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Pulmonary embolism and deep vein thrombosis caused by nitrous oxide abuse: A case report

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World Journal of Clinical Cases 2019年第23期7卷 4057-4062页

作者： Wen Sun ji-Ping liao Yan Hu Wei Zhang jing Ma Guang-Fa Wang Department of Pulmonary and Critical Care Medicine Peking University First Hospital

BACKGROUND Nitrous oxide(N2O)has gained increasing popularity as a recreational drug,causing hallucinations,excitation,and psychological ***,side effects have been reported in recent *** case report proposes a correlation among N2O,pulmonary embolism(PE),and deep vein thrombosis(DVT)and emphasizes the role of homocysteine(Hcy)in thrombotic *** SUMMARY A 29-year-old man with long-term N2O abuse sought evaluation after acute chest pain.A diagnostic workup revealed PE,DVT,and *** patient was successfully treated with thrombolytic and anticoagulant ***,his Hcy level decreased and returned to normal after Hcy-lowering *** Chronic N2O abuse might increase the risk of PE and DVT,although there have been few studies previously.

关键词： Nitrous oxide Pulmonary embolism Deep vein thrombosis Homocysteine Case report

MYPT1 Sustains Centromeric Cohesion and the Spindle-Assembly Checkpoint

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Journal of Genetics and Genomics 2013年第11期40卷 575-578页

作者： jing Li Xiaoqian Liu ji liao jie Tian Jue Wang Xin Wang jiezhong Zhang Xingzhi Xu Beijing Key Laboratory of DNA Damage Response College of Life SciencesCapital Normal University

During mitosis, cohesins hold the sister chromatids together until anaphase when arm cohesins are removed （Peters et al., 2008; Yao and Dai, 2012）. The shugoshin （Sgo） proteins play pivotal roles during this stage. There is only one shu- goshin in the fly and budding yeasts, while there are two in other organisms （including fission yeasts）. The two mamma- lian shugoshins, Sgol and Sgo2, carry out distinct functions： Sgol mainly in mitosis, and Sgo2 mainly in meiosis and perturbed mitosis. Mitotic cyclin-dependent kinase 1 （CDKI） phosphorylates Sgol, and targets the Sgol-protein phospha- tase 2A （PP2A） complex to protect centromeric cohesin （Kitajima et al., 2006; Tang et al., 2006; Liu et al., 2012）,

关键词： MYPT1 Sustains Centromeric Cohesion and the Spindle-Assembly Checkpoint RNAi PP

Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer

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World Journal of Gastroenterology 2015年第34期21卷 9916-9926页

作者： Long-Jun He Dan Xie Pin-jin Hu Yi-ji liao Hai-Xia Deng Hsiang-Fu Kung Sen-Lin Zhu Department of Gastroenterology the First Affiliated HospitalSun Yat-sen University Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Stanley Ho Center for Emerging Infectious Diseases and Li Ka Shing Institute of Health Sciences State Key Laboratory of Oncology in South China The Chinese university of Hong Kong

AIM:To investigate macrophage migration inhibitory factor(MIF) expression and its clinical relevance in gastric cancer,and effects of MIF knockdown on proliferation of gastric cancer cells. METHODS:Tissue microarray containing 117 samples of gastric cancer and adjacent non-cancer normal tissues was studied for MIF expression by immunohistochemistry(IHC) semiquantitatively,and the association of MIF expression with clinical parameters was analyzed. MIF expression in gastric cancer cell lines was detected by reverse transcriptionpolymerase chain reaction(RT-PCR) and Western blot. Two pairs of si RNA targeting the MIF gene(MIF si-1 and MIF si-2) and one pair of scrambled si RNA as a negative control(NC) were designed and chemically synthesized. All si RNAs were transiently transfected in AGS cells with OligofectamineTM to knock down the MIF expression,with the NC group and mock group(OligofectamineTM alone) as controls. At 24,48,and 72 h after transfection,MIF m RNA was analyzed by RTPCR,and MIF and proliferating cell nuclear antigen(PCNA) proteins were detected by Western *** proliferative rate of AGS cells was assessed by methylthiazolyl tetrazolium(MTT) assay and colony forming ***:The tissue microarray was informative for IHC staining,in which the MIF expression in gastric cancer tissues was higher than that in adjacent noncancer normal tissues(P < 0.001),and high level of MIF was related to poor tumor differentiation,advanced T stage,advanced tumor stage,lymph node metastasis,and poor patient survival(P < 0.05 for all). After si RNA transfection,MIF m RNA was measured by real-time PCR,and MIF protein and PCNA were assessed by Western blot analysis. We found that compared to the NC group and mock group,MIF expression was knocked down successfully in gastric cancer cells,and PCNA expression was downregulated with MIF knockdown as well. The cell counts and the doubling times were assayed by MTT 4 d after transfection,and colonies formed were assayed by

