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Role of ACLY in the development of gastric cancer under hyperglycemic conditions

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Quantitative Biology 2024年第1期12卷 100-116页

作者： Keran Sun Jingyuan Ning Keqi jia xiaoqing Fan Hongru Li Jize Ma Meiqi Meng Cuiqing Ma Lin Wei Department of Immunology Immunology Department of Hebei Medical UniversityShijiazhuangChina Department of Pathology Pathology Department of the Fourth Hospital of Hebei Medical UniversityShijiazhuangChina

To investigate the impact of hyperglycemia on the prognosis of patients with gastric cancer and identify key molecules associated with high glucose levels in gastric cancer development,RNA sequencing data and clinical features of gastric cancer patients were obtained from The Cancer Genome Atlas(TCGA)database.High glucose-related genes strongly associated with gastric cancer were identified using weighted gene co-expression network and differential analyses.A gastric cancer prognosis signature was constructed based on these genes and patients were categorized into high-and low-risk groups.The immune statuses of the two patient groups were compared.ATP citrate lyase(ACLY),a gene significantly related to the prognosis,was found to be upregulated upon high-glucose stimulation.Immunohistochemistry and molecular analyses confirmed high ACLY expression in gastric cancer tissues and cells.Gene Set Enrichment Analysis(GSEA)revealed the involvement of ACLY in cell cycle and DNA replication processes.Inhibition of ACLY affected the proliferation and migration of gastric cancer cells induced by high glucose levels.These findings suggest that ACLY,as a high glucose-related gene,plays a critical role in gastric cancer progression.

关键词： ACLY gastric cancer high glucose immune microenvironment

The fast method and convergence analysis of the fractional magnetohydrodynamic coupled flow and heat transfer model for the generalized second-grade fluid

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Science China Mathematics 2024年第4期67卷 919-950页

作者： xiaoqing Chi Hui Zhang Xiaoyun jiang School of Mathematics and Statistics Shandong UniversityWeihai 264209China School of Mathematics Shandong UniversityJinan 250100China

In this paper,we first establish a new fractional magnetohydrodynamic(MHD)coupled flow and heat transfer model for a generalized second-grade fluid.This coupled model consists of a fractional momentum equation and a heat conduction equation with a generalized form of Fourier law.The second-order fractional backward difference formula is applied to the temporal discretization and the Legendre spectral method is used for the spatial discretization.The fully discrete scheme is proved to be stable and convergent with an accuracy of O(τ^(2)+N-r),whereτis the time step-size and N is the polynomial degree.To reduce the memory requirements and computational cost,a fast method is developed,which is based on a globally uniform approximation of the trapezoidal rule for integrals on the real line.The strict convergence of the numerical scheme with this fast method is proved.We present the results of several numerical experiments to verify the effectiveness of the proposed method.Finally,we simulate the unsteady fractional MHD flow and heat transfer of the generalized second-grade fluid through a porous medium.The effects of the relevant parameters on the velocity and temperature are presented and analyzed in detail.

关键词： fractional MHD coupled flow and heat transfer model generalized second-grade fuid fast method convergence analysis numerical simulation

Physiochemical and biological characteristics of fouling on landfill leachate treatment systems surface

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Journal of Environmental Sciences 2024年第1期135卷 59-71页

作者： Zhourui Gong Shu Yang Rui Zhang Yangqing Wang xiaoqing Wu Liyan Song School of resources and environmental engineering Anhui UniversityHefei 230601China Anhui Shengjin Lake Wetland Ecology National Long-term Scientific Research Base Dongzhi 247230China Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment Ministry of EducationChongqing UniversityChongqing 400045China Chongqing Institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714China Xing Lu Huan Jing Co.LTD. Luzhou 646000China

