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Dexamethasone inhibits hypoxia-induced epithelial-mesenchymal transition in colon cancer

World Journal of Gastroenterology 2015年第34期21卷 9887-9899页

作者： Jung Ho kim You-Jin Hwang Sang Hoon Han Young Eun Lee Saerom kim Yoon jae kim jae Hee Cho Kwang An Kwon Ju hyun kim Se-Hee kim Department of Internal Medicine Gachon University Gil Medical Center Division of Biological Science Gachon University of Medicine and Science Department of Internal Medicine Jeju National University School of Medicine Gachon Medical Research Institute Gachon University Gil Medical Center

AIM:To elucidate the effects of dexamethasone on hypoxia-induced epithelial-to-mesenchymal transition(EMT) in colon ***:Human colon cancer HCT116 and HT29 cells were exposed to normoxic(21%) and hypoxic(1%) conditions. First,the effect of dexamethasone on cell viability was examined by MTT cell proliferation assay. In order to measure the expression levels of EMT markers(Snail,Slug,Twist,E-cadherin,and integrin αVβ6) and hypoxia-related genes [Hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF)] by dexamethasone,quantitative real-time polymerase chain reaction and western blot analysis were performed. Furthermore,the morphological changes of colon cancer cells and the expression pattern of E-cadherin by dexamethasone were detected through immunocytochemistry. Finally,the effects of dexamethasone on the invasiveness and migration of colon cancer cells were elucidated using matrigel invasion,migration,and wound healing migration ***:Under hypoxia,dexamethasone treatment inhibited HIF-1α protein level and its downstream gene,VEGF m RNA level in the colon cancer cell lines,HCT116 and HT29. In addition,the presence of dexamethasone down-regulated the m RNA levels of hypoxia-induced Snail,Slug,and Twist,all transcriptional factors of EMT,as well as hypoxia-induced integrin αVβ6 protein level,a well-known EMT marker for colon cancer cells. Furthermore,reduced E-cadherin in hypoxic condition was found to be recoverable by treating with dexamethasone in both colon cancer cell lines. Similarly,under hypoxic conditions,dexamethasone restored the growth pattern and morphological phenotype reminiscent of colon cancer cells grown under normoxic conditions; dexamethasone blocked the migration and invasion of both colorectal cancer cell lines in ***:Our study suggested that dexamethasone has inhibitory effects on cell migration and invasion by suppressing EMT of colon cancer cell lines in hypoxic condition.

关键词： Dexamethasone Colon cancer Hypoxia Hypoxia-inducib

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Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus

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Asian Journal of Andrology 2009年第3期11卷 342-350页

作者： hyun Jung Park Won Young Lee Jin Hoi kim jae Hwan kim Hun Jong Jung Nam hyung kim Bo Kyung kim Hyuk Song Department of Animal Science College of Natural Science Konkuk University Chung-ju 380-701 Korea CHA Stem Cell Institute Graduate School of Life Science and Biotechnology Pochon CHA University Seoul 135-907 Korea Department of Animal Biotechnology College of Animal Biotechnology Konkuk University Seoul 143-701 Korea Occupation and Environment Department Medical School Konkuk University Chung-ju 380-704 Korea Department of Animal Science College of Agriculture Chungbuk National University Choung-ju Chung-buk 361-763 Korea Department of Physiology Medical School Konkuk University Chung-ju 380-701 Korea

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein （GFP）-expressing recombinant baculovirus （GFP-baculovirus）, in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus （CMV）-IE promoter, was used to transfect testicular cells in vitro, and for intra-tunica albuguineal injection of the interstitial tissue of the testis. GFP expression was monitored in frozen testes sections by fluorescence microscopy. Expression of GFP in testicular tissues was also assessed by reverse transcription polymerase chain reaction （RT-PCR）, and protein expression was assessed by Western blot. Testicular cells in vitro were infected efficiently by modified recombinant GFP-baculovirus. lntra-tunica albuguineal injection of GFP- baculovirus into the mouse testis resulted in a high level of GFP expression in the interstitial tissues. RT-PCR analysis clearly showed GFP gene expression in the testis, particularly interstitial tissues. Intra-tunica albuguineal injection of a modified baculovirus that encoded recombinant rat insulin-like growth factor binding protein （IGFBP）-5 resulted in an increase in IGFBP-5 in testis and semen. In conclusion, we have developed an efficient delivery system for gene expression in vivo in testicular cells, particularly cells of the interstitial tissue using intratunica albuguineal injection of a modified recombinant baculovirus. This method will be particularly relevant for application that requires gene delivery and protein expression in the testicular cells of the outer seminiferous tubule of the testis.

