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Exploring Sequence Space to Design Controllable G-Quadruplex Topology Switches

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CCS Chemistry 2022年第9期4卷 3036-3050页

作者： Jielin Chen Mingpan Cheng Petr Stadlbauer JiříŠponer jean-louis Mergny Huangxian Ju Jun Zhou State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023 Institute of Biophysics of the Czech Academy of Sciences Královopolská135Brno 61265 Regional Centre of Advanced Technologies and Materials Czech Advanced Technology and Research Institute(CATRIN)Palacky University OlomoucŠlechtitelů241/27Olomouc–Holice 78371 Laboratoire d’Optique et Biosciences Ecole PolytechniqueCNRSINSERMInstitut Polytechnique de ParisPalaiseau 91128

As nonclassical nucleic acid structures,G-quadruplexes(G4s)not only play important roles in gene regulation and stability maintenance,but are also widely used in *** diversity is one of the main factors explaining the popularity of G4s,but a comprehensive and integrated study of different factors determining G4 structural versatility is currently lacking.

关键词： G-quadruplex G-quadruplex topology Gswitch sequence composition structural versatility

Epidural anesthesia improves pancreatic perfusion and decreases the severity of acute pancreatitis

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World Journal of Gastroenterology 2015年第43期21卷 12448-12456页

作者： Samira M Sadowski Axel Andres Philippe Morel Eduardo Schiffer jean-louis Frossard Alexandra Platon Pierre-Alexandre Poletti Leo Bühler Department of Visceral and Transplantation Surgery University Hospitals of Geneva Department of Anesthesiology University Hospital Geneva Department of Gastroenterology University Hospital Geneva Department of Radiology University Hospital Geneva

AIM: To study the safety of epidural anesthesia(EA),its effect on pancreatic perfusion and the outcome of patients with acute pancreatitis(AP).METHODS: From 2005 to August 2010,patients with predicted severe AP [Ranson score ≥ 2,C-reactive protein > 100 or necrosis on computed tomography(CT)] were prospectively randomized to either a group receiving EA or a control group treated by patientcontrolled intravenous analgesia. Pain management was evaluated in the two groups every eight hours using the visual analog pain scale(VAS). Parameters for clinical severity such as length of hospital stay,use of antibiotics,admission to the intensive care unit,radiological/clinical complications and the need for surgical necrosectomy including biochemical data were recorded. A CT scan using a perfusion protocol was performed on admission and at 72 h to evaluate pancreatic blood flow. A significant variation in blood flow was defined as a 20% difference in pancreatic perfusion between admission and 72 h and was measured in the head,body and tail of the ***: We enrolled 35 patients. Thirteen were randomized to the EA group and 22 to the control group. There were no differences in demographic characteristics between the two groups. The Balthazar radiological severity score on admission was higher in the EA group than in the control group(mean score 4.15 ± 2.54 vs 3.38 ± 1.75,respectively,P = 0.347) and the median Ranson scores were 3.4 and 2.7 respectively(P = NS). The median duration of EA was 5.7 d,and no complications of the epidural procedure were reported. An improvement in perfusion of the pancreas was observed in 13/30(43%) of measurements in the EA group vs 2/27(7%) in the control group(P = 0.0025). Necrosectomy was performed in 1/13 patients in the EA group vs 4/22 patients in the control group(P = 0.63). The VAS improved during the first ten days in the EA group compared to the control group(0.2 vs 2.33,P = 0.034 at 10 d). Length of stay and mortality were not

关键词： Severe acute pancreatitis Epidural anes thesia Pan

Evaluation of Doppler-ultrasonography in the diagnosis of transjugular intrahepatic portosystemic shunt dysfunction: A prospective study

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World Journal of Hepatology 2017年第27期9卷 1125-1132页

作者： Charlotte Nicolas Amelie Le Gouge louis d’Alteroche jean Ayoub Monica Georgescu Vincent Vidal Denis Castaing jean-Pierre Cercueil Patrick Chevallier Jerome Roumy Herve Trillaud louis Boyer Vincent Le Pennec Christophe Perret Bruno Giraudeau jean-Marc Perarnau STIC-TIPS group Service d’Hepato-Gastroenterologie Hopital TrousseauCHRU Tours CIC CHRU de Tours INSERM United’echographie-doppler Hopital TrousseauCHRU Tours Service de Radiologie Hopital de la Timone Centre Hepato-Biliaire Hopital Paul Brousse Service de Radiologie CHRU Dijon Service d’Imagerie Medicale Diagnostique et interventionnelle Hopital de l’ArchetⅡNice Service de Radiologie et Echographie CHRU Poitiers Service d’Imagerie Medicale Hopital Saint Andre CHRU Bordeaux Service d’Imagerie viscerale et vasculaire CHRU Clermont Ferrand Service de Radiologie CHRU Caen Service de Radiologie CHRU Nantes 不详