Fouling of landfill leachate,a biofilm formation process on the surface of the collection system,migration pipeline and treatment system causes low efficiency of leachate transportation and treatment and increases cost for maintenance of those facilities.In addition,landfill leachate fouling might accumulate pathogens and antibiotic resistance genes(ARGs),posing threats to the environment.Characterization of the landfill leachate fouling and its associated environmental behavior is essential for the management of fouling.In this study,physicochemical and biological properties of landfill leachate fouling and the possible accumulation capacity of pathogens and ARGs were investigated in nitrification(aerobic condition)and denitrification(anaerobic condition)process during landfill leachate biological treatment,respectively.Results show that microbial(bacterial,archaeal,eukaryotic,and viral)community structure and function(carbon fixation,methanogenesis,nitrification and denitrification)differed in fouling under aerobic and anaerobic conditions,driven by the supplemental leachate water quality.Aerobic fouling had a higher abundance of nitrification and denitrification functional genes,while anaerobic fouling harbored a higher abundance of carbon fixation and methanogenesis genes.Both forms of leachate fouling had a higher abundance of pathogens and ARGs than the associated leachate,suggesting the accumulation capacity of fouling on biotic pollutants.Specifically,aerobic fouling harbored three orders of magnitude higher multidrug resistance genes mex D than its associated leachate.This finding provides fundamental knowledge on the biological properties of leachate fouling and suggests that leachate fouling might harbor significant pathogens and ARGs.

关键词： Landfill leachate fouling Biofilm Microbiome Carbon and nitrogen cycle Pathogens Antibiotic resistance genes

Engineering particles towards 3D supraballs-based passive cooling via grafting CDs onto colloidal photonic crystals

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Chinese Chemical Letters 2024年第4期35卷 457-463页

作者： Jie Wu xiaoqing Yu Guoxing Li Su Chen State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing 210009China

Particle engineering has opened the floodgates to material science in both fundamental and application field. However, covalent interactions have not yet been adequately designed in the particle engineering for functional colloidal photonic crystals(CPCs). Herein, we achieved covalent coupling between carboxylrich poly(styrene-acrylic acid)(P(St-AA)) monodispersed colloidal particles and amine-rich carbon dots(CDs) based on an feasible and universal particle engineering strategy. The designed CDs-grafted P(St-AA)monodispersed colloidal particles initiate a hydrogen bond-driven assembly mode and ensure the construction of large-scale crack-free CPCs. Moreover, the CDs equipped with selective broad-band absorption capacity could improve the saturation of structural colors for high-visibility CPCs. Furthermore, an injectable photonic hydrogel(IPH) is developed to design CPC supraball hydrogel via integrating the CDsgrafted P(St-AA) CPC supraballs with supramolecular hydrogel. Combining superior flexibility, sufficient self-healing capacity of supramolecular hydrogel with visual optical information of our CPC supraballs, a cyclically reversible coding and decoding system was developed. Meanwhile, we firstly demonstrated the novel strategy of 3D supraballs-based passive cooling. The designed 3D CPC supraball hydrogel presents nearly full observation angle reflections behavior and excellent water evaporation capacity and achieves3.6 ℃ temperature drops, showing the application advantages in 3D thermal management. This work not only provides a new insight for manipulating optical properties of CPCs, but also demonstrates an easyto-perform platform, as well as indicates the direction for the promising application of CPCs.

关键词： Particle engineering Carbon dots Colloidal photonic crystals Passive cooling Digital coding

Non-uniform thermal behavior of single-layer spherical reticulated shell structures considering time-variant environmental factors: analysis and design

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第3期25卷 223-237页

作者： Wucheng XU xiaoqing ZHENG Xuanhe ZHANG Zhejie LAI Yanbin SHEN College of Civil Engineering and Architecture Zhejiang UniversityHangzhou 310058China Center for Balance Architecture Zhejiang UniversityHangzhou 310028China Innovation Center of Yangtze River Delta Zhejiang UniversityJiaxing 314100China Zhejiang Provincial Key Laboratory of Space Structures Zhejiang UniversityHangzhou 310058China

