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Rapid species identification of pathogenic bacteria from a minute quantity exploiting three-dimensional quantitative phase imaging and artificial neural network

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Light(Science & Applications) 2022年第7期11卷 1595-1606页

作者： Geon Kim Daewoong Ahn Minhee Kang Jinho Park DongHun ryu YoungJu Jo Jinyeop Song jea sung ryu Gunho Choi Hyun Jung Chung Kyuseok Kim Doo Ryeon Chung In Young Yoo Hee Jae Huh Hyun-seok Min Nam Yong Lee YongKeun Park Department of Physics Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea KAIST Institute for Health Science and Technology KAISTDaejeon 34141Republic of Korea Tomocube Inc. Daejeon 34109Republic of Korea Smart Healthcare&Device Research Center Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea Graduate School of Nanoscience and Technology Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Department of Biological Sciences Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Department of Emergency Medicine Bundang CHA HospitalSeongnam-siGyeonggi-Do 13496Korea Division of Infectious Diseases Department of Internal MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea Department of Laboratory Medicine Seoul St.Mary's HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591Republic of Korea Department of Laboratory Medicine and Genetics Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Republic of Korea Department of Applied Physics Stanford UniversityStanfordCA 94305USA Department of Physics Massachusetts Institute of TechnologyCambridgeMA 02139USA

The healthcare industry is in dire need of rapid microbial identification techniques for treating microbial *** infections are a major healthcare issue worldwide,as these widespread diseases often develop into deadly *** studies have shown that an early appropriate antibiotic treatment significantly reduces the mortality of an infection,this effective treatment is difficult to *** main obstacle to early appropriate antibiotic treatments is the long turnaround time of the routine microbial identification,which includes time-consuming sample ***,we propose a microscopy-based framework that identifies the pathogen from single to few *** framework obtains and exploits the morphology of the limited sample by incorporating three-dimensional quantitative phase imaging and an artificial neural *** demonstrate the identification of 19 bacterial species that cause bloodstream infections,achieving an accuracy of 82.5%from an individual bacterial cell or *** performance,comparable to that of the gold standard mass spectroscopy under a sufficient amount of sample,underpins the effectiveness of our framework in clinical ***,our accuracy increases with multiple measurements,reaching 99.9%with seven different measurements of cells or *** believe that our framework can serve as a beneficial advisory tool for clinicians during the initial treatment of infections.

关键词： artificial neural exploit

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Numerical study on the heat dissipation characteristics of high-power LED module

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Science China(Technological Sciences) 2013年第9期56卷 2150-2155页

作者： LEE DaeWoo CHO sung-Wook KIM Youn-jea Graduate School of Mechanical Engineering Sungkyunkwan University School of Mechanical Engineering Sungkyunkwan University

LED lighting has problems such as short life span, power reduction due to the LED chip's heat, and decreased luminance efficiency etc. Many studies on the optimization of the thermal performance of LEDs have been conducted actively to solve these problems. The present study changes the fin shape and intervals of various heat sink designs so that LED can effectively produce a thermal effect. The water-cooling heat release system and the heat pipe methods show higher thermal performance results than the existing systems; however, the system is of high cost and requires a large-scale apparatus. This study carried out a numerical analysis of the thermal control system by applying heat flux and heat sink properties as an active heat release system using the commercial code ANSYS ver. 13.0 and verified the validity of the new heat sink design for temperature distribution analysis.

关键词： LED lighting water-cooling heat release system heat loss

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Flapping dynamics of a flexible propulsor near ground

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Acta Mechanica Sinica 2016年第6期32卷 991-1000页

作者： Jaeha ryu sung Goon Park Boyoung Kim Hyung Jin sung Department of Mechanical Engineering Korea Advanced Institute of Science and Technology

The flapping motion of a flexible propulsor near the ground was simulated using the immersed boundary method. The hydrodynamic benefits of the propulsor near the ground were explored by varying the heaving frequency (St) of the leading edge of the flexible propulsor. Propulsion near the ground had some advantages in generating thrust and propelling faster than propulsion away from the ground. The mode analysis and flapping amplitude along the Lagrangian coordinate were examined to analyze the kinematics as a function of the ground proximity (d) and St. The trailing edge amplitude (\(a_\mathrm{tail}\)) and the net thrust (\(\overline{{F}}_x\)) were influenced by St of the flexible propulsor. The vortical structures in the wake were analyzed for different flapping conditions.

