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Haemonchus contortus excretory and secretory proteins(HcESPs) suppress functions of goat PBMCs in vitro

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Haemonchus contortus excretory and secretory proteins(HcESPs...

中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： javaid ali gadahi Yongqian Bu Muhammad Ehsan Zhenchao Zhang Shuai Wang Ruofeng Yan Xiaokai Song Lixin Xu Xiangrui Li College of Veterinary Medicine Nanjing Agricultural University

Excretory and secretory products(ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products(Hc ESPs) were collected from the adult worms. Binding of Hc ESPs to goat peripheral blood mononuclear cells(PBMCs) was confirmed by immune-fluorescence assay. Effects of the Hc ESPs on cytokine production, cell proliferation, cell migration and nitric oxide(NO) production of PBMCs were checked by co-incubation of Hc ESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by Hc ESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by Hc ESPs, whereas, Hc ESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the Hc ESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.

关键词： Haemonchus contortus ESP goat PBMC immunomodulation

Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii 10kDa excretory-secretory antigen(TgESA10)

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Protective immunity against acute toxoplasmosis in BALB/c mi...

Nanoparticles(PLGA and Chitosan)-Entrapped ADP-Ribosylation Factor 1 of Haemonchus contortus Enhances the Immune Responses in ICR Mice

第五届全国人畜共患病学术研讨会

作者： Shuai Wang YuJian Wang XinChao Liu ZhengChao Zhang XiaoNi Sun javaid ali gadahi TingQi Liu Ibrahim A.Hassan LiXin Xu RuoFeng Yan XiaoKai Song Xiangrui Li 南京农业大学动物医学院

Toxoplasma gondii 10kDa excretory-secretory antigen(TgESAlO)is involved in the early stages of host *** aim of this study was to evaluate the immune protective efficacy of a DNA vaccine encoding TgESAlO gene against acute *** infection in *** gene sequence encoding TgESAlO was inserted into the eukaryotic expression vector pVAX1,and the efficacy of

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Nanoparticles(PLGA and Chitosan)-Entrapped ADP-Ribosylation ...

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Muhammad Waqqas Hasan Muhammad Haseeb Muhammad Ehsan javaid ali gadahi Muhammad ali-Ul-Husnain Naqvi Qiang Qiang Wang Xinchao Liu Shakeel Ahmed Lakho Ruofeng Yan Lixin Xu Xiaokai Song 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

ADP-ribosylation factor 1(HcARF1) is one of the Haemonchus contortus(***)excretory/secretory proteins involved in modulating the immune response of goat peripheral blood mononuclear cells(PBMC).Here,we evaluated the immunogenic potential of recombinant HcARF1(rHcARF1) against *** infection in Institute of Cancer Research(ICR) ***,rHcARF1 was entrapped in poly(D,L-lactide-co-glycolide)(PLGA) and chitosan(CS)nanoparticles(NP) and injected into mice as a ***-six ICR mice were assigned randomly into seven groups,with eight animals in each group,and they were vaccinated *** the end of the experiment(14 th day),the blood and the spleen were collected from euthanized mice to detect lymphocyte proliferation,cytokine analysis,and the production of antigen-specific *** electron microscope was used to determine the size,morphology,and zeta potential of *** cytometry was performed,which presented the increase percentages of CD4+T cells(CD3 e+CD4+),CD8+T cells(CD3 e+CD8+) and dendritic cells(CD11 c+CD83+,CD11 c+CD86+) in mice vaccinated with rHcARF1+PLGA *** analysis show raised humoral(Immunoglobulin(Ig)G1,IgG2 a,IgM) and cell-mediated immune response(Interleukin(IL)-4,IL-12,and IL-17,and Interferon(IFN)-γ)induced by rHcARFl+PLGA *** groups that were treated with the antigen-loaded NP yield higher lymphocyte proliferation than the control *** on these results,we could propose that the rHcARF1 encapsulated in NP could stimulate a strong immune response in mice rather than administering alone against the infection of ***.

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The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro

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The immunogenic maturation of goat monocyte-derived dendriti...

