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Phylogenetic and Structural Analysis of Major Surface Proteins Hemagglutinin and Neuraminidase of Novel Avian Influenza Virus A H7N9 from Chinese Patient

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Chemical Research in Chinese Universities 2013年第5期29卷 934-940页

作者： YUAN Xiao-hui WANG Ying-chen QU Zhang-yi ren jia-yi WU Xiao-min WANG Jing-fei Department of Microbiology Public Health College Harbin Medical University Harbin 150081 P. R. China Institute of Pathogen Biology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100176 P R. China Key Laboratory of Resource and Plant Biology of Anhui Province College of Life Science Huaibei Normal University Huaibei 235000 P. R. China Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150081 P R. China

This research reveals the phylogenetic history and structural information of the hemagglutinin（HA） and neuraminidase（NA） from novel avian influenza virus A/Hangzhou/1/2013（HTN9_2013） strain from human infected in China. Strains closely related to the HTN9 2013 strain were obtained from Nation Center for Biotechnology Information（USA）-basic local alignment search tool（NCBI-BLAST） searching, and the phylogenetic trees were con- structed. The 3D structures of HA and NA from H7N9 2013 strain were built by homology modeling technology, and molecular dynamics（MD） simulations were performed on the high-performance computer cluster. Characteristic amino acid sites were then screened from multiple sequence alignment（MSA） via home-made Python script and mapped onto the 3D structures. The thermodynamic characteristic root-mean-square-fluctuation （RMSF） of these sites in the structure was also analyzed with MD trajectories. The HA of HTN9_2013 strain is closely related to the A/duck/Zhejiang/12/2011 strain isolated in China, while the NA of HTN9 2013 strain is mostly related to the A/mallard/Czech Republic/13438-29K/2010 strain isolated in Europe. The 3D structures of HA and NA from H7N9 2013 stain are mostly identical to the existing structure of H7 and N9. A total of 11 and 14 characteristic ami- no acid sites were identified in HA and NA, respectively, in HTN9_2013. Structural analysis indicates that certain sites in the top region of HA are important, at which the mutation of some amino acids can impact the receptor bin- ding that may be related to its infection of human beings.

关键词： Avian influenza virus Hemagglutinin Neuraminidase Homology modeling Phylogenetic

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Expression of midkine and its clinical significance in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2006年第13期12卷 2006-2010页

作者： Ying-jia ren Qing-Yun Zhang Department of Immunology The School of Ontology Peking University Beijing Institute for Cancer Research Beijing 100036 China Department of Medical Laboratory Beijing Cancer Hospital Beijing 100036 China

AIM： To investigate the expression of midkine in eso- phageal squamous cell carcinoma （ESCC） and analyze its relationship with clinicopathological features. METHODS： RT-PCR and immunocytochemical staining were used to detect the expression of midkine mRNA and protein in EC109 cells, respectively. Then the expression of midkine in 66 cases of ESCC samples were detected by immunohistochemistry using monoclonal antibodies against human midkine. RESULTS： Midkine was expressed in EC109 cell by RT- PCR and imrnunocytochemistry. The immunoreactivity was detected in 56.1% （37/66） of the ESCC samples. The expression of midkine was found in cytoplasm of tumor cells. Notably, the intensity of midkine was stronger at the area abundant in vessels and the invading border of the tumors. Midkine was more intensely expressed in well differentiated tumors （76.9 %） than in moderately and poorly differentiated tumors （43.1% and 41.2%, respectively）（P〈0.05）. There was no statistically significant correlation between midkine expression and gender, age, clinical stage, lymph node metastasis or survival in ESCC. CONCLUSION： IVlidkine is overexpressed in ESCC. It may play a role in tumor angiogenesis and invasion. The expression of midkine is correlated with tumor cell differentiation in ESCC. The more poorly tumor cells differentiate, the weaker midkine expresses.

