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Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and k.aya senegalensis

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Journal of Integrative Medicine 2017年第3期15卷 214-230页

作者： Jeremiah Olorunjuwon Olugbami Robert Damoiseaux Bryan France Michael A. Gbadegesin Adam Z. Stieg Shivani Sharma Oyeronk. A. Odunola james k. gimzewski Cancer Research and Molecular Biology Laboratories Department of Biochemistry College of Medicine University of Ibadan Ibadan Oyo 200005 Nigeria Nano and Pico Characterization Laboratories California NanoSystems Institute University of California LosAngeles California 90095 USA Department of Chemistry and Biochemistry University of California Los Angeles California 90095 USA Molecular Screening and Shared Resources Laboratories California NanoSystems Institute University of California Los Angeles California 90095 USA Department of Molecular and Medicinal Pharmacology University of California Los Angeles California 90095 USA International Center for Materials Nanoarchitectonics Satellite National Institute for Materials Science Tsukuba 3690114 Japan

OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of k.aya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract. METHODS： Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows： nanotechnologically--atomic force microscopy （AFM） was used to determine cell stiffness; biochemically--cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically--a carrot disk.assay was used to assess the anticancer property of the extract of k. senegalensis. RESULTS： Curcumin and k. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox （glutathione level） and energy （adenosine triphosphate） status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlik. the k. senegalensis extract. In addition the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk.assay. CONCLUSION： Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of k. senegalensis exhibited antimetastatic properties on HepG2 cells.

关键词： Curcumin k.aya senegalensis atomic force microscopy glutathione adenosine triphosphate metasis carcinoma, hepatocellular

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Controlled Vertical Transfer of Individual Au Atoms Using a Surface Supported Carbon Radical for Atomically Precise Manufacturing

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Precision Chemistry 2023年第2期1卷 119-126页

作者： Pallavi Bothra Adam Z.Stieg james k.gimzewski Philippe Sautet Chemical and Biomolecular Engineering Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States California NanoSystems Institute University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States Chemistry and Biochemistry Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States Chemical and Biomolecular Engineering Department and Chemistry and Biochemistry Department University of CaliforniaLos AngelesLos AngelesCalifornia 90095United States

To explore a proof-of-concept for atomically precise manufacturing(APM)using scanning probe microscopy(SPM),first principle theoretical calculations of atom-by-atom transfer from the apex of an SPM tip to an individual radical on a surfacebound organic molecule have been *** transfer is achieved by spatially controlled motion of a gold terminated tip to the *** molecular tools for SPM-based APM have been designed and investigated,each comprising an adamantane core,a radical end group,and trithiol link.rs to enable strong chemisorption on the Au(111)surface:ethynyl-adamantanetrithiol and *** demonstrate the details of controlled Au atom abstraction during tip approach toward and retraction from the radical *** approach of the tip,the apical Au atom undergoes a transfer toward the carbon radical at a clearly defined threshold *** atomic displacement is accompanied by a net energy gain of the system in the range−0.5 to−1.5 eV,depending on the radical *** the case of a triangular pyramidal apex model,two tip configurations are possible after the tip atom displacement:(1)an Au atom is abstracted from the tip and bound to the C radical,not bound to the tip base anymore,and(2)apical tip atoms rearrange to form a continuous neck.between the tip and *** the second case,subsequent tip retraction leads to the same final configuration as the first,with the abstracted Au atom bound to radical carbon atom of the molecular *** the less reactive adamantyl-trithiol radical molecular tool,Au atom transfer is less energetically favored,but this has the advantage of avoiding other apex gold atoms from rearrangement.

关键词： atomically precise manufacturing scanning probe microscopy density functional theory atom vertical transfer molecular radical

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