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Food Science and Human Wellness 2023年第6期12卷 2369-2380页

作者： Xiao Hu Yujiao Qian Zhipeng Gao Gaoyang Li Fuhua Fu jiajing guo Yang Shan Hunan Agriculture Product Processing Institute International Joint Lab on Fruits&Vegetables ProcessingQuality and SafetyHunan Key Lab of Fruits&Vegetables StorageProcessingQuality and SafetyHunan Academy of Agricultural SciencesChangsha 410125China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China

The microbial potential of Penicillium has received critical *** present research aimed to elucidate the efficacy of crude enzyme secreted from Penicillium oxalicum WX-209 in degrading citrus segments and evaluate the safety of the *** showed that citrus segment membranes gradually dissolved after treatment with the crude enzyme solution,indicating good degradation *** significant differences in body weight,food ingestion rate,hematology,blood biochemistry,and weight changes of different organs were found between the enzyme intake and control *** experiments showed that the crude enzyme had high biological ***,the whole genome of *** WX-209 was sequenced by PacBio and Illumina ***-five scaffolds were assembled to generate 36 Mbp size of genome sequence comprising 11369 predicted genes modeled with a GC content of 48.33%.A total of 592 genes were annotated to encode enzymes related to carbohydrates,and some degradation enzyme genes were identified in strain *** WX-209.

关键词： Penicillium oxalicum WX-209 Crude enzyme Degradation Safety evaluation Genome sequencing

Membrane-camouflaged supramolecular nanoparticles for co-delivery of chemotherapeutic and molecular-targeted drugs with siRNA against patient-derived pancreatic carcinoma

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Acta Pharmaceutica Sinica B 2022年第8期12卷 3410-3426页

作者： Honglin Tang Yanan Xue Bowen Li Xiaojie Xu Fu Zhang jiajing guo Qijun Li Tingting Yuan Yuan Chen Yubin Pan Yuan Ping Da Li Department of Medical Oncology Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310058China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310058China Department of Hepatobiliary and Pancreatic Surgery the First Affiliated HospitalZhejiang UniversityHangzhou 310003China

Pancreatic cancer remains one of the most lethal malignancies worldwide. The combination of the first-line standard agent gemcitabine(GEM) with the molecular-targeted drug erlotinib(Er) has emerged as a promising strategy for pancreatic cancer treatment. However, the clinical benefit from this combination is still far from satisfactory due to the unfavorable drug antagonism and the fibrotic tumor microenvironment. Herein, we propose a membrane-camouflaged dual stimuliresponsive delivery system for the co-delivery of GEM and Er into pancreatic cancer cells and tissues to block the antagonism, as well as reshapes profibrotic tumor microenvironment via simultaneous delivery of small interference RNA(siRNA) for synergistic pancreatic cancer treatment. This “all-in-one”delivery system exhibits sensitive GSH and pH-dependent drug release profiles and enhances the inhibitory effects on the proliferation and migration of tumor cells in vitro. Excitingly, the systemic injection of such a biomimetic drug co-delivery system not only resulted in superior inhibitory effects against orthotopic pancreatic tumor and patient-derived tumor(PDX), but also greatly extended the survival rate of tumor-bearing mice. Our findings provide a promising therapeutic strategy against pancreatic cancer through the enhanced synergistic effect of target therapy, chemotherapy and anti-fibrotic therapy, which represents an appealing way for pancreatic cancer treatment.

关键词： Chemotherapy Target therapy siRNA Nanomedicine Hybrid membrane Drug antagonism Pancreatic carcinoma Patient-derived tumor

Dual-ratiometric magnetic resonance tunable nanoprobe with acidic-microenvironment-responsive property to enhance the visualization of early tumor pathological changes

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Nano Research 2023年第7期16卷 10034-10046页

