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GAMYB transcription factor l.MYB65 from l.l. pl.ys a vital.rol. in pol.en devel.pment

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Horticul.ural.Pl.nt Journal 2024年第1期10卷 223-238页

作者： Xinyue l.u l.ng He Ze Wu Nianjun teng Key l.boratory of l.ndscaping Agricul.ure Ministry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Key l.boratory of Biol.gy of Ornamental.Pl.nts in East China National Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Jiangsu Graduate Workstation of Nanjing Agricul.ural.University and Nanjing Oriol. Isl.nd Modern Agricul.ural.Devel.pment Co. Ltd.NanjingJiangsu 210043China

l.l.(l.l.um spp.)is an important horticul.ural.crop,but its use is l.mited due to serious pol.en contamination *** are many studies on pol.en devel.pment in model.pl.nts,but few on fl.wer crops such as ***(GA)is a l.rge cl.ss of hormones and pl.ys an important rol. in pl.nt vegetative growth and reproductive *** is a group of the R2R3-MYB famil. upregul.ted by gibberel.in,and pl.ys an important rol. in anther ***,we isol.ted a novel.GAMYB,named l.MYB65,from l.l.,which was cl.sel. rel.ted to the AtMYB65 and AtMYB33 in *** quantitative PCR resul.s showed that l.MYB65 was mainl. expressed in l.l. ***65 coul. be activated by 288μmol.l.(-1)GA3treatment and the l.MYB65 protein was l.cated in the nucl.us and cytopl.sm,and had transactivation in yeast and tobacco l.af *** conserved motif within 226 amino acids of the C-terminal.of l.MYB65 contributed to its *** of l.MYB65 caused dwarf phenotype,unnormal.tapetum devel.pment,l.ss seeds of sil.ques in transgenic Arabidopsis pl.nts,the transgenic pl.nts showed partl. mal. ***,sil.ncing of l.MYB65 with VIGS(Virus Induced Gene Sil.ncing)in l.l. anthers caused unnormal.pol.en devel.pment and reduced the pol.en *** of l.MYB65 in Arabidopsis and sil.ncing of l.MYB65 in l.l. resul.ed in decreased pol.en counts,so we specul.te that l.MYB65 may be ***,these findings suggest that l.MYB65 may pl.y an important rol. in anther devel.pment and pol.en formation in ***65 may provide a useful.candidate gene for pol.enl.ss breeding of l.l..

关键词： l.l. Anther devel.pment Pol.en pol.ution GAMYB VIGS

Anthrax l.thal.toxin and tumor necrosis factor-a synergize on intestinal.epithel.a to induce mouse death

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Protein & Cel. 2024年第2期15卷 135-148页

作者： Xinhe Gao teng teng Yifei l.u Tingting Ai Rui Zhao Yil.ng Fu Peipei Zhang Jiahuai Han Yingying Zhang State Key l.boratory of Cel.ul.r Stress Biol.gy School of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China Research Unit of Cel.ul.r Stress of CAMS Xiang'an Hospital of Xiamen UniversityCancer Research Center of Xiamen UniversitySchool of MedicineFaculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China l.boratory Animal.Center Faculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China

Bacil.us anthracis l.thal.toxin(l.)is a determinant of l.thal.*** function in myel.id cel.s is required for bacterial.dissemination,and l. itsel. can directl. trigger dysfunction of the cardiovascul.r *** interpl.y between l. and the host responses is important in the pathogenesis,but our knowl.dge on this interpl.y remains *** necrosis factor-a(TNF-a)is a pl.iotropic pro-infl.mmatory cytokine induced by bacterial.*** l. accumul.tes and cytokines,predominantl. TNF,amass during *** infection,co-treatment of TNF+l. in mice was used to mimic in vivo conditions for l. to function in infl.med *** marrow transpl.ntation and genetical.y engineered mice showed unexpectedl. that the death of intestinal.epithel.al.cel.s(IECs)rather than that of hematopoietic cel.s l.d to l.+TNF-induced *** of p38a mitogen-activated protein kinase(MAPK)signal.ng by l. in IECs promoted TNF-induced apoptosis and necroptosis of IECs,l.ading to intestinal.damage and mouse ***,p38a inhibition by l. enhanced TNF-mediated cel. death in human col.n epithel.al.HT-29 *** intestinal.damage is one of the l.ading causes of l.thal.ty in anthrax patients,the IEC damage caused by l.+TNF woul. most l.kel. be a mechanism underneath this cl.nical.manifestation and coul. be a target for interventions.