AIM To prospectively evaluate the performance of Dopplerultrasonography(US) for the detection of transjugular intrahepatic portosystemic shunt(TIPS) dysfunction within a multicenter cohort of cirrhotic *** This study was conducted in 10 french teaching hospitals. After TIPS insertion, angiography and liver Doppler-US were carried out every six months to detect dysfunction(defined by a portosystemic gradient ≥ 12 mm Hg and/or a stent stenosis ≥ 50%). The association between ultrasonographic signs and dysfunction was studied by logistic random-effects models, and the diagnostic performance of each Doppler criterion was estimated by the bootstrap method. This study was approved by the ethics committee of *** Two hundred and eighteen pairs of examinations performed on 87 cirrhotic patients were analyzed. Variables significantly associated with dysfunction were: The speed of flow in the portal vein(P = 0.008), the reversal of flow in the right(P = 0.038) and left(P = 0.049) portal branch, the loss of modulation of portal flow by the right atrium(P = 0.0005), ascites(P = 0.001) and the overall impression of the operator(P = 0.0001). The diagnostic performances of these variables were low; sensitivity was < 58% and negative predictive value was < 73%. Therefore, dysfunction cannot be ruled out from Doppler-US. CONCLUSION The performance of Doppler-US for the detection of TIPS dysfunction is poor compared to angiography. New tools are needed to improve diagnosis of TIPS dysfunction.

关键词： Transjugular intrahepatic portosystemic shunt Dysfunction Doppler-ultrasonography

一株抗氧化活性酵母菌产多糖的纯化与结构分析

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分析化学 2010年第3期38卷 409-412页

作者：陈历水马莺刘天一 jean-louis Maubois 哈尔滨工业大学食品科学与工程学院 Laboratoire de Recherches de Technologie Laitière INRA65 rue de Saint Brieuc

研究了Pichia fermentans酵母菌产多糖YP5的单糖组成和结构。将酵母菌进行超声破碎后,采用乙醇沉淀法得到粗多糖,然后进行DEAE-纤维素色谱柱分离,得到均一的水溶性多糖YP5。经气相色谱分析表明,糖基由鼠李糖、阿拉伯糖、木糖、甘露糖、... 详细信息

研究了Pichia fermentans酵母菌产多糖YP5的单糖组成和结构。将酵母菌进行超声破碎后,采用乙醇沉淀法得到粗多糖,然后进行DEAE-纤维素色谱柱分离,得到均一的水溶性多糖YP5。经气相色谱分析表明,糖基由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖与半乳糖组成,其摩尔比为9.51∶1.33∶2.31∶0.94∶84.93∶0.96。乌氏粘度计法测得多糖的分子量为8.3×104。结合红外光谱和核磁共振1HNMR分析表明,该多糖YP5具有多糖特征性的红外吸收峰,存在酰胺结构,主要由β-D-葡聚糖组成。

关键词：酵母多糖纯化单糖组成结构

Macrophage populations and self-renewal:Changing the paradigm

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World Journal of Immunology 2015年第3期5卷 131-141页

作者： Rym Belhareth jean-louis Mège URMITE Faculté de Médecine Laboratoire de Neurophysiologie Fonctionnelle et Pathologies UR/11ES09 FST Campus Universitaire

The origin of macrophages has been considered since several decades to be a continuum from bone marrow （BM） to tissue via monocytes as precursors. The development of new tools such as genetic lineage tracing,parabiosis and BM chimeras changed the paradigm of macrophage origin. In steady state, most resident macro-phages are of embryonic origin, whereas a monocyte origin remains prominent in pathological conditions. The findings of a proliferation of mature macrophages will oblige us to reappraise the relationship between proliferation and differentiation in macrophages. This review is based on the recent explosion of high impact articles on macrophage biology. It summarizes new data on the origin of macrophages and their self-renewal potential in steady states. While monocytes are required for intestinal macrophage development, the microglia is independent of monocyte influx and skin macrophages provide an excellent model of the balance between monocyte input and self-renewal. In addition, macrophage proliferation requires intrinsic and extrinsic factors including growth factors and cytokines. It also analyzes the impact of this new paradigm in human diseases such as athrosclerosis, cancer, infe-ctious diseases and neurodegenerative diseases. In atherosclerosis, the fnding of macrophage proliferation within the lesions will change our understanding of disease pathophysiology, this new paradigm may have therapeutical impact in the future.

关键词： Macrophages Self-renewal Proliferation Homeostasis Diseases

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迎接市场复苏推进数字化加工

工具技术 2017年第1期51卷 I0002-I0003页

作者： jean—louis MACEY 山特维克可乐满大中华区

2016年山特维克可乐满的业务发展和中国机械加工市场一样都经历了一个先抑后扬的过程。在上半年，市场形势依旧低迷，我们也面临着很大的挑战，承受着很大的压力；进入下半年，中国制造业逐渐释放出积极的信号，机械行业开始回暖，尤其... 详细信息

2016年山特维克可乐满的业务发展和中国机械加工市场一样都经历了一个先抑后扬的过程。在上半年，市场形势依旧低迷，我们也面临着很大的挑战，承受着很大的压力；进入下半年，中国制造业逐渐释放出积极的信号，机械行业开始回暖，尤其到了第四季度，复苏的动力更加明显。一系列宏观经济数据给了我们很大的信心。制造业采购经理人指数、

