Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

作     者：GUO Li-li ZHANG Jian-min XU Cheng-gang REN Tao ZHANG Bin CHEN Ji-dang LIAO Ming GUO Li-li*, ZHANG Jian-min*, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang and LIAO Ming Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China

作者机构：Key Laboratory of Animal Disease Control and PreventionMinistry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of GuangdongCollege of Veterinary MedicineSouth China Agricultural UniversityGuangzhou 510642P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第1期

页      面：116-121页

核心收录：

学科分类：090603[农学-临床兽医学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 09[农学] 0906[农学-兽医学] 10[医学] 

基　　金：supported by the Program for New Century Excellent Talents in University,China (NCET-06-0752) the Guangdong Technology Planning Committee,China (2006B0152 and 2009A0201006) 

主　　题：Haemophilus parasuis β-lactamase genes resistance pulsed-field gel electrophoresis 

摘      要：To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis （PFGE）. Overall, the commonly detected resistance phenotypes were resistant to ampicillin （26.09%）, penicillin （22.61%）, amoxicillin （21.74%）, cefazolin （14.78%）, cefaclor （12.17%）, and cefotaxime （6.96%）. These strains showed high minimal inhibitory concentration （MICs） to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase （ESBL） phenotype. Moreover, 19 strains harboured bla genes including TEM-1 （n=5）, TEM-116 （n=10）, and ROB-1 （n=5）. Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime （MIC=8 mg L-1）. The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.