检索结果-南通市图书馆

非伤寒沙门菌对喹诺酮类药物的耐药分子机制研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华预防医学杂志 2024年第2期58卷 248-253页

作者：罗嘉俊曾诗寒黄玉兰冯玉连曾方银李小燕南方医科大学第五附属医院检验医学科广州510900

通过回顾性研究分析,探究非伤寒沙门菌(NTS)对喹诺酮类药物的耐药性及耐药机制。以南方医科大学第五附属医院2020年5月至2021年2月临床标本中分离的105株NTS为研究对象,采用VITEK2 Compact全自动鉴定药敏分析系统和血清学实验对菌株进... 详细信息

通过回顾性研究分析,探究非伤寒沙门菌(NTS)对喹诺酮类药物的耐药性及耐药机制。以南方医科大学第五附属医院2020年5月至2021年2月临床标本中分离的105株NTS为研究对象,采用VITEK2 Compact全自动鉴定药敏分析系统和血清学实验对菌株进行鉴定;用琼脂稀释法检测菌株对环丙沙星、左氧氟沙星和萘啶酸的敏感性;对105株NTS进行全基因组测序,采用Abricate等软件分析菌株的耐药相关基因,包括质粒介导的喹诺酮耐药基因(PMQR)和喹诺酮耐药决定区(QRDR);采用SISTR和MLST分析血清型和ST型,并构建系统发育树。结果显示,分离的NTS主要为ST34鼠伤寒沙门菌(53.3%)。药敏结果显示NTS对环丙沙星、左氧氟沙星和萘啶酸的耐药率分别为30.4%、1.9%和22.0%,对环丙沙星、左氧氟沙星的中介率为27.6%、54.2%;62株喹诺酮类非敏感株中共有46株(74.2%)携带PMQR基因,主要为qnrS1(80.4%),其次为aac(6′)-Ib-cr(15.2%);QRDR突变分析共有14株和8株NTS分别存在gyrA和parC基因突变,gyrA均为第87位氨基酸位点突变,分别为Asp87Tyr、Asp87Asn、Asp87Gly,parC检出Thr57Ser突变。综上,本研究发现,NTS对喹诺酮类药物耐药性相对较高,携带qnrS1基因主要导致NTS对环丙沙星和左氧氟沙星的敏感性下降,发生gyrA:87位点突变主要导致NTS对萘啶酸耐药;同时携带多种PMQR和存在QRDR突变,可导致NTS对3种喹诺酮类药物耐药。鼠伤寒沙门菌在临床分离株中存在克隆传播现象,需进一步加强流行病学的监测。

关键词：沙门菌属喹诺酮耐药基因耐药机制

基于膦氧化物钝化的热蒸发像素化钙钛矿发光二极管

在线全文

学校读者我要写书评

暂无评论

发光学报 2024年第1期45卷 1-10页

作者：刘念罗家俊杜培培刘征征杜鹃唐江华中科技大学武汉光电国家研究中心湖北武汉430074 华中科技大学集成电路学院湖北武汉430074 国科大杭州高等研究院物理与光电工程学院浙江杭州310024 中国科学院上海光学精密机械研究所强场激光物理国家重点实验室上海201800

热蒸发法是实现钙钛矿发光二极管商业化显示应用的可靠技术。然而,热蒸发沉积的钙钛矿薄膜的PLQY经常较低,并且钝化手段不如溶液法丰富。本文报道了一种通过原位共蒸技术将钝化剂引入钙钛矿层的方法,这种方法能够钝化真空沉积钙钛矿中... 详细信息

热蒸发法是实现钙钛矿发光二极管商业化显示应用的可靠技术。然而,热蒸发沉积的钙钛矿薄膜的PLQY经常较低,并且钝化手段不如溶液法丰富。本文报道了一种通过原位共蒸技术将钝化剂引入钙钛矿层的方法,这种方法能够钝化真空沉积钙钛矿中的缺陷,增强辐射复合,并提高钙钛矿的PLQY。氧膦基团与不饱和位点形成配位络合,钝化了钙钛矿的晶界缺陷,并抑制了带尾态缺陷。基于最佳比例的钙钛矿薄膜所制备的LED器件表现出最大6.3%的EQE,最大亮度为35642 cd/m^(2)。更进一步地,基于全真空的器件制备工艺,获得了最大EQE为5.0%的312 ppi高分辨率PeLEDs。总之,本文为热蒸发PeLEDs的缺陷钝化提供了有用的指导,证明热蒸发PeLEDs在效率和亮度提升方面具有巨大潜力,并具备商业化前景。

