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Effect of Probiotics on Juvenile Tachypleus tridentatus Gut Microbiota

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Journal of Ocean University of China 2022年第3期21卷 564-572页

作者： XIONG kai MIAO Fengze WAIHO khor LIU Ximei FANG james k.H. HE Jinfeng HU Menghong WANG Youji key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai201306China Higher Institution Center of Excellence(HICoE) Institute of Tropical Aquaculture and FisheriesUniversity Malaysia TerengganuKuala TerengganuTerengganu20000Malaysia Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloon999077China Beihai Product Quality Testing Institute Beihai536000China

The population of tri-spine horseshoe crabs(HSCs)Tachypleus tridentatus has sharply decreased in recent *** and manual releasing are now used to recover *** order to accelerate the growth of *** in indoor environment,in this study,gut microbiota of 2nd instar *** was supposed to be improved by putting probiotics in water environment and thus promoted the health and growth of the *** 16S rRNA sequencing of gut samples,we examined the intestinal microbiome of juvenile HSCs and studied the effect of *** showed that there was no significant difference between groups fed with high and low concentrations(1×10^(7),1×10^(12)CFU mL^(−1))of Bacillus subtilis and high concentration(1×10^(12)CFU mL^(−1))of Lactobacillus,and no significant difference compared with the control group,but the diversity of Lactobacillus group with low concentration(1×10^(7)CFU mL^(−1))was significantly higher than those of other ***-mediated functions predicted by PICRUSt showed that‘Cellular Processes’,‘Metabolism’and‘Organismal Systems’pathways significantly increased in groups of Bacillus subtilis and Lactobacillus group with low *** results showed that two types of probiotics with different concentrations have other effects on the structure,richness and function of intestinal *** searching the literature and conducting pre-experiments,we forecasted the short time of breeding under the probiotic environment may not have much *** present study for the first time investigated the impact of probiotics on gut microbiota of juvenile HSCs and found them may benefit for the growth and health of HSCs.

关键词： Tachypleus tridentatus probiotic gut microbiota diversity richness

A classical equation that accounts for observations of non-Arrhenius and cryogenic grain boundary migration

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npj Computational Materials 2022年第1期8卷 1492-1500页

作者： Eric R.Homer Oliver k.Johnson Darcey Britton james E.Patterson Eric T.Sevy Gregory B.Thompson Department of Mechanical Engineering Brigham Young UniversityProvoUT84602USA Department of Chemistry and Biochemistry Brigham Young UniversityProvoUT84602USA Department of Metallurgical and Materials Engineering University of AlabamaTuscaloosaAL35401USA

Observations of microstructural coarsening at cryogenic temperatures,as well as numerous simulations of grain boundary motion that show faster migration at low temperature than at high temperature,have been troubling because they do not follow the expected Arrhenius *** work demonstrates that classical equations,that are not simplified,account for all these oddities and demonstrate that non-Arrhenius behavior can emerge from thermally activated *** to this classical model,this occurs when the intrinsic barrier energies of the processes become small,allowing activation at cryogenic *** thermal energy then allows the low energy process to proceed in reverse,so increasing temperature only serves to frustrate the forward *** classical form is shown to reconcile and describe a variety of diverse grain boundary migration observations.

关键词： cryogenic grain boundary

Deposition of nanoparticles on school eyeglasses in urban and rural areas:A methodology for a more real assessment of the possible impacts

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Geoscience Frontiers 2022年第1期13卷 498-505页

作者： kátia Martinello james C.Hower Guilherme L.Dotto Claudete G.Ramos Carlos E.Schnorr Diana Pinto Instituto Inanís Rua Felizardo FurtadoPorto AlegreBrazil University of kentucky Department of Earth&Environmental SciencesLexingtonKY 40506USA University of kentucky Center for Applied Energy Research2540 Research Park DriveLexingtonKY 40511USA Chemical Engineering Department Federal University of Santa Maria97105-900 Santa MariaBrazil Department of Civil and Environmental Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia

Because incomplete confirmation is available concerning the influential role of atmosphere contamination on conjunctivitis,myopia,asthma,and allergic rhinitis in Brazil,the focus of the present work is to explore the possible relations among atmosphere contamination and eye *** that a case study on eye diseases,by way of questionnaires supplemented by the investigation of nanoparticles(NPs)on eyeglasses,the study examines the mechanisms in which NPs and ultra-fine particles are deposited on the glasses of children up to 10 years of age in urban and rural *** important connection between atmosphere contaminants and individual protection equipment justifies improving indoor school properties in order could protect children’s eyes,particularly in high-pollution/high-particulate areas.

