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Molecular Cloning and Characterization of a Candidate ENOX Protein of <i>Saccharomyces cerevisiae</i>with a 25 Min Period Insensitive to Simalikalactone D Inhibition and Melatonin

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Advances in Biological Chemistry 2014年第5期4卷 339-350页

作者： Aya F. Ryuzoji Debby H. Parisi Sara Dick james kim Dorothy M. Morré D. james Morré Department of Biology Valparaiso University Valparaiso USA Mor-NuCo Inc. Purdue Research Park West Lafayette USA

A yeast deletion library was screened based on NADH fluorescence using a 384-well plate assay to identify a yeast isolate lacking a previously identified cell surface oxidase exhibiting an oscillatory pattern with a period length of 25 min and resistant to the ENOX1-specific inhibitor simalikalactone D (YNOX for yeast-specific ENOX = ENOX4). The cDNA was cloned from a yeast over expression library using NADH fluorescence analyzed by Fast Fourier transform and decomposition fits. The objective was to identify and sequence an ENOX homologue in Saccharomyces cerevisiae with a 25 min rather than a 24 min period length (YNOX). The finding identified YDR005C as the yeast ENOX protein with a temperature-independent 25 min period length and insensitive to inhibition by simalikalactone D. The encoded protein was expressed in bacteria and characterized. Gel slices corresponding to 55 kDa and 39 kDa His-tagged proteins exhibited 25 min oscillatory patterns not inhibited by 1 μM simalikalactone D for both NADH oxidation and reduced coenzyme Q10 oxidation as well as a protein disulfide-thiol interchange activity which alternated with the oxidative activities. Activities were phased by low-frequency electromagnetic fields but, in contrast, to yeast ENOX1, not by addition of melatonin. The assay in the presence of D2O shifted the length of the oscillatory period from 25 min to 32 min. The YDR005C deletion mutant cells lacked the ENOX4 clock output present in the wild type yeast.

关键词： Biological Clock ECTO-NOX (ENOX) Proteins YNOX = ENOX4 Ultradian Oscillator Yeast (Saccharomyces cerevisiae)

Induction of Species-Specific Tolerance to Human Islet Xenografts by anti-CD45RB

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Induction of Species-Specific Tolerance to Human Islet Xenog...

Transplantation tolerance that depends on regulatory T cells induced by TGF-beta-producing regulatory B cells

2013中国器官移植大会

作者： Gaoping Zhao Daniel Moore james kim Kang Me Lee Matthew OConnor Maozhu Yang Christian Schuetz Ji Lei james Markmann Shaoping Deng Department of Surgery Sichuan Provincial People's Hospital & Sichuan Academy of Medical Sciences Department of Surgery Massachusetts General HospitalHarvard Medical School Department of Pediatrics and Pathology Microbiologyand ImmunologyIan Burr Division of Endocrinology and DiabetesVanderbilt University

Transplantation for type 1 diabetes is severely limited by the lack of donor islets and by the rapid destruction of transplanted islets by innate and adaptive *** transplantation requires desirable engraftment,avoidance of immediate immune barriers,and long term escape from lymphocyte mediated *** clinical applicability of transplantation could be further enhanced by protocols that permit the use of xenogeneic islets as this advance would markedly increase the availability of donor islets to meet the significant *** addition,xenogeneic tolerance induction would substantially improve the study of human islet biology in animal model *** have utilized treatment with the monoclonal antibody against CD45RB to induce permanent acceptance of human islets in murine ***,depletion of B cells permitted universal tolerance induction andthis tolerance was species—specific.

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Transplantation tolerance that depends on regulatory T cells...

