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Rare Metals 2017年第10期36卷 799-805页

作者： Zhi-Peng Cheng Xiao-Zhong Chu Xiao-Qing Wu ji-ming xu Hui Zhong jing-Zhou Yin jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials Huaiyin Normal University Huaian 223300 China

An easy and effective method of silver nanoplate synthesis technique was created by reducing silver nitrate （AgNO3） with hydroxylamine hydrochloride （NH2OH·HCl） at room temperature. Silver nanoplates of various shapes, including triangular, truncated triangular, hexagonal, and truncated hexagonal, exhibit an average width and thickness of approximately 1 μm and 50 nm, respectively. Silver nanoparticles were acquired by placing polyvinyl pyrrolidone （PVP） in the reaction solution. The produced silver nanoparticles are quasi-spherical in shape and - 100 nm in size. The catalytic activity in the thermal decomposition of ammonium perchlorate （AID） was distinguished by thermogravimetric （TG） analysis and differential scanning calorimetry （DSC）. The outcomes reveal that the addition of silver nanoplates and nanoparticles diminishes the low decomposition temperature of AP by 7 and 14 ℃ and leads to a drop in the high decomposition temperature of AP by 60 and 110 ℃ and a rise in the total DSC heat release by 0.86 and 1.05 kJ.g^-1, respectively.

关键词： Silver nanoparticle Silver nanoplates Formation mechanism Ammonium perchlorate

Abscisic acid-dependent PMT1 expression regulates salt tolerance by alleviating abscisic acid-mediated reactive oxygen species production in Arabidopsis

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Journal of Integrative Plant Biology 2022年第9期64卷 1803-1820页

作者： Qi Yu He jian Feng jin He Qiang Lou Feng Feng Dang ji ming xu Shao jian Zheng jian Li Yang State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou310058 China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou311300 China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou510642 China

Phosphocholine(PCho)is an intermediate metabolite of nonplastid plant membranes that is essential for salt ***,how PCho metabolism modulates response to salt stress remains ***,we characterize the role of phosphoethanolamine N-methyltransferase 1(PMT1)in salt stress tolerance in Arabidopsis thaliana using a T-DNA insertional mutant,geneediting alleles,and complemented *** pmt1 mutants showed a severe inhibition of root elongation when exposed to salt stress,but exogenous ChoCl or lecithin rescued this ***1 also displayed altered glycerolipid metabolism under salt stress,suggesting that glycerolipids contribute to salt ***,pmt1 mutants exhibited altered reactive oxygen species(ROS)accumulation and distribution,reduced cell division activity,and disturbed auxin distribution in the primary root compared with wild-type *** show that PMT1 expression is induced by salt stress and relies on the abscisic acid(ABA)signaling pathway,as this induction was abolished in the aba2-1 and pyl112458 ***,ABA aggravated the salt sensitivity of the pmt1 mutants by perturbing ROS distribution in the root *** together,we propose that PMT1 is an important phosphoethanolamine N-methyltransferase participating in root development of primary root elongation under salt stress conditions by balancing ROS production and distribution through ABA signaling.

关键词： abscisic acid(ABA) oxidative stress plasma membrane phosphoethanolamine N-methyltransferase phospholipid metabolism salt stress

Clinical　significance　of　PCR　in　Helicobacter　pylori　DNA　detection　in　human　gastric　disorders

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World Journal of Gastroenterology 1997年第2期3卷 41-43页

