检索结果-南通市图书馆

Actively priming autophagic cell death with novel transferrin receptor-targeted nanomedicine for synergistic chemotherapy against breast cancer

引用

Acta Pharmaceutica Sinica B 2019年第5期9卷 1061-1077页

作者： Dong Mei Binlong Chen Bing He Haibin Liu Zhiqiang lin jialiang lin Xiaoyan Zhang Ning Sun Libo Zhao Xiaoling Wang Qiang Zhang Clinical Research Center Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijing100045China Key Laboratory of Molecular Pharmaceutics New Drug Delivery SystemsSchool of Pharmaceutical SciencesPeking UniversityBeijing100191China Department of General Surgery China-Japan Friendship HospitalBeijing100029China Institute of Systems Biomedicine School of Basic Medical SciencesPeking University Health Science CenterBeijing100191China

Recently, considerable attention in the field of cancer therapy has been focused on the mammalian rapamycin target(m TOR), inhibition of which could result in autophagic cell death(ACD). Though novel combination chemotherapy of autophagy inducers with chemotherapeutic agents is extensively investigated, nanomedicine-based combination therapy for ACD remains in infancy. In attempt to actively trigger ACD for synergistic chemotherapy, here we incorporated autophagy inducer rapamycin(RAP) into 7 pep-modified PEG-DSPE polymer micelles(7 pep-M-RAP) to specifically target and efficiently priming ACD of MCF-7 human breast cancer cells with high expression of transferrin receptor(Tf R). Cytotoxic paclitaxel(PTX)-loaded micelle(7 pep-M-PTX) was regarded as chemotherapeutic drug model. We discovered that with superior intracellular uptake in vitro and more tumor accumulation of micelles in vivo, 7 pep-M-RAP exhibited excellent autophagy induction and synergistic antitumor efficacy with 7 pep-M-PTX. Mechanism study further revealed that 7 pep-M-RAP and 7 pep-MPTX used in combination provided enhanced efficacy through induction of both apoptosis-and mitochondria-associated autophagic cell death. Together, our findings suggested that the targeted excess autophagy may provide a rational strategy to improve therapeutic outcome of breast cancer, and simultaneous induction of ACD and apoptosis may be a promising anticancer modality.

关键词： Autophagic cell death Combination therapy Targeted delivery Rapamycin Breast cancer Transferrin receptor Mitophagy Nanomedicines 7pep

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A magnetism/laser-auxiliary cascaded drug delivery to pulmonary carcinoma

引用

Acta Pharmaceutica Sinica B 2020年第8期10卷 1549-1562页

作者： jialiang lin Qingqing Yin Binlong Chen Haoran Zhang Dong Mei Jijun Fu Bing He Hua Zhang Wenbing Dai Xueqing Wang Yiguang Wang Qiang Zhang Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems School of Pharmaceutical SciencesPeking UniversityBeijing 100191China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China School of Pharmaceutical Science Guangzhou Medical UniversityGuangzhou 511436China

Although high-efficiency targeted delivery is investigated for years, the efficiency of tumor targeting seems still a hard core to smash. To overcome this problem, we design a three-step delivery strategy based on streptavidin-biotin interaction with the help of c(RGDfK), magnetic fields and lasers.The ultrasmall superparamagnetic iron oxide nanoparticles(USIONPs) modified with c(RGDfK) and biotin are delivered at step 1, followed by streptavidin and the doxorubicin(Dox) loaded nanosystems conjugated with biotin at steps 2 and 3, respectively. The delivery systems were proved to be efficient on A549 cells. The co-localization of signal for each step revealed the targeting mechanism. The external magnetic field could further amplify the endocytosis of USPIONs based on c(RGDfK), and magnify the uptake distinctions among different test groups. Based on photoacoustic imaging, laser-heating treatment could enhance the permeability of tumor venous blood vessels and change the insufficient blood flow in cancer. Then, it was noticed in vivo that only three-step delivery with laser-heating and magnetic fields realized the highest tumor distribution of nanosystem. Finally, the magnetism/laser-auxiliary cascaded delivery exhibited the best antitumor efficacy. Generally, this study demonstrated the necessity of combining physical, biological and chemical means of targeting.