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Muhammad Waqqas Hasan Muhammad Haseeb Muhammad Ehsan javaid ali gadahi Qiangqiang Wang Muhammad ali Memon Muhammad Tahir Aleem Shakeel Ahmed Lakho Ruo Feng Yan Li Xin Xu Xiao Kai Song 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Previously,it was found that several proteins of Haemonchus contortus were involved in the stimulation of the host immune ***,the information about the selection of superlative antigens with immunogenic efficacies on host DCs is *** the current study,the stimulatory effects of five recombinant proteins(elongation factor-1α,arginine kinase,ES-15,ES-24,and ADP-ribosylation factor 1) of *** on the maturation of goat monocyte-derived dendritic cells(md-DCs) were *** proteins were purified separately in *** expression and incubated with isolated goat peripheral blood mononuclear cells(PBMC).Immunofluorescence assay(IFA) results confirmed the binding of these molecules to the md-DC’s surface as compared to control *** the flow cytometry analysis,recombinant proteins induced md-DC stimulation via the up-regulation of the expression of the costimulatory molecule(CD80) and MHC-Ⅱ.Quantitative RT-PCR data showed a significant increase in the expression of specific genes of the WNT and toll-like receptor(TLR) signaling *** result of ELISA indicated the higher levels of cytokine(IL-10,IL-12,IFN-y,and TNF-α) secretion in the md-DC compared to the negative(pET-32 a His-Tag) and blank(PBS)control *** data gives valuable support in the selection of potential antigens for future studies on the immunomodulation of the host against the infection of ***.

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Ultrastructural Changes of Scolex on Cysticercus pisiformis during Relative Rest and Motion States

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Agricultural Science & Technology 2014年第1期15卷 111-115页

作者：潘耀谦张柳平王帅李瑞珍刘志科孙玉倩 javaid ali gadahi 刘兴友河南科技学院动物科学学院河南新乡453003 江苏大学医学院江苏镇江212013 南京农业大学动物医学学院江苏南京210095 信德农业大学/兽医寄生虫学系

[Objective] This study aimed to research the ultra-morphological changes of scolex of Cysticercus pisiformis during the relative rest and motion states. [Method] The ultrastructure changes of scolex located in cyst and evaginated from cyst after cultivation were comparatively observed by scanning electron microscope. [Result] When the scolex was in the relative rest state, observed from the top, the rostel um with the tegument muscular column that connected to tooth-hook looked like the umbrel a and covered on the front end of the scolex. Viewed from the side of the scolex, the tooth-hook on the rostel um looked like the antler branch and had only one row. Four suckers looked like cavities, and were located in the back of the rostel um and distributed around the scolex in the equidistance. When the scolex was in the motion states, the tegument muscular column on the rostel um contracted, the antler-like tooth-hook extended to periphery, and the sucker also made the ring-like and longitudinal-like contraction. [Conclusion] Ultrastructure of the scolex of C. pisiformis changed apparently during relative rest and motion states. Those changes help scolex to invade the host tissue.

关键词： Cysticercus pisiformis Scolex Rostellum Sucker Ultrastructure

Heat Transfer Enhancement of an Automobile Engine Radiator using ZnO Water Base Nanofluids

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Journal of Thermal Science 2020年第4期29卷 1010-1024页

作者： Muhammad QASIM Muhammad SAJID KAMRAN Muhammad AMMAR Muhammad ali JAMAL Muhammad YASAR javaid Department of Mechanical Engineering University of Engineering and TechnologyLahore 54890Pakistan Department of Chemical Engineering Technology Government College UniversityFaisalabad 38000Pakistan Department of Mechanical Engineering Technology Government College UniversityFaisalabad 38000Pakistan

In this research paper, the forced convective heat transfer enhancement of a Suzuki Mehran(VXR) 2016 radiator(heat exchanger) along with pressure drop and friction factor by utilizing Zinc oxide(Zn O) water based nanofluids has been experimentally studied. Three types of nanofluids with different volumetric concentrations of Zn O nanoparticles(0–0.3%) were employed in order to understand its effect on heat transfer enhancement. The experimental setup was completely designed as closely as possible to the car cooling system. The experimentation has been done under laminar flow conditions(186≤Re≤1127) at different fluid volume flow rates(2–12 L/min) and constant fluid inlet temperature(70°C) to the automobile radiator. A maximum enhancement in heat transfer rate, overall heat transfer coefficient and Nusselt number was obtained up to 41%, 50% and 31% by using nanofluid with 0.2% volumetric concentration of nanoparticles respectively. On the other hand, the mean enhancement in pressure drop and friction factor was obtained up to 47% and 46% by using nanofluid with the same volumetric concentration of nanoparticles i.e. 0.2% respectively. The experimental results also revealed that the heat transfer rate, overall heat transfer coefficient and Nusselt number of nanofluids increases by increasing the volume flow rates and volumetric concentration of nanoparticles. However, these thermal performance parameters of nanofluids started to decline when the volumetric concentration of nanoparticles was increased from 0.2% to 0.3%. Furthermore, pressure drop and friction factor of nanofluids increase by increasing the volumetric concentration of nanoparticles, while pressure drop increases and friction factor decreases by increasing the volume flow rate of nanofluids respectively. At the end, the thermal efficiency of automobile radiator with high cooling rates was obtained by using nanofluid with 0.2% volumetric concentration of nanoparticles.