关键词： Midkine Esophageal squamous cell carcinoma Expression

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LIPUS Enhance Elongation of Neurites in Rat Cortical Neurons through Inhibition of GSK-3β

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Biomedical and Environmental Sciences 2010年第3期23卷 244-249页

作者： CONG ren jia-MOU LI XIN LIN Department of Orthopedics Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Objective Low-intensity pulsed ultrasound （LIPUS） has been reported to enhance proliferation and to alter protein production in various kinds of cells. In the present study, we measured the neurites length after LIPUS treatment to define the effectiveness of LIPUS stimulation on neurons, and then we examined the acticity of GSK-3β to study the intracellular mechanism of neurite＇s outgrowth. Methods LIPUS was applied to cultured primary rat cortical neurons for 5 minutes every day with spatial- and temporal average intensities （SATA） of 10 mW/cm^2, a pulse width of 200 microseconds, a repetition rate of 1.5 KHz, and an operation frequency of 1 MHz. Neurons were photographed on the third day after LIPUS treatment and harvested at third, seventh, and tenth days for immnoblot and semi-quantitative RT-PCR analysis. Results Morphology change showed that neurite extension was enhanced by LIPUS. There was also a remarkable decrease of proteins, including p-Akt, p-GSK-3β, and p-CRMP-2, observed on the seventh and tenth days, and of GSK-3β mRNA expression, observed on the seventh day, in neurons treated with LIPUS. Conclusion LIPUS can enhance elongation of neurites and it is possible through the decreased expression of GSK-3β.

关键词： LIPUS Rat cortical neurons GSK-3β

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Effects of angiotensin-converting enzyme inhibitor and angiotensin Ⅱ receptor blocker on one-year outcomes of patients with atrial fibrillation: insights from a multicenter registry study in China

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Journal of Geriatric Cardiology 2020年第12期17卷 750-758页

作者： Si-Qi LYU Yan-Min YANG Jun ZHU Juan WANG Shuang WU jia-Meng ren Han ZHANG Xing-Hui SHAO Emergency and Critical Care Center State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Objective To evaluate the effect of angiotensin-converting enzyme inhibitor(ACEI)/angiotensin Ⅱ receptor blocker(ARB)therapy on the prognosis of patients with atrial fibrillation(AF).Methods A total of 1,991 AF patients from the AF registry were divided into two groups according to whether they were treated with ACEI/ARB at *** characteristics were carefully collected and *** regression was utilized to identify the predictors of ACEI/ARB *** primary endpoint was all-cause mortality,while the secondary endpoints included cardiovascular mortality,stroke and major adverse events(MAEs)during the one-year follow-up *** and multivariable Cox regression were performed to identify the association between ACEI/ARB therapy and the one-year *** In total,759 AF patients(38.1%)were treated with ACEI/*** with AF patients without ACEI/ARB therapy,patients treated with ACEI/ARB tended to be older and had a higher rate of permanent AF,hypertension,diabetes mellitus,heart failure(HF),left ventricular ejection fraction(LVEF)<40%,coronary artery disease(CAD),prior myocardial infarction(MI),left ventricular hypertrophy,tobacco use and concomitant medications(all P<0.05).Hypertension,HF,LVEF<40%,CAD,prior MI and tobacco use were determined to be predictors of ACEI/ARB *** analysis showed that ACEI/ARB therapy was associated with a significantly lower risk of one-year all-cause mortality[hazard ratio(HR)(95%CI):0.682(0.527-0.882),P=0.003],cardiovascular mortality[HR(95%CI):0.713(0.514-0.988),P=0.042]and MAEs[HR(95%CI):0.698(0.568-0.859),P=0.001].The association between ACEI/ARB therapy and reduced mortality was consistent in the subgroup *** In patients with AF,ACEI/ARB was related to significantly reduced one-year all-cause mortality,cardiovascular mortality and MAEs despite the high burden of cardiovascular comorbidities.

关键词： Angiotensin-converting enzyme inhibitor Angiotensin II receptor blocker Atrial fibrillation Mortality

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Morphology and crystal-plane effects of Zr-doped CeO2 nanocrystals on the epoxidation of styrene with tert-butylhydroperoxide as the oxidant

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Journal of Energy Chemistry 2017年第4期26卷 681-687页

作者： jia ren Xin Liu Ruihua Gao Wei-Lin Dai Department of Chemistry&Shanghai Key Laboratory of Molecular Catalysis and Innovative Material Fudan UniversityShanghai 200433China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200433China

The morphology effect of Zr-doped CeOwas studied in terms of their activities in the selective oxidation of styrene to styrene oxide using tert-butyl hydroperoxide as the oxidant. In the present work, Zrdoped CeOnanorods exhibited the highest catalytic performance（yield of styrene oxide and TOF value）followed by nanoparticles and nanocubes. For the Zr-doped CeOnanorods, the apparent activation energy is 56.3 k J/mol, which is much lower than the values of catalysts supported on nanoparticles and nanocubes（73.3 and 93.4 k J/mol）. The high resolution transmission electron microscopy results indicated that（100） and（110） crystal planes are predominantly exposed for Zr-doped CeOnanorods while（100）and（111） for nanocubes,（111） for nanoparticles. The remarkably increased catalytic activity of the Zrdoped CeOnanorods is mainly attributed to the higher percentage of Cespecies and more oxygen vacancies, which are associated with their exposed（100） and（110） crystal planes. Furthermore, recycling studies proved that the heterogeneous Zr-doped CeOnanorods did not lose its initial high catalytic activity after five successive recycles.