关键词： auxiliary overcome delivery

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

贵州省典型铅锌矿区和贵阳市居民汞暴露以及健康风险评估

引用

生态学杂志 2024年第3期43卷 869-877页

作者：王达卫孔林徐晓航韩佳良刘杰民仇广乐中国科学院地球化学研究所环境地球化学国家重点实验室贵阳550081 中国科学院大学北京100049 贵州医科大学公共卫生学院环境污染与疾病监控教育部重点实验室贵阳550025 贵州省人民医院贵阳550025

选择贵州省赫章县铅锌矿区和贵阳市居民区,采集了居民日用食物(大米、土豆、鸡蛋、蔬菜、鱼肉、猪肉)、饮用水和农作物根际土壤,共计503个,测定了总汞和甲基汞的含量,并结合蒙特卡洛模拟评估了居民无机汞和甲基汞暴露风险。结果如下:(1... 详细信息

选择贵州省赫章县铅锌矿区和贵阳市居民区,采集了居民日用食物(大米、土豆、鸡蛋、蔬菜、鱼肉、猪肉)、饮用水和农作物根际土壤,共计503个,测定了总汞和甲基汞的含量,并结合蒙特卡洛模拟评估了居民无机汞和甲基汞暴露风险。结果如下:(1)铅锌矿区的样品总汞和甲基汞的范围分别为0.11~39和0.028~1.7 ng·g^(-1),城市居民区的样品总汞和甲基汞的范围分别为0.07~18和0.01~7.7 ng·g^(-1),除蔬菜外的样品总汞含量低于我国食品限量标准,蔬菜总汞含量分别有14.7%(铅锌矿区)和17.7%(城市居民区)的样品超过我国《食品安全国家标准》(GB 2672—2017)规定的10 ng·g^(-1)。(2)两个地区无机汞日暴露量无差异(城市居民区:0.031μg·kg^(-1)·d-1,铅锌矿区:0.032μg·kg^(-1)·d-1),但城市居民区的甲基汞日暴露量(0.006μg·kg^(-1)·d-1)高出铅锌矿区(0.0032μg·kg^(-1)·d-1)近1倍。(3)两个地区居民无机汞和甲基汞的平均日暴露量分别低于联合国粮农组织/世界卫生组织食品添加剂联合专家委员会(JECFA)推荐的人体安全无机汞的临时容许每周摄入量(PTWI)4μg·kg^(-1)·week-1和美国环境保护署(USEPA)推荐的甲基汞参考剂量(RfD)0.1μg·kg^(-1)·d-1,暴露风险指数均小于1,表明处于健康安全水平。

关键词：铅锌矿区贵阳市居民区无机汞甲基汞风险评估

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preliminary study on the safety and efficacy of a new polymyxin B-immoblized resin column in treatment of LPS-induced sepsis beagles

引用

Animal Models and Experimental Medicine 2022年第2期5卷 161-171页

作者： Yonggui Li Zhenggen Yang jialiang Hu Zhennan lin Research and Development Center Guangzhou Koncen Bioscience Co.Ltd.GuangzhouPR China Guangdong Provincial Key Laboratory of Hemoadsorption Technology GuangzhouPR China

