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百合三系间杂种的创造及其应用潜力

百合三系间杂种的创造及其应用潜力

Analysis of the meiosis in the F_1 hybrids of Longiflorum × Asiatic(LA) of lilies(Lilium) using genomic in situ hybridization

2010中国球根花卉年会

作者：谢松林王仙芝张延龙牛立新 jaap m.van tuyl 西北农林科技大学园艺学院西北农林科技大学林学院 Plant Breeding Wageningen UR the Netherlands

为整合百合不同杂种系间优良特性,选取来自传统百合杂种系的育种材料及不同类型的百合多倍体LA、LT、LO、AT、OT及新杂种,运用常规授粉方法进行了杂交,并采用胚培养技术对幼胚进行抢救。结果表明,常规杂交及胚培养技术是创造百合多系间... 详细信息

为整合百合不同杂种系间优良特性,选取来自传统百合杂种系的育种材料及不同类型的百合多倍体LA、LT、LO、AT、OT及新杂种,运用常规授粉方法进行了杂交,并采用胚培养技术对幼胚进行抢救。结果表明,常规杂交及胚培养技术是创造百合多系间杂种的有效手段,杂交父母本之间如存在相同的"桥梁种"背景则可以显著提高获得百合三系间杂种的几率。另外,多系间杂种的获得与杂交组合及正反交密切相关。三系杂种为百合新品种的培育提供了一个育种新方向。

关键词：百合杂种桥梁种杂交

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Journal of Genetics and Genomics 2008年第11期35卷 687-695页

作者： Shujun Zhou munikote S. Ramanna Richard G.E Visser jaap m. van tuyl Institute of Landscape College of Agriculture ＆ Biotechnology Zhejiang University Hangzhou 310029 China Plant Breeding Lab Wageningen University P.O. Box 386 6700 AJ Wageningen The Netherlands

Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modem lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the FI hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F1 hybrids of Longiflorum × Asiatic （LA） hybrids in Lilium. Therefore, the meiotic process of ten F1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research. The results showed that： at metaphase I, the homoeologous chromosome pairing among different F1 hybrids ranged from 2.0 to 11.4 bivalents formed by homoeologous chromosomes per pollen mother cell （PmC）, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PmCs; at anaphase I, all biva- lents were disjoined and most univalents were divided. Both the disjoined bivalents （half-bivalents） and the divided univalents （sister chromatids） moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents, were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only ex

关键词： Lilium genomic in situ hybridization abnormal meiosis crossover 2n gamete

45S rDNA在4种百合属植物染色体上的物理定位

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园艺学报 2008年第6期35卷 859-862页

作者：周树军 jaap van tuyl 臧德奎夏宜平李方浙江大学农业与生物技术学院杭州310029 Plant Breeding Plant Science Group Wageningen University P.O. Box 16 Wageningen 6700 AA The Netherlands 山东农业大学林学院山东泰安271018

利用荧光原位杂交的技术将45SrDNA在麝香百合(Lilium longiflorum),柠檬色百合(***),天香百合(***)和豹纹百合(***)的染色体上进行了定位。结果表明:45S rDNA在这4种植物中都分布于染色体的着丝点附近,但其位点数量、所在的染色体和信... 详细信息

利用荧光原位杂交的技术将45SrDNA在麝香百合(Lilium longiflorum),柠檬色百合(***),天香百合(***)和豹纹百合(***)的染色体上进行了定位。结果表明:45S rDNA在这4种植物中都分布于染色体的着丝点附近,但其位点数量、所在的染色体和信号的强弱有很大的变化。其中,麝香百合和豹纹百合中各有3对染色体在着丝点附近有45S rDNA的信号,柠檬色百合和天香百合中各有4对染色体在着丝点附近有45S rDNA的信号。但是天香百合的两对中部染色体的着丝点附近都有45S rDNA的信号,柠檬色百合的两对中部染色体中只有一条有45S rDNA的信号,而麝香百合和豹纹百合中没有任何一条有45S rDNA的信号。由于这4种百合在百合属中分别属于不同组,其45S rDNA的位点数量、所在染色体和信号强弱的变化为这种分组的合理性提供了一些分子生物学的证据。