作者： Rong Cao Ning Tang Yi Zhu An Chen Yumeng Li Renbin Ge Yuan Li Zhongyi Huang jiajing guo Jiali Deng Hongwei Lu Ziwei Lu Helen Forgham Thomas PDavis Ruirui Qiao Zhongling Wang Department of Radiology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China School of food science Nanjing Xiaozhuang UniversityNanjing 211171China School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai 200093China Department of Neurology School of MedicineNew York UniversityNew York 10016USA Department of Biomedical Engineering College of EngineeringShantou UniversityShantou 515063China Department of Radiology The First Affiliated Hospital of Soochow UniversitySuzhou 215006China Australian Institute for Bioengineering and Nanotechnology The University of QueenslandBrisbane QLD 4072Australia

The development of microenvironment-responsive nanoprobes has shown great promise for use in magnetic resonance imaging(MRI),with the advantage of significantly improved specificity and good ***,the clinical application of responsive probes is hampered by a lack of biological sensitivity for early molecular diagnostics and visualizing microenvionment of metabolism reprogramming in tumor ***,we report on a dual-ratiometric magnetic resonance tunable(DMRT)nanoprobe designed by crosslinking different ratios of transferrin chelating gadolinium and superparamagnetic nanoparticles,complexed to a pH responsive biocompatible *** dually activatable nanoprobe enables pH-dependent tumor microenvironment visualization,providing exceptional quantitative pathophysiological information in vitro and in *** used in combination with dual-contrast enhancement triple subtraction imaging technique(DETSI),this smart nanoprobe guarantees the diagnosis of early-stage *** envisage that this novel integrated nanoplatform will provide a new paradigm for the clinical translation of robust DMRT nanoprobes for early disease detection and staging,as well as microenvironment visualization and disease progression monitoring.

关键词： ratiometric nanoprobe dually activatable microenvironment visualization early diagnosis pathological changes

A rapid-response smart nanoplatform with dual T_(1)-T_(2) activation for acidic microenvironment imaging

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Science China Materials 2024年第12期67卷 4101-4110页

作者： Meng Luo jiajing guo Yi Zhu Jiali Deng Hongwei Lu Lei Li Zhongling Wang Department of Medical Imaging The First Affiliated Hospital of Kangda College of Nanjing Medical University/The First People’s Hospital of LianyungangLianyungang 222000China Department of Radiology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai 200093China Department of Biomedical Engineering College of EngineeringShantou UniversityShantou 515063China

Acidity-activatable magnetic resonance imaging(MRI)nanoprobes offer great potential for in vivo cancer imaging by targeting the acidic tumor microenvironment(TME).However,their effectiveness is limited by the delayed response at tumor sites and uncontrollable background noise,compromising imaging accuracy and ***,an acidic TME-responsive nanoprobe,SPIO@ZIF-8@Gd(SZG),with dually activatable T_(1) and T_(2) MR signals is shown for acidity-selective contrast enhancement in a rapid response *** shows decreased T_(1) and T_(2) contrast intensity in normal physiological *** targeting acidic TME,the zeolitic imidazolate framework-8(ZIF-8)layer undergoes instantaneous decomposition,releasing Gd^(3+)(T_(1)-weighted),and exposing the inner SPIO(T_(2)-weighted)core,thereby sequentially recovering the *** to previously reported T_(1)-T_(2) nanoprobes,SZG demonstrates noticeable“dual activation”after just 30 min and reaches its peak 4 h after acid ***,it shows an excellent“acidity correlation”between relaxation times and pH *** the SZG nanoprobe is used combined with“dual-contrast enhanced subtraction(DESI)”,the contrast difference between diseased and normal tissue can be increased by 10 times,which is significantly higher than traditional single-mode T_(1)/T_(2) contrast ***,these findings demonstrate a rapid imaging strategy of dual-activation MR imaging of the acidic TME and simultaneous background suppression,thus paving the way for precise tumor malignancy differentiation,early tumor detection,and accurate tumor grading.

