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Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

Genes & Diseases 2023年第5期10卷 2137-2150页

作者： Yunyi Liu Juan Li Hailong Ou Dan Qi Bei Hu Yuxi Xu Jian Hu Yi Xiong Luling Xia jason hhuang Xiaoxiao Hu Erxi Wu State Key Laboratory of Chemo/Biosensing and Chemometrics College of BiologyMolecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082China Shenzhen Research Institute Hunan UniversityShenzhenGuangdong 518000China Department of Neurosurgery and Neuroscience Institute Baylor Scott&White HealthTempleTX 76508USA Department of Respiratory Medicine The Third Xiangya HospitalCentral South UniversityChangshaHunan 410013China Texas A&M University School of Medicine College StationTX 77843USA Texas A&M University School of Pharmacy College StationTX 77843USA LIVESTRONG Cancer Institutes and Department of Oncology Dell Medical SchoolThe University of Texas at AustinAustinTX 78712USA Greater Bay Area Institute for Innovation Hunan UniversityGuangzhouGuangdong 511300China

Aptamers,short single DNA or RNA oligonucleotides,have shown immense application potential as molecular probes for the early diagnosis and therapy of ***,conventional cell-SELEX technologies for aptamer discovery are time-consuming and *** we discovered a new aptamer BC-3 by using an improved rapid X-Aptamer selection process for human bladder carcinoma,for which there is no specific molecular probe *** show that BC-3 exhibited excellent affinity in bladder cancer cells but not normal *** demonstrate that BC-3 displayed high selectivity for tumor cells over their normal counterparts in vitro,in mice,and in patient tumor tissue *** endocytosis pathway analysis revealed that BC-3 internalized into bladder cancer cells via clathrin-mediated ***,we identified ribosomal protein S7(RPS7)as the binding target of BC-3 via an integrated methodology(mass spectrometry,colocalization assay,and immunoblotting).Together,we report that a novel aptamer BC-3 is discovered for bladder cancer and its properties in the disease are *** findings will facilitate the discovery of novel diagnostic and therapeutic strategies for bladder cancer.

关键词： Bladder cancer Cell-SELEX Clathrin-mediated endocytosis Intracellular colocalization Ribosomal protein S7 X-aptamer selections

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Astrocytic modulation of potassium under seizures

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Neural Regeneration Research 2020年第6期15卷 980-987页

作者： Fushun Wang Xiaoming Qi Jun Zhang jason hhuang Department of Neurosurgery Baylor Scott&White HealthTempleTXUSA Institute of Brain and Psychological Science Sichuan Normal UniversityChengduSichuan ProvinceChina Department of Neurosurgery PLA General HospitalBeijingChina Department of Surgery Texas A&M University College of MedicineTempleTXUSA

The contribution of an impaired astrocytic K^+ regulation system to epileptic neuronal hyperexcitability has been increasingly recognized in the last decade.A defective K^+ regulation leads to an elevated extracellular K^+ concentration([K^+]o).When[K^+]o reaches peaks of 10-12 mM,it is strongly associated with seizure initiation during hypersynchronous neuronal *** the other hand,reactive astrocytes during a seizure attack restrict influx of K^+ across the membrane both passively and *** addition to decreased K^+ buffering,aberrant Ca^2+ signaling and declined glutamate transport have also been observed in astrogliosis in epileptic specimens,precipitating an increased neuronal discharge and induction of *** review aims to provide an overview of experimental findings that implicated astrocytic modulation of extracellular K^+ in the mechanism of epileptogenesis.

关键词： AQP4 Ca^2+ signaling cell volume connexins epilepsy K^+ buffering mi RNA NKCC pannexin TBI

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