关键词：百合 rDNA 荧光原位杂交(FISH) 核型染色体

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东方百合×喇叭百合杂种配子的育性

福建农林大学学报（自然科学版） 2015年第6期44卷 601-605页

作者：罗建让 van tuyl jaap 梁振旭西北农林科技大学风景园林艺术学院陕西杨凌712100 瓦格宁根大学植物育种系

以常见的15个东方百合×喇叭百合(OT百合)品种和14个东方百合品种为材料,通过花粉离体萌发及常规柱头授粉,研究OT百合的花粉活力及其与东方百合雌配子的亲和性;同时,以这15个OT百合品种为母本,以‘布莱斯卡’、‘蒙特祖玛’和‘圣坦德’... 详细信息

以常见的15个东方百合×喇叭百合(OT百合)品种和14个东方百合品种为材料,通过花粉离体萌发及常规柱头授粉,研究OT百合的花粉活力及其与东方百合雌配子的亲和性;同时,以这15个OT百合品种为母本,以‘布莱斯卡’、‘蒙特祖玛’和‘圣坦德’3个东方百合品种为父本,采用常规柱头授粉研究OT百合雌配子的育性.结果表明:(1)OT百合品种‘宇宙山’和‘入侵’的花粉具有低频率的活力(花粉萌发率分别为5%和6%),其他13个OT百合品种的花粉基本没有活力;(2)‘宇宙山’的花粉与东方百合品种‘卡西尼’、‘居里’、‘凯里湖’的雌配子具有一定亲和性;(3)‘入侵’的花粉与东方百合品种‘吉瑞斯’、‘科德萨’的雌配子具有一定亲和性;(4)除‘可可茶’、‘曼妮莎’、‘震动’和‘耶罗林’4个OT百合品种外,其他11个OT百合品种的雌配子均具有一定程度的育性.

关键词：百合雌配子雄配子育性

糖尿病相关的足病的定义和标准(2023年更新版)

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感染、炎症、修复 2024年第1期25卷 80-85页

作者： jaap J.van Netten Sicco A.Bus Jan Apelqvist Pam Chen Vivienne Chuter Robert Fitridge Frances Game Robert J.Hinchliffe Peter A.Lazzarini Joseph mills matilde monteiro-Soares Edgar J.G.Peters Katherine m.Raspovic Eric Senneville Dane K.Wukich Nicolaas C.Schaper on behalf of the International Working Group on the Diabetic Foot 张会峰(译) 许樟荣(审校) Department of Rehabilitation medicine Amsterdam UMCLocation University of AmsterdamAmsterdamthe Netherlands Amsterdam movement Sciences Program RehabilitationAmsterdamthe Netherlands Department of Endocrinology University Hospital of MalmöMalmöSweden Joondalup Health Campus Ramsay HealthcareSydneyAustralia Faculty of Health University of TasmaniaHobartAustralia School of Health Sciences Western Sydney UniversitySydneyAustralia Faculty of Health and medical Sciences University of AdelaideAdelaideAustralia Vascular and Endovascular Service Royal Adelaide HospitalAdelaideAustralia Department of Diabetes and Endocrinology University Hospitals of Derby and Burton NHS Foundation TrustDerbyUK Department of Vascular Surgery University of BristolBristolUK School of Public Health and Social Work Queensland University of TechnologyBrisbaneAustralia Allied Health Research Collaborative The Prince Charles HospitalBrisbaneAustralia michael E.DeBakey Department of Surgery Division of Vascular Surgery and Endovascular TherapyBaylor College of MedicineHoustonTexasUSA Portuguese Red Cross School of Health–Lisbon LisbonPortugal mEDCIDS–Departamento de medicina da Comunidade Informação e Decisão em Saúde Faculty of Medicine of the University of PortoPortoPortugal RISE@CINTESIS Faculty of Medicine Oporto UniversityPortoPortugal Department of Internal medicine Amsterdam UMCVrije Universiteit AmsterdamSection of Infectious DiseasesAmsterdamthe Netherlands Department of Orthopaedic Surgery University of Texas Southwestern Medical CenterDallasTexasUSA Department of Infectious Diseases Gustave Dron Hospital Tourcoing Univ-lilleTourcoingFrance Div.Endocrinology MUMC+CARIM and CAPHRI InstituteMaastrichtThe Netherlands 不详河南省人民医院内分泌科河南郑州450003 战略支援部队特色医学中心北京100101