关键词： rapid response zeolitic imidazolate framework-8 dual T_(1)-T_(2)activation acidic tumor microenvironment

Crude enzyme derived from Penicillium oxalicum WX-209: degrading capability of citrus segment， safety evaluation， and prediction of enzyme- producing genes by whole genome sequencing

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Food Science and Human Wellness 2022年

作者： Xiao Hu Yujiao Qian Zhipeng Gao Gaoyang Li Fuhua Fu jiajing guo Yang Shan Hunan Agriculture Product Processing Institute， International Joint Lab on Fruits &Vegetables Processing， Quality and Safety， Hunan Key Lab of Fruits &Vegetables Storage， Processing， Quality and Safety， Hunan Academy of Agricultural Sciences College of Animal Science and Technology， Hunan Agricultural University

The microbial potential of Penicillium has received critical attention. The present research aimed to elucidate the efficacy of crude enzyme secreted from Penicillium oxalicum WX-209 in degrading citrus segments and evaluate the safety of the process. Results showed that citrus segment membranes gradually dissolved after treatment with the crude enzyme solution， indicatinggood degradation capability. No significant differences in body weight， food ingestion rate， hematology， blood biochemistry， and weight changes of different organs were found between the enzyme intake and control groups. Serial experiments showed that the crude enzyme had high biological safety. Moreover， the whole genome of P. oxalicum WX-209 was sequenced by PacBio and Illumina platforms. 25 scaffolds were assembled to generate 36 Mbp size of genome sequence comprising 11，369 predicted genes modeled with a GC content of 48.33%. A total of 592 genes were annotated to encode enzymes related to carbohydrates， and some degradation enzyme genes were identified in strain P. oxalicum WX-209.

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蓝莓花色苷生理活性、提取纯化及稳态化研究进展

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中国食品学报 2024年第2期24卷 407-417页

作者：李梓旋郭佳婧苏东林单杨湖南大学生物学院隆平分院长沙410125 湖南省农业科学院农产品加工研究所、果蔬贮藏加工与质量安全湖南省重点实验室长沙410125

花色苷是蓝莓中的主要酚类活性成分,因其良好的抗氧化、抗炎、抗肿瘤、护眼等活性而备受关注,然而花色苷容易受到光照、氧气、pH值、金属离子等因素的影响,且人体吸收利用率低,限制了其应用。本文综述了蓝莓花色苷的功能活性、提取纯化... 详细信息

花色苷是蓝莓中的主要酚类活性成分,因其良好的抗氧化、抗炎、抗肿瘤、护眼等活性而备受关注,然而花色苷容易受到光照、氧气、pH值、金属离子等因素的影响,且人体吸收利用率低,限制了其应用。本文综述了蓝莓花色苷的功能活性、提取纯化方法及稳态化技术,为蓝莓花色苷在食品领域的应用提供参考。

关键词：蓝莓花色苷功能活性提取纯化稳态化

中小企业金融健康问题研究——基于伊犁州直“专精特新”中小企业问卷调查

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金融发展评论 2023年第2期 52-64页

作者：郭佳靖中国人民银行伊犁州中心支行

中小企业是国民经济和社会发展的基石,也是普惠金融的重点服务对象之一,近年来,我国普惠金融体系不断完善,中小企业金融服务可得性和覆盖率大幅提升。然而,普惠金融并不总是产生好的结果,其是否以及如何改善中小企业福祉成为新时代推动... 详细信息

中小企业是国民经济和社会发展的基石,也是普惠金融的重点服务对象之一,近年来,我国普惠金融体系不断完善,中小企业金融服务可得性和覆盖率大幅提升。然而,普惠金融并不总是产生好的结果,其是否以及如何改善中小企业福祉成为新时代推动普惠金融高质量发展需要关注的问题。为此,本文引入金融健康概念,在探讨构建中小企业金融健康指标体系和测算方法的基础上,以伊犁州直“专精特新”中小企业为例,评估中小企业金融健康现状,指出当前中小企业金融健康存在的问题,提出政策建议,以期促进我国普惠金融和中小企业高质量发展。

关键词：中小企业问卷调查普惠金融金融健康

大肠杆菌表达类黄酮O-甲基转移酶合成槲皮素甲基化衍生物

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食品与生物技术学报 2022年第7期41卷 111-119页

