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Impact of anthropogenic pressure on the formation of population structure and genetic diversity of raccoon dog Nyctereutes procyonoides

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Current Zoology 2016年第5期62卷 413-420页

作者： Loreta GRICIUVIENE Algimantas PAULAUSKAS jana RADZIJEVSKAJA Judita ZUKAUSKIENE Irma PURAITE Faculty of Natural Sciences Department of Biology Vytautas Magnus University Vileikos str. 8 Kaunas LT-44404Lithuania

The raccoon dog Nyctereutes procyonoides experienced an active introduction and acclimatization in the European part of Russia followed by its migration to and colonization in the neighboring countries. Eventually, it has spread rapidly into many European countries. N. procyonoides probably invaded Lithuania from the neighboring countries of Belarus and Latvia where the species was introduced. However, the data on genetic diversity and population structure of the raccoon dogs in the recently invaded territories are still scarce. The objectives of this study were to investigate genetic diversity of N. procyonoides in Lithuania after acclimatization, and to assess the impact of anthropogenic pressure on the formation of population structure. A total of 147 N. procyonoides individuals collected from different regions of Lithuania were genotyped using 17 microsatellite markers. The microsatellite analysis of raccoon dogs indicated high levels of genetic diversity within the population. The Bayesian clustering analysis in STRUCTURE identified 4 genetic clusters among sampled raccoon dogs that could not reveal a clear separation between subpopulations. The widespread distribution of raccoon dogs in Lithuania, high level of genetic variation observed within subpopulations, and low level of variation portioned among subpopulations suggest migration and gene flow among locations. The significant correlation between genetic and geographic distances indicated isolation that reflected the distance between locations. The fencing of highways and very intensive traffic could be barriers to gene flow between the western and eastern sampling areas of raccoon dogs.

关键词： Nyctereutes procyonoides microsatellite loci invasion genetic diversity Lithuania.

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Sexual dimorphism in the alpine butterflies Boloria pales and Boloria napaea:differences in movement and foraging behavior (Lepidoptera:Nymphalidae)

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Insect Science 2018年第6期25卷 1089-1101页

作者： Stefan Ehl Katharina Hostert jana Korsch Patrick Gros Thomas Schmitt Department of Biogeography University of TrierTrierGermany Haus der Natur Salzburg Work group EntomologySalzburgAustria Senckenberg German Entomological Institute MünchebergGermany Entomology Department of ZoologyInstitute of Biology Martin Luther University Halle-WittenbergHalle (Saale)Germany

Sexual dimorphism is a widespread phenomenon in *** is reflected in differences in life history,behavior and *** of differences in behavior are mostly difficult and time-consuming,especially in high mountain *** enhance our knowledge on sexual dimorphisms of alpine butterflies,we performed a mark-release-recapture study on 2 species common in the Alps:Boloriapales and Boloria *** analysed movement and foraging behavior to investigate differences between *** sexes were mostly sedentary and the movement distances of males and females ***,obvious differences in dispersal behavior between the sexes were found in the movement *** different patterns were *** males showed intensive flight activity,but mostly flew only in a limited part of the entire habitat (i.e.,their individual home range)searching for females,whereas females were less flight active and flew only to find places for oviposition or *** third pattern,where individuals flew larger distances,was only observed in a small number of males,which always returned to their home *** all feeding was observed on ***,males preferred the genera Leontodon and Crepis,while females preferred Leontodon and *** from this sexual difference in foraging,individuals of both sexes were found to be more or less specialised on nectar *** activity was generally greater in males than ***,we think that sex-specific requirements in nectar ingredients exist,that is,sugar for the intensive flight activity of males and amino acids for egg production of females.

关键词： foraging behavior mark-release-recapture movement patterns nectar source specialization sexual dimorphism

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Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format

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Journal of Bionic Engineering 2008年第4期5卷 291-298页

作者： Anett Reichert jana Felbel Mark Kielpinski Matthias Urban Benno Steinbrecht Thomas Henkel Microfluidics Department Institute of Photonic Technology Arvato Digital Services Manufacturing EMEA