糖尿病相关的足病管理涉及多个学科,通用的专业词汇对于各学科间准确沟通交流至关重要。国际糖尿病足工作组(IWGDF)对相关文献进行了系统综述,构成了制定IWGDF指南的基础,并且明确了糖尿病相关的足病的一系列定义、标准及名词解释。本... 详细信息

糖尿病相关的足病管理涉及多个学科,通用的专业词汇对于各学科间准确沟通交流至关重要。国际糖尿病足工作组(IWGDF)对相关文献进行了系统综述,构成了制定IWGDF指南的基础,并且明确了糖尿病相关的足病的一系列定义、标准及名词解释。本文介绍2023年IWGDF对这些定义和标准的更新,并建议在临床实践和研究中一致使用,以利于世界各地不同学科人员针对糖尿病相关的足病的专业性交流。

关键词：定义糖尿病糖尿病相关的足病国际糖尿病足工作组

Nasopharyngeal carcinoma in Indonesia:epidemiology,incidence,signs,and symptoms at presentation

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Chinese Journal of Cancer 2012年第4期31卷 185-196页

作者： marlinda Adham Antonius N.Kurniawan Arina Ika muhtadi Averdi Roezin Bambang Hermani Soehartati Gondhowiardjo I Bing Tan jaap m.middeldorp Department of Otorhinolaryngology Department of Anatomy Pathology Department of Radiotherapy Faculty of MedicineUniversity of IndonesiaDr.Cipto Mangunkusumo HospitalJakartaIndonesia Antoni van Leeuwenhoek Hospital Netherlands Cancer Institute Department of Pathology VU University Medical CenterAmsterdamNetherlands

Among all head and neck (H&N) cancers,nasopharyngeal carcinoma (NPC) represents a distinct entity regarding epidemiology,clinical presentation,biological markers,carcinogenic risk factors,and prognostic *** is endemic in certain regions of the world,especially in Southeast Asia,and has a poor *** Indonesia,the recorded mean prevalence is 6.2/100 000,with 13 000 yearly new NPC cases,but otherwise little is documented on NPC in ***,we report on a group of 1121 NPC patients diagnosed and treated at *** mangunkusumo Hospital,Jakarta,Indonesia between 1996 and *** studied NPC incidence among all H&N cancer cases (n=6000) observed in that period,focusing on age and gender distribution,the ethnic background of patients,and the disease *** also analyzed most prevalent signs and symptoms and staging of NPC patients at first *** this study population,NPC was the most frequent H&N cancer (28.4%),with a male-to-female ratio of 2.4,and was endemic in the Javanese ***,NPC appeared to affect patients at a relatively young age (20% juvenile cases) without a bimodal age ***,NPC initiated in the fossa of Rosenmuller and spreaded intracranially or locally as a mass in the ***,NPC developed at the submucosal level spreading outside the anatomic limits of the *** presentation,NPC associated with hearing problems,serous otitis media,tinnitus,nasal obstruction,anosmia,bleeding,difficulty in swallowing and dysphonia,and even eye symptoms with diplopia and *** initial diagnosis is difficult to make because early signs and symptoms of NPC are not specific to the ***-age Epstein-Barr virus (EBV) infection combined with frequent exposure to environmental carcinogenic co-factors is suggested to cause NPC *** NPC is the most frequent histological type and is closely associated with *** of the EBV-encoded latent membrane protein

