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Virome identification in wheat in the Czech Republic using small RNA deep sequencing

Journal of Integrative Agriculture 2020年第7期19卷 1825-1833页

作者： Khushwant SINGH jana jarosova Jan FOUSEK CHEN Huan Jiban Kumar KUNDU Plant Virus and Vector Interactions Group Division of Crop Protection and Plant HeatthCrop Research InstitutePrague 16106Czech Republic Zhejiang Tianke High-Technology Development Co. Ltd.Hangzhou 310012P.R.China

High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA(sRNA)population isolated from plants allows universal virus detection and complete virome reconstruction for a given *** the present sRNA deep-sequencing analysis of virus-infected wheat samples in the Czech Republic,samples were firstly tested for barley yellow dwarf viruses(BYDVs),wheat streak mosaic virus(WSMV)and wheat dwarf virus(WDV)using ELISA,RT-PCR and *** sRNA sequencing of these samples yielded more than^60 million single-end 50-bp reads with high confidence for nine field samples of ***,16.5%of reads were virus-specific and 83.5%were mapped to the *** 21-nt reads(-7.7E+06 reads)were found than 24-nt(~6.20E+06 reads)or 22-nt(-4.30E+06 reads)*** novo assembly of the high-quality contigs revealed the presence of three earlier repoted viruses in the Czech Republic:BYDVs(31.48%),WSMV(24.23%)and WDV(2666%).We also showed the presence of cereal yellow dwarf virus(14.33%;two species CYDV-RPS and CYDV-RPV family Luteoviridae/Polerovirus)and wheat yellow dwarf virus(WYDV,3.30%;Luteoviridae).Phylogenetic analysis showed CYDV and WYDV grouped separately from ***,several recombination breakpoints were found among the groups of yellow dwarf viruses(BYDVs,CYDV,and WYDV).Using RNA deep sequencing,we confirmed the presence of the three known viruses(BYDVs,WSMV,and WDV)and the first record of two species of CYDV and WYDV in wheat in the Czech Republic.

关键词： NGS sRNA BYDVs CYDV WSMV WYDV WDV

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Endoscopic radiofrequency ablation for malignant biliary obstruction

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World Journal of Gastrointestinal Oncology 2021年第10期13卷 1383-1396页

作者： jana jarosova Peter Macinga Alzbeta Hujova Jan Kral Ondrej Urban Julius Spicak Tomas Hucl Department of Gastroenterology and Hepatology Institute for Clinical and Experimental MedicinePraha 14021Czech Republic Department of Internal Medicine II–Gastroenterology and Geriatrics University Hospital OlomoucFaculty of Medicine and DentristryPalacky University OlomoucOlomouc 77900Czech Republic

Cholangiocarcinoma and pancreatic cancer are the most common causes of malignant biliary *** majority of patients are diagnosed at a late stage when surgical resection is rarely *** these cases,palliative chemotherapy and radiotherapy provide only limited benefit and are associated with poor *** ablation(RFA)is a procedure for locoregional control of tumours,whereby a high-frequency alternating current turned into thermal energy causes coagulative necrosis of the tissue surrounding the *** subsequent release of debris and tumour antigens by necrotic cells can stimulate local and systemic *** development of endoluminal RFA catheters has led to the emergence of endoscopically delivered RFA,a treatment mainly used for malignant biliary strictures to prolong survival and/or stent *** indications include recanalisation of occluded biliary stents and treatment of intraductal ampullary adenoma or benign biliary *** article presents a comprehensive review of endobiliary RFA,mainly focusing on its use in patients with malignant biliary *** available data suggest that biliary RFA may be a promising modality,having positive impacts on survival and stent patency and boasting a reasonable safety ***,further studies with better characterised and stratified patient populations are needed before the method becomes accepted within routine clinical practice.

关键词： Radiofrequency Ablation Biliary Stenosis Cholangiocarcinoma Pancreatic cancer

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Biliary obstruction following transjugular intrahepatic portosystemic shunt placement in a patient after liver transplantation:A case report

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World Journal of Hepatology 2022年第5期14卷 1038-1046页

作者： Peter Macinga Darina Gogova Jan Raupach jana jarosova Libor Janousek Eva Honsova Pavel Taimr Julius Spicak Jiri Novotny Jan Peregrin Tomas Hucl Department of Gastroenterology and Hepatology Institute for Clinical and Experimental MedicinePrague 14021Czech Republic Department of Radiology University HospitalHradec Kralove 50005Czech Republic Department of Transplant Surgery Institute for Clinical and Experimental MedicinePrague 14021Czech Republic Department of Pathology Institute for Clinical and Experimental MedicinePrague 14021Czech Republic Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental MedicinePrague 14021Czech Republic

BACKGROUND Transjugular intrahepatic portosystemic shunt(TIPS)is a method used to decrease portal *** stricture is the rarest of the complications associated with this procedure with only 12 cases previously reported in the *** of these cases have documented the resolution of biliary stenosis induced by a stent *** only curative solutions reported are liver transplantation or bypassing the stenosis with an artificial biliary tract using advanced endoscopic *** SUMMARY This is the first reported case of biliary obstruction secondary to TIPS placement in a transplanted *** our patient,a portosystemic shunt was created to treat severe veno-occlusive liver graft disease manifesting itself primarily by fluid retention.A cholestatic liver lesion and cholangitis with abscesses developed due to a stent graft-induced stricture in the dorsal segment of the right hepatic duct and the stricture diminished following percutaneous *** drainage was performed after unsuccessful removal of the percutaneous catheter resulting in a bilio-cutaneous *** the liver graft now functions well,the stricture remains refractory even after 44 mo of *** Biliary strictures caused by TIPS in both transplanted and native livers seem refractory to endoscopic treatment.

关键词： Biliary stricture Transjugular intrahepatic portosystemic shunt Liver trans-plantation Sinusoidal obstruction syndrome Literature review Case report

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