiological *** each group,a half number of the mice were sacrificed after anesthesia on the 15th day,and the remaining half were sacrificed on the 30 th ***:SiNPs increased the number of atresia follicles on the 15th day after the first *** Ultrastructural results showed that mitochondria were damage on ovary cells of *** induced oxidative stress and DNA damage in *** balance of hormone levels was disturb after SiNPs exposure on the 15th ***,expressions of ATM/CHK-2/E2F1/P73/P53/BAX/Caspase-9/Caspase-3 were greatly up-regulated and expressions of RAD51 were down-regulated after SiNPs ***,compared with the control group,the number of atresia follicles,hormone levels,DNA damage and the expression of ATM/CHK-2/E2F1/P73/P53/BAX/Caspase-9/Caspase-3 showed no significant changes in the SiNPs group on the 30 th day,but the expression of RAD51 was increased in SiNPs ***:In summary,the results demonstrate that SiNPs induce atresia may be associated with apoptosis and hormone level *** induce apoptosis in ovary cells by activating the mitochondrial apoptosis pathway,whereas DNA damage and DNA repair failure may be connected with *** addition,damage to the female reproductive system caused by SiNPs may be reversible.

关键词： apoptosis Silica nanoparticles exposure inducing reproductive toxicity by DNA damage and mitochondrial apoptosis pathway in female Balb/c mice DNA

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Fine particulate matters inducing reproductive impairment by causing cell cycle arrest and activating mitochondrial apoptosis pathway in male rats and spermatocyte cells

Fine particulate matters inducing reproductive impairment by...

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中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Jianhui Liu Lihua Ren jialiu wei Jin Zhang Yannan Zhang Hejing Hu Junchao Duan Zhiwei Sun Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Fine particulate matters(PM2.5) have been shown to induce male reproductive toxicity,but the mechanism is *** explore the toxic mechanism of PM2.5,32 male Sprague-Dawley(SD)rats were treated to normal saline(control group) or PM2.5 with the doses of 1.8,5.4 and 16.2 mg/*** intratracheal instillation every 3 days for 30 *** 30 days,the rats were sacrificed for tissue *** vitro,GC-2 spd cells were treated to various concentrations(12.5,25,50 μg/mL) of PM2.5 for 24 *** results showed that PM2.5 obviously decreased sperm quality,increased sperm abnormality rate and damaged testicular tissue in male ***2.5 induced oxidative stress and DNA damage in ***,PM2.5 significantly increased the expression of BAX,cytochrome C,Caspase-9,Caspase-3 on the 30 th *** vitro,GC-2 cells treated with PM2.5 displayed mitochondria swelling and cristae ***2.5 induced S-phase cell cycle arrest and apoptosis in GC-2 spd *** results suggest that PM2.5 might induce apoptosis by up-regulating the expressions of mitochondrial apoptosis pathway factors through causing DNA damage and cell cycle arrest resulting from oxidative stress,and cause a decrease in the quantity and quality of sperms.

关键词： Fine particulate matters oxidative stress cell cycle arrest mitochondrial apoptosis pathway male reproduction

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Silica nanoparticles exposure inducing reproductive toxicity by DNA damage and mitochondrial apoptosis pathway in female Balb/c mice

Silica nanoparticles exposure inducing reproductive toxicity...

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第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会

作者： Jianhui Liu Man Yang Li Jing Lihua Ren jialiu wei Jin Zhang Feng Zhang Junchao Duan Xianqing Zhou Zhiwei Sun Department of Toxicology and Hygienic Chemistry Capital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University College of life science Qilu normal Normal University

Given that the effects of ultrafine fractions(<0.1μm)on reproductive diseases are gaining attention,this study aimed to explore the influence of silica nanoparticles(SiN Ps)-induced female reproductive *** this study,80 female mice were randomly divided into four groups including a control group and three concentrations of SiNPs

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土钉支护的离心模型试验研究

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土木工程学报 2009年第1期42卷 76-80页

作者：张建红张雁濮家骝韦忠欣高印立清华大学水沙科学与水利水电工程国家重点实验室北京100084 中国建筑科学研究院北京100013

土钉由于其施工简便、经济高效,已做为原位土体加固和支护技术被广泛应用。但是对于土钉加固机理的了解还有待于深入研究。中国建筑科学研究院地基所和清华大学共同完成了土钉支护机理的离心模型试验研究。采用清华大学中型土工离心机... 详细信息