关键词： Flexible propulsor Ground effect Immersed boundary method Mode analysis

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Addition of cerium and yttrium to ferritic steel weld metal to improve hydrogen trapping efficiency

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International Journal of Minerals,Metallurgy and Materials 2017年第4期24卷 415-422页

作者： sung Jin Kim Kang Mook ryu Min-suk Oh Department of Advanced Materials Engineering Sunchon National University Jungang-ro Suncheon 540-742 Republic of Korea POSCO Steel Solution Marketing Division Geidong-Dong Pohang 790-704 Republic of Korea Automotive Components & Materials R&D Group Korea Institute of Industrial Technology 6 Cheomdan-gwagiro 208 beon-gil Gwangju 61012 Republic of Korea

The applicability of Ce and Y as promising candidate elements to form irreversible traps in weld metal was investigated by thermal desorption spectroscopy(TDS) with gas chromatography(GC). The precise nature of the precipitate particles newly formed in the weld metal by the addition of Ce and Y to a certain alloy system was characterized. Moreover,the hydrogen trapping efficiency expressed as the reduction of the diffusible hydrogen in the weld metal was analyzed. The results showed that the addition of Ce and/or Y to this alloy system led to the formation of a mixed type of(Ce,Ti)-based oxide,(Y,Ni)-based carbide,or(Ce,Y,Ti)-based oxide particles. Because of the high activation energy of the mixed type of particles(≥ 150 k J/mol),the trapping efficiency for hydrogen was considered to be sufficiently high to effectively reduce the diffusible hydrogen content.

关键词： hydrogen trapping weld rare earth metals cerium yttrium activation energy

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Unexpected complication during extracorporeal membrane oxygenation support: Ventilator associated systemic air embolism

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World Journal of Clinical Cases 2018年第9期6卷 274-278页

作者： Se-Min ryu sung-Min Park Department of Thoracic and Cardiovascular Surgery Kangwon National University Hospital School of Medicine Kangwon National Univerity

Systemic air embolism through a bronchovenous fistu-la(BVF) has been described in patients undergoing positive-pressure ventilation. However, no report has mentioned the potential risks of systemic air embolism through a BVF in patients undergoing extracorporeal membrane oxygenation(ECMO). Positive-pressure ventilation and ECMO support in patients with lung injury can increase the risk of systemic air embolism through a BVF. Increased alveolar pressure, decreased pulmonary venous pressure, and anticoagulation are thought to be the factors that contribute to this complication. Here, we present a case of systemic air embolism in a patient with ECMO and mechanical ventilator support.

关键词： Air embolism Extracorporeal membrane oxygenation Cerebral embolism Positive-pressure ventilation Cardio-pulmonary resuscitation

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Counter-balancing effects of Si on C partitioning and stacking fault energy of austenite in 10Mn quenching and partitioning steel

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Journal of Materials Science & Technology 2022年第3期98卷 248-257页

作者： Dong Hwi Kim Jee-Hyun Kang Hojun Gwon Joo Hyun ryu sung-Joon Kim Graduate Institute for Ferrous Technology Pohang University of Science and TechnologyPohang 37673Republic of Korea School of Materials Science and Engineering Institute of Materials TechnologyYeungnam UniversityGyeongsan 38541Republic of Korea POSCO Technical Research Laboratories POSCOGwangyang 57807Republic of Korea

Silicon is an essential alloying element in quenching and partitioning(Q&P)steels,because it is known to suppress carbide precipitation during partitioning step and promote carbon partitioning to stabilize *** 2 wt%Si was added to 10Mn-2Al-0.2C steel,the size and fraction of the carbides formed during partitioning became smaller than in the Si-free ***,the suppression of carbide formation promoted C partitioning into austenite as ***,austenite stability was always lower with Si under the equivalent partitioning condition because Si effectively decreased the stacking fault energy of *** partitioning progressed,the both yield and tensile strengths of the Si-added steel exceeded that of the Si-free steel with the similar ductility *** was because Si was an effective solid solution strengthener,and the austenite in the Si-added steel exhibited the appropriate stability to gradually transform into martensite throughout the *** resulting strengthening effect compensated for the softening caused by martensite ***,strain hardening rate decreased continuously throughout deformation,which resulted in high tensile strength and ductility.

关键词： Steels Q&P steel Silicon Austenite stability Tensile deformation Stacking fault energy Medium Mn steel

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Proteomic identification of cryostress in epididymal spermatozoa

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Journal of Animal Science and Biotechnology 2017年第2期8卷 478-489页

作者： sung Jae Yoon Md Saidur Rahman Woo sung Kwon Do Yeal ryu Yoo Jin Park Myung Geol Pang Department of Animal Science&Technology Chung-Ang UniversityAnseongGyeonggi-Do 456-756Republic of Korea

Background： Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation,thereby reducing sperm quality. We assessed the motility（%）, motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence ***, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis（2-DE） results using two commercial ***： Cryopreservation reduced viability（%）, motility（%）, straight-line velocity（VSL）, average path velocity（VAP）, amplitude of lateral head displacement（ALH）, and capacitated spermatozoa, whereas straightness（STR）and the acrosome reaction increased after cryopreservation（P 3 fold, P ＆lt; 0.05） before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation,and the guanylate cyclase ***： The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

