检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

应急救治呼吸机评价筛选指标研究

医疗卫生装备 2024年第7期45卷 8-16页

作者：姚勤岐唐明康叶龙莹张培培王科盛凌丹何倩鸿陈柱电子科技大学机械与电气工程学院成都611731 四川大学华西医院医学工程科成都610041

目的:为解决应急救治呼吸机可靠性评价筛选指标缺乏的问题,提出一种应急救治呼吸机评价筛选指标。方法:首先利用回归模型对呼吸机临床使用数据进行清洗,去除数据中的噪声以保证回归分析的精确度;然后基于曲线拟合和传递函数极点个数等... 详细信息

目的:为解决应急救治呼吸机可靠性评价筛选指标缺乏的问题,提出一种应急救治呼吸机评价筛选指标。方法:首先利用回归模型对呼吸机临床使用数据进行清洗,去除数据中的噪声以保证回归分析的精确度;然后基于曲线拟合和传递函数极点个数等方法确定吸气潮气量-患者分钟呼气量、峰值气道压力-患者分钟呼气量、峰值气道压力-吸气潮气量和PEEP(呼气末正压)-平均气道压力4组具有很强相关性的数据组合,并将4组数据组合理论输出和实际输出的差值作为判断呼吸机工作是否正常的指标;最后将该指标应用于单台和多台呼吸机,通过正常工作的呼吸机比例来判断提出指标的可行性。结果:单台呼吸机评价结果表明,4组数据组合输出的实际值和理论值拟合程度均较高,且4组数据组合输出差值均小于阈值;多台呼吸机评价结果表明,所有呼吸机中正常工作的比例为71.49%,与医院实际呼吸机正常工作的比例(72%)非常接近。结论:提出的应急救治呼吸机评价筛选指标具有可行性,可为应急救治系列设备的可靠性评价和筛选提供理论支撑。

关键词：呼吸机可靠性评价呼吸机评价呼吸机筛选应急救治设备

Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R

在线全文

学校读者我要写书评

暂无评论

mLife 2022年第3期1卷 329-340页

作者： Pengfei zhu Lihui Ren Ying zhu jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng Rongze Chen Xiaoting Fu Lili Zhang Lijun Sun Yuanqi zhu Yuetong ji Bo Ma Yingchun Xu jian Xu Qiwen Yang Single‐Cell Center CAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Energy InstituteQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina College of Information Science&Engineering Ocean University of ChinaQingdaoChina Department of Clinical Laboratory Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Graduate School Peking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Department of Clinical Laboratory Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdaoChina qingdao Single‐Cell Biotechnology Co.Ltd.QingdaoChina The Bioland Laboratory GuangzhouChina

Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and *** the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman mi*** a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman *** Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii *** tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based *** automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.

关键词： antimicrobial susceptibility test laboratory automation Raman sepsis tigecycline

Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第2期8卷 716-729页

作者： Xiang-Peng Tan Yan He jing Yang Xian Wei You-Long Fan Guo-Geng Zhang Yi-Dong zhu Zheng-Qiu Li Hua-Xin Liao Da-jiang qin Xin-Yuan Guan Bin Li The First Affiliated Hospital of jinan University Jinan UniversityGuangzhouChina Key Laboratory of Biological Targeting Diagnosis Therapy and Rehabilitation of Guangdong Higher Education Institutes and Key Laboratory of Protein Modification and DegradationThe Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina MOE Key Laboratory of Tumor Molecular Biology and National Engineering Research Center of Genetic Medicine College of Life Science and TechnologyJinan UniversityGuangzhouChina School of Pharmacy Jinan UniversityGuangzhouChina Department of Clinical Oncology The University of Hong KongHong KongChina

Hepatocellular carcinoma(HCC)is one of the most common malignant *** of the underlying mechanism of HCC progression and exploration of new therapeutic drugs are urgently ***,a compound library consisting of 419 FDA-approved drugs was taken to screen potential anticancer drugs.A series of functional assays showed that desloratadine,an antiallergic drug,can repress proliferation in HCC cell lines,cell-derived xenograft(CDX),patient-derived organoid(PDO)and patient-derived xenograft(PDX)models.N-myristoyl transferase 1(NMT1)was identified as a target protein of desloratadine by drug affinity responsive target stability(DARTS)and surface plasmon resonance(SPR)*** of NMT1 expression enhanced but NMT1 knockdown suppressed tumor growth in vitro and in *** labeling and mass spectrometry analyses revealed that Visinin-like protein 3(VILIP3)was a new substrate of NMT1 in protein N-myristoylation modification,and high NMT1 or VILIP3 expression was associated with advanced stages and poor survival in ***,desloratadine binds to Asn-246 in NMT1 and inhibits its enzymatic activity,disrupting the NMT1-mediated myristoylation of the VILIP3 protein and subsequent NFκB/Bcl-2 ***,this study demonstrates that desloratadine may be a novel anticancer drug and that NMT1-mediated myristoylation contributes to HCC progression and is a potential biomarker and therapeutic target in HCC.