关键词： Macrophage migration inhibitory factor Proliferati

Vegetation Restoration Technique System and Its Ecological Functions of the Degraded Ecosystem in the Arid-Hot Valleys——Taking the Example of Typical Mode in Small Watershed of Yuanmou

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Wuhan University Journal of Natural Sciences 2008年第3期13卷 257-266页

作者： ji Zhonghua liao Chengfei YANG Yanxian FANG Haidong PAN Zhixian SHA Yucang Institute of Tropical Eco-Agricultural Sciences Yunnan Academy ofAgricultural Sciences Yuanmou 651300 Yunnan China

This paper concluded the vegetation restoration technique system in the arid-hot valleys and studied the anti-erosion function, environmental function and biological diversity effects of vegetation restoration on the ecosystem in the arid-hot valleys. The results showed that the soil erodibility decreased significantly after the vegetation restoration. The climate environment of the small watershed had a great improvement after the vegetation restoration, of which the temperature decreased, the humidity increased, the harsh environment of dry and hot in this region changed. The studies of the ecosystem biodiversity were mainly on the analysis of the relations between biodiversity and ecological function of the artificial ecological forest pattern and the natural enclosed treatment mode on the severely of degraded land. It could conclude that the natural enclosed treatment mode is helpful to the biodiversity of the ecosystem and the improvement and stability of the ecosystem, and Leucaena artificial forest restoration pattern reduced the species diversity but optimized the ecological function. Therefore, as to the severely and extremely severely degraded ecosystem in the arid-hot valleys, Leucaena pattern of gully control and natural enclosed treatment mode are the relatively optimal choices.

关键词： vegetation restoration technique system anti-erosion function environmental function biodiversity

Molecular self-induced configuration for improving dissymmetry factors in tetradentate platinum(Ⅱ) enantiomers cycloaddition

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Chinese Chemical Letters 2022年第3期33卷 1459-1462页

作者： Li Yuan Qian-Jun Ding Zhen-Long Tu Xiang-ji liao Xu-Feng Luo Zhi-Ping Yan Zheng-Guang Wu You-Xuan Zheng State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Nantong Key Lab of Intelligent and New Energy Materials College of Chemistry and Chemical EngineeringNantong UniversityNantong 226019China

Two pairs of Pt(Ⅱ) enantiomers((RR)/(SS)-Py Pt,((RR)/(SS)-Py: N,N’-(1,2-diphenylethane-1,2-diyl)dipicolinamide;(RR)-P/M-QPt,((RR)/(SS)-Q: N,N’-((1R,2R)-1,2-diphenylethane-1,2-diyl)bis(quinoline-2-carboxamide)) were synthesized, respectively, with good circularly polarized luminescence(CPL) and tunable dissymmetry factors(g) by molecular self-induction with(RR)/(SS)-1,2-diphenylethane-1,2-diamine as carbon chiral sources. In the(RR)-P-QPt and(SS)-M-QPt, specific P-and M-configurations were effectively induced from intrinsic chiral carbon centres(R or S), ingeniously avoiding the racemic mixture formation and chiral separation. Furthermore, the chirality originating from both chiral carbon centres and helicene-like structure improves the g factor significantly, which provides a new molecular design strategy for chiral Pt(II) enantiomers with good CPL properties.

关键词： Pt(Ⅱ)enantiomer Circularly polarized luminescence Dissymmetry factor Molecular self-induction Helicene-like structure

Cytokinesis and cancer:Polo loves ROCK'n' Rho(A)

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Journal of Genetics and Genomics 2010年第3期37卷 159-172页

作者： jing Li Jue Wang Hong jiao ji liao Xingzhi Xu Laboratory of Cancer Biology College of Life Science Capital Normal University Beijing 100048 China