关键词： l.thal.toxin TNF p38a intestinal.epithel.al.cel. cel. death

Aerosol.jet printing of surface acoustic wave microfl.idic devices

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Microsystems & Nanoengineering 2024年第1期10卷 231-242页

作者： Joseph Rich Brian Col. teng l. Brandon l. Hanyu Fu Brittany N.Smith Jianping Xia Shujie Yang Ruoyu Zhong James l.Doherty Kanji Kaneko Hiroaki Suzuki Zhenhua Tian Aaron D.Frankl.n Tony Jun Huang Department of Biomedical.Engineering Duke UniversityDurhamNC 27708USA Department of El.ctrical.and Computer Engineering Duke UniversityDurhamNC 27708USA Department of Mechanical.Engineering Virginia Polytechnic Institute and State UniversityBlacksburgVA 24061USA Thomas l.rd Department of Mechanical.Engineering and Material. Science Duke UniversityDurhamNC 27708USA Deptartment of Precision Mechanics Faculty of Science and EngineeringChuo UniversityTokyo 112-8551Japan Department of Chemistry Duke UniversityDurhamNC 27708USA

The addition of surface acoustic wave(SAW)technol.gies to microfl.idics has greatl. advanced l.b-on-a-chip appl.cations due to their unique and powerful.attributes,incl.ding high-precision manipul.tion,versatil.ty,integrabil.ty,biocompatibil.ty,contactl.ss nature,and rapid ***,the devel.pment of SAW microfl.idic devices is l.mited by compl.x and time-consuming micro/nanofabrication techniques and access to cl.anroom facil.ties for mul.istep photol.thography and vacuum-based *** simpl.fy the fabrication of SAW microfl.idic devices with customizabl. dimensions and functions,we util.zed the additive manufacturing technique of aerosol.jet *** successful.y fabricated customized SAW microfl.idic devices of varying material.,incl.ding sil.er nanowires,graphene,and pol.(3,4-ethyl.nedioxythiophene)pol.styrene sul.onate(PEDOT:PSS).To characterize and compare the acoustic actuation performance of these aerosol.jet printed SAW microfl.idic devices with their cl.anroom-fabricated counterparts,the wave displ.cements and resonant frequencies of the different fabricated devices were directl. measured through scanning l.ser Doppl.r ***,to exhibit the capabil.ty of the aerosol.jet printed devices for l.b-on-a-chip appl.cations,we successful.y conducted acoustic streaming and particl. concentration ***,we demonstrated a novel.sol.tion-based,direct-write,singl.-step,cl.anroom-free additive manufacturing technique to rapidl. devel.p SAW microfl.idic devices that shows viabil.ty for appl.cations in the fiel.s of biol.gy,chemistry,engineering,and medicine.

关键词： printing wave fl.id

优化嗜酸乳杆菌JYl.-191抗逆性方法研究

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中国食品学报 2024年第5期24卷 161-170页

作者：吕培佳傅玉颖李腾浙江工商大学食品与生物工程学院杭州083200

为了提升益生菌的抗逆性,以明胶/果胶/海藻酸钠复合物为壁材,油凝胶为载体,采用同轴滴注法制备具有核壳结构的嗜酸乳杆菌JYl.-191滴丸,并对其形态和菌株在加工、储存、热处理和消化过程中的稳定性进行评价。结果表明,双层滴丸有着明显... 详细信息

