A Label-Free Deoxyribozyme Sensor for m^(6)A Demethylase Activity Detection

作     者：Xiuyuan Wang Shusheng Yu Yuqiu He Fuan Wang Xiaoqing Liu Xiuyuan Wang;Shusheng Yu;Yuqiu He;Fuan Wang;Xiaoqing Liu

作者机构：College of Chemistry and Molecular SciencesWuhan UniversityWuhanHubei 430072China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2024年第42卷第9期

页      面：951-956页

核心收录：

学科分类：080202[工学-机械电子工程] 08[工学] 0802[工学-机械工程] 

基　　金：supported by the Natural Science Foundation of Jiangsu Province(BK20221258) the National Natural Science Foundation of China(22274121 and 22274123).We thank Prof.Xiang Zhou at Wuhan University for CD measurement 

主　　题：DNAzyme Analytical methods Biosensing m^(6)A demethylation Fluorescence 

摘      要：A label-free deoxyribozyme(DNAzyme)biosensor for m^(6) demethylase activity detection is *** demethylase FTO(Fat mass and obesity-associated protein),an important m^(6) demethylase of ALKBH demethylase family is present,the m^(6) group is specifically demethylated,and the cleavage activity of DNAzyme is *** allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T(ThT)and generate fluorescence *** biosensor shows high specificity and sensitivity,and fast reaction *** study demonstrates a new design of allosteric DNAzyme for *** method represents the first label-free nucleic acid biosensor for FTO assay,providing a feasible route towards inhibitors screening.