Background:This study aims to assess the safety and efficacy of direct hemoperfu-sion using a new polymyxin B-immobilized resin column(disposable endotoxin ad-sorber,KCEA)in an endotoxin/lipopolysaccharide(LPS)-induced sepsis model.Methods:Eighteen beagles were randomized into 1 intervention group(KCEA group,n=6)and 2 control groups(sham group and model group,n=6 each).Sepsis was in-duced by continuous intravenous application of 0.5 mg/kg body weight of endotoxin for 60 min.An extracorporeal hemoperfusion device made with KCEA for endotoxin adsorption was used.Model group beagles received standard treatment with fluids and vasoactive drugs,KCEA group beagles received standard treatment and direct hemoperfusion of KCEA for 2 h,and sham group beagles were treated with standard treatment and direct hemoperfusion of a sham column for 2 h.Results:Good blood compatibility of KCEA was confirmed by assessing clinical pa-rameters.Blood endotoxin peak levels in the KCEA group were significantly lower,resulting in a significant suppression of IL-6,TNF-αand procalcitonin,which improved mean arterial pressure and significantly lowered vasopressor demand,thereby pro-tecting organ function and improving survival time and rate.In the KCEA group,MAP was significantly higher over 6 h than those recorded both in the sham group and model group.The 7-day survival rates of the KCEA,sham and model groups were 50%,0%and 0%,respectively.Conclusion:KCEA hemoadsorption was effective at detoxifying circulatory endotoxin and inflammatory mediators and contributed to the decreased mortality rate in the sepsis beagles.

关键词： endotoxin hemoadsorption polymyxin B sulfate resin sepsis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

数字化新时代银政融合助力乡村振兴的积极探索

引用

金融科技时代 2024年第5期32卷 33-39页

作者：何欣方宇擎林嘉良交通银行广东省分行

近年来,商业银行助力乡村振兴力度不断加大,支持乡村振兴的金融服务逐渐多元化。然而,随着乡村振兴的全面推进,乡村产权制度不完善、信用体系及金融组织体系不健全等矛盾日益突出。文章聚焦数字化新时代背景下,商业银行通过推进银政融合... 详细信息

近年来,商业银行助力乡村振兴力度不断加大,支持乡村振兴的金融服务逐渐多元化。然而,随着乡村振兴的全面推进,乡村产权制度不完善、信用体系及金融组织体系不健全等矛盾日益突出。文章聚焦数字化新时代背景下,商业银行通过推进银政融合,从时间和空间两个维度助力乡村振兴的模式探索,并以交通银行广东省分行实践为例,分享了商业银行通过金融科技和银政融合服务乡村振兴的相关经验和成效。

关键词：乡村振兴银政融合数字乡村

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prediction of network public opinion based on bald eagle algorithm optimized radial basis function neural network

引用

International Journal of Intelligent Computing and Cybernetics 2022年第2期15卷 184-200页

作者： jialiang Xie Shanli Zhang ling lin College of Science Jimei UniversityXiamenChina

Purpose-In the new era of highly developed Internet information,the prediction of the development trend of network public opinion has a very important reference significance for monitoring and control of public opinion by relevant government departments.Design/methodology/approach-Aiming at the complex and nonlinear characteristics of the network public opinion,considering the accuracy and stability of the applicable model,a network public opinion prediction model based on the bald eagle algorithm optimized radial basis function neural network(BES-RBF)is proposed.Empirical research is conducted with Baidu indexes such as“COVID-19”,“Winter Olympic Games”,“The 100th Anniversary of the Founding of the Party”and“Aerospace”as samples of network public opinion.Findings-The experimental results show that the model proposed in this paper can better describe the development trend of different network public opinion information,has good stability in predictive performance and can provide a good decision-making reference for government public opinion control departments.Originality/value-A method for optimizing the central value,weight,width and other parameters of the radial basis function neural network with the bald eagle algorithm is given,and it is applied to network public opinion trend prediction.The example verifies that the prediction algorithm has higher accuracy and better stability.