Cytokinesis is the last step of the M （mitosis） phase, yet it is crucial for the faithful division of one cell into two. Cytokinesis failure is often associated with cancer. Cytoldnesis can be morphologically divided into four steps： cleavage furrow initiation, cleavage furrow ingression, midbody formation and abscission. Molecular studies have revealed that RhoA as well as its regulators and effectors are im- portant players to ensure a successful cytokinesis. At the same time, Polo-like kinase 1 （Plkl） is an important kinase that can target many substrates and carry out different functions during mitosis, including cytokinesis. Recent studies are beginning to unveil a closer tie be- tween Plkl and RhoA networks. More specifically, Plkl phosphorylates the centralspindlin complex Cyk4 and MKLPI/CHO1, thus re- cruiting RhoA guanine nucleotide-exchange factor （GEF） Ect2 through its phosphopeptide-binding BRCT domains. Ect2 itself can be phosphorylated by Plkl in vitro. Plkl can also phosphorylate another GEF MyoGEF to regulate RhoA activity. Once activated, RhoA-GTP will activate downstream effectors, including ROCK1 and ROCK2. ROCK2 is among the proteins that associate with Plkl Polo-binding domain （PBD） in a large proteomic screen, and Plkl can phosphorylate ROCK2 in vitro. We review current understandings of the interplay between Plkl, RhoA proteins and other proteins （e.g., NudC, MKLP2, PRC 1, CEP55） involved in cytokinesis, with particular emphasis of its clinical implications in cancer.

关键词： Polo-like kinase 1 RhoA GTPase Rho kinase cytokinesis

Influence of phase components of tungsten oxide on homogeneity of ultrafine tungsten powder

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中国有色金属学会会刊：英文版 2000年第3期10卷 421-423页

作者： liao ji qiao(廖寄乔) ZOU Zhi qiang(邹志强) L Hai bo(吕海波) CHEN Shao yi(陈绍衣) HUANG Bai yun(黄伯云) Cent S Univ Technol Natl Key Lab Powder Met Changsha 410083 Peoples R China

The influence of phase components of tungsten oxide on homogeneity of ultrafine tungsten powder by conventional hydrogen reduction techniques was studied. Results show that phase components of tungsten oxide play a crucial role on homogeneity of metal tungsten powder; ultrafine and homogeneous tungsten powder can be produced from oxides which consist of only one phase. Due to the different reduction rates (or different reduction paths) of oxide which comprises different phases, the multi phase components tungsten oxide leads to a fine but in homogeneous metal tungsten powder.

关键词： tungsten oxide phase components ultrafine tungsten powder homogeneity

Physicochemical Characteristics of Orange Juice Samples From Seven Cultivars

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Agricultural Sciences in China 2008年第1期7卷 41-47页

作者： NIU Li-ying WU ji-hong liao Xiao-jun CHEN Fang WANG Zheng-fu ZHAO Guang-hua HU Xiao-song College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 P.R.China

Some physicochemical parameters of orange juice from seven orange varieties （Pineapple orange, Hamlin, Trovita, jincheng, 1232 Tangor, Olinda Valencia, and Delta Valencia） were analyzed, special attention was paid to the level of sugars, organic acids, free amino acids, mineral composition, and color parameters. The results showed that total soluble solids （TSS）, TSS/titratable acidity （TA） ratios, sugars, and organic acids were kept within a proper range for juice processing except that jincheng had lower TSS than the others. Sucrose was the most dominant sugar in the orange cultivars, followed by fructose and glucose, while citric acid was the principal organic acid. Serine and proline were the principal amino acids. Potassium was the most abundant mineral in all of the juice samples, followed by magnesium and calcium. The sodium content of most varieties was under 10 mg kg^-1. Though all of the samples had orange colour, Olinda Valencia and Delta Valencia had deeper red colour than the others. Statistically significant differences （P〈 0.05） were found between the samples in most of the characteristics. The results provided important information on the physicochemical characteristics of the varieties and on how to make the best use of orange cultivars for different purposes, which is of significance for both technological research and processing practice.

关键词： physicochemical characteristics orange cultivars China

Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

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Journal of Integrative Agriculture 2012年第1期11卷 116-121页

作者： GUO Li-li ZHANG jian-min XU Cheng-gang REN Tao ZHANG Bin CHEN ji-dang liao Ming Key Laboratory of Animal Disease Control and Prevention Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of GuangdongCollege of Veterinary MedicineSouth China Agricultural UniversityGuangzhou 510642P.R.China

To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis （PFGE）. Overall, the commonly detected resistance phenotypes were resistant to ampicillin （26.09%）, penicillin （22.61%）, amoxicillin （21.74%）, cefazolin （14.78%）, cefaclor （12.17%）, and cefotaxime （6.96%）. These strains showed high minimal inhibitory concentration （MICs） to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase （ESBL） phenotype. Moreover, 19 strains harboured bla genes including TEM-1 （n=5）, TEM-116 （n=10）, and ROB-1 （n=5）. Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime （MIC=8 mg L-1）. The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.

关键词： Haemophilus parasuis β-lactamase genes resistance pulsed-field gel electrophoresis