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Zoological Research 2016年第2期37卷 75-83页

作者： jia-qi wang jia-jia wang Xu-jian WU Wei-Hong ZHENG Jin-Song LIU School of Life and Environmental Sciences Wenzhou University Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection

Environmental cues play important roles in the regulation of an animal＇s physiology and behavior. One such cue, photoperiod, plays an important role in the seasonal acclimatization of birds. It has been demonstrated that an animal＇s body mass, basal metabolic rate （BMR）, and energy intake, are all affected by photoperiod. The present study was designed to examine photoperiod induced changes in the body mass, metabolism and metabolic organs of the silky starling, Stumus sericeus. Captive silky starlings increased their body mass and BMR during four weeks of acclimation to a short photoperiod. Birds acclimated to a short photopedod also increased the mass of certain organs （liver, gizzard and small intestine）, and both gross energy intake （GEl） and digestible energy intake （DEI）, relative to those acclimated to a long photoperiod. Furthermore, BMR was positively correlated with body mass, liver mass, GEl and DEI. These results suggest that silky starlings increase metabolic thermogenesis when exposed to a short photoperiod by increasing their body and metabolic organ mass, and their GEl and DEI. These findings support the hypothesis that bird species from temperate climates typically display high phenotypic flexibility in thermogenic capacity.

关键词： Basal metabolic rate (BMR) Body mass Energy budget Organ mass Photoperiod Silkystarling Sturnus sericeus.

An iterative computation method for interpreting and extending an analytical battery model

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第2期9卷 279-288页

作者： Neng-gan ZHENG Zhao-hui WU Man LIN qi-jia wang School of Computer Science and Technology Zhejiang University Hangzhou 310027 China Department of Computer Science St. Francis Xavier University Antigonish NS B2G 2 W5 Canada

Battery models are of great importance to develop portable computing systems,for whether the design of low power hardware architecture or the design of battery-aware scheduling *** this paper,we present a physically justified iterative computing method to illustrate the discharge,recovery and charge process of Li/Li-ion *** discharge and recovery processes correspond well to an existing accurate analytical battery model:R-V-W's analytical model,and thus interpret this model *** method can also extend R-V-W's model easily to accommodate the charge *** work will help the system designers to grasp the characteristics of R-V-W's battery model and also,enable to predict the battery behavior in the charge process in a uniform way as the discharge process and the recovery *** are performed to show the ac-curacy of the extended model by comparing the predicted charge times with those derived from the DUALFOIL *** profiles with different combinations of battery modes were *** experimental results show that the extended battery model preserves high accuracy in predicting the charge behavior.

关键词： Analytical battery model lterative computation method Capacity response Charge Discharge

The interaction effect of grip strength and lung function (especially FVC) on cardiovascular diseases: a prospective cohort study in jiangsu Province, China

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Journal of Geriatric Cardiology 2022年第9期19卷 651-659页

作者： jia-Li LIU jia-qi wang Dan wang Yu qiN Yong-qing ZHANG Quan-Yong XIANG School of Public Health Southeast UniversityNanjingJiangsu ProvinceChina Department of Chronic Non-com-municable Disease Control Jiangsu Province Center for Disease Control and PreventionNanjingJiangsu ProvinceChina

OBJECTIVE Lung function and grip strength(GS)are associated with cardiovascular disease(CVD),but whether these risk factors interact to affect CVD is *** study aimed to explore the interactions between lung function and GS with major CVD(defined as fatal/non-fatal myocardial infarction,stroke,and heart failure)*** We conducted a prospective cohort study on the Chinese population in jiangsu *** proportional haz-ards models were used to explore the associations between GS,lung function,and major CVD *** A total of 5967 participants were included in our study;among them,182 participants developed major ***-cipants with low forced vital capacity(FVC)had a higher risk of major CVD(hazard ratio(HR)=1.45;95%confidence interval(CI):1.05-2.01;P<0.05)compared with normal *** risk of major CVD incidence(HR=0.54;95%CI:0.35-0.83;P<0.01)was significantly lower in participants with high GS than in individuals with low *** interaction between FVC and GS for major CVD incidence(P=0.006)was statistically *** with normal FVC participants with high GS,low FVC parti-cipants with low GS had the highest risk of major CVD incidence(HR=2.50;95%CI:1.43-4.36;P<0.01).CONCLUSION Among people with low FVC,the risk of major CVD is lower with high *** with low FVC and low GS have the highest risk of major ***,more attention should be paid to the incidence of major CVD in individu-als with low FVC,especially those who have lower GS.