关键词：早期症状鼻咽癌发病率 Epstein-Barr病毒全国人民代表大会体征流行病学印尼

magnetically actuated glaucoma drainage devicefor regulating intraocular pressure afterimplantation

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microsystems & Nanoengineering 2023年第4期9卷 91-105页

作者： Inês C.F.Pereira Ralph J.S.van mechelen Hans m.Wyss Leonard Pinchuk Henny J.m.Beckers jaap m.J.den Toonder microsystems Department of Mechanical EngineeringEindhoven University of Technology5600MB EindhovenThe Netherlands Institute for Complex molecular Systems(ICmS) Eindhoven University of Technology5600MB EindhovenThe Netherlands University Eye Clinic maastricht Maastricht University Medical Centre+(MUMC+)6202AZ MaastrichtThe Netherlands InnFocus Inc.a Santen CompanyMiamiFlorida 33186USA Ophthalmic Biophysics Center Bascom Palmer Eye InstituteUniversity of Miami Miller School of MedicineMiamiFlorida 33136USA

The key risk factor for glaucoma is increased intraocular pressure (IOP). Glaucoma drainage devices implanted in theeye can reduce IOP and thus stop disease progression. However, most devices currently used in clinical practice arepassive and do not allow for postsurgical IOP control, which may result in serious complications such as hypotony (i.e.,excessively low IOP). To enable noninvasive IOP control, we demonstrate a novel, miniature glaucoma implant that willenable the repeated adjustment of the hydrodynamic resistance after implantation. This is achieved by integrating amagnetic microvalve containing a micropencil-shaped plug that is moved using an external magnet, thereby openingor closing fluidic channels. The microplug is made from biocompatible poly(styrene-block-isobutylene-block-styrene)(SIBS) containing iron microparticles. The complete implant consists of an SIBS drainage tube and a housing elementcontaining the microvalve and fabricated with hot embossing using femtosecond laser-machined glass molds. Usingin vitro and ex vivo microfluidic experiments, we demonstrate that when the microvalve is closed, it can providesufficient hydrodynamic resistance to overcome hypotony. Valve function is repeatable and stable over time. Due to itssmall size, our implant is a promising, safe, easy-to-implant, minimally invasive glaucoma surgery device.

关键词： enable implantation overcome

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郁金香基腐病抗性QTL分析

农业科技与信息(现代园林) 2015年第4期 337-338页

作者：唐楠 Theo van der Lee J.m.van tuyl P.Arens 青海大学高原花卉研究中心 Plant Research International Wageningen University and Research Centre Plant Breeding Wageningen University and Research Centre

郁金香基腐病由尖孢镰刀菌（Fusarium oxysporum ***）引起,严重影响郁金香种球和切花生产。农艺防治措施如避免机械损伤、发现病株及时去除并销毁、实行轮作等,防治效果较差、效率较低,因而生产上主要依靠大量使用化学药剂进行控制。... 详细信息