作者： xu Guo ming, ji xu Huai, LI Zhao Shen, MAN Xiao Hua and ZHANG Hong Fu

AIM To investigate the clinical significance of the PCR assay in the diagnosis of gastric H. pylori infection. METHODS HP infection in gastric antral biopsied specimens was identified by the polymerase chain reaction (PCR) to amplify the specific HP urease gene fragments (PCR HP DNA) in 154 patients with gastrointestinal disorders. HP urease genes oligonucleotide primers specific for HP (16s rRNA) were used. Urease test and ELISA for serum anti HP IgG were also used as control. RESULTS PCR HP DNA was detected in 140(91%) of the 154 patients, 114 and 125 were found infected with HP by urease test and ELISA HP IgG, respectively. There was a marked difference in the HP positive rate between the PCR HP DNA and the urease test or ELISA HP IgG ( P <0 05). The rate of HP infection increased with age although a minority of infected people develop signs and symptoms of gastric disorders. HP infection is closely related to adenocarcinoma in gastric antrum and the downbody of the stomach. CLNCLUSION PCR is a sensitive and specific method for the detection of HP in human gastric tissues. Detection of HP DNA in vivo by this approach might improve the clinical diagnosis and epidemiological research of H. pylori infection.

关键词： peptic ulcer gastritis stomach neoplasms Helicobacter pylori Helicobacter infections polymerase chain reaction

Restriction of iron loading into developing seeds by a YABBY transcription factor safeguards successful reproduction in Arabidopsis

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Molecular Plant 2021年第10期14卷 1624-1639页

作者： Li Sun Yun Qi Wei Kang Hao Wu jing Ying Yan jie Na xu Yun Rong Wu Gui Xin Li ji ming xu Nicholas P.Harberd Zhong jie Ding Shao jian Zheng State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Agricultural Experimental Station Zhejiang UniversityHangzhou 310058China College of Agronomy and Biotechnology Zhejiang UniversityHangzhou 310058China Department of Plant Science University of OxfordOxford OX13RBUK Guangdong Laboratory for Lingnan Modern Agriculture College of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642

Iron(Fe)storage in plant seeds is not only necessary for seedling establishment following germination but is also a major source of dietary Fe for humans and other *** of Fe in seeds is known to be low during early seed ***,the underlying mechanism and biological significance remain ***,we show that reduced expression of Arabidopsis YABBY transcription factor INNER NO OUTER(INO)increases embryonic Fe accumulation,while transgenic overexpression of INO results in the opposite *** is highly expressed during early seed development,and decreased INO expression increases the expression of NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1(NRAMP1),which encodes a transporter that contributes to seed Fe *** relatively high embryonic Fe accumulation conferred by decreased INO expression is rescued by the nramp1 loss-of-function *** further demonstrated that INO represses NRAMP1 expression by binding to NRAMP1-specific promoter ***,we found that excessive Fe loading into developing seeds of ino mutants results in greater oxidative damage,leading to increased cell death and seed abortion,a phenotype that can be rescued by the nramp1 *** together,these results indicate that INO plays an important role in safeguarding reproduction by reducing Fe loading into developing seeds by repressing NRAMP1 expression.

关键词： iron seed embryo toxicity transcription factor Arabidopsis

Ethylene promotes seed iron storage during Arabidopsis seed maturation via ERF95 transcription factor

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Journal of Integrative Plant Biology 2020年第8期62卷 1193-1212页

作者： Ying Sun jia Qi Li jing Ying Yan Jun jie Yuan Gui Xin Li Yun Rong Wu ji ming xu Rong Feng Huang Nicholas P.Harberd Zhong jie Ding Shao jian Zheng State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Agricultural Experimental Station Zhejiang UniversityHangzhou 310058China College of Agronomy and Biotechnology Zhejiang UniversityHangzhou 310058China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Department of Plant Science University of OxfordOxford OX13RBUnited Kingdom

Because Iron(Fe)is an essential element,Fe storage in plant seeds is necessary for seedling establishment following ***,the mechanisms controlling seed Fe storage during seed development remain largely *** we reveal that an ERF95 transcription factor regulates Arabidopsis seed Fe *** show that expression of ERF95 increases during seed maturation,and that lack of ERF95 reduces seed Fe accumulation,consequently increasing sensitivity to Fe deficiency during seedling ***,overexpression of ERF95 has the opposite *** show that lack of ERF95 decreases abundance of FER1 messenger RNA in developing seed,which encodes Fe-sequestering ***,a fer1-1 loss-of-function mutation confers reduced seed Fe accumulation,and suppresses ERF95-promoted seed Fe *** addition,ERF95 binds to specific FER1 promoter GCC-boxes and transactivates FER1 *** show that ERF95 expression in maturing seed is dependent on EIN3,the master transcriptional regulator of ethylene *** lack of EIN3 reduces seed Fe content,overexpression of ERF95 rescues Fe accumulation in the seed of ein3 loss-of-function ***,we show that ethylene production increases during seed *** conclude that ethylene promotes seed Fe accumulation during seed maturation via an EIN3-ERF95-FER1-dependent signaling pathway.

