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RNA interference-mediated silencing of a Halloween gene spookier affects nymph performance in the small brown planthopper Laodelphax striatellus

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Insect Science 2015年第2期22卷 191-202页

作者： Shuang jia pin-Jun Wan Li-Tao Zhou Li-Li Mu Guo-Qing Li Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant Protection Nanjing AgriculturalUniversity Nanjing China

Post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. Halloween gene spookier （spok, cyp307a2） has been documented to be involved in ecdysteroidogenesis in Drosophila melanogaster and Bombyx mori. We describe here the cloning and characterization of Halloween gene spookier （Lsspok, Lscyp307a2） in the small brown planthopper Laodelphax striatellus, a hemipteran insect species. LsSPOK has three insect-conserved P450 motifs, that is, Helix-K, PERF motif and heme-binding domain. Temporal and spatial expression patterns of Lsspok were evaluated by quantitative polymerase chain reaction. Through the fouth- instar and the early fifth-instar stages, Lsspok showed two expression peaks in the second- and fifth-day fourth-instar nymphs, and two troughs in the first-day fourth and fifth in- stars. On day 5 of the fourth-instar nymphs, Lsspok clearly had a high transcript level in the thorax where prothoracic glands were located. Dietary introduction of double-stranded RNA of Lsspok in the nymph stage successfully knocked down the target gene, decreased expression level ofecdysone receptor （LsEcR） gene, caused nymphal lethality and delayed development. Ingestion of 20-hydroxyecdysone in Lsspok-dsRNA-exposed nymphs did not increase Lsspok expression level, but almost completely rescued the LsEcR mRNA level and relieved the negative effects on survival and development. Thus, our data suggest that the ecdysteroidogenic pathway is conserved in insects and LsSPOK is responsible for specific steps in ecdysteroidogenesis in L. striatellus.

关键词： development double-stranded RNA ecdysteroidogenesis Laodelphaxstriatellus lethality spookier

A semi-analytical pressure and rate transient analysis model for inner boundary and propped fractures exhibiting dynamic behavior under long-term production conditions

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Petroleum Science 2024年第4期21卷 2520-2535页

作者： Lin-Song Cheng Chong Cao Quan-Yu Pan pin jia Ren-Yi Cao Zhi-Kai Wang Jun-Jie Shi College of Petroleum Engineering China University of Petroleum(Beijing)Beijing102249China Sinopec Petroleum Exploration and Production Research Institute Beijing100083China CNOOC Research Institute Beijing100028China State Key Laboratory of Water Resources Protection and Utilization in Coal Mining Beijing112211China

The loss of hydrocarbon production caused by the dynamic behavior of the inner boundary and propped fractures under long-term production conditions has been widely reported in recent ***,the quantitative relationships for the variations of the inner boundary and propped fractures have not been determined and incorporated in the semi-analytical models for the pressure and rate transient *** work focuses on describing the variations of the inner boundary and propped fractures and capturing the typical characteristics from the pressure transient curves.A generalized semi-analytical model was developed to characterize the dynamic behavior of the inner boundary and propped fractures under long-term production *** pressure-dependent length shrinkage coefficients,which quantify the length changes of the inner zone and propped fractures,are modified and incorporated into this multi-zone semi-analytical *** simultaneous numerical iterations and numerical inversions in Laplace and real-time space,the transient solutions to pressure and rate behavior are determined in just a few *** dynamic behavior of the inner boundary and propped fractures on transient pressure curves is divided into five periods:fracture bilinear flow(FR1),dynamic PFs flow(FR2),inner-area linear flow(FR3),dynamic inner boundary flow(FR4),and outer-area dominated linear flow(FR5).The early hump during FR2 period and a positive upward shift during FR4period are captured on the log-log pressure transient curves,reflecting the dynamic behavior of the inner boundary and propped fractures during the long-term production *** transient pressure behavior will exhibit greater positive upward trend and the flow rate will be lower with the shrinkage of the inner *** pressure derivative curve will be upward earlier as the inner boundary shrinks more *** lower permeability caused by the closure of un-propped fractures in the inner zone results in gr

关键词： Semi-analytical model Length shrinkage Dynamic behavior Boundary changes Transient behavior

维护与巩固文化领导权是马克思主义政党的重要工作

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毛泽东邓小平理论研究 2022年第7期 28-32,107页

作者：贾淑品上海师范大学马克思主义学院

新时代党的文化领导权主要面临文化虚无主义的挑战,反对文化虚无主义更要牢牢掌握文化领导权。文化领导权建设事关新时代中国特色社会主义文化建设和“文化现代化”的实现,在中国社会主义现代化建设进程中具有重要的战略地位和作用。历... 详细信息