关键词： drugs VILI allergic

ADMETNet：The knowledge base of pharmacokinetics and toxicology network

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2017年第5期44卷 273-276页

作者： Quan Xu Ke Liu Xingming Lin Yangmei qin Linshan Chen jiao Cheng Mindong Zhong Qiushun He Yinbo Li Tingwu Wang jianbo Pan Menglu Peng Lixia Yao Zhiliang ji State Key Laboratory of Stress Cell Biology School of Life SciencesXiamen UniversityXiamen 361102China Department of Health Sciences Research Mayo ClinicRochesterMN 55905USA The Key Laboratory 0r Chemical Biology of Fujian ProvinceXiamen UniversityXiamen 361005China

A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and eliminated out of the body in proper *** processes of drug getting into and out of the body involve Absorption,Distribution,Metabolism,and Excretion（ADME）,

关键词： interact eliminated getting Absorption absorbed manner candidate toxicity Browse Pathway

A novel frameshift mutation in CX46 associated with hereditary dominant cataracts in a Chinese family

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2017年第5期10卷 684-690页

作者： Xiu-Kun Cui Ke-Ke zhu Zheng Zhou Si-Min Wan Yi Dong Xuan-Ce Wang jing Li jing Zhang Hong-Mei Mu Lei qin Yan-Zhong Hu Henan Intemational Union Laboratory of Antibody Medicine Department of Cell biology and Genetics Henan University School of Medicine Kaifeng 475004 Henan Province China Kaifeng Key Lab of Cataract and Myopia Institute of Eye Diseases Kaifeng Centre Hospital Kaifeng 475000 Henan Province China Department of Cardiovascular Medicine Kaifeng Centre Hospital Kaifeng 475000 Henan Province China

AIM：To investigate the genetic mutations that are associated the hereditary autosomal dominant cataract in a Chinese ***：A Chinese family consisting of 20 cataract patients（including 9 male and 11 female） and 2 unaffected individuals from 5 generations were diagnosed to be a typical autosomal dominant cataract pedigree. Genomic DNA samples were extracted from the peripheral blood cells of the participants in this pedigree. Exon sequence was used for genetic mutation screening. In silico analysis was used to study the structure characteristics of connexin 46（CX46） mutant. Immunoblotting was conduceted for testing the expression of ***：To determine the involved genetic mutations, 11 well-known cataract-associated genes（cryaa, cryab, crybb1, crybb2, crygc, crygd, Gja3, Gja8, Hsf4, Mip and Pitx3） were chosen for genetic mutation test by using exon sequencing. A novel cytosine insertion at position 1195 of CX46 c DNA（c.1194_1195ins C） was found in the samples of 5 tested cataract patients but not in the unaffected 2 individuals nor in normal controls, which resulted in 30 amino acids more extension in CX46Cterminus（cx46fs400） compared with the wild-type CX46. In silico protein structure analysis indicated that the mutant showed distinctive hydrophobicity and protein secondary structure compared with the wild-type CX46. The immunoblot results revealed that CX46 protein, which expressed in the aging cataract lens tissues, was absence in the proband lens. In contrast, CX50, alpha A-crystallin and alphaB-crystallin expressed equally in both proband and aging cataract tissues. Those results revealed that the cx46fs400 mutation could impair CX46 protein expression. CONCLUSION：The insertion of cytosine at position 1195 of CX46 cD NA is a novel mutation site that is associated with the autosomal dominant cataracts in this Chinese family. The C-terminal frameshift mutation is involved in regulating CX46 protein expression.

关键词： connexin 46 cataract congenital ocular lens autosome dominant heredity genetic mutation

Temperature‑Arousing Self‑Powered Fire Warning E‑Textile Based on p-n Segment Coaxial Aerogel Fibers for Active Fire Protection in Firefighting Clothing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2023年第12期15卷 141-160页

作者： Hualing He Yi qin Zhenyu zhu qing jiang Shengnan Ouyang Yuhang Wan Xueru Qu jie Xu Zhicai Yu State Key Laboratory of New Textile Materials and Advanced Processing Technologies School of Textile Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China National Local Joint Laboratory for Advanced Textile Processing and Clean Production Hubei Key Laboratory of Biomass Fibers and Eco‑Dyeing and FinishingWuhan Textile UniversityWuhan 430200People’s Republic of China jiangsu New Horizon Advanced Functional Fiber Innovation Center Co. Ltd.Suzhou 215000People’s Republic of China

