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Asian Pacific Journal of Tropical Medicine 2012年第9期5卷 718-721页

作者： Varsha Gupta Shailpreet Sidhu jagdish chander Department of Microbiology CovtMedical College & HospitalSector-32ChandigarhIndia

Objective:To detect and evaluate the various methods for metallo-β-lactamases(MBL) production in Pseudomonas aeruginosa(***) and Acinetobacter ***:A total of 109 *** and 85 Acinetobacter species were screened for imipenem resistance by Kirby- Bauer disc diffusion *** of MBL production was(lone by imipenem-EDTA combined disc test,double disc synerygy test(DDST) and imipenem-EDTA MBL E ***: A total of 63(57.8%) strains of *** and 46(54.1%) strains of Acinetobacter *** found to be resistant to *** the 63 imipenem resistant *** tested for MBL production.44(69.89;) were found to be positive and among 46 imipenem resistant Acinetobacter. 19(41.3%) were shown to be the MBL ***:Imipenem-EDTA combined disc test and MBL E test are equally effective for MBL detection in both *** and Acinetobacter spp.,but given the cost-constraints,combined disc can be used as a convenient screening method in the clinical microbiology laboratory.

关键词： Carbapenem-imipenem Metallo-β-lactamases Non fermentative gram-negative bacilli

Evaluation of NS1Ag and IgM antibodies against dengue,importance for epidemiological surveillance

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Asian Pacific Journal of Tropical Medicine 2013年第3期6卷 251-252页

作者： Nidhi Singla Prabhjot Kaur jagdish chander Department of Microbiology Government Medical College HospitalChandigarhIndia

As per the World Health Organization(WHO),a total of 100 tropical and subtropical countries are endemic for dengue, India being one of them[1].Due to the severe complications involved,non-availability of an effective preventive measure in the form of vaccination and the patient management relying largely on good supportive care,there are enough reasons explaining the importance of early

关键词： IgM Ag

Outbreak of Salmonella Typhi enteric fever in sub-urban area of North India:A public health perspective

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Asian Pacific Journal of Tropical Medicine 2013年第2期6卷 167-168页

作者： Nidhi Singla Neha Bansal Varsha Gupta jagdish chander Department of Microbiology Government Medical College HospitalChandigarh160030India

Outbreaks of enteric fever are a major health concern not only due to significant human morbidity and mortality but also fear of spread of multidrug resistant *** report an outbreak of enteric fever caused by Salmonella enterica serotype Typhi in a suburban area,in city Chandigarh of North ***-seven strains of *** were isolated from blood cultures over a period of two weeks with 18 of these 27 patients residing in the same *** cases were in the age group 5-14 years(10 patients,55.5%) while 4(22.2%) cases were children under 5 *** the strains showed similar resistogram being resistant to ampicillin and nalidixic acid,intermediate to ciprofloxacin and sensitive to chloramphenicol,ceftriaxone,cefotaxime,cotrimoxazole and azithromycin on disc diffusion *** inhibitory concentration of ciprofloxacin was determined by agar dilution method and was found to be raised(≥2μg/mL).This nalidixic acid resistant *** outbreak report warrants the necessity of implementing stringent sanitation practices in public health interest.

关键词： Enteric fever Fluoroquinolones Outbreak Salmonella

Salmonella-Salmonellosis-Rare presentations of a common pathogen

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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 417-420页

作者： Neelam Kaistha Varsha Gupta Shailpreet Sidhu jagdish chander Department of Microbiology Government Medical College & Hospital-32ChandigarhIndia

Salmonella are most commonly associated with gastroenteritis and enteric fever in humans. Occasionally,dissemination of bacilli throughout the body results in establishment of localized foci of persistent infection especially in patients with debilitating diseases and immunosuppressive *** at various aberrant sites due to Salmonella has been reported relatively *** has perfected the art of intracellular survival in niches from where they can cause myriad of *** cases with Salmonella infection at unusual sites without any preexisting or underlying disease diagnosed over a period of two years are presented *** etiology was not suspected in these patients and the diagnosis was made microbiologically only after culture isolation.