关键词：钙钛矿发光二极管热蒸发缺陷钝化像素化

烟碱转运候选基因NtABCG6L生物信息学及表达特征分析

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

山东农业科学 2024年第4期56卷 20-27,45页

作者：龙本山莫泽君张静瑶彭丽沙段丽丽罗家骏刘仁祥贵州大学烟草学院贵州贵阳550025 贵州省烟草品质研究重点实验室贵州贵阳550025

为预测前期筛选到的可能影响烟草烟碱含量的NtABCG6L基因是否具有转运烟碱功能,本研究采用生物信息学方法对NtABCG6L基因的启动子序列以及编码蛋白的理化性质和结构进行分析,利用软件对烟碱与NtABCG6L蛋白进行分子对接,并分别在烟株打顶... 详细信息

为预测前期筛选到的可能影响烟草烟碱含量的NtABCG6L基因是否具有转运烟碱功能,本研究采用生物信息学方法对NtABCG6L基因的启动子序列以及编码蛋白的理化性质和结构进行分析,利用软件对烟碱与NtABCG6L蛋白进行分子对接,并分别在烟株打顶0、7、14、21 d取根、茎、叶测定烟碱含量和NtABCG6L基因的相对表达量,以分析NtABCG6L基因表达量与烟碱转运效率的关系。结果表明,NtABCG6L基因共编码729个氨基酸;NtABCG6L蛋白分子量为81.29 kDa,理论pI值为8.97,含有ABC_transporter-like_ATP-bd、ABCG_dom、ABC_2_trans结构域,二级结构主要由α-螺旋和无规则卷曲组成,存在跨膜结构,主要定位在细胞膜上,预测NtABCG6L是一个跨膜转运蛋白。NtABCG6L蛋白与烟碱可以通过氢键结合,结合能为-2.95。NtABCG6L基因主要在烟草根部表达,打顶14 d表达量最高,其表达量与烟碱转运系数呈显著正相关关系,初步推测NtABCG6L基因参与了烟碱的转运过程。本研究结果可为进一步采用基因编辑技术明确其功能提供依据。

关键词：烟草 NtABCG6L基因生物信息学分析烟碱转运基因表达

同方期刊数据库博看期刊

The influence of nanotopography on cell behaviour through interactions with the extracellular matrix-A review

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第9期7卷 145-159页

作者： jiajun luo Matthew Walker Yinbo Xiao Hannah Donnelly Matthew J.Dalby Manuel Salmeron-Sanchez Centre for the Cellular Microenvironment University of GlasgowGlasgowG128LTUK

Nanotopography presents an effective physical approach for biomaterial cell manipulation mediated through material-extracellular matrix *** extracellular matrix that exists in the cellular microenvironment is crucial for guiding cell behaviours,such as determination of integrin ligation and interaction with growth *** interactions with the extracellular matrix regulate downstream mechanotransductive pathways,such as rearrangements in the cytoskeleton and activation of signal *** adsorption onto nanotopography strongly influences the conformation and distribution density of extracellular matrix and,therefore,subsequent cell *** this review,we first discuss the interactive mechanisms of protein physical adsorption on ***,we summarise advances in creating nanotopographical features to instruct desired cell ***,we focus on the cellular mechanotransductive pathways initiated by *** review provides an overview of the current state-of-the-art designs of nanotopography aiming to provide better biomedical materials for the future.

关键词： Nanotopography Cell-material interaction Protein adsorption Biomaterials

Predicting microbial extracellular electron transfer activity in paddy soils with soil physicochemical properties using machine learning

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2024年第1期67卷 259-270页

作者： OU jiajun luo XiaoShan LIU JunYang HUANG LinYan ZHOU LiHua YUAN Yong School of Automation Guangdong University of TechnologyGuangzhou 510006China Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangzhou Key Laboratory Environmental Catalysis and Pollution ControlSchool of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China School of Biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China