新时代党的文化领导权主要面临文化虚无主义的挑战,反对文化虚无主义更要牢牢掌握文化领导权。文化领导权建设事关新时代中国特色社会主义文化建设和“文化现代化”的实现,在中国社会主义现代化建设进程中具有重要的战略地位和作用。历史上看,无产阶级执政党建设的一项重要工作就是争夺和巩固文化领导权,在这一过程中也形成了关于文化领导权的理论,这为我们有效应对文化虚无主义的挑战提供了参考。当前,我们应始终坚持党对文化的全面领导,以人民为中心,坚持党性与人民性相统一原则,用马克思主义筑起思想保障,更好抵御文化虚无主义侵蚀,不断巩固和提升党的文化领导权。

关键词：文化领导权马克思主义政党维护巩固

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列宁无产阶级文化领导权思想及其启示

毛泽东邓小平理论研究 2020年第10期 65-73,108页

作者：贾淑品上海师范大学马克思主义学院上海师范大学21世纪马克思主义研究中心

意识形态领域的斗争是争夺文化领导权的关键。列宁的文化领导权思想是在批判各种非马克思主义错误思潮的意识形态领域斗争过程中形成与确立的,其基础和核心内容就是坚持马克思主义理论的主导地位、坚持党的领导。当下,我们应当继承、发... 详细信息

意识形态领域的斗争是争夺文化领导权的关键。列宁的文化领导权思想是在批判各种非马克思主义错误思潮的意识形态领域斗争过程中形成与确立的,其基础和核心内容就是坚持马克思主义理论的主导地位、坚持党的领导。当下,我们应当继承、发展列宁的文化领导权思想,建设好、发挥好社会主义文化的凝聚力和引领力,高度重视意识形态工作,重视党的领导;掌握文化领导权,加快构建中国特色哲学社会科学;坚定文化自信,建设社会主义文化强国;在把握时代、创新方法中不断丰富和发展社会主义文化。

关键词：列宁无产阶级文化领导权启示

γ-羟基丁酸的五氟卞基溴衍生化气相色谱/质谱分析

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分析化学 2008年第1期36卷 61-65页

作者：孟品佳中国人民公安大学刑科技系北京100038

采用五氟卞基溴作为γ-羟基丁酸(GHB)的烷基化试剂,衍生化后进行了产物的净化处理,以降低衍生化产物对仪器的不利影响。考察了该衍生化反应的一些影响因素,优化了衍生化条件,并对衍生化产物进行了质谱解析。在此基础上考察了该方法的定... 详细信息

采用五氟卞基溴作为γ-羟基丁酸(GHB)的烷基化试剂,衍生化后进行了产物的净化处理,以降低衍生化产物对仪器的不利影响。考察了该衍生化反应的一些影响因素,优化了衍生化条件,并对衍生化产物进行了质谱解析。在此基础上考察了该方法的定量分析参数,结果表明,GHB在0.1～5mg/L的范围内,其衍生化产物具有良好的线性关系。在GHB浓度为1mg/L时,衍生化产物的相对标准偏差为13.4%。对添加尿样提取后进行衍生化并测试,检出限为10mg/L,与GHB的检测阈值相应。该研究建立了GHB分析的又一衍生化GC方法,可用于饮料中及生物样品中GHB的分析。

关键词： γ-羟基丁酸五氟卞基溴衍生化

Clinical characteristics and risk factors of severe respiratory syncytial virus-associated acute lower respiratory tract infections in hospitalized infants

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World Journal of Pediatrics 2014年第4期10卷 360-364页

作者： Xiao-Bo Zhang Li-Juan Liu Li-Ling Qian Gao-Li jiang Chuan-Kai Wang pin jia Peng Shi Jin Xu Li-Bo Wang Department of Respiratory Medicine Children's Hospital of Fudan University399 Wanyuan RoadShanghai 201102China