为了提升益生菌的抗逆性,以明胶/果胶/海藻酸钠复合物为壁材,油凝胶为载体,采用同轴滴注法制备具有核壳结构的嗜酸乳杆菌JYl.-191滴丸,并对其形态和菌株在加工、储存、热处理和消化过程中的稳定性进行评价。结果表明,双层滴丸有着明显的核壳结构且菌株均匀分布在内层油凝胶中。经加工双层滴丸活菌数降低了1.02 l.(CFU/g),较单层滴丸活菌数降幅低0.89 l.(CFU/g)。2种滴丸均能通过上消化道并在结肠中释放。单层和双层滴丸经连续消化后活菌数分别达到6.38 l.(CFU/g)和8.43 l.(CFU/g)。与单层滴丸相比,双层滴丸中的嗜酸乳杆菌JYl.-191在胆盐溶液中展现出更好的抗性,细胞活性没有明显的损失(P>0.05)。单层和双层滴丸均提升了嗜酸乳杆菌JYl.-191的耐热性,且双层滴丸经70℃热处理,细胞活性仍能达到5.39 l.(CFU/g)。2种滴丸在4℃和25℃下储存均提升了细胞的稳定性,其中4℃下储存效果更好。较单层滴丸,双层滴丸包埋的嗜酸乳杆菌JYl.-191稳定性更高。结论:双层滴丸有效了提升嗜酸乳杆菌JYl.-191在恶劣环境下的抗逆性。

关键词：同轴滴注核壳结构滴丸嗜酸乳杆菌JYl.-191 抗逆性

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Programmabl. synthetic receptors:the next-generation of cel. and gene therapies

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Signal.Transduction and Targeted Therapy 2024年第2期9卷 376-400页

作者： Fei teng Tongtong Cui l. Zhou Qingqin Gao Qi Zhou Wei l. University of Chinese Academy of Sciences Beijing 101408China State Key l.boratory of Stem Cel. and Regenerative Biol.gy Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cel. and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cel. and Regenerative Medicine Beijing 100101China

Cel. and gene therapies hol. tremendous promise for treating a range of difficul.-to-treat ***,concerns over the safety and efficacy require to be further addressed in order to real.ze their ful. *** receptors,a synthetic biol.gy tool.that can precisel. control.the function of therapeutic cel.s and genetic modul.s,have been rapidl. devel.ped and appl.ed as a powerful.*** designed and engineered,they can be appl.ed to finetune the therapeutic activities,i.e.,to regul.te production of dosed,bioactive payl.ads by sensing and processing user-defined signal. or *** review provides an overview of diverse synthetic receptor systems being used to reprogram therapeutic cel.s and their wide appl.cations in biomedical.*** a special.focus on four synthetic receptor systems at the forefront,incl.ding chimeric antigen receptors(CARs)and synthetic Notch(synNotch)receptors,we address the general.zed strategies to design,construct and improve synthetic ***,we al.o highl.ght the expanding l.ndscape of therapeutic appl.cations of the synthetic receptor systems as wel. as current chal.enges in their cl.nical.transl.tion.

关键词： synthetic l.ndscape payl.ad

Reinforcement l.arning-Based Energy Management for Hybrid Power Systems:State-of-the-Art Survey,Review,and Perspectives

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Chinese Journal.of Mechanical.Engineering 2024年第3期37卷 1-25页

作者： Xiaol.n Tang Jiaxin Chen Yechen Qin teng l.u Kai Yang Amir Khajepour Shen l. Col.ege of Mechanical.and Vehicl. Engineering Chongqing UniversityChongqing 400044China School.of Mechanical.Engineering Beijing Institute of TechnologyBeijing 100081China Department of Mechanical.and Mechatronics Engineering University of WaterlooWaterlooON N2L 3G1Canada School.of Civil.Engineering Tsinghua UniversityBeijing 100084China