关键词： Network public opinion COVID-19 Bald eagle algorithm RBF neural network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

骨肉瘤患者癌组织中AHA1 mRNA和LOXL2 mRNA表达与侵袭转移基因mRNA表达的相关性及临床意义

引用

现代检验医学杂志 2024年第2期39卷 39-45页

作者：沈家亮王琳尚文强华北医疗健康集团峰峰总医院骨科河北邯郸056201

目的研究骨肉瘤(osteosarcoma)患者癌组织中热休克蛋白90 ATP酶激活因子1(the activator of HSP90 ATPase-1,AHA1)和赖氨酰氧化酶样蛋白2(lysyl oxidase like-2 protein,LOXL2)表达与侵袭转移基因mRNA表达的相关性及临床意义。方法选取2016年2月~2017年3月华北医疗健康集团峰峰总医院诊治的90例骨肉瘤患者为研究对象。应用实时荧光定量PCR检测组织中AHA1 mRNA,LOXL2 mRNA及侵袭转移基因Wnt家族成员9A(Wnt9a)mRNA,锌指E盒结合同源盒1(Zinc finger E-box binding homeobox 1,ZEB1)mRNA,锌指E盒结合同源盒2(ZEB2)mRNA,N-钙黏素(N-cad)mRNA和波形蛋白(Vim)mRNA表达。采用Pearson相关分析,比较不同临床特征骨肉瘤患者AHA1 mRNA,LOXL2 mRNA表达差异。Kaplan-Meier生存分析影响AHA1 mRNA,LOXL2 mRNA表达对骨肉瘤患者预后的影响。单因素及多因素COX回归分析影响骨肉瘤患者预后的因素。结果骨肉瘤组织中AHA1 mRNA(3.16±0.59),LOXL2 mRNA(2.84±0.44)及侵袭转移基因Wnt9a mRNA(3.23±0.42),ZEB1 mRNA(2.73±0.39),ZEB2 mRNA(2.52±0.56),N-cad mRNA(2.71±0.65),Vim mRNA(2.81±0.73)表达均高于癌旁组织(1.10±0.21,0.95±0.18,0.79±0.15,0.64±0.11,0.98±0.19,0.68±0.14,0.72±0.15),差异具有统计学意义(t=31.206,37.716,51.903,48.931,24.706,28.964,26.605,均P<0.05)。骨肉瘤组织中AHA1 mRNA与LOXL2 mRNA表达呈显著正相关(r=0.712,P<0.05)。骨肉瘤组织中AHA1 mRNA,LOXL2 mRNA与侵袭转移基因Wnt9a mRNA,ZEB1 mRNA,ZEB2 mRNA,N-cad mRNA,Vim mRNA表达呈显著正相关(r=0.504~0.720,均P<0.05)。Eneeking分期Ⅲ期、有软组织浸润和有肺转移骨肉瘤组织中AHA1 mRNA,LOXL2 mRNA表达高于Eneeking分期Ⅰ~Ⅱ期、无软组织浸润和无肺转移患者,差异具有统计学意义(t=14.122~171.054,均P<0.05)。AHA1 mRNA高表达组和低表达组患者五年生存率分别为36.36%(16/44),78.26%(36/46)。AHA1 mRNA高表达组患者五年累积生存率明显低于低表达组,差异有统计学意义(Log-rankχ^(2)=16.081,P<0.05)。LOXL2 mRNA高表达组和低表达组患者五年生存率分别为34.88%(15/43),78.72%(37/47)。LOXL2 mRNA高表达组患者五年累积生存率明显低于低表达组,差异有统计学意义(Log-rankχ^(2)=15.880,P<0.05)。肺转移(OR=1.921,P<0.05),Eneeking分期Ⅲ期(OR=1.906,P<0.05),AHA1 mRNA高表达(OR=1.405,P<0.05),LOXL2 mRNA高表达(OR=1.733,P<0.05)是影响骨肉瘤患者不良生存预后的独立危险因素。结论骨肉瘤组织中AHA1 mRNA,LOXL2 mRNA表达升高,两者表达与侵袭转移基因表达有关,是影响骨肉瘤患者不良生存预后的独立危险因素。