关键词： expression. ERF Arabidopsis

论中国当前供需结构性改革思路——基于供需转换与经济持续均衡增长视角

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社会科学 2017年第1期 30-39页

作者：纪明许春慧广西师范学院经济与管理学院广西师范学院科研管理处广西师范学院学报编辑部

供给和需求均对经济持续增长具有重要作用,但供给和需求在经济发展的不同阶段会表现出不同特征。当经济发展由工业化初期阶段进入工业化中期阶段,制约经济持续均衡增长的主要因素由供给总量转换为需求总量;当经济发展进入到工业化中期... 详细信息

供给和需求均对经济持续增长具有重要作用,但供给和需求在经济发展的不同阶段会表现出不同特征。当经济发展由工业化初期阶段进入工业化中期阶段,制约经济持续均衡增长的主要因素由供给总量转换为需求总量;当经济发展进入到工业化中期阶段并向工业化后期及后工业化阶段演进,制约经济持续均衡增长的主导因素将由供需总量逐渐转换为供需结构。相应地,宏观经济政策设计也应根据经济发展的阶段性特征而相机决择。改革开放以来,应对中国经济发展的需求管理与供给管理的宏观经济政策增长效果良好,现阶段和未来一段时间内,中国经济发展已具备工业化后期阶段发展特征,宏观经济政策设计的重点是寻求供给与需求的内部均衡及结构间均衡,以新型城镇化为载体寻求供给侧结构性改革和需求侧结构性改革的思路及突破点,以促进经济持续均衡增长。

关键词：总供给总需求供给结构需求结构新型城镇化经济持续均衡增长

A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis

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Molecular Plant 2021年第9期14卷 1554-1568页

作者： Wen Hao Tian jia Yuan Ye Meng Qi Cui Jun Bo Chang Yu Liu GuiXin Li Yun Rong Wu ji ming xu Nicholas P.Harberd Chuan Zao Mao Chong Wei jin Zhong jie Ding Shao jian Zheng State Key Laboratory of Plant Physiology and Biochemistry College of Life ScienceZhejiang UniversityHangzhou 310058China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310038China College of Agronomy and Biotechnology Zhejiang UniversityHangzhou 310038China Department of Plant Sciences University of OxfordOxford OX13RBUK Guangdong Laboratory for Lingnan Modern Agriculture College of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 5100642China

Phosphorus(P)is an indispensable macronutrient required for plant growth and *** phosphate(Pi)reserves are finite,and a better understanding of Pi utilization by crops is therefore vital for worldwide food *** has long been known to enhance Pi acquisition efficiency in agriculture;however,the molecular mechanisms coordinating Pi nutrition and ammonium remains ***,we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein,STOP1,in the nuclei of Arabidopsis root cells under Pi *** show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root *** acidification-triggered STOP1 accumulation activates the excretion of organic acids,which help to solubilize Pi from insoluble iron or calcium *** uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic *** together,we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate *** findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil.