关键词： Indoor air pollution Nanoparticles Eyes health Allergic reaction Schools

Controlled Vertical Transfer of Individual Au Atoms Using a Surface Supported Carbon Radical for Atomically Precise Manufacturing

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Precision Chemistry 2023年第2期1卷 119-126页

作者： Pallavi Bothra Adam Z.Stieg james k.Gimzewski Philippe Sautet Chemical and Biomolecular Engineering Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States California NanoSystems Institute University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States Chemistry and Biochemistry Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States Chemical and Biomolecular Engineering Department and Chemistry and Biochemistry Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States

To explore a proof-of-concept for atomically precise manufacturing(APM)using scanning probe microscopy(SPM),first principle theoretical calculations of atom-by-atom transfer from the apex of an SPM tip to an individual radical on a surfacebound organic molecule have been *** transfer is achieved by spatially controlled motion of a gold terminated tip to the *** molecular tools for SPM-based APM have been designed and investigated,each comprising an adamantane core,a radical end group,and trithiol linkers to enable strong chemisorption on the Au(111)surface:ethynyl-adamantanetrithiol and *** demonstrate the details of controlled Au atom abstraction during tip approach toward and retraction from the radical *** approach of the tip,the apical Au atom undergoes a transfer toward the carbon radical at a clearly defined threshold *** atomic displacement is accompanied by a net energy gain of the system in the range−0.5 to−1.5 eV,depending on the radical *** the case of a triangular pyramidal apex model,two tip configurations are possible after the tip atom displacement:(1)an Au atom is abstracted from the tip and bound to the C radical,not bound to the tip base anymore,and(2)apical tip atoms rearrange to form a continuous neck between the tip and *** the second case,subsequent tip retraction leads to the same final configuration as the first,with the abstracted Au atom bound to radical carbon atom of the molecular *** the less reactive adamantyl-trithiol radical molecular tool,Au atom transfer is less energetically favored,but this has the advantage of avoiding other apex gold atoms from rearrangement.

关键词： atomically precise manufacturing scanning probe microscopy density functional theory atom vertical transfer molecular radical

CD8+T cell metabolic flexibility elicited by CD28-ARS2 axisdriven alternative splicing of PkM supports antitumor immunity

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Cellular & Molecular Immunology 2024年第3期21卷 260-274页

作者： G.Aaron Holling Colin A.Chavel Anand P.Sharda Mackenzie M.Lieberman Caitlin M.james Shivana M.Lightman Jason H.Tong Guanxi Qiao Tiffany R.Emmons Thejaswini Giridharan Shengqi Hou Andrew M.Intlekofer Richard M.Higashi Teresa W.M.Fan Andrew N.Lane kevin H.Eng Brahm H.Segal Elizabeth A.Repasky kelvin P.Lee Scott H.Olejniczak Department of Immunology Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Department of Medicine Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Human Oncology and Pathogenesis Program Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Center for Environmental Systems Biochemistry Department of Toxicology and Cancer Biology and Markey Cancer CenterLexingtonKY 40536USA Department of Cancer Genetics and Genomics Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Present address:University of Colorado Boulder BoulderCO 80309USA Present address:Dana Farber Cancer Institute BostonMA 02215USA Present address:Massachusetts Institute of Technology BostonMA 02139USA Present address:Melvin and Bren Simon Comprehensive Cancer Center Indiana University School of MedicineIndianapolisIN 46202USA

Metabolic flexibility has emerged as a critical determinant of CD8+T-cell antitumor activity,yet the mechanisms driving the metabolic flexibility of T cells have not been *** this study,we investigated the influence of the nuclear cap-binding complex(CBC)adaptor protein ARS2 on mature T *** doing so,we discovered a novel signaling axis that endows activated CD8+T cells with flexibility of glucose ***2 upregulation driven by CD28 signaling reinforced splicing factor recruitment to pre-mRNAs and affected approximately one-third of T-cell activation-induced alternative splicing *** these effects,the CD28-ARS2 axis suppressed the expression of the M1 isoform of pyruvate kinase in favor of PkM2,a key determinant of CD8+T-cell glucose utilization,interferon gamma production,and antitumor effector ***,PkM alternative splicing occurred independently of CD28-driven PI3k pathway activation,revealing a novel means by which costimulation reprograms glucose metabolism in CD8+T cells.

