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Absence of Na^+/sugar cotransport activity in Barrett's metaplasia

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World journal of Gastroenterology 2008年第9期14卷 1365-1369页

作者： Lisa j Murray Owen Tully David S Rudolph Marysue Whitby Mary C Valenzano Giancarlo Mercogliano james j Thornton james M Mullin Lankenau Institute for Medical Research WynnewoodPAUnited States Saint Joseph's UniversityDepartment of BiologyPhiladelphiaPA 19096United States Lankenau Hospital Department of Medicine WynnewoodPA 19096United States Lankenau Hospital Department of MedicineWynnewoodPA 19096 United States Lankenau Institute for Medical Research WynnewoodPAUnited States Lankeanu Hospital Division of GastroenterologyWynnewood PA 19096United States Lankeanu Hospital Division of GastroenterologyWynnewoodPA 19096United States

AIM:To evaluate the presence of Na+-dependent, active, sugar transport in Barrett's epithelia as an intestinal biomarker, based on the well-documented, morphological intestinal phenotype of Barrett's esophagus (BE). METHODS: We examined uptake of the nonmeta- bolizable glucose analogue, alpha-methyl-D-glucoside (AMG), a substrate for the entire sodium glucose cotransporter (SGLT) family of transport proteins. During upper endoscopy, patients with BE or with uncomplicated gastroesophageal reflux disease (GERD) allowed for duodenal, gastric fundic, and esophageal mucosal biopsies to be taken. Biopsies were incubated in bicarbonate-buffered saline (KRB) containing 0.1 mmol/L 14C-AMG for 60 min at 20℃. Characterized by abundant SGLT, duodenum served as a positive control while gastric fundus and normal esophagus, known to lack SGLT, served as negative controls. RESULTS: Duodenal biopsies accumulated 249.84 ± 35.49 (SEM) picomoles AMG/μg DNA (n = 12), gastric fundus biopsies 36.20 ± 6.62 (n = 12), normal esophagus 12.10 ± 0.59 (n = 3) and Barrett's metaplasia 29.79 ± 5.77 (n = 8). There was a statistical difference (P < 0.01) between biopsies from duodenum and each other biopsy site but there was no statistically significant difference between normal esophagus and BE biopsies. 0.5 mmol/L phlorizin (PZ) inhibited AMG uptake into duodenal mucosa by over 89%, but had nosignificant effect on AMG uptake into gastric fundus, normal esophagus, or Barrett's tissue. In the absence of Na+ (all Na+ salts replaced by Li+ salts), AMG uptake in duodenum was decreased by over 90%, while uptake into gastric, esophageal or Barrett's tissue was statistically unaffected. CONCLUSION: Despite the intestinal enterocyte phenotype of BE, Na+-dependent, sugar transport activity is not present in these cells.

关键词： Barrett's esophagus Cancer Biomarker Sodium glucose cotransporters Glucose transport Alphamethyl-D-glucoside Phlorizin Esophagus Metaplasia

Adenosine-treated bioprinted muscle constructs prolong cell survival and improve tissue formation

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Bio-Design and Manufacturing 2021年第3期4卷 441-451页

作者： Dongxu Ke Adam M.jorgensen Sang j.Lee james j.Yoo Sean V.Murphy Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNCUSA Novaprint Therapeutics Suzhou Co. LtdSuzhouJiangsuChina

It is crucial to maintaining the viability of biofabricated human-sized tissues to ensure their successful survival and function after *** is a purine nucleoside that has the function to suppress cellular metabolism and has been previously proposed as a method to prolong cell viability under *** this study,we optimized the dose concentration of adenosine for incorporation into bioprinted constructs to preserve long-term cell viability in *** results showed that muscle cells(C2C12)containing 6,7,or 8 mM adenosine maintained high cell viability for 20 days under hypoxic conditions(0.1%O2),whereas cells without adenosine treatment showed 100%cell death after 11 *** 20 days under hypoxic conditions,muscle cells treated with adenosine proliferated and differentiated when transferred to normoxic *** these adenosine concentrations,6 mM was picked as the optimized adenosine concentration for further investigations due to its most effective results on improving cell *** bioprinted muscle constructs containing adenosine(6 mM)maintained high cell viability for 11 days under hypoxic conditions,while the control constructs without adenosine had no live *** in vivo validation,the bioprinted constructs with adenosine implanted under the dorsal subcutaneous space in mice,showed the enhanced formation of muscle tissue with minimal central necrosis and apoptosis,when compared to the constructs without *** positive in vitro and in vivo results demonstrate that the use of adenosine is an effective approach to preventing the challenge of hypoxia-induced necrosis in bioprinted tissues for clinical translation.