关键词： intra-tunica albuguineal injection recombinant baculovirus testis gene delivery

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Supercritical water oxidation for the destruction of toxic organic wastewaters:A review

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Journal of Environmental Sciences 2007年第5期19卷 513-522页

作者： VERIANSYAH Bambang kim jae-Duck Supercritical Fluid Research Laboratory Korea Institute of Science and Technology (KIST)-Department of Green Process and System EngineeringUniversity of Science and Technology (UST)39-1 Hawolgok-dongSeoungbuk-guSeoul 136-791Korea

The destruction of toxic organic wastewaters from munitions demilitarization and complex industrial chemical clearly becomes an overwhelming problem if left to conventional treatment processes. Two options, incineration and supercritical water oxidation （SCWO）, exist for the complete destruction of toxic organic wastewaters. Incinerator has associated problems such as very high cost and public resentment; on the other hand, SCWO has proved to be a very promising method for the treatment of many different wastewaters with extremely efficient organic waste destruction 99.99% with none of the emissions associated with incineration. In this review, the concepts of SCWO, result and present perspectives of application, and industrial status of SCWO are critically examined and discussed.

关键词： supercritical water oxidation toxic wastewater treatment SCWO industrial status

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Dipole-assisted carrier transport in bis(trifluoromethane) sulfonamide-treated O-ReS_(2) field-effect transistor

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Nano Research 2021年第7期14卷 2207-2214页

作者： jae Young Park SangHyuk Yoo Byeongho Park Taekyeong kim Young Tea Chun Jong Min kim Keonwook Kang Soo hyun Lee Seong Chan Jun Department of Mechanical Engineering Yonsei UniversitySeoul 03722Republic of Korea Center for Biomicrosystem Brain Science InstituteKorea Institute of Science and TechnologySeoul 02792Republic of Korea Sensor System Research Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Department of Physics Hankuk University of Foreign StudiesYongin 17035Republic of Korea Division of Electronics and Electrical Information Engineering Korea Maritime and Ocean UniversityBusan 49112Republic of Korea Electrical Engineering Division Engineering Department University of CambridgeCambridgeCB3 OFAUK

We demonstrate the dipole-assisted carrier transport properties of bis(trifluoromethane)sulfonamide(TFSI)-treated O-ReS_(2) field-effect *** ReS_(2) was compared with defect-mediated ReS_(2) to confirm whether the presence of defects on the interface enhances the interaction between O-ReS_(2) and TFSI *** to the experiment,density functional theory(DFT)calculation was performed,and the result indicated that the charge transfer between TFSI and O-ReS_(2) is more sensitive to external electric fields than that between TFSI and pristine ReS_(2).After TFSI treatment,the drain current of O-ReS_(2) FET was significantly increased up to 1,113.4 times except in the range of−0.32–0.76 V owing to Schottky barrier modulation from dipole polarization of TFSI molecules,contrary to a significant degradation in device performance in pristine ReS_(2) ***,in the treated O-ReS_(2) device,the dipole direction was highly influenced by the voltage sweep direction,generating a significant area of hysteresis in I–V and transfer characteristics,which was further verified by the surface potential ***,the dipole state was enhanced according to the wavelength of the light source and *** results indicate that TFSI-treated ReS_(2) FET has large potential for use as next-generation memristor,memory,and photodetector.

关键词： Two-dimensional material ReS_(2) field-effect transistor TFSI superacid dipole

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Laparoscopic cholecystectomy in patients with anesthetic problems

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World Journal of Gastroenterology 2013年第29期19卷 4832-4835页

作者： Bum-Soo kim Sun-hyung Joo Jin-hyun Joh jae-Woo Yi Department of Surgery School of Medicine Kyung Hee University Seoul 134-727 South Korea Departments of Surgery and Anesthesiology and Pain Medicine School of Medicine Kyung Hee University Seoul 134-727 South Korea