关键词： Morphology effect Zr-doped CeO2 Styrene Selective oxidation Oxygen vacancies

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Long noncoding RNAs in gastric cancer: From molecular dissection to clinical application

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World Journal of Gastroenterology 2020年第24期26卷 3401-3412页

作者： Yue Gao Jun-Wei Wang jia-Yi ren Mian Guo Cheng-Wang Guo Shang-Wei Ning Shan Yu College of Bioinformatics Science and Technology Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina Department of Respiratory Medicine The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina Department of Neurosurgery the Second Affiliated Hospital of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina Department of Gastroenterology and Gastrosurgery Gansu Wuwei Tumor HospitalWuwei 733000Gansu ProvinceChina Department of Pathology the Second Affiliated Hospital of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina

Long noncoding RNAs(lncRNAs)are important regulators of cell processes that are usually dysregulated in gastric cancer(GC).Based on their high specificity and ease of detection in tissues and body fluids,increasing attention has spurred the study of the roles of lncRNAs in GC ***,it is necessary to elucidate the molecular mechanisms and further explore the clinical applications of lncRNAs in *** this review,we summarize current knowledge to examine dysregulated lncRNAs in GC and their underlying molecular mechanisms and activities in GC,which involve microRNA sponging,mRNA stability,genetic variants,alternative splicing,transcription factor binding,and epigenetic *** significantly,the potential of lncRNAs as prognostic,circulating,and drug-resistant biomarkers for GC is also *** review highlights the method of dissecting molecular mechanisms to explore the clinical application of lncRNAs in ***,this review offers assistance in using lncRNAs as novel candidates for molecular mechanisms and for the identification of revolutionary biomarkers for GC.

关键词： Gastric cancer Long noncoding RNAs Molecular mechanism Biomarkers Therapeutic target Drug resistance

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MUC1-positive circulating tumor cells and MUC1 protein predict chemotherapeutic efficacy in the treatment of metastatic breast cancer

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Chinese Journal of Cancer 2011年第1期30卷 54-61页

作者： jian-Ping Cheng Ying Yan Xiang-Yi Wang Yuan-Li Lu Yan-Hua Yuan Jun jia Jun ren Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Peking University School of Oncology Department of Medical Oncology Beijing Cancer Hospital & Institute Beijing 100142 P. R. China.

Chemotherapy plays an important role in the treatment of metastatic breast cancer. It is important to monitor chemotherapeutic efficacy, to find a simple and efficient tool to guide treatment, and to predict the efficacy of treatment in a timely and accurate manner. This study aimed to detect mucin-1 (MUC1) - positive circulating tumor cells and MUC1 protein in the peripheral blood of patients with metastatic breast cancer and to investigate their relationship to chemotherapeutic efficacy. MUC1 mRNA was detected in the peripheral blood of 34 patients with newly diagnosed metastatic breast cancer by reverse transcription- polymerase chain reaction. The positive rates of MUC1 mRNA were 88.2% before chemotherapy and 70.6% after chemotherapy, without a significant difference (P = 0.564); MUC1 mRNA expression before chemotherapy had no correlation with treatment effectiveness (P = 0.281). The response rate of MUC1 mRNA -negative patients after first-cycle chemotherapy was significantly higher (P = 0.009) and the progression-free survival (PFS) was clearly longer than those of MUC1 mRNA-positive patients (P = 0.095). MUC1 protein in peripheral blood plasma was detected by an ELISA competitive inhibition assay. The patients with decreased MUC1 protein after chemotherapy had a significantly longer PFS than those with elevated MUC1 protein (P = 0.044). These results indicate that the outcomes of MUC1 mRNA - negative patients after chemotherapy are better than those of MUC1 mRNA-positive patients. In addition, patients with decreased expression of MUC1 protein have a better PFS.