关键词： Bioprinting Hypoxia Adenosine Cell viability In vivo

Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells

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journal of Animal Science and Biotechnology 2015年第2期6卷 230-251页

作者： Nuradilla Mohamad-Fauzi Pablo j Ross Elizabeth A Maga james D Murray Department of Animal Science University of California Institute of Ocean and Earth Sciences University of Malaya Department of Population Health and Reproduction University of California

Background： There is considerable interest in using goats as models for genetically engineering dairy animals and also for using stem cells as therapeutics for bone and cartilage repair. Mesenchymal stem cells （MSCs） have been isolated and characterized from various species, but are poorly characterized in goats. Results： Goat MSCs isolated from bone marrow （BM-MSCs） and adipose tissue （ASCs） have the ability to undergo osteogenic, adipogenic and chondrogenic differentiation. Cytochemical staining and gene expression analysis show that ASCs have a greater capacity for adipogenic differentiation compared to BM-MSCs and fibroblasts. Different methods of inducing adipogenesis also affect the extent and profile of adipogenic differentiation in MSCs. Goat fibroblasts were not capable of osteogenesis, hence distinguishing them from the MSCs. Goat MSCs and fibroblasts express CD90, CD105, CD73 but not CD45, and exhibit cytoplasmic localization of OCT4 protein. Goat MSCs can be stably transfected by Nucleofection, but, as evidenced by colony-forming efficiency （CFE）, yield significantly different levels of progenitor cells that are robust enough to proliferate into colonies of integrants following G418 selection. BM-MSCs expanded over increasing passages in vitro maintained karyotypic stability up to 20 passages in culture, exhibited an increase in adipogenic differentiation and CFE, but showed altered morphology and amenability to genetic modification by selection. Conclusions： Our findings provide characterization information on goat MSCs, and show that there can be significant differences between MSCs isolated from different tissues and from within the same tissue. Fibroblasts do not exhibit trilineage differentiation potential at the same capacity as MSCs, making it a more reliable method for distinguishing MSCs from fibroblasts, compared to cell surface marker expression.

关键词： Adipose Bone marrow Characterization Differentiation Goat Mesenchymal stem cells

A linear regression model (LRM) for groundwater chemistry in and around the Vaniyambadi industrial area, Tamil Nadu, India

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Chinese journal Of Geochemistry 2013年第1期32卷 19-26页

作者： Sajil Kumar P.j. Davis Delson P. Vernon j.G. james E.j. Brandenburg Technical University Cottbus-03046 Germany ITALCONSULT Riyadh-11421 Saudi Arabia Budapest University of Technology and Economics (BME) Budapest-Pf. 91 H-152 Hungary Water Institute Karunya UniversityCoimbatore-641114 India

A linear regression model in conjunction with cluster analysis was applied to the groundwater quality parameters for the Vaniyambadi industrial area, Tamil Nadu, India. These physico-chemical parameters were collected from 25 wells by intensive groundwater sampling conducted during january 2010. All the major ions, pH and electrical conductivity were analyzed. The abundances of cations were in the order of Na jor clusters representing the influence of tanneries and geological formations in the study area, which confirmed the results of major ion chemistry.

关键词：线性回归模型地下水化学工业区印度 LRM 聚类分析物理化学参数研究区域

Overview and comparison of guidelines for management of pancreatic cystic neoplasms

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World journal of Gastroenterology 2019年第31期25卷 4405-4413页

作者： Aws Hasan Kavel Visrodia james j Farrell Tamas A Gonda Division of Digestive and Liver Diseases Department of MedicineColumbia UniversityNew YorkNY 10032United States Division of Digestive Diseases Department of MedicineYale UniversityNew HavenCT 06520United States

Pancreatic cysts are identified at an increasing *** mucinous cystic neoplasms represent a pre-malignant condition,the majority of these lesions do not progress to *** the last 10 years several societies have established guidelines for the diagnosis,initial evaluation and surveillance of these *** we provide an overview of five commonly used guidelines:2015 American Gastroenterological Association,2017 International Association of Pancreatology,American College of Gastroenterology 2018,European Study Group and American College of *** describe the similarities and differences between the methods used to formulate these guidelines,the population they target and their approaches towards initial evaluation and surveillance of cystic lesions.