Laparoscopic cholecystectomy is a standard operation for benign gallbladder disease. As experience with laparoscopic cholecystectomy has increased, the procedure has become possible in patients with anesthetic problems. Patients with ankylosing spondylitis or severe kyphosis represent a challenging group to anesthesiologists and laparoscopic surgeons since these diseases are associated with difficult intubation, restrictive ventilatory defects, and cardiac problems. The relatively new approach of awake fiberoptic intubation is considered to be the safest option for patients with anticipated airway difficulties. Laparoscopic cholecystectomy is usually performed under general anesthesia but considerable difficulties in anesthetic management are encountered during laparoscopic surgery; for example, hemodynamic instability may develop in patients with cardiopulmonary dysfunction due to pneumoperitoneum and position changes during the operation. Nonetheless, regional anesthesia can be considered as a valid option for patients with gallbladder disease who are poor candidates for general anesthesia due to cardiopulmonary problems. We report three cases of laparoscopic cholecystectomy successfully performed in patients with anesthetic problems that included cardiopulmonary disease, severe kyphosis, and ankylosing spondylitis.

关键词： Laparoscopic cholecystectomy Kyphosis Ankylosing spondylitis Anesthetic problems

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Refractory lipoatrophy treated with autologous whole blood injection:A case report

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World Journal of Clinical Cases 2025年第2期13卷 33-38页

作者： Wonnam kim Jun Su Park En hyung kim Department of Pharmacology School of Korean MedicinePusan National UniversityYangsan 50612GyeongnamSouth Korea Department of Dermatology College of MedicineChungbuk National UniversityCheongju 28644ChungbukSouth Korea Department of Dermatology Chungbuk National University HospitalCheongju 28644ChungbukSouth Korea

BACKGROUND Intramuscular corticosteroid injection may cause adverse effects such as dermal and/or subcutaneous atrophy,alopecia,hypopigmentation,and *** cutaneous atrophy can spontaneously resolve,several treatment options have been suggested for this *** SUMMARY In this paper,we report a case of corticosteroid injection induced lipoatrophy treated with autologous whole blood(AWB)injection,as the condition had been unresponsive to fractional laser therapy.A 29-year-old female patient visited the dermatology clinic complaining of skin depression on her right buttock area,which had appeared six months *** had been only subtle improvement at the margins after fractional CO_(2) laser treatment;therefore,after obtaining informed consent from the patient,AWB treatment was *** month after the first AWB injection,the size and depth of the lesion had noticeably improved,and a slight improvement was also observed in *** Close observation is the initial treatment of choice for steroid induced skin atrophy;however,for patients in need of immediate cosmetic improvement,AWB injection may be a safe and cost-effective alternative.

关键词： Atrophy Corticosteroid Subcutaneous fat Autologous whole blood injection Laser therapy Case report

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Mechanisms of fibrogenesis in liver cirrhosis:The molecular aspects of epithelial-mesenchymal transition

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World Journal of Hepatology 2014年第4期6卷 207-216页

作者： Sun-jae Lee Kyung-hyun kim Kwan-Kyu Park Department of Pathology Catholic University of DaeguCollege of Medicine

Liver injuries are repaired by fibrosis and *** cause of fibrosis and diminished regeneration,especially in liver cirrhosis,is still *** transition(EMT) has been found to be associated with liver *** possibility that EMT could contribute to hepatic fibrogenesis reinforced the concept that activated hepatic stellate cells are not the only key players in the hepatic fibrogenic process and that other cell types,either hepatic or bone marrow-derived cells could contribute to this *** an initial enthusiasm for the discovery of this novel pathway in fibrogenesis,more recent research has started to cast serious doubts upon the real relevance of this phenomenon in human fibrogenetic *** debate on the authenticity of EMT or on its contribution to the fibrogenic process has become very *** overall result is a general confusion on the meaning and on the definition of several key *** aim of this article is to describe how EMT participates to hepatic fibrosis and discuss the evidence of supporting this possibility in order to reach reasonable and useful conclusions.

关键词： Epithelial-mesenchymal transition Liver Fibrosis Transforming growth factor-beta1 Biological markers

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Copper telluride with manipulated carrier concentrations for high-performance solid-state thermoelectrics

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Journal of Materials Science & Technology 2022年第34期129卷 190-195页

作者： hyun Ju Dabin Park Minsu kim Jooheon kim G.W.Woodruff School of Mechanical Engineering Georgia Institute of TechnologyAtlantaGA 30332USA School of Chemical Engineering&Materials Science Chung-Ang UniversitySeoul 06974Republic of Korea Department of Intelligent Energy and Industry Graduate SchoolChung-Ang UniversitySeoul 06974Republic of Korea Department of Advance Materials Engineering Chung-Ang UniversityAnseong 17546Republic of Korea

This study proposed a strategy for effectively diminishing the carrier concentration in Cu_(2)Te by introducing graphene sheets,Based on thermoelectric property measurements and single parabolic band modeling,the incorporated graphene effectively reduced the carrier concentration,not only enhancing the thermoelectric performance of the Cu_(2)Te/graphene composite but also substantially improving its figure of merit up to ~1.47 at 1000 K,which is 268% higher than that of pristine Cu_(2)Te,This study gives an insight into the control of carrier concentration and thermoelectric properties in Cu_(2)Te,and it could be extended to other copper chalcogenides for excellent thermoelectrics.