Firefighting protective clothing is a crucial protective equipment for firefighters to minimize skin burn and ensure safety firefighting operation and rescue mission.A recent increasing concern is to develop self-powered fire warning materials that can be incorporated into the firefighting clothing to achieve active fire protection for firefighters before the protective clothing catches fire on ***,it is still a challenge to facilely design and manufacture thermoelectric(TE)textile(TET)-based fire warning electronics with dynamic surface conformability and ***,we develop an alternate coaxial wet-spinning strategy to continuously produce alternating p/n-type TE aerogel fibers involving n-type Ti_(3)C_(2)T_(x)MXene and p-type MXene/SWCNT-COOH as core materials,and tough aramid nanofiber as protective shell,which simultaneously ensure the flexibility and high-efficiency TE power *** such alternating p/n-type TE fibers,TET-based self-powered fire warning sensors with high mechanical stability and wearability are successfully fabricated through stitching the alternating p-n segment TE fibers into aramid *** results indicate that TET-based fire warning electronics containing 50 p-n pairs produce the open-circuit voltage of 7.5 mV with a power density of 119.79 nW cm-2 at a temperature difference of 300℃.The output voltage signal is then calculated as corresponding surface temperature of firefighting clothing based on a linear relationship between TE voltage and *** fire alarm response time and flame-retardant properties are further *** self-powered fire warning electronics are true textiles that offer breathability and compatibility with body movement,demonstrating their potential application in firefighting clothing.

关键词： Self-powered fire warning Coaxial wet spinning P-n segment thermoelectric fiber Thermoelectric textiles Active fire protection

猪急性腹泻综合征冠状病毒S蛋白多克隆抗体的制备及在检测该病毒感染中的应用

在线全文

学校读者我要写书评

暂无评论

中国预防兽医学报 2024年第5期46卷 499-504页

作者：刘大凯韩郁茹张记宇张燎原冯廷帅杨小曼曾苗苗时洪艳秦毅斌石达冯力中国农业科学院哈尔滨兽医研究所动物疫病防控全国家重点实验室黑龙江哈尔滨150069 广西兽医研究所广西兽医生物技术重点实验室广西南宁530001

为制备猪急性腹泻综合征冠状病毒(SADS-CoV)纤突蛋白(S)的多克隆抗体(PAb),本研究经PCR扩增SADS-Co V S蛋白S1亚基C端结构域(S1-CTD)基因片段(384 bp),并将其克隆至原核表达载体p GEX-6p-1中,构建重组质粒p GEX-6p-1-S1-CTD,经双酶切和... 详细信息

为制备猪急性腹泻综合征冠状病毒(SADS-CoV)纤突蛋白(S)的多克隆抗体(PAb),本研究经PCR扩增SADS-Co V S蛋白S1亚基C端结构域(S1-CTD)基因片段(384 bp),并将其克隆至原核表达载体p GEX-6p-1中,构建重组质粒p GEX-6p-1-S1-CTD,经双酶切和测序鉴定正确后,转化大肠杆菌BL21(DE3)感受态细胞,利用IPTG诱导表达,通过western blot鉴定重组S1-CTD蛋白(rS1-CTD)的表达及反应原性。结果显示,r S1-CTD以包涵体的形式表达,在40 ku处出现特异性条带。诱导表达后的r S1-CTD经不同浓度尿素重悬并超声离心,SDS-PAGE检测后切胶纯化,得到纯化的重组蛋白。利用BCA试剂盒测得蛋白的浓度为33μg/m L。将该重组蛋白乳化后经3次免疫新西兰大白兔,并在3免一周后采血,分离血清获得S1-CTD蛋白PAb。将SADS-Co V感染Vero E6细胞24 h后,以获得的兔PAb为一抗,分别采用western blot和间接免疫荧光试验(IFA)检测该PAb的反应原性。Western blot结果显示,在约250 ku处出现特异性条带,而阴性对照组无该条带;IFA结果显示,SADS-Co V感染的细胞中出现绿色荧光,而阴性对照细胞无绿色荧光。将SADS-Co V感染仔猪的回肠组织制备病理切片,以制备的PAb为一抗,通过免疫组织化学(IHC)检测SADS-Co V的抗原。结果显示,该组织切片中出现棕色阳性信号,而阴性对照仔猪回肠组织切片则无该棕色信号。表明该PAb可与感染SADS-Co V的仔猪回肠组织中的相应抗原发生特异性免疫反应。综上所述,本实验制备的S1-CTD蛋白PAb具有良好的反应原性和免疫原性,可以用于western blot、IFA、IHC检测体内外SADS-Co V的感染,为后续SADS-Co V检测方法的建立及S蛋白生物学功能的研究奠定基础。