关键词： function strength interaction

Rapid Inactivation of Chloroplastic Ascorbate Peroxidase is Responsible for Oxidative Modification to Rubisco in Tomato (Lycopersicon esculentum) under Cadmium Stress

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Journal of Integrative Plant Biology 2008年第4期50卷 415-426页

作者： Kai-Lang Liu Lin Shen jia-qi wang Ji-Ping Sheng College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 China

To investigate the sensitive site of antioxidant systems in chloroplast under cadmium stress and its consequence on reactive oxygen species production and action, the sub-organellar localization of chloroplast superoxide dismutases （SOD, EC 1.***.1.1） and ascorbic peroxidase （APX, EC 1.***.1.11） isoenzymes and changes of enzymes activities under cadmium stress were investigated in tomato seedlings. Two APX isoforms, one thylakoid-bound and one stromal, were detected. Cd at 50 μM induced a moderate increase of SOD activities but a rapid inactivation of both APX isoenzymes. APX inactivation was mainly related to the decrease of ascorbate concentration, as supported by in vitro treatment of exogenous ascorbate and APX kinetic properties under Cd stress. H2O2 accumulation in chloroplast, as a consequence of APX inactivation, was associated with a 60% loss of Rubisco （EC 4.***.1.39） activity, which could be partially accounted for by a 10% loss of Rubisco content. Protein oxidation assay found that the Rubisco large subunit was the most prominent carbonylated protein; the level of carbonylated Rubisco large subunit increased fivefold after Cd exposure. Thiol groups in the Rubisco large subunit were oxidized, as indicated by non-reducing electrophoresis. Treating crude extract with H2O2 resulted in a similar pattern of protein oxidation and thiols oxidation with that observed in Cd-treated plants. Our study indicates that APXs in the chloroplast is a highly sensitive site of antioxidant systems under Cd stress, and the inactivation of APX could be mainly responsible for oxidative modification to Rubisco and subsequent decrease in its activity.

关键词： ascorbate peroxidase cadmium hydrogen peroxide protein oxidation Rubisco.

Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

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Zoological Research 2016年第5期37卷 301-306页

作者： Chun-Lei wang Zhi-Peng wang jia-qi wang Ming-You Li Xiao-Wu CHEN Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China

Piwi-interacting RNA （piRNA） plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder （Paralichthys ofivaceus） were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters （PRCs） were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.

关键词： Paralichthys olivaceus Ovary Testis piRNA

Effect of ephrin-B2 on the expressions of angiopoietin-1 and-2 after focal cerebral ischemia/reperfusion

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Neural Regeneration Research 2016年第11期11卷 1784-1789页

作者： Hui Xiao qing Huang jia-qi wang qing-qing Deng Wen-ping Gu Department of Neurology Changsha Municipal Central Hospital Department of Neurology Xiangya HospitalCentral South University

Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of local cerebral ischemia was prepared by focal middle cerebral artery occlusion, followed by 24-hour reperfusion. Then, ephrin-B2 protein was administered intracerebroventricularly for 3 consecutive days via a micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated the expression levels of angiopoietin-1 （Ang-1） mRNA and protein in the penumbra cortex of the ephrin-B2 treated group were decreased at day 4 after reperfusion, and increased at day 28, while the expression levels of angiopoietin-2 （Ang-2） were highly up-regulated at all time points tested. Double immunofluorescent staining indicated that Ang-1 and Ang-2 were both expressed in vascular endothelial cells positive for CD31. These findings indicate that ephrin-B2 influences the expressions of Ang-1 and Ang-2 during angiogenesis following transient focal cerebral ischemia.

关键词： nerve regeneration focal cerebral ischemia/reperfusion ephrin-B2 angiogenesis angiopoietin-1 angiopoietin-2 neuroprotection

A Novel Gait Pattern Recognition Method Based on LSTM-CNN for Lower Limb Exoskeleton

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Journal of Bionic Engineering 2021年第5期18卷 1059-1072页

作者： Chao-feng Chen Zhi-jiang Du Long He Yong-jun Shi jia-qi wang Wei Dong State Key Laboratory of Robotics and System Harbin Institute of Technology(HIT)Harbin 150001China China South Industries Group Corporation Weapon Equipment Research InstituteBeijing 102202China

This paper describes a novel gait pattern recognition method based on Long Short-Term Memory(LSTM)and Convolutional Neural Network(CNN)for lower limb *** Inertial Measurement Unit(IMU)installed on the exoskeleton to collect motion information,which is used for LSTM-CNN *** article considers five common gait patterns,including walking,going up stairs,going down stairs,sitting down,and standing *** the LSTM-CNN model,the LSTM layer is used to process temporal sequences and the CNN layer is used to extract *** optimize the deep neural network structure proposed in this paper,some hyperparameter selection experiments were carried *** addition,to verify the superiority of the proposed recognition method,the method is compared with several common methods such as LSTM,CNN and *** results show that the average recognition accuracy can reach 97.78%,which has a good recognition eff ***,according to the experimental results of gait pattern switching,the proposed method can identify the switching gait pattern in time,which shows that it has good real-time performance.