关键词： Salmonella Aberrant sites Localized infections

Linezolid resistant Staphylococcus haemolyticus:First case report from India

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Asian Pacific Journal of Tropical Medicine 2012年第10期5卷 837-838页

作者： Varsha Gupta Shivani Garg Ruby Jain Sudhir Garg jagdish chander Department of Microbiology Government Medical College Hospital Department of Orthopedics Government Medical College Hospital

Linezolid is being increasingly used in the treatment of infections with gram-positive organisms especially methicillin resistant Staphylococcal *** resistance to this antimicrobial is emerging but it is extremely *** we document first case of linezolid resistant Staphylococcus haemolyticus(***) from *** organism was isolated from pus oozing from a postsurgical site in 61 year old male hailing from an adjoining state of Haryana.

关键词： Linezolid Staphylococcus haemolyticus Drug resistance

Dengue in pregnancy:an under-reported illness,with special reference to other existing co-infections

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 206-208页

作者： Nidhi Singla Sunita Arora Poonam Goel jagdish chander Anju Huria Departments of Microbiology Government Medical College Hospital Departments of Obstetrics & Gynaecology Government Medical College Hospital

Objective:To keep the level of awareness high as far as incidence of dengue among pregnant women is ***:A total of 300 blood samples of patients with fever in pregnancy were received in the Department of Microbiology to rule out dengue infection(January 2011 to December 2012).The samples were put up for presence of dengue IgM antibodies and NS1 Ag by *** patients who turned out to be positive for dengue serology were retrospectively analysed with respect to patient's *** *** presentation,complications,platelet counts and maternal as well as foetal ***:Out of 300 females tested.22(7.3%) were found positive for dengue infection during the said lime *** of them 9 were positive for IgM antibodies against dengue and 10 were found to be positive for NS1 *** 3 were positive for both IgM antibody and NS1 *** patients presented with dengue in first trimester.9 in second trimester and 8 in third *** patients had *** with coinfection of dengue with malaria had intrauterine death of fetus at 37 weeks while the second one having dengue with typhoid had a preterm vaginal delivery at 35 ***:Establishing diagnosis of dengue infection in pregnancy is important for effective management by the obstetricians particularly the mode of delivery due to the potential risk of hemorrhage for both the mother and the *** seen in endemic areas may be more common than usually reported.

关键词： Dengue Pregnancy Females Cointections

Spectrum of fungal keratitis: clinicopathologic study of 44 cases

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International Journal of Ophthalmology(English edition) 2014年第1期7卷 114-117页

作者： Rajpal Singh Punia Reetu Kundu jagdish chander Sudesh Kumar Arya Uma Handa Harsh Mohan Department of Pathology Government Medical College and Hospital Department of Microbiology Government Medical College and Hospital Department of Ophthalmology Government Medical College and Hospital

AIM: To determine the causative agents of fungal keratitis and study the predisposing factors over a period of ten years in a single tertiary care hospital. ·METHODS: A retrospective analysis of fungal corneal ulcers was done from 2003-2012. Patients’ clinical data were noted from the file records. Correlation of histopathological diagnosis was done with the report on fungal culture. · RESULTS: Mycotic keratitis was established in 44 cases by a positive fungal culture. Direct microscopic examination of potassium hydroxide(KOH) mounts revealed fungal elements in 39 cases while 40 cases showed fungus on Gram stained smears. Males(54.55%) were more commonly affected than the females(45.45%). The age ranged from 18 to 82 years. Most common age group to be involved was 41-60 years. Predisposing risk factors were seen in 34(77.27%) cases. Most common findings on clinical examination were anterior chamber reaction and conjunctival injection seen in all the cases. Other common findings were stromal infiltration and hypopyon seen in 20(45.45%) and 18(40.91%) cases respectively. On histopathological examination the fungus was typed,as aspergillus in 34 cases while no definite typing was possible in 10 cases. The predominant isolate was aspergillus flavus(59.09%) followed by fusarium(15.91%). Mixed fungal and bacterial infection was seen in 3(6.82%) cases. ·CONCLUSION: Although culture is the gold standard for definitive diagnosis of fungal keratitis,direct microscopic examination of corneal scrapings or histomorphological evaluation of biopsies allow a rapidpreliminary diagnosis. Early administration of antifungal treatment helps in preventing dreadful complications.

关键词： mycotic keratitis fungal culture infection cornea