Contrary to conventional design methods that assume uniform and slow temperature changes tied to atmospheric conditions,single-layer spherical reticulated shells undergo significant non-uniform and time-variant temperature variations due to dynamic environmental coupling.These differences can affect structural performance and pose safety risks.Here,a systematic numerical method was developed and applied to simulate long-term temperature variations in such a structure under real environmental conditions,revealing its non-uniform distribution characteristics and time-variant regularity.A simplified design method for non-uniform thermal loads,accounting for time-variant environmental factors,was theoretically derived and validated through experiments and simulations.The maximum deviation and mean error rate between calculated and tested results were 6.1℃ and 3.7%,respectively.Calculated temperature fields aligned with simulated ones,with deviations under 6.0℃.Using the design method,non-uniform thermal effects of the structure are analyzed.Maximum member stress and nodal displacement under non-uniform thermal loads reached 119.3 MPa and 19.7 mm,representing increases of 167.5%and 169.9%,respectively,compared to uniform thermal loads.The impacts of healing construction time on non-uniform thermal effects were evaluated,resulting in construction recommendations.The methodologies and conclusions presented here can serve as valuable references for the thermal design,construction,and control of single-layer spherical reticulated shells or similar structures.

关键词： Non-uniform temperature field Non-uniform thermal load Non-uniform thermal effect Single-layer spherical reticulated shell Time-variant environmental factor

Gut microbiota intervention attenuates thermogenesis in broilers exposed to high temperature through modulation of the hypothalamic 5‑HT pathway

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Journal of Animal Science and Biotechnology 2024年第2期15卷 679-698页

作者： Sheng Li xiaoqing Li Kai Wang Yansen Li Kentaro Nagaoka Chunmei Li Research Centre for Livestock Environmental Control and Smart Production College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Laboratory of Veterinary Physiology Department of Veterinary MedicineFaculty of AgricultureTokyo University of Agriculture and TechnologyTokyo 183‑8509Japan

Background Broilers have a robust metabolism and high body temperature,which make them less tolerant to hightemperature(HT)environments and more susceptible to challenges from elevated temperatures.Gut microbes,functioning as symbionts within the host,possess the capacity to significantly regulate the physiological functions and environmental adaptability of the host.This study aims to investigate the effects of gut microbial intervention on the body temperature and thermogenesis of broilers at different ambient temperatures,as well as the underlying mechanism involving the"gut-brain"axis.Methods Broilers were subjected to gut microbiota interference with or without antibiotics(control or ABX)starting at 1 day of age.At 21 day of age,they were divided into 4 groups and exposed to different environments for 7 d:The control and ABX groups at room temperature(RT,24±1℃,60%relative humidity(RH),24 h/d)and the control-HT and ABX-HT groups at high temperature(HT,32±1℃,60%RH,24 h/d).Results The results demonstrated that the antibiotic-induced gut microbiota intervention increased body weight and improved feed conversion in broiler chickens(P<0.05).Under HT conditions,the microbiota intervention reduced the rectal temperature of broiler chickens(P<0.05),inhibited the expression of avUCP and thermogenesisrelated genes in breast muscle and liver(P<0.05),and thus decreased thermogenesis capacity.Furthermore,the gut microbiota intervention blunted the hypothalamic‒pituitary‒adrenal axis and hypothalamic–pituitary–thyroid axis activation induced by HT conditions.By analyzing the cecal microbiota composition of control and ABX chickens maintained under HT conditions,we found that Alistipes was enriched in control chickens.In contrast,antibioticinduced gut microbiota intervention resulted in a decrease in the relative abundance of Alistipes(P<0.05).Moreover,this difference was accompanied by increased hypothalamic 5-hydroxytryptamine(5-HT)content and TPH2 expression(P<0.05).Conclusions These findings underscore the critical role

关键词： 5-HT Broiler chickens Gut microbiota Thermogenesis Thermoregulation

A Label-Free Deoxyribozyme Sensor for m^(6)A Demethylase Activity Detection

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Chinese Journal of Chemistry 2024年第9期42卷 951-956页