Improvement of Renewable Bioenergy Production in Microbial Fuel Cells with Saponin Supplementation

2013中国器官移植大会

作者： Gaoping Zhao Kang Me Lee Ryan Scott Julie SooHoo Liang Moh Moh Lindsey Fitzgerald Ji Lei Heidi Yeh Shaoping Deng james Markmann james kim Department of Surgery Sichuan Provincial People's Hospital & Sichuan Academy of Medical Sciences Department of Surgery Massachusetts General HospitalHarvard Medical School

Regulatory B cells(Bregs)have been shown to play a critical role in immune homeostasis and in autoimmunity *** have recently demonstrated that combined anti-TIM-1 and anti-CD45RB antibody treatment generates regulatory B cells necessary for long- term mouse islet allograft survival in a full MHC-mismatch donor-recipient pair Lee KM,kim JI,,Stott R,Soohoo J,O’Connor *** H, Zhao G,Eliades P,Fox C,Cheng N,Deng S,Markmann ***-CD45RB/anti-TIM-1-induced tolerance requires regulatory B cells. Am J Transplantation.2012;12:2072—2078).Here we provide evidence that the induction of tolerance by regulatory B cells requires regulatory T ***,adoptive transfer of Bregs results in an increase in absolute number of Tregs in grafted recipients,and depletion of Tregs abrogates transplant tolerance conferred by adoptive transfer of regulatory B *** hypothesize that Bregs induce

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Journal of Sustainable Bioenergy Systems 2021年第2期11卷 82-93页

作者： Skylar Choi Yongjin Park Immanuel H. Anaborne Jin Sik Song Ji Woo Han So Hyun Jeon Jaewoo kim james kim Jinkwon Lee Paul S. Chung Horace Greeley High School Chappaqua NY USA Deerfield Academy Deerfield MA USA Oakton High School Vienna VA USA School of Arts & Sciences University of Virginia Charlottesville VA USA School of Arts & Sciences Emory University Atlanta GA USA Bachelor of Science Villanova University Villanova PA USA College of Letters & Sciences University of California Los Angeles Los Angeles CA USA School of Arts & Sciences University of Michigan Ann Arbor MI USA Division of Biological Sciences Revelle College University of California San Diego CA USA Fuzbien Technology Institute Rockville MD USA

Microbial fuel cell (MFC) is one of renewable biofuel production technology that directly converts biomass to electricity. Cellulosic biomass is particularly attractive renewable resources for its low cost and abundance and neutral carbon balance. However, methanogenesis remains as a major factor limiting MFC performance. The current study reports that saponin addition at 0.05% w/v dose to anolyte in MFCs inhibited methanogenesis and improves power generation and cellulose fermentation. Mediator-less two chamber H-type MFCs were prepared using rumen fluid as anode inocula at 20% v/v of anolyte to convert finely ground pine tree (Avicel) at 2%, w/v to electricity. Saponin was added to the anode of MFC at 0.005% or 0.05% v/v dosage for treatment. MFC power and current across an external resistor were measured daily for 10d. On d10, collected gases from anode compartment were measured for total gas volume and analyzed for gas composition on gas chromatography. Supplementation of saponin to MFC at 0.005% did not have any effects on electricity generation or biogas production and composition. Saponin at 0.05% dose reduced 10% of methane prod

关键词： Microbial Fuel Cells Saponin Bioenergy

Global Genomic Variations of Pathogenic Bacteria <i>Salmonella</i>Reading

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Computational Molecular Bioscience 2021年第3期11卷 57-68页

作者： Woo Hyung Lee Yein Jeon Jonathan S. Chung Woo Jun Shim Min Jae Shin Seok Gyu Han Seunghan Baek james kim Jaewoo kim Paul S. Chung Biotechnology School of Sciences Hong Kong University of Science of Technology Hong Kong China Fuzbien Technology Institute Rockville USA Ernest Mario School of Pharmacy Rutgers University New Brunswick USA College of Letters & Sciences University of California Los Angeles USA Biology Bachelor of Science California Institute of Technology Pasadena USA School of Arts & Sciences Johns Hopkins University Baltimore USA School of Arts & Sciences University of Michigan Ann Arbor USA Youth with Talents Fairfax USA