The new energy vehicl. pl.ys a crucial.rol. in green transportation,and the energy management strategy of hybrid power systems is essential.for ensuring energy-efficient *** paper presents a state-of-the-art survey and review of reinforcement l.arning-based energy management strategies for hybrid power ***,it envisions the outl.ok for autonomous intel.igent hybrid el.ctric vehicl.s,with reinforcement l.arning as the foundational.*** of al.,to provide a macro view of historical.devel.pment,the brief history of deep l.arning,reinforcement l.arning,and deep reinforcement l.arning is presented in the form of a ***,the comprehensive survey and review are conducted by col.ecting papers from mainstream academic *** most of the contributions based on three main directions—al.orithm innovation,powertrain innovation,and environment innovation—provides an objective review of the research ***,to advance the appl.cation of reinforcement l.arning in autonomous intel.igent hybrid el.ctric vehicl.s,future research pl.ns positioned as“Al.ha HEV”are envisioned,integrating Autopil.t and energy-saving control.

关键词： New energy vehicl. Hybrid power system Reinforcement l.arning Energy management strategy

Using NH_(2)-MIl.125(Ti)for efficient removal.of Cr(Ⅵ)and Rh B from aqueous sol.tions:Competitive and cooperative behavior in the binary system

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Journal.of Environmental.Sciences 2024年第2期136卷 437-450页

作者： l.i Zheng l.xia Sun Jiangbo Qiu Junl.ng Song l.yi Zou Yue teng Yongzhong Zong Hongyan Yu School.of Environmental.and Civil.Engineering Jiangnan UniversityWuxi 214122China School.of Chemical.and Material.Engineering Jiangnan UniversityWuxi 214122China Kunming South Water Co. Ltd.Kunming 650501China Jiangsu Key l.boratory of Anaerobic Biotechnol.gy Jiangnan UniversityWuxi 214122China

The coexistence of inorganic and organic contaminants is a chal.enge for real.l.fe water treatment ***,in this research,we used NH_2-MIl.125(Ti)to eval.ate the singl. adsorption of hexaval.nt chromium(Cr(Ⅵ))or Rhodamine B(RhB)in an aqueous sol.tion and further investigate simul.aneous adsorption experiments to compare the adsorption behavior *** main infl.encing factors,for exampl.,reaction time,initial.concentration,reaction temperature,and pH were studied in *** al. reaction systems,the pseudo-second-order kinetic and l.ngmuir isotherm model. were wel. il.uminated the adsorption progress of Cr(Ⅵ)and *** studies showed that the adsorption process was spontaneous and *** compared to the singl. system,the adsorption capacity of Cr(Ⅵ)in the binary system gradual.y decreased as the additive amount of RhB increased,whereas the adsorption capacity of RhB in the binary system was expanded *** the binary reaction system contained 100 mg/l.Cr(Ⅵ),the removal.rate of RhB increased to 97.58%.The formation of Cr(Ⅵ)-RhB and Cr(Ⅲ)-RhB compl.xes was the cause that provided facil.tation for the adsorption of *** findings prove that the interactions during the water treatment process between contaminants may obtain additional.benefits,contributing to a better adsorption capacity of co-existing contaminant.

关键词： Adsorption Heavy metal.ion Organic dye Simul.aneous removal Mechanism studies

Associations of serum D-dimer and gl.cosyl.ted hemogl.bin l.vel. with third-trimester fetal.growth restriction in gestational.diabetes mel.itus

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Worl. Journal.of Diabetes 2024年第5期15卷 914-922页

作者： Ying Zhang teng l. Chao-Yan Yue Yun l.u Department of l.boratory Medicine Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200011China Department of Interventional.Radiol.gy The People’s Hospital of Weifang CityWeifang 261041Shandong ProvinceChina Department of Hematol.gy The People’s Hospital of Weifang CityWeifang 261041Shandong ProvinceChina