关键词： Pi deficiency response ammonium STOP1 organic acid exudation CIPK23

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao De-En xu ming-Lei Wu ji-Chuan Liu Zi-Ling Shi Quan-Hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

全雌性黄瓜中3个肌动蛋白基因片段的克隆和表达分析

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细胞生物学杂志 2008年第1期30卷 125-130页

作者：徐纪明向太和杭州师范大学生命与环境科学学院杭州310036

通过1次PCR扩增从全雌性黄瓜中同时获得了长度分别为1186bp、955bp和870bp的3个肌动蛋白基因片段Act1、Act2和Act3(GenBank登记号:DQ115881、DQ115882和DQ115883)。序列分析表明,3个肌动蛋白基因片段与GenBank中收录的肌动蛋白基因序列... 详细信息

通过1次PCR扩增从全雌性黄瓜中同时获得了长度分别为1186bp、955bp和870bp的3个肌动蛋白基因片段Act1、Act2和Act3(GenBank登记号:DQ115881、DQ115882和DQ115883)。序列分析表明,3个肌动蛋白基因片段与GenBank中收录的肌动蛋白基因序列高度同源,与GenBank中收录的全长肌动蛋白基因(如AF386514.1、AF059484.1和AB010922.1等)的第2、第3个外显子和第2个内含子相对应。Act1中1￣580bp、984￣1186bp,Act2中1￣580bp、753￣955bp,Act3中1￣580bp、668￣870bp为外显子编码区,其余为内含子序列,3个基因内含子的两端序列均符合典型的"GT-AG"规则。RT-PCR分析表明,Act1仅在根中表达,Act2在根和雌花中表达,而Act3在所有检测的器官(包括根、茎、叶、卷须、雌花和幼果)中都表达,提示黄瓜根的生长发育需要多种肌动蛋白基因的参与,而Act2和Act3可能与黄瓜雌性系的形成发育有关。

关键词：黄瓜肌动蛋白基因克隆表达

温下方乙酸乙酯提取部位调控CAFs介导的Hh-Gli1通路抑制A549移植瘤生长的作用机制

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中华中医药杂志 2021年第9期36卷 5499-5504页

作者：王盟毕倩宇季旭明浙江中医药大学基础医学院杭州310053 济宁市第一人民医院医务部济宁272000 浙江中医药大学基础医学院方剂教研室杭州310053

目的:观察温下方乙酸乙酯提取部位(WFEA)对肿瘤相关成纤维细胞(CAFs)介导的Hh-Gli1通路的影响,探讨其抑制A549移植瘤生长的作用机制。方法:建立肺癌A549裸鼠皮下移植瘤模型,随机分为A549组、模型组、顺铂组,WFEA高、中、低剂量组和环巴... 详细信息

目的:观察温下方乙酸乙酯提取部位(WFEA)对肿瘤相关成纤维细胞(CAFs)介导的Hh-Gli1通路的影响,探讨其抑制A549移植瘤生长的作用机制。方法:建立肺癌A549裸鼠皮下移植瘤模型,随机分为A549组、模型组、顺铂组,WFEA高、中、低剂量组和环巴胺组,药物干预5周。采用超高压液相色谱-飞行时间质谱仪(UPLC-Q-TOF-MS)分析WFEA的主要化学成分;采用小动物活体成像、HE染色、免疫组化等方法检测WFEA在A549移植瘤裸鼠体内的抗肿瘤活性;采用Western blot法和实时PCR法检测CAFs介导的Hh-Gli1信号通路水平。结果:采用UPLC-Q-TOF-MS方法共定性出WFEA的15种化学成分;与模型组比较,WFEA各给药组裸鼠肿瘤的光子强度均有不同程度的降低(P<0.05,P<0.01)。模型组HE染色可见细胞排列紧密,瘤细胞形态不规则,未见明显的坏死,WFEA各给药组肿瘤细胞均有不同程度坏死,胞浆破裂,细胞膜不完整;与模型组比较,WFEA各给药组FN蛋白表达以及PTCH1、SMO和Gli1的蛋白和mRNA表达水平均有不同程度的降低(P<0.01,P<0.05)。结论:WFEA对A549移植瘤有一定的抑制作用,其作用机制可能与抑制CAFs介导的Hh-Gli1信号通路有关。

关键词：温下方肿瘤相关成纤维细胞(CAFs) 超高压液相色谱-飞行时间质谱仪(UPLC-Q-TOF-MS) Hh-Gli1 乙酸乙酯提取部位 A549移植瘤肺癌