Soil extracellular electron transfer(EET)is a pivotal biological process within the realm of ***,EET suffers from a lack of predictive ***,an intricately crafted machine learning model has been developed for the purpose of predicting soil EET by using the physicochemical properties of soil as independent input variables and the EET capabilities in terms of current density(j_(max))and Coulombic charge(C_(out))as dependent output *** autoencoder ensemble stacking(AES)model was developed to address the aforementioned issue by integrating support vector machine,multilayer perceptron,extreme gradient boosting,and light gradient boosting machine algorithms as the stacking *** 10-fold crossvalidation,the AES model exhibited notable improvements in predicting j_(max)and C_(out),with average test R^(2)values of 0.83 and 0.84,respectively,surpassing those of single machine learning(ML)models and the basic ensemble *** utilizing partial correlation plots(PDPs),Shapley Additive explanations(SHAP)values,and SHAP decision plots,we quantitatively explained the impact and contribution of the input molecules on the AES model’s predictions of j_(max)and C_(out).In the context of the SHAP method for the AES model,total carbon(TC)was identified as the most correlated descriptor for j_(max),while total organic carbon(TOC)stood out as the most relevant descriptor for C_(out).In the prediction tasks of j_(max)and C_(out)within the AES model,employing a multitask ML approach allowed the model to benefit from the shared information of input variables,thereby enhancing its overall *** study provides a feasible tool for the prediction of soil EET from soil physiochemical properties and an advanced understanding of the relationship between soil physiochemical properties and EET capability.

关键词： extracellular electron transfer paddy soil machine learning prediction autoencoder ensemble stacking model

加速康复背景下老年髋部骨折患者围手术期医院感染的影响因素研究

在线全文

学校读者我要写书评

暂无评论

智慧健康 2024年第13期10卷 141-143,147页

作者：管裕强罗家俊张政委张凌峰彭志高梅州铁炉桥医院骨伤科广东梅州514700

目的分析加速康复背景下老年髋部骨折患者围手术期医院感染的影响因素。方法对本院2020年1月—2022年12月开展加速康复管理后收治的200例进行手术治疗的老年髋部骨折患者临床资料进行回顾性分析,统计其手术治疗后围手术期医院感染发生情... 详细信息

目的分析加速康复背景下老年髋部骨折患者围手术期医院感染的影响因素。方法对本院2020年1月—2022年12月开展加速康复管理后收治的200例进行手术治疗的老年髋部骨折患者临床资料进行回顾性分析,统计其手术治疗后围手术期医院感染发生情况,根据其是否发生围手术期医院感染进行分组,并且采集其临床资料,对影响其发生围手术期医院感染的相关因素进行单因素分析和多因素Logistic分析。结果200例患者中共计16例(8.00%)发生围手术期医院感染,16例患者中,下呼吸道感染患者、泌尿道感染患者、手术部位感染患者分别8例(50.00%)、5例(31.25%)和3例(18.75%)。发生围手术期医院感染患者在年龄、住院时间、合并慢性病、下肢动脉硬化闭塞症、心功能不全、留置尿管、骨牵引及老年痴呆方面和未发生围手术期医院感染患者存在明显差异(P<0.05)。多因素Logistic回归分析结果显示,合并下肢动脉硬化闭塞症、心功能不全、留置尿管、骨牵引及老年痴呆是影响患者发生围手术期医院感染的独立危险因素(P<0.05)。结论在加速康复背景下,老年髋部骨折患者手术治疗期间具有一定的围手术期医院感染风险,其中合并下肢动脉硬化闭塞症、心功能不全、留置尿管、骨牵引及老年痴呆是影响其发生围手术期医院感染的独立危险因素,临床需要在加速康复外科理念下对患者围术期管理措施作进一步优化,以在更大程度上提高患者围手术期医院感染的预防效果。

关键词：加速康复背景老年髋部骨折围手术期医院感染影响因素

Excellent energy storage performance in Bi_(0.5)Na_(0.5)TiO_(3)-based lead-free high-entropy relaxor ferroelectrics via B-site modification

在线全文

学校读者我要写书评

暂无评论

Journal of Advanced Ceramics 2024年第3期13卷 345-353页

作者： Kaihua Yang Gengguang luo Li Ma Ruoxuan Che Zhiyi Che Qin Feng Zhenyong Cen Xiyong Chen jiajun Zhou Nengneng luo State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures School of ResourcesEnvironment and MaterialsGuangxi UniversityNanning 530004China School of Optical and Electronic Information Key Lab of Functional Materials for Electronic Information(B)of Ministry of EducationHuazhong University of Science and TechnologyWuhan 430074China