关键词：骨肉瘤热休克蛋白90 ATP酶激活因子1 赖氨酰氧化酶样蛋白2 肿瘤侵袭转移

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

汽车冷却液加注液面问题分析研究

引用

汽车电器 2024年第2期 77-79页

作者：丘建陆家良林亮东风柳州汽车有限公司广西柳州545000 玉柴芯蓝新能源动力科技有限公司广西南宁530007

文章主要分析讨论汽车冷却液加注液面不合格问题,同时提出一些工艺优化加注的措施。首先对汽车冷却液加注的原理、功能和结构等方面做介绍,然后结合汽车冷却系统结构来分析加注冷却液问题。汽车冷却液加注液面过程异常,会导致发动机温... 详细信息

文章主要分析讨论汽车冷却液加注液面不合格问题,同时提出一些工艺优化加注的措施。首先对汽车冷却液加注的原理、功能和结构等方面做介绍,然后结合汽车冷却系统结构来分析加注冷却液问题。汽车冷却液加注液面过程异常,会导致发动机温度升高报警,特别是直接与高温气体接触的零件,会因受热膨胀影响正常的配合间隙,导致运动件受阻甚至卡死,大大影响整车的行驶性能。文章在大量试验和生产经验的基础上,对汽车冷却液加注液面不合格问题进行分析和提出优化方案。

关键词：汽车冷却液加注

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ERRATUM TO:Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival

引用

Science China(Life Sciences) 2024年第7期67卷 1548-1548页

作者： jialiang Yang Yuhong Chen Tongdan Zou Bai Xue Fang Yang Xiangzhou Wang Yibo Huo Boyun Yan Yuxia Xu Shiyu He Yi Yin Jing Wang Xiong Zhu lin Zhang Yu Zhou Zhengfu Tai Ping Shuai Man Yu Qian Luo Yilian Cheng Bo Gong Xianjun Zhu Jing Zhang Xinghuai Sun Ying lin Houbin Zhang Zhenglin Yang Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Department of Ophthalmology and Visual Science Eye and Ear Nose Throat(ENT)HospitalFudan UniversityShanghai 200032China School of Optoelectronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 611731China Institute of Chengdu Biology Sichuan Translational Medicine HospitalChinese Academy of SciencesChengdu 610093China Department of Ophthalmology Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences Sichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalChengdu 610072China

Erratum to:SCIENCE CHINA Life Sciences,Volume 66,Issue 2:211-225(2022),https://***/10.1007/s11427-021-2126-2 This paper contains an error in Figure 4C,where the representative images of filipin staining of retinal sections from 6-month-old mice were misused.We provide the correct picture for Figure 4C as follows.The statistical result in Figure 4D was corrected as well.This new Figure 4 does not affect the conclusion of this article.

关键词： Figure critical conclusion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Roles and implications of mRNA N^(6)-methyladenosine in cancer

引用

Cancer Communications 2023年第7期43卷 729-748页

作者： lingxing Zeng Xudong Huang jialiang Zhang Dongxin lin Jian Zheng Department of Experimental Research Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Etiology and Carcinogenesis National Cancer Center/National Clinical Research Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical UniversityNanjingJiangsuP.R.China

RNA N^(6)-methyladenosine modification is the most prevalent internal modification of eukaryotic RNAs and has emerged as a novel field of RNA epigenetics,garnering increased attention.To date,m^(6)A modification has been shown to impact multiple RNA metabolic processes and play a vital role in numerous biological processes.Recent evidence suggests that aberrant m^(6)A modification is a hallmark of cancer,and it plays a critical role in cancer development and progression through multiple mechanisms.Here,we review the biological functions of mRNA m^(6)A modification in various types of cancers,with a particular focus on metabolic reprogramming,programmed cell death and tumor metastasis.Furthermore,we discuss the potential of targetingm^(6)Amodification or its regulatory proteins as a novel approach of cancer therapy and the progress of research on m^(6)A modification in tumor immunity and immunotherapy.Finally,we summarize the development of different m^(6)A detection methods and their advantages and disadvantages.

关键词： cancer therapy immunotherapy m^(6)A methylation N^(6)-methyladenosine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：