关键词： Immunometabolism mRNA splicing CD8 T cells ARS2 PkM2

A“Sequential Design of Simulations”approach for exploiting and calibrating discrete element simulations of cohesive powders

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Frontiers of Chemical Science and Engineering 2022年第6期16卷 874-885页

作者： Xizhong Chen Chunlei Pei james A.Elliott Department of Materials Science and Metallurgy University of CambridgeCambridge CB3 OFSUK Process and Chemical Engineering School of EngineeringUniversity College CorkCork T12 K8AFIreland Department of Chemical and Biological Engineering The University of SheffieldSheffield S102TNUK key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

The flow behaviours of cohesive particles in the ring shear test were simulated and examined using discrete element method guided by a design of experiments methodology.A full factorial design was used as a screening design to reveal the effects of material properties of *** augmented design extending the screening design to a response surface design was constructed to establish the relations between macroscopic shear stresses and particle *** is found that the powder flow in the shear cell can be classified into four *** stress is found to be sensitive to particle friction coefficient,surface energy and Young’s modulus.A considerable fluctuation of shear stress is observed in high friction and low cohesion *** high cohesion regime,Young’s modulus appears to have a more significant effect on the shear stress at the point of incipient flow than the shear stress during the pre-shear *** predictions from response surface designs were validated and compared with shear stresses measured from the Schulze ring shear *** is found that simulations and experiments showed excellent agreement under a variety of consolidation conditions,which verifies the advantages and feasibility of using the proposed“Sequential Design of Simulations”approach.

关键词： discrete element method cohesive materials parameter calibration ring shear cell design of experiments

Integrating Early Cretaceous fossils into the phylogeny of living angiosperms:Magnoliidae and eudicots

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Journal of Systematics and Evolution 2010年第1期48卷 1-35页

作者： james A.DOYLE Peter k.ENDRESS Department of Evolution and Ecology University of California DavisCA 95616 USA Institute of Systematic Botany University of Zurich 8008 ZurichSwitzerland

Over the past 25 years, discoveries of Early Cretaceous fossil flowers, often associated with pollen and sometimes with vegetative parts, have revolutionized our understanding of the morphology and diversity of early ***, few of these fossils have been integrated into the increasingly robust phylogeny of living angiosperms based primarily on molecular *** remedy this situation, we have used a morphological dataset for living basal angiosperms(including basal eudicots and monocots) to assess the most parsimonious positions of early angiosperm fossils on cladograms of Recent plants, using constraint trees that represent the current range of hypotheses on higher-level relationships, and concentrating on Magnoliidae(the clade including Magnoliales, Laurales, Canellales, and Piperales) and *** magnoliids, our results confirm proposed relationships of Archaeanthus(latest Albian?) to Magnoliaceae, Endressinia(late Aptian) to Magnoliales(the clade comprising Degeneria, Galbulimima, Eupomatia, and Annonaceae), and Walkeripollis pollen tetrads(late Barremian?) to Winteraceae, but they indicate that Mauldinia(early Cenomanian) was sister to both Lauraceae and Hernandiaceae rather than to Lauraceae *** middle Albian to early Cenomanian eudicots, we confirm relationships of Nelumbites to Nelumbo, platanoid inflorescences and Sapindopsis to Platanaceae, and Spanomera to *** the possible exception of Archaeanthus, these fossils are apparently not crown group members of living families but rather stem relatives of one or more families.

关键词： angiosperms Cretaceous eudicots flowers Magnoliidae paleobotany phylogeny pollen.