作者： Xiuyuan Wang Shusheng Yu Yuqiu He Fuan Wang xiaoqing Liu College of Chemistry and Molecular Sciences Wuhan UniversityWuhanHubei 430072China

A label-free deoxyribozyme(DNAzyme)biosensor for m^(6) demethylase activity detection is developed.When demethylase FTO(Fat mass and obesity-associated protein),an important m^(6) demethylase of ALKBH demethylase family is present,the m^(6) group is specifically demethylated,and the cleavage activity of DNAzyme is restored.This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T(ThT)and generate fluorescence signals.The biosensor shows high specificity and sensitivity,and fast reaction speed.Our study demonstrates a new design of allosteric DNAzyme for sensing.This method represents the first label-free nucleic acid biosensor for FTO assay,providing a feasible route towards inhibitors screening.

关键词： DNAzyme Analytical methods Biosensing m^(6)A demethylation Fluorescence

Commercial genetically modified corn and soybean are poised following pilot planting in China

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Molecular Plant 2024年第4期17卷 519-521页

作者： Mei Sun Suzhen Li Wenzhu Yang Bowen Zhao Youhua Wang xiaoqing Liu Biotechnology Research Institute Chinese Academy of Agricultural ScienceBeijing 100081China Farmers'Daily Beijing 100025China

"Who will feed China?"This was a question raised by Lester Brown,the director of the World Watch Institute in the United States,in 1994.He predicted that China's grain production would decrease from 340 million tons in 1990 to 272 million tons by 2030,a 20%decrease,while China's population would reach 1.6 billion(Brown,1995).If the dietary structure was not improved,China would need to import 200-369 million tons of grain,equivalent to the global grain trade at the time.He projected the high possibility of a food crisis in China,which might further trigger a global food crisis.However,with the joint efforts of national agricultural researchers,represented by Yuan Longping,and the Chinese government,China's grain production has achieved 18 consecutive increases from 2004 to 2021.In 2022,grain production in China reached 633 million tons(NBS and Statistics,2022),while the population remained stable at 1.4 billion.As a result,China has achieved complete self-sufficiency in its staple food supply and has demonstrated to the international community through practical actions that the Chinese people can feed themselves.

关键词： planting soybean raised

Surface engineering of carbon dots for highly sensitive α-glucosidase assay and inhibition evaluation

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Chinese Chemical Letters 2024年第3期35卷 193-197页

作者： Meijuan Liang Gege Song Yeqing Wan Yingying Chen Fuan Wang xiaoqing Liu College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Oil Crops Research Institute Chinese Academy of Agricultural SciencesWuhan 430062China

Monitoringα-glucosidase(α-Glu)activity is of great significance for the early diagnosis of typeⅡdiabetes.Here the blue fluorescent carbon dots(CDs)were integrated with two different recognizing molecules,β-cyclodextrin and phenylboronic acid,for assembling a multifunctional CDs(mCDs)nanoplatform for sensitively analyzingα-Glu and its inhibitors.The hydrolyzed product of 4-nitrophenyl-α-D-glucopyranoside(α-Glu substrate),p-nitrophenol,could efficiently quench the fluorescence of mCDs due to its cooperative molecular recognition withβ-cyclodextrin and phenylboronic acid.The mCDs could be utilized for the detection ofα-Glu activity with the limit of detection of 0.030 U/L.Moreover,the presentα-Glu detection platform revealed a high selectivity,and other natural enzymes showed scarcely any effect on the present mCDs system.The proposed method could be facilely used to screenα-Glu inhibitors with satisfying performance.The rational mCDs is expected to supplement more comprehensive biosensing platforms for highly sensitive and specific recognition of disease-relevant biomarkers with clinical importance.

关键词： α-Glucosidase Carbon dots Fluorescent sensor Inhibitor β-Cyclodextrin