Haemonchus contortus excretory and secretory proteins(HcESPs) suppress functions of goat PBMCs in vitro
作者单位：College of Veterinary Medicine Nanjing Agricultural University
会议名称：《中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会》
会议日期：2016年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：Haemonchus contortus ESP goat PBMC immunomodulation
摘 要：Excretory and secretory products(ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products(Hc ESPs) were collected from the adult worms. Binding of Hc ESPs to goat peripheral blood mononuclear cells(PBMCs) was confirmed by immune-fluorescence assay. Effects of the Hc ESPs on cytokine production, cell proliferation, cell migration and nitric oxide(NO) production of PBMCs were checked by co-incubation of Hc ESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by Hc ESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by Hc ESPs, whereas, Hc ESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the Hc ESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.