关键词： MUC1 治疗效果肿瘤细胞阳性率乳腺癌转移性粘蛋白化疗

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Seeing Dirac electrons and heavy fermions in new boron nitride monolayers

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Chinese Physics B 2020年第5期29卷 454-458页

作者： Yu-jiao Kang Yuan-Ping Chen jia-ren Yuan Xiao-Hong Yan Yue-E Xie School of Physics and Optoelectronics Xiangtan UniversityXiangtan 411105China Faculty of Science Jiangsu UniversityZhenjiang 212013China

Most three-dimensional(3D)and two-dimensional(2D)boron nitride(BN)structures are wide-band-gap ***,we propose two BN monolayers having Dirac points and flat bands,*** monolayer is named as 5-7 BN that consists of five-and seven-membered *** other is a Kagome BN made of triangular boron rings and nitrogen *** two structures show not only good dynamic and thermodynamic stabilities but also novel electronic *** 5-7 BN has Dirac points on the Fermi level,indicating that the structure is a typical Dirac *** Kagome BN has double flat bands just below the Fermi level,and thus there are heavy fermions in the *** flat-band-induced ferromagnetism is also *** analyze the origination of the band structures by partial density of states and projection of *** addition,a possible route to experimentally grow the two structures on some suitable substrates such as the PbO2(111)surface and the CdO(111)surface is also discussed,*** research not only extends understanding on the electronic properties of BN structures,but also may expand the applications of BN materials in 2D electronic devices.

关键词： boron nitride Dirac fermion flat band ferromagnetism

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Reliability study of custom designed ADC for the jiangmen underground neutrino observatory

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Radiation Detection Technology and Methods 2020年第2期4卷 203-207页

作者： Ru-yi Jin Jun Hu jia-yi ren Zhe Ning Xiao-shan jiang State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of Sciences(CAS)Beijing 100049China University of Chinese Academy of Sciences(UCAS) Beijing 100049China

Objective The jiangmen underground neutrino observatory(JUNO)is the largest and most precise liquid scintillator detector under construction in the south of *** front-end readout electronics system will be installed very close to the photomultiplier tubes under ***,the system’s absolute reliability is *** an important piece,the failure rate of the custom designed analog-to-digital convertor(ADC)must be *** The Arrhenius High Temperature Operating Life(HTOL)model is usually used to calculate the standard reliability value failure rate(λ).A temperature acceleration test of the ADC is performed with a 115℃ambient temperature based on a custom aging *** performance of ADC is compared before and after aging,and the most significant bit(MSB)of the digital output is monitored during the *** The result of a 2400-hour-long test shows that there were no *** upper limit of the failure rate is calculated as 5.8FIT,which can be added to the failure rate of other components to meet the overall failure rate requirements(84FIT).Conclusion With the test system,we established a method based on the HTOL model to obtain the upper limit of the ADC reliability for overall failure rate calculation in *** method can be applied to the reliability measurement for any other custom designed *** experiment can be repeated with a new set of parameters to get a satisfactory failure rate according to other overall system requirements.

关键词： ADC Reliability HTOL Failure rate

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电动汽车动力电池区块链技术的投资演化博弈

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同济大学学报（自然科学版） 2023年第5期51卷 660-666,F0002页

作者：尤建新任佳同济大学经济与管理学院上海200092

为促进汽车生产商积极主动投资区块链技术,基于演化博弈理论,构建电池生产商、整车生产商和政府的三方演化博弈模型,探讨政府参与下汽车生产商区块链技术投资行为,分析三类参与主体之间的行为选择及演化均衡状态,并通过仿真模拟区块链... 详细信息

为促进汽车生产商积极主动投资区块链技术,基于演化博弈理论,构建电池生产商、整车生产商和政府的三方演化博弈模型,探讨政府参与下汽车生产商区块链技术投资行为,分析三类参与主体之间的行为选择及演化均衡状态,并通过仿真模拟区块链技术特征参数和政府参与行为对于系统稳态的影响。研究表明汽车生产商在区块链技术投资系统中的策略选择与区块链技术所带来的额外收益有关;区块链技术带来的优势、政府所提供的间接收益率都能促进区块链技术投资系统较快地趋向理想均衡状态,而技术引进奖励的波动则对这一速度影响较小。研究结果可以为促进区块链技术引入到动力电池回收行业提供一定的理论指导。

关键词：动力电池区块链技术投资演化博弈

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