US Centers for Disease Control and Prevention (CDC) estimates food-borne pathogenic Salmonella bacteria cause about 1.35 million infections, 26,500 hospitalizations, and 420 deaths in the U.S. every year. Outbreaks of multidrug-resistant Salmonella Reading linked mainly to raw turkey products and alfalfa sprouts have accounted for human illness including mortality. Therefore, we downloaded and compared genome sequences of 897 Salmonella Reading isolated from 27 resources in 11 countries at 21 year-points from the National Center for Biotechnology Information database. Isolates were clustered into 10 clades which consisted of 3 major and 7 minor or single node clades. Although 3 major clades included both Europe and American isolates, one clade consisted of 605 North American isolates out of 614 isolates. Evolutionary distance is more related to the continent than the source of isolation. The host source, continent (North America and Europe) and phylogenetic clade were related to the prevalence of isolates encoding Antimicrobial Resistance Genes (ARGs). Prevalence of prophages was greater in bovine and swine isolates than poultry and human isolates and the least prevalence was found in human isolates. Between continents, the prevalence of phage was greater in North Ame- rican isolates than European. The diversity of virulence factors in swine isolates differed from poultry isolate while no difference was found among continents. In conclusion, evolutionary distance is related to isolation host source rather than the continent, and genome features were distinguished by host and cluster. Our genomic analysis implies that Salmonella Reading evolved independently to environments within its lineages.

关键词： Salmonella Serovar Reading Genome Antimicrobial Resistance Genes Virulence Factors Phylogenomic Tree

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价值与成长的演变

金融市场研究 2019年第8期79卷 98-100页

作者：詹姆斯·金刘亚冠(编译) 《财资》杂志不详

价值投资是最久经考验的投资策略,事实证明,从长期来看,在几乎所有资产类别和地区,它都表现强劲。然而,现在我们正处于价值投资史上最糟糕的时期,用一些简单的理由来解释为什么'价值股如今不受欢迎'是不够的。在过去十年里,在几乎所有... 详细信息

价值投资是最久经考验的投资策略,事实证明,从长期来看,在几乎所有资产类别和地区,它都表现强劲。然而,现在我们正处于价值投资史上最糟糕的时期,用一些简单的理由来解释为什么'价值股如今不受欢迎'是不够的。在过去十年里,在几乎所有市场上,成长型股票(不考虑估值,收入和利润都高增长的股票)都超过了价值型股票(较收入、利润和账面价值而言,股票估值低)。

关键词：成长型股票价值投资者价值型股票巴菲特

Analysis of the Oscillatory Flows of Multiple Shock Waves in a Constant Area Duct

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Journal of Thermal Science 2024年第3期33卷 794-806页

作者： K james Jintu kim Heuy Dong Department of Mechanical Engineering Andong National UniversityAndong36729Republic of Korea CAD-IT Korea Co. Ltd.Seoul08591Republic of Korea

The oscillatory response of multiple shock waves to downstream perturbations in a supersonic flow is studied numerically in a rectangular *** shock waves are formed inside the duct at a shock Mach number of *** duct has an exit height of H,and the effect of duct resonance on multiple shock oscillations is investigated by attaching exit ducts of lengths 0H,50H,and *** downstream disturbance frequency varied from 10 Hz to 200 Hz to explore the oscillation characteristics of the multiple shock *** oscillatory response of shock waves under self-excited and forced oscillation conditions are analyzed in terms of wall static pressure,shock train leading-edge location,shock train length,and the size of the separation *** extent of the initial shock location increases with an increase in exit duct length for the self-excited oscillation *** analysis of the shock train leading edge and the spectral analysis of wall static pressure variations are *** variation in the shock train length is analyzed using the pressure ratio method for self-excited as well as forced *** RMS amplitude of the normalized shock train length(ζ_(ST))increases with an increase in the exit duct length for the self-excited oscillation *** the downstream perturbation frequency is increased,ζ_(ST)is decreased for exit duct *** all exit duct designs and downstream forcing frequencies,the size of the separation bubble grows and shrinks during the shock oscillations,demonstrating the dependence on duct resonance and forced oscillations.