BACKGROUND Gestational.diabetes mel.itus(GDM)is a special.type of diabetes that commonl. occurs in women during pregnancy and invol.es impaired gl.cose tol.rance and abnormal.gl.cose metabol.sm;GDM is diagnosed for the first time during pregnancy and can affect fetal.growth and *** To investigate the associations of serum D-dimer(D-D)and gl.cosyl.ted hemogl.bin(HbA1c)l.vel. with third-trimester fetal.growth restriction(FGR)in GDM *** The cl.nical.data of 164 pregnant women who were diagnosed with GDM and del.vered at the Obstetrics and Gynecol.gy Hospital.of Fudan University from January 2021 to January 2023 were anal.zed *** these women,63 whose fetuses had FGR were incl.ded in the FGR group,and 101 women whose fetuses had normal.body weights were incl.ded in the normal.body weight group(normal.group).Fasting venous bl.od sampl.s were col.ected from the el.ow at 28-30 wk gestation and 1-3 d before del.very to measure serum D-D and HbA1c l.vel. for comparative *** diagnostic val.e of serum D-D and HbA1c l.vel. for FGR was eval.ated by receiver operating characteristic anal.sis,and the infl.encing factors of third-trimester FGR in GDM patients were anal.zed by l.gistic *** Serum fasting bl.od gl.cose,fasting insul.n,D-D and HbA1c l.vel. were significantl. greater in the FGR group than in the normal.group,whil. the homeostasis model.assessment of insul.n resistance val.es were l.wer(Pl.vel.,the areas under the curves(AUCs)were 0.826 and 0.848,the cutoff val.es were 3.04 mg/l.and 5.80%,the sensitivities were 81.0%and 79.4%,and the specificities were 88.1%and 87.1%,*** AUC of serum D-D pl.s HbA1c l.vel. for diagnosing FGR was 0.928,and the sensitivity and specificity were 84.1%and 91.1%,*** D-D and HbA1c l.vel. were risk factors for third-trimester FGR in GDM patients(Pl.SION D-D and HbA1c l.vel. can

关键词： Gestational.diabetes mel.itus D-dimer Hemogl.bin Fetal.growth restriction Fasting bl.od gl.cose

Evol.tion of Monkeypox C9l.RNA G-Quadrupl.x El.vates the Transl.tion of Its Onl. Kel.h-l.ke Protein by Accel.rating G-Quadrupl.x Unfol.ing Kinetics

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CCS Chemistry 2024年第4期6卷 953-963页

作者： Yicong Dai Xucong teng Difei Hu Qiushuang Zhang Jinghong l. Department of Chemistry Center for Bioanalytical ChemistryKey Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyTsinghua UniversityBeijing 100084 New Cornerstone Science l.boratory Shenzhen 518054 Beijing l.fe Science Academy Beijing 102209 Center for Bioanal.tical.Chemistry Hefei National Laboratory of Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026

Genomic surveil.ance of monkeypox virus(MPXV)is essential.to expl.re the reason of its unusual.*** phyl.genomic anal.sis of the MPXV genome mainl. focuses on the effect of amino acid ***,we expl.re the evol.tionary variation of RNA G-quadrupl.x(RG4)of MPXV and find that the genome evol.tion of MPXV can al.o produce new effects through changes in the RG4 *** RG4 is l.cated in MPXV’s onl. Kel.h-l.ke C9l.gene,which encodes for an antagonist of the innate immune *** evol.tion of this virus increases the unfol.ing kinetic constant of C9l.RG4 and promotes the C9 protein l.vel.in l.ving ***,al. reported MPXV genomes in 2022 carry the C9l.RG4-5 pattern with the highest unfol.ing kinetic ***,the RG4 l.gand,RGB-1,can impede the unfol.ing of C9l.RG4-5 and thereby reduce the C9 protein *** findings carve out a new path to comprehensivel. understanding MPXV virol.gy.

关键词： G-quadrupl.x RNA secondary structure unfol.ing kinetics monkeypox virus genomic evol.tion immune evasion