作者：田苗苗郭佳婧刘娟单杨湖南省农业科学院农产品加工研究所/果蔬贮藏加工与质量安全湖南省重点实验室/湖南省果蔬加工与质量安全国际科技创新合作基地湖南长沙410125 湖南大学隆平分院长沙410082

槲皮素是一种重要的柑橘类黄酮,具有抗菌、抗炎和抗氧化等生物学活性,但水溶性和脂溶性较差,研究表明可通过甲基化提高其代谢稳定性和生物利用度,目前微生物转化法是获得甲基化槲皮素的良好方法。作者筛选了11种不同来源的类黄酮O-甲基... 详细信息

槲皮素是一种重要的柑橘类黄酮,具有抗菌、抗炎和抗氧化等生物学活性,但水溶性和脂溶性较差,研究表明可通过甲基化提高其代谢稳定性和生物利用度,目前微生物转化法是获得甲基化槲皮素的良好方法。作者筛选了11种不同来源的类黄酮O-甲基转移酶(FOMT),并根据甲基化位点进行分类构建相应的大肠杆菌工程菌,以槲皮素为底物进行发酵分别合成了4种单O-甲基槲皮素(柽柳黄素、鼠李素、异鼠李素和3-O-甲基槲皮素),最高产量分别为31.17、11.17、8.90、52.95 mg/L。随后构建了大肠杆菌共培养体系,分步添加含有MpOMT4和OsNOMT的重组大肠杆菌全细胞催化剂,并通过调整体系中两种菌体的比例和生物量,最终确定菌体细胞质量浓度为24 g/L(以细胞干质量计)、两种菌体质量比为1∶2时,4′,7-二甲氧基槲皮素的最高产量为21.56 mg/L。

关键词：槲皮素类黄酮O-甲基转移酶(FOMT) 槲皮素甲基化衍生物生物合成

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我国金融消费者权益保护现状及经验借鉴

金融发展评论 2022年第2期 82-95页

作者：郭佳靖中国人民银行伊犁州中心支行

加强金融消费者权益保护成为后危机时代国际金融监管改革的重点之一。近年来,我国对金融消费者权益保护工作越来越重视,形成了“一委一行两会”格局下金融消费者权益保护“内双峰”模式,金融消费者权益保护工作机制体制不断完善。本文... 详细信息

加强金融消费者权益保护成为后危机时代国际金融监管改革的重点之一。近年来,我国对金融消费者权益保护工作越来越重视,形成了“一委一行两会”格局下金融消费者权益保护“内双峰”模式,金融消费者权益保护工作机制体制不断完善。本文在系统总结分析我国金融消费者权益保护工作现状和不足的基础上,积极借鉴美国、英国和澳大利亚等发达国家在金融消费者权益保护立法、监管机构设置、金融纠纷多元化解机制建设、金融消费者教育等方面的实践经验,提出政策建议,以期促进完善我国金融消费者权益保护。

关键词：金融消费者权益保护制度建设金融消费纠纷金融消费者教育

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疾控机构信息化建设的现状及对策

福建电脑 2020年第5期36卷 75-76页

作者：郭佳静泉州市丰泽区疾病预防控制中心福建泉州362000

信息技术已渗透到中国生产生活的各个方面,特别是在卫生系统发展迅速。县级疾控机构存在资金不足、缺乏专业人员等问题。为了促进疾控中心信息化建设的快速发展,必须给予足够的关注,建立和完善管理机制,加强网络基础设施建设,提高员工... 详细信息

信息技术已渗透到中国生产生活的各个方面,特别是在卫生系统发展迅速。县级疾控机构存在资金不足、缺乏专业人员等问题。为了促进疾控中心信息化建设的快速发展,必须给予足够的关注,建立和完善管理机制,加强网络基础设施建设,提高员工的专业素质,提升网络安全系数和信息的利用率。本文探讨了我国疾病预防控制中心信息化建设的现状和对策。

关键词：疾控机构信息化管理