关键词： shock train downstream disturbance supersonic flow shock wave boundary layer interaction duct resonance flow separation

Clinical application of liver stiffness measurement using transient elastography in chronic liver disease from longitudinal perspectives

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World Journal of Gastroenterology 2013年第12期19卷 1890-1900页

作者： Beom Kyung kim james Fung Man-Fung Yuen Seung Up kim Department of Internal Medicine Institute of Gastroenterology Yonsei University College of Medicine Liver Cirrhosis Clinical Research Center Department of Medicine State Key Laboratory for Liver Research the University of Hong Kong

Accurate determination of the presence and degree of fibrosis in liver is of great importance, because the prognosis and management strategies for chronic liver disease depend mainly on these factors. To date, liver biopsy (LB) remains the "gold standard" for assessing the severity of liver fibrosis; however, LB is often limited by its invasiveness, sampling error, and intra/ inter-observer variability in histological interpretation. Furthermore, repeated LB examinations within a short time interval are indeed ineligible in a real clinical practice. Thus, due to the pressing need for non-invasive surrogates for liver fibrosis, transient elastography (TE),as a novel ultrasound based technology, has allowed a noninvasive measurement of liver stiffness and has gained in popularity over recent years. In the past few years, additional roles for transient TE beyond the initial purpose of a non-invasive surrogate for LB have included the prediction of the most two critical consequences of fibrosis progression: the development of portal hypertension-related complications and hepatocellular carcinoma. This indicates that the role of transient TE is not merely limited to reducing the need for LB, but transient TE can enable the establishment of tailored management strategies by providing more detailed prognostic information. In particular, under the concept in which the clinical course of liver fibrosis is dynamic and bidirectional, especially when appropriate intervention is commenced, transient TE can be used to track the dynamic changes in fibrotic burden during antiviral or antifibrotic treatment. This review discussed extended applications of transient TE in prediction of the development of real clinical endpoints from a longitudinal perspective.

关键词： Liver stiffness Transient elastography Fibroscan Fibrosis Longitudinal Outcome

MicroRNAs in pancreatic ductal adenocarcinoma

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World Journal of Gastroenterology 2011年第7期17卷 817-827页

作者： Jong Y Park james Helm Domenico Coppola Donghwa kim Mokenge Malafa Seung Joon kim Division of Cancer Prevention and Control Moffitt Cancer Center FL 33612 United States Gastrointestinal Tumor Program Moffitt Cancer Center FL 33612 United States Department of Anatomic Pathology Moffitt Cancer Center FL 33612 United States Department of Molecular Oncology MoffittCancer Center FL 33612 United States Department of Internal Medicine Collegeof Medicine the Catholic University of Korea Seoul 137-040South Korea

Ductal adenocarcinoma of the pancreas is a lethal cancer for which the only chance of long-term survival belongs to the patient with localized disease in whom a potentially curative resection can be done. Therefore, biomarkers for early detection and new therapeutic strategies are urgently needed. miRNAs are a recently discovered class of small endogenous non-coding RNAs of about 22 nucleotides that have gained attention for their role in downregulation of mRNA expression at the post-transcriptional level. miRNAs regulate proteins involved in critical cellular processes such as differentiation, proliferation, and apoptosis. Evidence suggests that deregulated miRNA expression is involved in carcinogenesis at many sites, including the pancreas. Aberrant expression of miRNAs may upregulate the expression of oncogenes or downregulate the expression of tumor suppressor genes, as well as play a role in other mechanisms of carcinogenesis. The purpose of this review is to summarize our knowledge of deregulated miRNA expression in pancreatic cancer and discuss the implication for potential translation of this knowledge into clinical practice.

关键词： MicroRNAs Pancreatic cancer