关键词： Thermoelectric Copper telluride Graphene Carrier concentration Single parabolic band model

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Enhancing ammonia production rates from electrochemical nitrogen reduction by engineering three-phase boundary with phosphorus-activated Cu catalysts

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Journal of Energy Chemistry 2023年第9期84卷 394-401页

作者： Jeehye kim Cho Hee Lee Yong hyun Moon Min Hee Lee Eun Hyup kim Sun Hee Choi Youn Jeong Jang jae Sung Lee School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology(UNIST)Ulsan 44919Republic of Korea Department of Chemical Engineering Hanyang UniversitySeoul 04763Republic of Korea Pohang Accelerator Laboratory(PAL) Pohang University of Science and Technology(POSTECH)Pohang 37673Republic of Korea

Electrochemical N_(2) reduction reaction(eNRR) over Cu-based catalysts suffers from an intrinsically low activity of Cu for activation of stable N_(2) molecules and the limited supply of N_(2) to the catalyst due to its low solubility in aqueous ***,we propose phosphorus-activated Cu electrocatalysts to generate electron-deficient Cu sites on the catalyst surface to promote the adsorption of N_(2) *** eNRR system is further modified using a gas diffusion electrode(GDE) coated with polytetrafluoroethylene(PTFE) to form an effective three-phase boundary of liquid water-gas N_(2)-solid catalyst to facilitate easy access of N_(2) to the catalytic *** a result,the new catalyst in the flow-type cell records a Faradaic efficiency of 13.15% and an NH_(3) production rate of 7.69 μg h^(-1) cm^(-2) at-0.2 V_(RHE),which represent 3.56 and 59.2 times increases from those obtained with a pristine Cu electrode in a typical electrolytic *** work represents a successful demonstration of dual modification strategies;catalyst modification and N_(2) supplying system engineering,and the results would provide a useful platform for further developments of electrocatalysts and reaction systems.

关键词： Electrochemical nitrogen reduction reaction Ammonia production Phosphorous modified copper electrodes Gas diffusion electrodes Three-phase boundary PTFE coating

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Effects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients

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The Journal of Biomedical Research 2019年第2期33卷 122-130页

作者： Byung Cheol kim jae-In Song Kyoung-Ha So Sang-Hwan hyun Laboratory of Veterinary Embryology and Biotechnology Veterinary Medical Center and College of Veterinary MedicineChungbuk National University Institute of Stem Cell & Regenerative Medicine Chungbuk National University

Despite their potential applications in future regenerative medicine, periodontal ligament stem cells(PDLSCs) are difficult to obtain in large amounts from patients. Therefore, maintaining sternness while expanding the cell numbers for medical use is the key to transitioning PDLSCs from the bench to the clinic. Lysophosphatidic acid(LPA), which is present in the human body and saliva, is a signaling molecule derived from phospholipids. In this study, we examined the effects of LPA on sternness maintenance in human PDLSCs. Several spindle-shaped and fibroblast-like periodontal ligament stem-like cell lines were established from PDLSC isolation. Among these cell lines, the most morphologically appropriate cell line was characterized. The expression levels of OCT4, NANOG(a stem cell marker), and CD90(a mesenchymal stem cell marker) were high. However, CD73(a negative marker of mesenchymal stem cells) expression was not observed. Notably, immunofluorescence analysis identified the expression of STRO-1, CD146(a mesenchymal stem cell marker), and sex determining region Y-box 2 at the protein level. In addition, lipid droplets were stained by Oil red O after the induction of adipogenesis for 21 days, and mineralized nodules were stained by Alizarin Red S after the induction of osteogenesis for 14 days. Alkaline phosphate staining also demonstrated the occurrence of osteogenesis. In summary, we established a human PDLSC line, which could be applied as a cell source for tissue regeneration in dental patients. However, further studies are needed to determine the detailed effects of LPA on PDLSCs.

关键词： periodontal ligament stem cell lysophosphatidic acid stemness primary cell culture

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