关键词： ZnO nanoparticles heat transfer enhancement thermal efficiency automobile radiator

Braids with Trivial Simple Centralizer

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Algebra Colloquium 2015年第4期22卷 561-566页

作者： U. ali F. Azam I. javaid A. Haider Center for Advanced Studies in Pure and Applied Mathematics Bahauddin Zakariya University Multan Pakistan Department of Mathematics Jazan University Jazan Saudi Arabia

In this paper, we prove that the n-simple braid divisible by the generators xi for all 2 ≤ i ≤n - 2 has trivial simple centralizer. Consequently, the commuting graph defined on the set of simple braids is disconnect... 详细信息

关键词： simple braid simple centralizer commuting graph

The charge state distribution of B, C, Si, Ni, Cu and Au ions on 5 MV pelletron accelerator

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Nuclear Science and Techniques 2017年第5期28卷 43-47页

作者： ali Awais javaid Hussain Muhammad Usman Waheed Akram Kashif Shahzad Turab ali Ishaq Ahmad Malik Maaza Experimental Physics Laboratories National Centre for Physics Islamabad 44000 Pakistan UNESCO-UNISA Africa Chair in Nanosciences/ Nanotechnology College of Graduate Studies University of South Africa Muckleneuk Ridge PO Box 392 PretoriaSouth Africa Nanosciences African Network (NANOAFNET) iThemba LABS-National Research Foundation 1 Old Faure RoadPO Box 722 Somerset West Western Cape Province 7129South Africa

Stripper gas and terminal potential play a key role for the charge state distribution in a tandem pelletron accelerator. The knowledge of this distribution is important for experiments performed on tandem accelerators. The charge state distribution of B, C, Si, Ni, Cu and Au beams is measured by using Ar as stripper gas, and terminal potential is varied from 0.3 to 3.0 MV on 5UDH-2 tandem pelletron accelerator installed at the National Centre for Physics, Islamabad. The individual charge state is measured after the switching magnet at 15° in high-energy portion. It is observed that the higher charge states are stable in the range of lower and middle atomic masses of periodic table, whereas higher atomic mass(Au) shows beam current instability in higher charge states. For carbon,the charge distribution at 1.7 MV terminal potential by varying stripper gas pressure is also studied, which resulted in decreased overall transmission with good current value for higher charge states.

关键词： Pelletron accelerator Charge state distribution Stripper gas Terminal potential

Hydrothermal synthesis and characterization of carbon-doped TiO2 nanoparticles

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Chinese Physics B 2020年第11期29卷 516-521页

作者： Zafar ali javaid Ismail Rafaqat Hussain AShah Arshad Mahmood Arbab Mohammad Toufiq Shams ur Rahman Department of Physics COMSATS University IslamabadPark RoadIslamabad 45550Pakistan Department of Chemistry COMSATS University IslamabadPark RoadIslamabad 45550Pakistan National Institute of Lasers and Optronics College Pakistan Institute of Engineering and Applied Sciences(NILOP-CPIEAS)Nilore 45650IslamabadPakistan Department of Physics Hazara University MansehraMansehra 21300Pakistan

We report the hydrothermal growth of pure and doped TiO2 nanoparticles with different concentrations of *** microstructure of the as-synthesized samples is characterized by x-ray diffraction(XRD),field emission scanning electron microscopy(FESEM),energy dispersive x-ray spectroscopy(EDX),and Raman spectroscopy to understand the structure and *** XRD patterns confirm the formation of anatase phase of TiO2 with the average crystallite size is calculated to be in the range of 13 nm to 14.7 *** functional groups of these nanostructures are characterized by Fourier transformed infrared(FT-IR)spectroscopy,which further confirms the single anatase phase of the synthesized ***-visible absorption spectroscopy is used to understand the absorption behavior,which shows modification in the optical bandgap from 3.13 eV(pure TiO2)to 3.74 eV(1.2 mol%C-doped TiO2).Furthermore,the Ti^3+centers associated with oxygen vacancies are identified using electron paramagnetic resonance spectroscopy(EPR).

关键词： titanium dioxide hydrothermal synthesis defect states bandgap