Next-generation advanced high/pulsed power capacitors urgently require dielectric materials with outstanding energy storage ***_(0.5)Na_(0.5)TiO_(3)-based lead-free materials exhibit high polarization,but the high remanent polarization and large polarization hysteresis limit their applications in dielectric ***,high-entropy perovskite relaxor ferroelectrics(Na_(0.2)Bi_(0.2)Ba_(0.2)Sr_(0.2)Ca_(0.2))(Ti1-x%Zrx%)O_(3)are designed by adding multiple ions in the A-site and replacing the B-site Ti^(4+)with a certain amount of Zr^(4+).The newly designed system showed high relaxor feature and slim polarization-electric(P-E)***,improved relaxor feature and obviously delayed polarization saturation were found with the increasing of Zr^(4+).Of particular importance is that both high recoverable energy storage density of 6.6 J/cm^(3) and energy efficiency of 93.5%were achieved under 550 kV/cm for the ceramics of x=6,accompanying with excellent frequency stability,appreciable thermal stability,and prosperous discharge *** work not only provides potential dielectric materials for energy storage applications,but also offers an effective strategy to obtain dielectric ceramics with ultrahigh comprehensive energy storage performance to meet the demanding requirements of advanced energy storage applications.

关键词： energy storage density (Bi_(0.5)Na_(0.5))TiO_(3) high-entropy perovskite ceramics polarization saturation relaxor ferroelectrics(RFEs)

In vivo evaluation of additively manufacturedmulti-layered scaffold for the repair of large osteochondral defects

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2022年第3期5卷 481-496页

作者： Maryam Tamaddon Gordon Blunn Rongwei Tan Pan Yang Xiaodan Sun Shen-Mao Chen jiajun luo Ziyu Liu Ling Wang Dichen Li Ricardo Donate Mario Monzón Chaozong Liu Institute of Orthopaedic and Musculoskeletal Science Royal National Orthopaedic HospitalUniversity College LondonStanmore HA74LPUK School of Pharmacy and Biomedical Sciences University of PortsmouthPortsmouth PO12DTUK Guangdong Engineering Research Center of Implantable Medical Polymer Shenzhen Lando Biomaterials Co.Ltd.Shenzhen 518107China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China State Key Laboratory for Manufacturing System Engineering School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710054China Departamento de Ingeniería Mecánica Grupo de Investigación en Fabricación Integrada y AvanzadaUniversidad de Las Palmas de Gran CanariaCampus Universitario de Tafira s/n35017 Las PalmasSpain

The repair of osteochondral defects is one of the major clinical challenges in ***-established osteochondral tissue engineering methods have shown promising results for the early treatment of small ***,less success has been achieved for the regeneration of large defects,which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the *** this study,we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion *** developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen“sandwich”composite *** microstructure and mechanical properties of the scaffold were examined,and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle *** 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen–HAp scaffold,and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage,as demonstrated by hyaline-like cartilage *** histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control *** genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control *** findings showed the safety and efficacy of the cell-free“translation-ready”osteochondral scaffold,which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects.

关键词： Osteochondral scaffold Large animal Additive manufacturing Porous titanium

Hydrogel bioadhesives harnessing nanoscale phase separation for Achilles tendon repairing

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第2期17卷 778-787页

作者： Jun Zhang Xingmei Chen Jingseng Lin Pei Zhang Iek Man Lei Yue Tao jiajun Zhang Tian luo Ji Liu Department of Mechanical and Energy Engineering Southern University of Science and TechnologyShenzhen 518055China Research and Innovation Center SKG Health TechnologyShenzhen 518000China SAFE(Shenzhen)Medical Technology Company Limited Shenzhen 518000China Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems Department of Mechanical and Energy EngineeringSouthern University of Science and TechnologyShenzhen 518055China Guangdong Provincial Key Laboratory of Human-Augmentation and Rehabilitation Robotics in Universities Southern University of Science and TechnologyShenzhen 518055China

Repairing Achilles tendon has emerged as a long-standing challenge in the orthopaedic *** suture is the gold standard for re-attaching and repairing the fractured Achilles tendons in clinical surgeries,it is still subjected to numerous adverse side-effects,including chronic inflammatory,tendon tissue re-rupture,scar formation,and post-surgical peritendinous *** this work,we develop a class of hydrogel bioadhesives with tailored nanoscale phase separation for Achilles tendon *** address the existing limitations of sutures,our hydrogel bioadhesives encompass three core functionalities:(i)instant and tough adhesion to Achilles tendon tissues,(ii)extraordinary long-term adhesion robustness under wet and dynamic in vivo conditions,and(iii)anti-postsurgical peritendinous *** our hydrogel bioadhesives with sutures,such kind of integrated approach enables a conformable yet robust biointerface with the tendon tissues,and prevents the fibroblast migration and formation of connective tissues,thus facilitating the tendon *** hydrogel bioadhesives reported here open up new opportunities for the repairing of fractured Achilles tendons in diverse and complicated clinical scenarios.

关键词： hydrogels phase separation bioadhesion robustness tendon repairing