郁金香基腐病由尖孢镰刀菌（Fusarium oxysporum ***）引起,严重影响郁金香种球和切花生产。农艺防治措施如避免机械损伤、发现病株及时去除并销毁、实行轮作等,防治效果较差、效率较低,因而生产上主要依靠大量使用化学药剂进行控制。长期连续的使用化学药剂对生态环境造成严重威胁,并极有可能使病原菌对杀菌剂产生抗性。因此,选育具有抗基腐病的郁金香品种成为近年来的主要育种目标。郁金香的生长周期长达4;年,采用常规杂交育种选育新品种的过程非常缓慢。通过分子标记辅助选择,可显著加速郁金香的育种过程。但是由于郁金香的基因组太大（1C≈30GB）,已发表的分子标记数目非常有限,尚无可参照的遗传图谱,阻碍了分子标记辅助选择育种的发展。本研究试料为郁金香品种‘Kees Nelis’与‘Cantata’杂交得到的F1代植株。采用土壤感病法及接种法对作图群体及亲本的基腐病抗性进行评价。土壤感病是传统的抗病性鉴定方法,将郁金香种球栽种在受病原菌感染的土壤中,培养6周后开始观察参照品种的感病程度,当感病参照完全发病时,采用1;级标准对其它测试种球进行分级,以每个基因型所有重复的平均值代表其抗病性。接种法是使用携带绿色荧光蛋白（Green Fluorescence Protein,GFP）基因的病原菌菌株,对待测种球进行穿刺接种,利用GFP产生的荧光信号对感病程度进行定量。利用多种分子系统标记对作图群体进行基因型检测,通过软件Join map4.1,采用双拟假测交策略,分别对两亲本进行连锁分析,构建双亲的遗传连锁图谱。利用软件map QTL6,分年度、分表型鉴定方法对郁金香抗基腐病进行QTL定位。结果表明:研究发现基腐病抗性在两个郁金香亲本间差异显著,在杂交F1代中呈连续分布,为数量性状遗传。杂交F1代的表型分布符合正态分布,且存在明显的超亲分离。两年土壤感病鉴定结果的相关性较差,2011年土壤感病鉴定和GFP-tagged ***接种鉴定相关性较差,2012年土壤感病鉴定和GFP-tagged ***接种鉴定相关性较高。结合3组表型鉴定数据,依次通过Kruskal-Wallis检验、Im和mQm作图,共检测到6个与郁金香基腐病抗性相关的QTL位点,不同表型鉴定试验中各QTL的显著性不同,单个QTL对表型变异的最大贡献率为12.5%;0.7%。

关键词：美郁金香基腐病 QTL

The chemokine receptor CCR7 is a promising target for rheumatoid arthritis therapy

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Cellular & molecular Immunology 2019年第10期16卷 791-799页

作者： Georgios L.moschovakis Anja Bubke michaela Friedrichsen Jasmin Ristenpart jaap Willem Back Christine S.Falk Elisabeth Kremmer Reinhold Forster Institute of Immunology Hannover Medical SchoolCarl-Neuberg Str.130625 HannoverGermany Pepscan Therapeutics BV LelystadThe Netherlands Institute of Transplant Immunology Integrated Research and Treatment Center TransplantationIFB.TxHannover Medical SchoolCarl-Neuberg Str.130625 HannoverGermany Helmholtz-Zentrum münchen Institute of Molecular ImmunologyD-81377 MunichGermany Present address:Biozentrum martinsried Dept.Bio II.LMU MünchenGrosshaderner Str.2D-82152 MartinsriedGermany

The chemokine receptor CCR7 and its ligands CCL19 and CCL21 guide the homing and positioning of dendritic and T cells in lymphoid organs,thereby contributing to several aspects of adaptive immunity and immune *** the present study,we investigated the role of CCR7 in the pathogenesis of collagen-induced arthritis(CIA).By using a novel anti-human CCR7 antibody and humanized CCR7 mice,we evaluated CCR7 as a target in this autoimmune model of rheumatoid arthritis(RA).Ccr7-deficient mice were completely resistant to CIA and presented severely impaired antibody responses to collagen II(CII).Selective CCR7 expression on dendritic cells restored arthritis severity and anti-CII antibody *** and therapeutic treatment of humanized CCR7 mice with anti-human CCR7 mAb 8H3-16A12 led to complete resistance to CIA and halted CIA progression,*** data demonstrate that CCR7 signaling is essential for the induction of CIA and identify CCR7 as a potential therapeutic target in RA.

关键词： CCR7 impaired immunity