土钉由于其施工简便、经济高效,已做为原位土体加固和支护技术被广泛应用。但是对于土钉加固机理的了解还有待于深入研究。中国建筑科学研究院地基所和清华大学共同完成了土钉支护机理的离心模型试验研究。采用清华大学中型土工离心机进行了8组土钉支护基坑的离心模型试验。离心模型试验研究表明,土钉能够显著提高基坑土体的稳定性。土钉长度和密度对支护基坑土体的稳定性影响非常显著。在本文所研究的土性和基坑条件下,当土钉最大长度和基坑高度之比(L/H)为0.48和0.8时,基坑土体基本不会发生整体剪切破坏。土钉支护土体发生外部整体剪切破坏的条件是高密度布置短钉情况,土钉支护土体发生内部钉筋拔出破坏的条件是稀疏布置长钉情况。

关键词：土钉支护离心模型试验

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The Puccinia striiformis effector Hasp98 facilitates pathogenicity by blocking the kinase activity of wheat TaMAPK4

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Journal of Integrative Plant Biology 2023年第1期65卷 249-264页

作者： Jinping wei Xiaodong Wang Zeyu Hu Xiaojie Wang jialiu Wang Jianfeng Wang Xueling Huang Zhensheng Kang Chunlei Tang State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University Yangling 712100 China Pioneering Innovation Center for Wheat Stress Tolerance Improvement State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F UniversityYangling 712100 China State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F University Yangling 712100 China

The obligate biotrophic fungus Puccinia striiformis ***(Pst)employs virulence effectors to disturb host immunity and causes devastating stripe rust ***,our understanding of how Pst effectors regulate host defense responses remains *** this study,we determined that the Pst effector Hasp98,which is highly expressed in Pst haustoria,inhibits plant immune responses triggered by flg22or nonpathogenic *** of Hasp98 in wheat(Triticum aestivum)suppressed avirulent Pst-triggered immunity,leading to decreased H2O2accumulation and promoting *** infection,whereas stable silencing of Hasp98 impaired *** ***98 interacts with the wheat mitogenactivated protein kinase TaMAPK4,a positive regulator of plant resistance to stripe *** conserved TEY motif of TaMAPK4 is important for its kinase activity,which is required for the resistance *** demonstrate that Hasp98inhibits the kinase activity of TaMAPK4 and that the stable silencing of TaMAPK4 compromises wheat resistance against *** results suggest that Hasp98 acts as a virulence effector to interfere with the MAPK signaling pathway in wheat,thereby promoting *** infection.

关键词： effector MAPK plant immunity Puccinia striiformis f.sp.tritici

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耐磨铝基超疏水材料的制备及其动态冷凝行为

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材料研究学报 2020年第4期34卷 277-284页

作者：王芳周宝玉冯伟雷家柳姜玉凤王琦迪湖北理工学院材料科学与工程学院黄石435003 湖北理工学院矿区环境污染控制与修复湖北省重点实验室黄石435003 昆明理工大学冶金与能源工程学院昆明650000

用水热法制备了铝基超疏水材料。SEM观测结果表明,这种材料表面有明显的微纳米复合结构;用动态摩擦试验机进行了循环摩擦实验,结果表明:这种材料的耐磨性能优异,2000次循环摩擦后样品表面变得略微平整,微米结构丢失,但是保留有大量ZnO... 详细信息

用水热法制备了铝基超疏水材料。SEM观测结果表明,这种材料表面有明显的微纳米复合结构;用动态摩擦试验机进行了循环摩擦实验,结果表明:这种材料的耐磨性能优异,2000次循环摩擦后样品表面变得略微平整,微米结构丢失,但是保留有大量ZnO纳米棒,仍保持超疏水特性。这种材料还具有良好的耐酸碱腐蚀能力。冷凝实验结果表明:冷凝液滴在微纳米复合结构中随机生成,有益的是凹槽中的冷凝液滴在长大和合并过程中逐渐脱离凹槽底部最终悬浮在粗糙结构的表面。这证实,冷凝液滴在样品表面保持Cassie态,为冷凝过程中频繁发生液滴合并自弹跳提供了条件。