关键词：市场形势数字化加工中国制造业机械加工业务发展机械行业经济数据经理人

Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples

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World Journal of Gastroenterology 2006年第21期12卷 3344-3351页

作者： Henrik Laurell Michèle Bouisson Philippe Berthelémy Philippe Rochaix Sébastien Déjean Philippe Besse Christiana Susini Lucien Pradayrol Nicole Vaysse louis Buscail INSERM U531 IFR31 Institut Louis BugnardBP 8422531432 Toulouse Cedex 4France Service de Gastroentérologie Centre Hospitaller de Pau PauFrance Laboratoire d'Anatomo-Pathologie Institut Claudius Regaud ToulouseFrance Laboratoire de Statistique et Probabilités Université Paul Sabatier ToulouseFrance INSERM U531 IFR31 Institut Louis BugnardBP 8422531432 Toulouse Cedex 4France Service de Gastroenterologie et de la NutritionCHU RangueilToulouseFrance

AIM： To compare gene expression profiles of pancreatic adenocarcinoma tissue specimens, human pancreatic and colon adenocarcinoma and leukemia cell lines and normal pancreas samples in order to distinguish differentially expressed genes and to validate the differential expression of a subset of genes by quantitative real-time RT-PCR （RT-QPCR） in endoscopic ultrasound-guided fine needle aspiration （EUS-guided FNA） ***： Commercially dedicated cancer cDNA macroarrays （Atlas Human Cancer 1.2） containing 1176 genes were used. Different statistical approaches （hierarchical clustering, principal component analysis （PCA） and SAM） were used to analyze the expression data. RT-QPCR and immunohistochemical studies were used for validation of ***： RT-QPCR validated the increased expression of LCN2 （lipocalin 2） and for the first time PLAT （tissue-type plasminogen activator or tPA） in malignant pancreas as compared with normal pancreas. Immunohistochemical analysis confirmed the increased expression of LCN2 protein localized in epithelial cells of ducts invaded by carcinoma. The analysis of PLAT and LCN2 transcripts in 12 samples obtained through EUS-guided FNA from patients with pancreatic adenocarcinoma showed significantly increased expression levels in comparison with those found in normal tissues, indicating that a sufficient amount of high quality RNA can be obtained with this ***： Expression profiling is a useful method to identify biomarkers and potential target genes. Molecular analysis of EUS-guided FNA samples in pancreatic cancer appears as a valuable strategy for the diagnosis of pancreatic adenocarcinomas.

关键词： Pancreas Colon Adenocarcinoma Gene expression profiling Endoscopic ultrasonography Ultrasound Fine needle aspiration

Channel Transmission Behavioral Modeling Dedicated to WSNs

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Journal of Energy and Power Engineering 2012年第7期6卷 1042-1049页

作者： Nasreddine Nadim Boizard jean louis Christophe Escriba Fourniols jean Yves Laboratory for Analysis and Architecture of Systems LAAS University of Toulouse Toulouse F-31077 France

The design, manufacture and deployment of embedded systems become increasingly complex and multidisciplinary process. Before the steps of manufacturing and deployment, a simulation and validation phase is necessary. Given the increasing complexity of systems such as telecommunications systems, control systems and others, a specific simulation and validation process must take place. This simulation ideally made in a single development environment should cover different areas and all components of the system. In this paper, the authors briefly describe the behavioral models of the elements of a large scale WSN （wireless sensors network） used to create simulator, focusing specifically on the model of the transmission channel, and how it can retrieve results from the behavioral simulator. In side to legacy network simulator, for the testing and modeling of communication protocols, this simulator should not omit WSN specific aspect, in accuracy it covers the modeling of node platforms, protocols, and real world phenomena.

关键词： WSN VHDL-AMS BER PER.

Embedded FPGA Simulator for Wireless Sensor Network Design

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Journal of Energy and Power Engineering 2012年第6期6卷 984-992页

作者： Nasreddine Nadim Boizard jean louis Christophe Escriba Foumiols jean Yves Laboratory for Analysis and Architecture of Systems University of Toulouse Toulouse F-31077 France

The design, manufacture and deployment of embedded systems become increasingly complex and multidisciplinary process. Before the steps of manufacturing and deployment, a simulation and validation phase is necessary. In this paper, a WSN （wireless sensors network） emulator, implemented on a FPGA, is described by： describing some models as examples, how multiple simulations can be done when the device was configured and programmed once, and how results from the behavioral emulator can be extracted. The diversity of applications and the typically scarce node resources set very tight constraints to WSN. It is not possible to fulfill all requirements with a general purpose WSN, for which reason the rapid development of application specific WSNs is preferred. A new emulator for the design, simulation, and evaluation of WSNs, implemented on a very dense FPGA （field programmable gate arrays）, is presented, exploring the parallelism and his resources to do a very rapid simulation ofa WSN for long periods, and without overloading memory by the intermediate data.

关键词： WSN FPGA VHDL BER PER.