Chip-based flow-through PCR implements the PCR as a continuous process for nucleic acid analytics. The sample is transported in a winding channel through temperature zones required for denaturation, annealing and extension. Main fields of application are the monitoring of continuous processes for rapid identification of contaminants and quality control as well as high throughput screening of cells or microorganisms. A modular arrangement with five heating zones for flow-through PCR is discussed and evaluated. The special heater arrangement allows the implementation of up to 40 cycles on the footprint of a microscope slide, which is placed on top ofa 5 zones heating plate. Liquid/liquid two phase flow of PCR reaction mixture and mineral oil have been applied to create a segmented flow process scheme. In that way, the developed system may provide flow-through PCR as a unit operation for the droplet based microfluidics platform. The single use of disposable devices is commonly preferred due to the sensitivity of the PCR process to contaminations. All-glass microfluidic chips and disposable chip devices, made from polycarbonate as a replication with identically geometry, have been fabricated and tested. For the first time, microchannel geometries with nearly circular profile developed by all-glass technology have been transferred to mass fabrication by injection compression molding. Both devices have been successfully applied for the detection of the tumor suppressor gene p53. Although product yield and selectivity of the amplification process do not depend on the chip material, a well defined, reliable segmented flow regime could only be realized in the all-glass chip.

关键词： lab-on-a-chip PCR microfluidics two-phase-flow injection molding point-of-care biomedical

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Interplay between reproduction and age selective harvesting:A case study of Hilsa(Tenualosa ilisha)fish at Sundarban estuary of northern Bay of Bengal,India

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International Journal of Biomathematics 2019年第2期12卷 239-260页

作者： Debaldev jana Sachinandan Dutta G.P.Samanta Department of Mathematics&SRM Research Institute SRM Institute of Science and Technology Kattankulathur 603 203 Tamil NaduIndia Aquatic Bioresource Research Laboratory Department of Zoology University of Calcutta 35 Ballygunge Circular RoadKolkata 700 019India Department of Mathematics Indian Institute of Engineering Science and Technology Shibpur Howrah 711103India

New offspring due to sexual reproduction is not an instantaneous process from its birth,it takes time to be sexually *** the other hand,harvesting of commercially profitable fish population before the perfect size or weight is reached is not only a commercial loss but also risks the extinction of the ***,we discuss the issue of Ganges-Brahmaputra-Meghna basin,northern Bay of Bengal for the age-selective harvesting of Hilsa shad(Tenualosa ilisha)which lays eggs after its sexual *** of hilsa before its sexual maturation risks its extinction and due to lamer body weight,it is not a commercially profitable *** is a reality of Sundarban estuary for hilsa fish harvesting,therefore,biologically and economically both India and Bangladesh are facing several *** data of Frasergunje Fishing Harbor shows a clear picture as the supporting document of this mathematical problem.

关键词： Maturation delay age selective harvesting extinction risk MSY bionomic equilibrium optimal harvesting

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Numerical simulation of fluidized dense-phase pneumatic conveying of powders to develop improved model for solids friction factor

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Particuology 2017年第6期15卷 42-50页

作者： Baldeep Kaur Anu Mittal S.S. Mallick Renhu Pan Soumendu jana School of Physics and Materials Science Thapar University Patiala Punjab 147004 India Department of Mechanical Engineering Thapar University Patiala Punjab 147004 India Fujian Longking Co. Ltd. Longyan Fujian 364000 China

Accurate prediction of the solids friction factor through horizontal straight pipes is important for the reliable design of a pneumatic conveying system, but it is a challenging assignment to date because of the highly concentrated, turbulent, and complex nature of the gas-solids mixture. Power-station fly ash was transported through different pipeline configurations. Numerical simulation of the dense-phase pneumatic conveying systems for three different solids and two different air flow rates have shown that particle and actual gas velocities and the ratio of the two velocities increases in the flow direction, whereas the reverse trend was found to occur for the solids volumetric concentration. To develop a solids friction-factor model suitable for dense-phase flow, we modified an existing pure dilute-phase model by incorporating sub-models for particle and actual gas velocities and impact and solids friction factor. The solids friction-factor model was validated by using it for scale-up predictions for total pipeline pressure drops in longer and larger pipes and by comparing experimental and predicted pneumatic conveying characteristics for different solids flow rates. The accuracy of the prediction was compared with a recently developed two-layer-based model. We discussed the effect of incorporating the particle and actual gas velocity terms in the solids friction-factor model instead of superficial air velocity.