关键词： Pancreatic cyst surveillance Cyst malignancy Guidelines

Potential Applications of Polymer Microchips in Pharmaceuticals

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材料科学与工程学报 2000年第Z2期20卷 993-1000页

作者： james j. Bao Prcoter & Gamble Pharmaceuticals P.O.Box 8006 MasonOH 45040USA

Some of the most recent development in polymer based microchips have been reviewed. Different methods used in fabricating ploymer microchips are introduced. The inportance and applications of the ploymer microchip in pharmaceutical and other areas are discussed. Finally, the potential problems and development areas in polymer microchip technology are also addressed.

关键词： used in

Simulation study of huff-n-puff air injection for enhanced oil recovery in shale oil reservoirs

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Petroleum 2018年第1期4卷 7-14页

作者： Hu jia james j.Sheng State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduSichuan 610500China Bob L.Herd Department of Petroleum Engineering Texas Tech UniversityLubbockTX 79409USA

This paper is the first attempt to evaluate huff-n-puff air injection in a shale oil reservoir using a simulation *** mechanisms and physical processes of huff-n-puff air injection in a shale oil reservoir are investigated through investigating production performance,thermal behavior,reservoir pressure and fluid saturation *** flooding is used as the basic case for a comparative *** simulation study suggests that thermal drive is the main recovery mechanism for huff-n-puff air injection in the shale oil reservoir,but not for simple air *** synergic recovery mechanism of air flooding in conventional light oil reservoirs can be replicated in shale oil reservoirs by using air huff-npuff injection *** huff-n-puff time is better for performing the synergic recovery mechanism of air injection.O2 diffusion plays an important role in huff-n-puff air injection in shale oil *** transmissibility as well as reservoir pressure maintenance ability in huff-n-puff air injection is more pronounced than the simple air flooding after primary depletion *** obvious gas override is exhibited in both air flooding and air huff-n-puff injection scenarios in shale ***-puff air injection has great potential to develop shale oil *** results from this work may stimulate further investigations.

关键词： Air injection huff-n-puff Shale reservoirs Unconventional Air flooding Enhanced oil recovery

China's Investments in Skills

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Frontiers of Economics in China-Selected Publications from Chinese Universities 2018年第4期13卷 531-558页

作者： james j.Heckman Shuaizhang Feng Center for the Economics of Human Development University of ChicagoChicagoIL 60637USA Institute for Economic and Social Research Jinan UniversityGuangzhou 510632China

This paper discusses the benefits of investment in skills in China. We highlight the achievements China has made over time in human capital investments and the new challenges that have emerged as the country develops. To fuel China’s further economic growth and social developments, it is essential to take a more holistic view on skill investments. We suggest policies that promote both economic efficiency and social mobility.

关键词： human capital vocational training migration China

Discussion of the feasibility of air injection for enhanced oil recovery in shale oil reservoirs

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Petroleum 2017年第2期3卷 249-257页

作者： Hu jia james j.Sheng State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduSichuan610500China Bob L.Herd Department of Petroleum Engineering Texas Tech UniversityLubbockTX 79409USA

Air injection in light oil reservoirs has received considerable attention as an effective,improved oil recovery process,based primarily on the success of several projects within the Williston Basin in the United *** main mechanism of air injection is the oxidation behavior between oxygen and crude oil in the *** injection is a good option because of its wide availability and low *** air injection can be applied to shale is an interesting topic from both economic and technical *** paper initiates a comprehensive discussion on the feasibility and potential of air injection in shale oil reservoirs based on state-of-the-art literature *** and unfavorable effects of using air injection are discussed in an analogy analysis on geology,reservoir features,temperature,pressure,and petrophysical,mineral and crude oil properties of shale oil *** available data comparison of the historically successful air injection projects with typical shale oil reservoirs in the *** summarized in this *** operation methods to improve air injection performance are *** paper provides an avenue for us to make use of many of the favorable conditions of shale oil reservoirs for implementing air injection,or air huff‘n’puff injection,and the low cost of air has the potential to improve oil recovery in shale oil *** analysis may stimulate further investigation.

关键词： shale reservoirs reservoir