关键词： Lower limb exoskeleton Gait pattern recognition LSTM-CNN Recognition accuracy Real-time performance

Evaluating the immune responses of mice to subcutaneous immunization with Helicobacter pylori urease B subunit

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Journal of Animal Science and Biotechnology 2014年第2期5卷 181-187页

作者： Peng Sun jia-qi wang Yu-Tao Zhang Sheng-Guo Zhao State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesNo.2 Yuanmingyuan West RoadBeijing 100193P.R.China

Background： Helicobacter pylori, a gram-negative bacterial pathogen that expresses a strong urease activity, is associated with the development of gastroduodenal disease. Urease B subunit, one of the two structural subunits of urease, was expressed in E. coil BL21 （DE3） strain. The objective of this study was to evaluate the effects of He/icobacter py/ori urease B subunit on the immune responses in mice by subcutaneous immunization. Methods： The mice were immunized and boosted with Helicobacter pylori urease B subunit antigen subcutaneously three times with 2-wk intervals between the immunizations and boosters. The mice in the control group were immunized with PBS. The adjuvant group received PBS containing complete/incomplete freund＇s adjuvant identical to antigen group without Helicobocter pylori urease B subunit antigen. Four weeks after the final booster, all the mice were sacrificed. Blood was collected on d 0, 14, 28 and 56 before immunization, booster and sacrifice, respectively. Immediately after sacrifice, gastric liquid and spleen were collected for antibody and cytokine analyses. Results： Urease B subunit increased the concentrations of serum and gastric anti-urease B antigen specific IgG, and the levels of interteukin-4 and interferon-y in splenocytes of the mice （P 〈 0.05）. Conclusions： This study demonstrated that recombinant responses in mice by subcutaneous immunization, which against Helicobocter pylori. urease B subunit can induce systemic and local immune might be used as the effective component of vaccine

关键词： Antibody Cytokines Helicobacter pylori urease B subunit Mice

Factors influencing the performance of paintable carbon-based perovskite solar cells fabricated in ambient air

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Chinese Physics B 2018年第3期27卷 508-514页

作者： Wei-Kang Xu Feng-Xiang Chen Gong-Hui Cao jia-qi wang Li-Sheng wang Department of Physics Science and Technology School of Science Wuhan University of Technology Wuhan 430070 China

To date, many efforts have been made to improve the performance of paintable carbon-based （PC-based） perovskite solar cells （PSCs）. Though great progress has been achieved, their power conversion efficiencies are still relatively low compared with hole-transport-materials-based PSCs. General research on influencing factors of performance in PC-based PSCs is still insufficient. In this work, PC-based PSCs were fabricated in ambient air and four groups of controlled experi- ments were performed in which the PbI2 layers were prepared with or without antisolvent extraction treatment. These four groups of experiments were designed to find out the effect of different influencing factors on PC-based PSCs performance, for example, PbI2 residual, the surface morphology of the perovskite film, the surface roughness of the perovskite film, and the contact status of the perovskite/carbon electrode interface. With a systematic analysis, we demonstrated that the contact status of the perovskite/carbon electrode interface played a vital role in PC-based PSCs, and a fiat, smooth perovskite surface could help to improve this contact status significantly. Besides, on the precondition of a poor contact interface, no PbI2 residual and a good surface morphology only brought limited benefits to the performances of PC-based PSCs.

关键词： influencing factors paintable carbon-based perovskite solar cells contact status

Numerical studies on issues of Re-independence for indoor airflow and pollutant dispersion within an isolated building

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Building Simulation 2022年第7期15卷 1259-1276页

作者： Peng-Yi Cui Wei-qiu Chen jia-qi wang Jin-Hao Zhang Yuan-Dong Huang Wen-Quan Tao College of Environment and Architecture University of Shanghai for Science and TechnologyShanghaiChina Key Laboratory of Thermo-Fluid Science and Engineering of MOE School of Energy and Power EngineeringXi'an Jiaotong UniversityXi’anChina

This study conducted the numerical models validated by wind-tunnel experiments to investigate the issues of Re-independence of indoor airflow and pollutant dispersion within an isolated *** window Reynolds number(Re_(w))was specified to characterize the indoor flow and *** indicators of RRC(ratio of relative change)or DR(K_DR)(difference ratio of dimensionless concentration)<5%were applied to quantitatively determine the critical Rew for indoor flow and turbulent *** results show that the critical Re(Re_(crit)) value is position-dependent,and Re_(crit) at the most unfavorable position should be suggested as the optimal value within the whole areas of *** Re(H,orit)=27,000 is recommended for the outdoor flows;while Re_(w,crit)=15,000 is determined for the indoor flows due to the lower part below the window showing the most *** suggested Re_(w,crit)(=15,000)for indoor airflow and cross ventilation is independence of the window ***,taking K_DR≤5% as the indicator,the suggested Re_(w,crit) for ensuring indoor pollutant diffusion enter the independence regime should also be 15,000,indicating that indoor passive diffusion is completely determined by the flow *** contours of dimensionless velocity(U/U_(0))and concentration(K)against the increasing Re(w) further confirmed this critical *** study further reveals the Re-independence issues for indoor flow and dispersion to ensure the reliability of the data obtained by reduced-scale numerical or wind-tunnel models.

关键词： CFD simulation Re-independence similarity criterion indoor ventilation pollutant dispersion