关键词： Pneumatic conveying Dense-phase Numerical analysis Pressure drop Scale-up validation

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The impact of preozonation on the coagulation of cellular organic matter produced by Microcystis aeruginosa and its toxin degradation

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Journal of Environmental Sciences 2020年第12期32卷 124-133页

作者： Magdalena Barešová jana Naceradská Katerina novotná LenkaCermáková Martin Pivokonský Institute of Hydrodynamics of the Czech Academy of Sciences Pod Patankou 516612 Prague 6Czech Republic

Ozonation pretreatment is typically implemented to improve algal cell coagulation. However, knowledge on the effect of ozonation on the characteristics and coagulation of associated algal organic matter, particularly cellular organic matter(COM), which is extensively released during algal bloom decay, is limited. Hence, this study aimed to elucidate the impact of ozonation applied before the coagulation of dissolved COM from the cyanobacteria Microcystis aeruginosa. Additionally, the degradation of microcystins(MCs) naturally present in the COM matrix was investigated. A range of ozone doses(0.1–1.0 mg O3/mg of dissolved organic carbon – DOC) and ozonation pH values(pH 5, 7 and 9) were tested, while aluminium and ferric sulphate coagulants were used for subsequent coagulation. Despite negligible COM removal, ozonation itself eliminated MCs, and a lower ozone dose was required when performing ozonation at acidic or neutral pH(0.4 mg O3/mg DOC at pH 5 and 7 compared to 0.8 mg O3/mg DOC at pH 9). Enhanced MC degradation and a similar pattern of pH dependence were observed after preozonation-coagulation, whereas coagulation alone did not sufficiently remove MCs. In contrast to the benefits of MC depletion, preozonation using ≥0.4 mg O3/mg DOC decreased the coagulation efficiency(from 42%/48% to 28%–38%/41%–44% using Al/Fe-based coagulants), which was more severe with increasing ozone dosage. Coagulation was also influenced by the preozonation pH, where pH 9 caused the lowest reduction in COM removal. The results indicate that ozonation efficiently removes MCs, but its employment before COM coagulation is disputable due to the deterioration of coagulation.

关键词： Algal organic matter Coagulation Microcystins Microcystis aeruginosa Ozonation

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Virome identification in wheat in the Czech Republic using small RNA deep sequencing

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Journal of Integrative Agriculture 2020年第7期19卷 1825-1833页

作者： Khushwant SINGH jana JAROSOVA Jan FOUSEK CHEN Huan Jiban Kumar KUNDU Plant Virus and Vector Interactions Group Division of Crop Protection and Plant HeatthCrop Research InstitutePrague 16106Czech Republic Zhejiang Tianke High-Technology Development Co. Ltd.Hangzhou 310012P.R.China

High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA(sRNA)population isolated from plants allows universal virus detection and complete virome reconstruction for a given *** the present sRNA deep-sequencing analysis of virus-infected wheat samples in the Czech Republic,samples were firstly tested for barley yellow dwarf viruses(BYDVs),wheat streak mosaic virus(WSMV)and wheat dwarf virus(WDV)using ELISA,RT-PCR and *** sRNA sequencing of these samples yielded more than^60 million single-end 50-bp reads with high confidence for nine field samples of ***,16.5%of reads were virus-specific and 83.5%were mapped to the *** 21-nt reads(-7.7E+06 reads)were found than 24-nt(~6.20E+06 reads)or 22-nt(-4.30E+06 reads)*** novo assembly of the high-quality contigs revealed the presence of three earlier repoted viruses in the Czech Republic:BYDVs(31.48%),WSMV(24.23%)and WDV(2666%).We also showed the presence of cereal yellow dwarf virus(14.33%;two species CYDV-RPS and CYDV-RPV family Luteoviridae/Polerovirus)and wheat yellow dwarf virus(WYDV,3.30%;Luteoviridae).Phylogenetic analysis showed CYDV and WYDV grouped separately from ***,several recombination breakpoints were found among the groups of yellow dwarf viruses(BYDVs,CYDV,and WYDV).Using RNA deep sequencing,we confirmed the presence of the three known viruses(BYDVs,WSMV,and WDV)and the first record of two species of CYDV and WYDV in wheat in the Czech Republic.