关键词： Cryopreservation Cryostress Protein Spermatozoa

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Photosynthesis,Metabolite Composition and Anatomical Structure of Oryza sativa and Two Wild Relatives,*** and ***

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Rice science 2017年第4期24卷 218-227页

作者： Jwakyung sung Suyeon LEE Jong-Wook CHUNG Gerald EDWARDS Hojin ryu Taewan KIM National Institute of Agricultural Science Rural Development Administration(RDA)WanjuJeollabu-do 55365Korea Department of Industrial Plant Science and Technology Chungbuk National UniversityCheongju 28644Korea School of Biological Science Washington State UniversityPullmanWA 99164-4236USA Department of Biology Chungbuk National UniversityCheongju 28644Korea Department of Plant Resources and Science and Institute of Ecological Phytochemistry Hankyung National UniversityAnseong 17579Korea

Photosynthesis, enzyme activities and metabolite pools associated with primary carbon metabolism in leaves were studied in O. grandiglumis and O. alta （wild relatives of rice which produce high biomass） versus O. sativa （a japonica cultivar and a indica-japonica hybrid） to assess their potential for identifying traits which might be utilized to enhance rice productivity. The wild relatives had higher rates of photosynthesis on a fresh weight basis, and higher water use efficiency than the O. sativa lines. There were no striking differences in activities of a number of key enzymes in carbon and nitrogen metabolism between the wild relatives and cultivated rice lines. Along with higher rates of photosynthesis on a fresh weight basis, the leaves of the two wild species had higher nitrate content, higher levels of starch, glucose and fructose, and higher levels of organic acids （malate, succinate and acetate）, compared to the O. sativa lines. The results suggested that O. grandiglumis and O. alta have differences in physiology and primary metabolism which might be exploited to improve growth and productivity of cultivated rice.

关键词： carbohydrate photosynthesis rate nitrogen assimilation metabolite composition leaf rice wild relative

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On von Neumann Regularity of Commutators

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Algebra Colloquium 2024年第2期31卷 181-198页

作者： Nam Kyun Kim Tai Keun Kwak Yang Lee sung Ju ryu Nanqing Ding School of Basic Sciences Hanbat National UniversityDaejeon 34158Korea Department of Data Science Daejin UniversityPocheon 11159Korea Department of Mathematics Yanbian UniversityYanjiJilin 133002China Institute for Applied Mathematics and Optics Hanbat National University Daejeon 34158Korea Department of Mathematics Pusan National UniversityBusan 46241Korea 不详

We study the structure of rings which satisfy the von Neumann regularity of commutators,and call a ring R C-regularif ab-ba ∈(ab-ba)R(ab-ba)for all a,b in *** a C-regular ring R,we prove J(R[X])=N^(*)(R[X])=N^(*)(R)[X]=W(R)[X]■Z(R[X]),where J(A),N^(*)(A),W(A),Z(A)are the Jacobson radical,upper nilradical,Wedderburn radical,and center of a given ring A,respectively,and A[X]denotes the polynomial ring with a set X of commuting indeterminates over A;we also prove that R is semiprime if and only if the right(left)singular ideal of R is *** provide methods to construct C-regular rings which are neither commutative nor von Neumann regular,from any given ***,for a C-regular ring R,the following are proved to be equivalent:(i)R is Abelian;(ii)every prime factor ring of R is a duo domain;(ii)R is quasi-duo;and(iv)R/W(R)is reduced.

关键词： C-regular ring commutator regular ring commutative ring radical singular ideal

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Occipital artery bypass importance in unsuitable superficial temporal artery:Two case reports

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World Journal of Clinical Cases 2023年第9期11卷 2091-2097页

作者： Jong-Hwan Hong sung-Chan Jung Han-Seung ryu Tae-Sun Kim sung-Pil Joo Department of Neurosurgery Chonnam National University Hospital and Medical SchoolGwangju 61469South Korea

BACKGROUND Superficial temporal artery-middle cerebral artery(STA-MCA)bypass helps treat cerebral ***,the STA is not available for bypass in some ***,with some technical tips,the authors introduced a bypass technique using the occipital artery(OA).CASE SUMMARY Two female patients complained of *** magnetic resonance imaging(MRI)indicated contralateral infarction from the MCA *** Diamox single photon emission computed tomography or perfusion MRI,the contralateral front parietotemporal reserve was *** transfemoral cerebral angiography,the STA was thin with a weak flow;however,the OA was *** OA-MCA end-to-side extracranial-intracranial bypass surgery was implemented instead of STA because the caliber was too *** postoperative course was uneventful in both cases,with well-maintained bypass patency and neurological stability during *** OA might be an acceptable alternative for MCA cerebral ischemic cases with an unsuitable STA.

关键词： Bypass Occipital artery Superficial temporal artery Cerebral ischemia Case report

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