关键词：猪急性腹泻综合征冠状病毒 S蛋白原核表达多克隆抗体初步应用

老鼠簕来源内生真菌Diaporthe pseudomagiferae的聚酮类化合物分离鉴定及体外抗氧化、抗炎活性研究

在线全文

学校读者我要写书评

暂无评论

广西医科大学学报 2024年第7期41卷 1035-1041页

作者：潘钰莹韦霞刘妍颖幸尚平潘波覃其云麻晓琳代子腾郭宏伟朱丹广西医科大学药学院南宁530021 广西壮族自治区林业科学研究院南宁530002 广西生物活性分子研究与评价重点实验室南宁530021

目的:研究老鼠簕来源的内生真菌Diaporthe pseudomagiferae中聚酮类化合物分离纯化及其抗氧化、抗炎活性。方法:利用正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等色谱技术进行分离纯化,结合高分辨质谱、核磁共振波谱技术... 详细信息

目的:研究老鼠簕来源的内生真菌Diaporthe pseudomagiferae中聚酮类化合物分离纯化及其抗氧化、抗炎活性。方法:利用正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等色谱技术进行分离纯化,结合高分辨质谱、核磁共振波谱技术等综合谱学技术对化合物进行结构解析,对部分化合物进行抗炎活性评价和抗氧化活性筛选。结果:从Diaporthe pseudomagiferae大米发酵产物中分离鉴定得到7个聚酮类化合物,分别为:[(8-methyl-9-oxabicyclo [4.2.1] nona-2,4-dien-7-yl)-(5-oxo-2H-furan-2-yl)methyl] acetate(1)、2,3-Dihydromycoepoxydiene(2)、Dothiorelone A(3)、Dothiorelone B(4)、Cytosporone B(5)、5,6-dihydroxy-2,3,6-trimethylcyclohex-2-enone(6)、3-(4-Methylphenoxy)propanoic acid(7)。1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除实验结果显示,化合物1~5均未表现出抗氧化活性。酶联免疫吸附试验(ELISA)结果显示,化合物1~5对脂多糖(LPS)诱导的RAW264.7细胞释放的炎症因子白细胞介素-6(IL-6)有一定的抑制作用。结论:化合物1、3、4和6首次从老鼠簕内生真菌Diaporthe pseudomagiferae中分离得到,化合物1~5具有一定的抗炎活性,为进一步探索相关活性物质的抗炎机制奠定基础。

关键词：老鼠簕内生真菌化学成分抗氧化活性抗炎活性

在线全文

学校读者我要写书评

暂无评论

民族药岩黄连生物碱提取与分离工艺研究进展

亚太传统医药 2024年第9期20卷 65-72页

作者：欧夏莲蒋莉李翠刘寒唐春风祝梦琦覃美黄丽容张占江广西壮族自治区药用植物园广西壮族自治区药用植物园国家中医药传承创新中心广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室广西壮族自治区药用植物园广西壮族自治区中药资源智慧创制工程研究中心广西壮族自治区药用植物园西南濒危药材资源开发国家工程研究中心广西壮族自治区药用植物园广西道地药材高品质形成与应用重点实验室

岩黄连是我国民族药材之一，广泛应用于医药行业，有较长的使用历史。生物碱是岩黄连主要活性成分，具有清热解毒、消炎止痛、抗病毒、抗氧化、抗肝硬化及纤维化等多种药理活性作用。随着提取、分离技术的飞快发展，开发利用岩黄连的活... 详细信息

岩黄连是我国民族药材之一，广泛应用于医药行业，有较长的使用历史。生物碱是岩黄连主要活性成分，具有清热解毒、消炎止痛、抗病毒、抗氧化、抗肝硬化及纤维化等多种药理活性作用。随着提取、分离技术的飞快发展，开发利用岩黄连的活性成分已成为近年的研究热点。通过梳理文献信息，综述岩黄连中生物碱的提取、分离与鉴定方法。目前，岩黄连生物碱的提取方法可归纳为乙醇提取法、盐酸-甲醇提取法、甲醇提取法和氯仿提取法，其中乙醇提取法、盐酸-甲醇提取法最为常用；分离方法包括色谱法、有机试剂萃取法、沉淀法、结晶法和树脂吸附法；鉴定方法有薄层色谱法及紫外光谱、红外光谱、质谱和核磁共振光谱等波谱法，但目前一些较为先进的提取、分离方法未见应用于岩黄连生物碱的生产。通过综述岩黄连生物碱提取与分离工艺研究进展，旨在为保护、研究与合理开发利用岩黄连药材提供参考依据。

关键词：岩黄连民族药生物碱提取分离