关键词： NGS sRNA BYDVs CYDV WSMV WYDV WDV

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Inhibiting Protein Aggregation by Small Molecule-Based Colloidal Nanoparticles

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Accounts of Materials Research 2022年第1期3卷 54-66页

作者： Koushik Debnath Ankan Kumar Sarkar Nihar R.jana Nikhil R.jana Department of Pharmacology and Regenerative Medicine College of MedicineUniversity of Illinois at ChicagoChicagoIllinois 60612United States Department of Biomedical Engineering College of MedicineUniversity of Illinois at ChicagoChicagoIllinois 60607United States School of Materials Sciences Indian Association for the Cultivation of ScienceKolkata 700032India

CONSPECTUS:Protein aggregation is associated with different human diseases such as Alzheimer’s,Huntington’s,Parkinson’s,diabetes type II,and *** no effective treatment exists for many of these diseases,particularly for neurological *** research focuses on understanding the origin of protein aggregation,nucleation−growth mechanism of protein aggregation,origin of cytotoxicity of protein aggregates,cellular response of toxic protein aggregates,progress of diseases at intra/extracellular space,and drug developments for respective *** issues are the identification of molecular drugs that can inhibit protein aggregation at early stage,lowering of toxicity due to protein aggregates,delivery of drugs to remote organ and intracellular space,clearing matured protein aggregates from cell/extracellular space/brain,and design of effective therapeutic *** and materials scientists have identified a wide variety of antiamyloidogenic small molecules,macromolecules,and *** is shown that antiamyloidogenic molecules prevent protein oligomerization via binding to protein,masking metal ions(via chelating with metal ions)that are responsible for protein aggregation via generating reactive oxygen species(ROS),and lowering protein−protein interaction via macromolecular crowding ***,nanoscale materials with curved surface and multiple chemical functional groups act as adsorption/binding sites of proteins,modulate nucleation−growth kinetics of protein aggregation,and delay/inhibit protein aggregation in many ***,performance of all these antiamyloidogenic materials needs significant improvement,and proper therapeutic strategies are required for effective drug *** this Account,we describe that the performance of antiamyloidogenic molecules can be greatly improved via appropriate design into colloidal and nanoparticle *** first discuss different human diseases that are linked with protein agg

关键词： integrating Huntington curved

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Multi-fluid modeling of density segregation in a dense binary fluidized bed

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Particuology 2012年第1期10卷 62-71页

作者： Zhongxi Chao Yuefa Wang jana P. Jakobsen Maria Fernandino Hugo A. Jakobsen Department of Chemical Engineering NTNU Trondheim 7491 Norway SINTEF Energy Trondheim 7491 Norway Department of Energy and Process Engineering NTNU Trondheim 7491 Norway

This paper presents simulation results of the density segregation in a dense binary gas fluidized bed using a multi-fluid model from Chao et al. （2011）, The segregation behavior of two types of particles with approximately same particle diameters and different particle densities was studied and validated using the experimental data from Formisani et al, （2008）. Some detailed information regarding the gas, particle velocity profiles, the distributions of the particle volume fractions and the flotsam-to-total par- ticle volume fraction ratios is presented. The simulation results show that the simulated axial average flotsam-to-total particle volume fraction ratio distribution agrees reasonably with the experimental data of Formisani et al. （2008）. The binary particle velocities are closely coupled though the segregation exists, The segregation behavior and the particle velocity profiles are superficial gas velocity dependent. The number and distribution of particle velocity vortices change dramatically with superficial gas velocity： at a comparatively low superficial gas velocity, the particles mainly segregate axially, and at a comparatively high superficial gas velocity, the particles segregate both axially and radially.

关键词： Fluidized bedBinary flowDensity segregationMulti-fluid modelGranular flow

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CD133 and membrane microdomains:Old facets for future hypotheses

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World Journal of Gastroenterology 2011年第36期17卷 4149-4152页

作者： Christine A Fargeas jana Karbanová József Jászai Denis Corbeil Tissue Engineering Laboratories (BIOTEC) Technische Universitt DresdenD-01307 DresdenGermany

Understanding all facets of membrane microdomains in normal and cancerous cells within the digestive tract is highly important,not only from a clinical point of view,but also in terms of our basic knowledge of cellular *** studying the normal and cancer stem cell-associated molecule CD133 (prominin-1),novel aspects of the organization and dynamics of polarized epithelial cells have been revealed during the last *** association with particular membrane microdomains is highly relevant in these contexts and might also offer new avenues in diagnosis and/or targeting of cancer stem cells.

关键词： AC133 Cancer CD133 Membrane microdomains Membrane vesicles Prominin-1 Stem cell

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