Artisanal ceramic factories using wood combustion:A nanoparticles and human health study

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Geoscience Frontiers 2022年第1期13卷 488-497页

作者： kátia Martinello james C.Hower Diana Pinto Carlos E.Schnorr Guilherme L.Dotto Marcos Leandro Silva Oliveira Claudete G.Ramos Instituto Inanís Rua Felizardo FurtadoPorto AlegreBrazil Department of Civil and Environmental Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia University of kentucky Department of Earth&Environmental SciencesLexingtonKY 40506USA University of kentucky Center for Applied Energy Research2540 Research Park DriveLexingtonKY 40511USA Centro de Estudos em Estresse Oxidativo-Departamento de Bioquímica-Instituto de Ciências Básicas da Saúde-Universidade Federal do Rio Grande do Sul(UFRGS) Porto AlegreRSBrazil Chemical Engineering Department Federal University of Santa MariaUFSMRoraima Avenue1000Santa MariaRS97105-900Brazil Departamento de Ingeniería Civil y Arquitectura Universidad de LimaAvenida Javier Prado Este 4600–Santiago de Surco 1503Peru

The ceramics industry,resulting from developments of modern compounds,is a segment of great influence in worldwide *** ceramic factories based on wood combustion have significant risks for the creation and discharge of atmosphere nanoparticles(NPs)and ultra-fine particles(UFPs).At present,there is insufficient recognition on the influence of engineered-NPs on the atmosphere and *** improvements are indispensable to diminish contact with *** present study demonstrates the main NPs and UFPS present in an area of intense artisanal wood-combustion ceramic *** matter was sampled for morphological,chemical,and geochemical studies by sophisticated electron microbeam microscopy,X-Ray Diffraction,and Raman *** NPs configuration(10 nm)were produced around the studied artisanal ceramic *** study presents an indication of the recent information on population and work-related contact to NPs in the artisanal ceramic factories and their influence on health.

关键词： Ceramic manufacturing Nanoparticles Advanced characterization Multiple impacts

Meteorite impact craters as hotspots for mineral resources and energy fuels:A global review

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Energy Geoscience 2022年第2期3卷 136-146页

作者： S.james Saranya R.Chandran M.Santosh A.P.Pradeepkumar M.N.Praveen k.S Sajinkumar s sajinkumar 1 2department of geologyuniversity of keralathiruvananthapuram695581keralaindia school of earth science and resources china university of geosciencesbeijing100083china department of earth sciences university of adelaidesa5005australia geological survey of india bengaluru560111india department of geological and mining engineering and sciences michigan technological universityhoughton49931miusa

The ever-increasing recovery rate of natural resources from terrestrial impact craters over the last fewdecades across the globe offers new avenues for further exploration of mineral and hydrocarbon resources in such *** of today,60 of the 208 terrestrial craters have been identified to host diverseresources such as hydrocarbons,metals and construction *** potential of craters as plausibleresource contributors to the energy sector is therefore,worthy of consideration,as 42(70%)of the 60craters host energy resources such as oil,gas,coal,uranium,mercury,critical and major minerals as wellas hydropower *** others,19 craters are of well-developed hydrocarbon *** associated with craters are also classified similar to other mineral resources such as progenetic,syngenetic and epigenetic *** these,the progenetic and syngenetic mineralization are confinedto the early and late excavation stage of impact crater evolution,respectively,whereas epigenetic deposits are formed during and after the modification stage of crater ***,progenetic andsyngenetic mineral deposits(like Fe,Ni,Pb,Zn and Cu)associated with craters are formed as a directresult of the impact event,whereas epigenetic deposits(***)are hosted by the impactstructure and result from post-impact *** the progenetic and syngenetic deposits,the shockwave induced fracturing and melting aid the formation of deposits,whereas in the epigenetic deposits,the highly fractured lithostratigraphic units of higher porosity and permeability,like the centralelevated area(CEA)or the rim,act as *** this review,we provide a holistic view of the mineral andenergy resources associated with impact craters,and use some of the remote sensing techniques toidentify the mineral deposits as supplemented by a schematic model of the types of deposits formedduring cratering process.

关键词： Meteorite impact crater Progenetic deposit Syngenetic deposit Epigenetic deposit Mineralization Hydrocarbon Petroleum Exploration