关键词：材料表面与界面超疏水材料水热法耐磨性冷凝液滴合并自弹跳

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中国作家协会办公楼公共艺术实践

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装饰 2007年第12期 84-85页

作者：李家骝陈晓林魏二强清华大学美术学院

这次受中国作家协会委托，我们几位教师领着研究生、本科生用近一年时间实现了一次较完整的整体壁画、雕塑设计实践活动。

关键词：公共艺术协会作家中国办公楼实践活动研究生本科生

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外周静脉-动脉血二氧化碳分压差可预测感染性休克患者预后:62例前瞻例研究

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南方医科大学学报 2018年第11期38卷 1312-1317页

作者：高伟张勇倪海滨张家留周丹丹殷丽萍张丰陈浩张蓓蓓李伟南京中医药大学附属中西医结合医院重症医学科江苏南京210028 南京中医药大学附属中西医结合医院神经外科江苏南京210028

目的探讨早期复苏后外周静脉-动脉血二氧化碳分压差对感染性休克患者预后的预测价值。方法采用前瞻性研究方法,选择2017年5月~2018年5月南京中医药大学附属中西医结合医院重症医学科收治的感染性休克患者,测定患者早期复苏6 h后中心静... 详细信息

目的探讨早期复苏后外周静脉-动脉血二氧化碳分压差对感染性休克患者预后的预测价值。方法采用前瞻性研究方法,选择2017年5月~2018年5月南京中医药大学附属中西医结合医院重症医学科收治的感染性休克患者,测定患者早期复苏6 h后中心静脉、动脉及外周静脉血血气分析,记录患者中心静脉、动脉及外周静脉血二氧化碳分压(PCO_2),计算患者外周静脉-动脉血二氧化碳分压差(Ppv-aCO_2)及中心静脉-动脉血二氧化碳分压差(Pcv-aCO_2),根据患者28 d预后将患者分为存活组及死亡组,采用Pearson相关性分析法分析Ppv-aCO_2与Pcv-aCO_2相关性,采用多因素Logistic分析筛选患者死亡的危险因素,并通过受试者工作特征曲线(ROC)评价各项指标预测患者预后的价值。结果共入选62例感染性休克患者,28 d存活35例,死亡27例。与存活组比较,死亡组患者急性生理与慢性健康评分Ⅱ(APACHEⅡ)(24.2±6.0 vs 20.5±4.9,P=0.011)及序贯器官衰竭的评分(SOFA)(14.9±4.7 vs 12.2±4.5,P=0.027)明显升高。6 h复苏后死亡组患者Pcv-aCO_2(5.5±1.6 vs 7.1±1.7,P<0.001),Ppv-aCO_2(7.1±1.8 vs 10.0±2.7,P<0.001),及动脉乳酸(Lac)(3.3±1.2 vs 4.2±1.3,P=0.003)明显高于存活组。Pearson相关性分析显示PpvaCO_2与Pcv-aCO_2明显相关,r=0.897,R^2=0.805,P<0.001。多因素Logistic回归分析显示Ppv-aCO_2和Lac是感染性休克患者28 d生存率的独立预后因素[(Ppv-aCO_2:β=0.625,P=0.001,相对危险度(OR)=1.869,95%CI:1.311~2.664;Lac:β=0.584,P=0.041,OR=1.794,95%CI:1.024~3.415)]。ROC曲线分析显示,Ppv-aCO_2、Pcv-aCO_2和Lac对感染性休克患者预后均有预测价值,其中Ppv-aCO_2的ROC曲线下面积(AUC)最大,为0.814(95%CI:0.696~0.931,P<0.001),最佳临界值为9.05 mmHg时,预测患者28 d死亡的敏感度为70.4%,特异度为88.6%;Lac的AUC=0.732(95%CI:0.607~0.858,P=0.002),最佳临界值为3.45 mmol/L时,敏感度为70.4%,特异度为74.3%;Pcv-aCO_2的AUC=0.766(95%CI:0.642~0.891,P<0.001),最佳临界值为7.05 mmHg时,敏感度为66.7%,特异度为80.0%。结论Ppv-aCO_2与Pcv-aCO_2相关,与感染性休克患者预后相关,可作为评估感染性休克患者28 d生存率的独立预后指标。

关键词：外周静脉-动脉血二氧化碳分压差中心静脉-动脉血二氧化碳分压差感染性休克预后

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