Background:To investigate the clinical characteristics and analyze risk factors for severe respiratory syncytial virus(RSV)infection in hospitalized infants with acute lower respiratory tract infections(ALRIs).Methods:A retrospective review of the medical records of infants with RSV-associated ALRIs between March 1st,2011 and February 29th,2012 was *** were followed up over the phone or by outpatient visit six and twelve months after ***:Among 913 RSV-associated ALRIs infants,288(31.5%)had severe infections,which accounted for 4.2%of hospitalized *** hospital RSV mortality rate was 1.0%.The proportions of cases with tachypnea,apnea,cyanosis,and fine rales were significantly higher in the severe ALRIs group(all P<0.001).Multivariate logistic regression showed that low-birth-weight[1.698(1.028-2.805)],age less than 3 months old[3.385(2.174-5.271)],congenital heart disease[1.667(1.149-2.418)],bronchopulmonary dysplasia[8.505(1.731-41.780)],and airway abnormalities[2.246(1.008-5.005)]were independent risk factors for severe *** incidence of bronchitis,pneumonia and readmission in the severe group was significantly higher than that of the non-severe group during the one-year follow-up(all P<0.001).Conclusions:Younger age,low birth weight and underlying disease are associated with severe RSVassociated ***,severe RSV infections may be associated with a higher frequency of subsequent bronchitis,pneumonia and re-hospitalization in the following year.

关键词： infant respiratory infectious diseases respiratory syncytial virus

基于岩体三轴压缩试验的节理力学参数确定方法

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岩土力学 2011年第11期32卷 3219-3224页

作者：王芝银段品佳中国石油大学城市油气输配技术北京市重点实验室北京102249

岩体节理面的刚度系数、黏结力和内摩擦角是反映节理面力学特性的重要参数。基于含不同倾角节理岩样的三轴压缩试验,通过对标准岩样的应力-位移分析,由互等功原理,建立节理岩样应变与位移新的几何关系,推导出弹性状态下节理刚度系数解析... 详细信息

岩体节理面的刚度系数、黏结力和内摩擦角是反映节理面力学特性的重要参数。基于含不同倾角节理岩样的三轴压缩试验,通过对标准岩样的应力-位移分析,由互等功原理,建立节理岩样应变与位移新的几何关系,推导出弹性状态下节理刚度系数解析解,根据摩尔-库仑强度理论得出节理面的黏结力和内摩擦角。为了检验方法的正确性,对含节理面的同类岩体进行直接剪切试验,测出节理面的法向刚度、切向刚度、黏结力和内摩擦角。结果表明,两种方法所得结果基本吻合,说明所提出的方法可以通过节理岩样三轴压缩试验及理论分析间接得到岩体节理面的刚度系数、黏结力和内摩擦角,实现了节理岩体力学参数的简捷、快速测定。

关键词：节理刚度系数强度指标互等功原理三轴压缩试验

Reasonable Range of Internal Pressure of Gas Storage in Rock Salt Mass

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Reasonable Range of Internal Pressure of Gas Storage in Rock...

2012年海峡两岸破坏科学/材料试验学术会议

作者： Wang Zhiyin Zhao Bin Duan pin jia Zhang huabin Beijing Key Laboratory of Urban Oil and Gas Distribution Technology China University of Petroleum(Beijing) CNOOC Gas & Power Group

Internal pressure has a controlling influence on the stability of cavern of gas storage in rock salt *** analytical formulas of the rock surrounding cavern,governing conditions and reasonable ranges of internal pressure under static and cycle load conditions for spherical and cylindrical cavern were obtained through mechanical analytical *** conditions were based on tensile failure condition,and plasticity model including Mohr-Coulomb criterion and Drucker-Prager *** results indicated that reasonable internal pressure ranges can prevent a shear or tensile failure of the surrounding rocks,and the internal pressure ranges based on Drucker-Prager criterion are more usable in practical engineering of salt cavern gas storage,because it takes intermediate principal stress into account and its characteristics of three dimensional stress state is consistent with the real force sate of surrounding rocks of cavern.

关键词： Gas Storage in Rock Salt Mass Internal Pressure Variation Range Analytic Method

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皖北贫困地区农村社会保障存在的问题及对策

安徽农业科学 2007年第36期35卷 12079-12081页

作者：贾淑品淮南师范学院政法系安徽淮南232001

构建和谐社会,统筹城乡经济社会发展的重要一环是建立新型农村社会保障体系,而皖北贫困地区农村社会保障更是其中薄弱的一环,其解决的好坏,将直接影响到新农村建设乃至整个社会的持续发展及和谐社会的构建。针对当今皖北贫困地区农村社... 详细信息

构建和谐社会,统筹城乡经济社会发展的重要一环是建立新型农村社会保障体系,而皖北贫困地区农村社会保障更是其中薄弱的一环,其解决的好坏,将直接影响到新农村建设乃至整个社会的持续发展及和谐社会的构建。针对当今皖北贫困地区农村社会保障的现实背景、存在问题及已有研究理论进行仔细分析后,提出了解决这一问题的具体措施,主要包括:建立和完善农村社会救济制度、健全农村医疗保障制度等,以构建切实有效的农村社会保障模式。

关键词：贫困地区农村社会保障存在的问题对策