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Evaluation of Cuban Bacillus thuringiensis(Berliner,1911)(Bacillales:Bacillacea)isolates with larvicidal activity against Aedes aegypti(Linnaeus,1762)(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 79-86页

作者： Aileen Gonzalez Rizo Camilo E Castañet Martinez Celeste Ramírez Cardentey Ariamys Companioni Ibañez Zulema Menéndez Díaz Lianet Monzote Fidalgo Hilda M HernandezÁlvarez Vector Control Department Institute of Tropical MedicinePedro KouríAutopistaNovia del Mediodía km 6½La Habana 11400Cuba Virology Department Institute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba Parasitology Department Institute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba

Objective:To evaluate 11 Cuban native Bacillus(B.)thuringiensis isolates in order to select one with the best larvicidal activity against Aedes(Ae.)aegypti and low ***:The cry and cyt genes of the isolates(A21,A51,L95,L910,M29,R84,R85,R87,R89,U81 and X48)were amplified by *** influence of organic matter and NaCl on the larvicidal activity was tested by *** was assayed on peritoneal macrophages of BALB/c ***:The cyt1(Aa,Ab,Ba),cyt2,cry4aA,cry4Ba,cry11(Aa,Ba,Bb)and cry10 genes were identified in all native Cuban *** larvicidal activity(LC_(90))of seven isolates was affected by the presence of organic matter in the water,while A21,A51,L910,R84,U81 and X48 had better LC_(50),LC_(90),LC_(95) than the 266/29-Ⅶ-98 control *** LC_(50) of two isolates was affected by the presence of NaCl and A21,A51,R85 isolate had better larvicidal activity than the 266/29-Ⅶ-98 control *** terms of toxicity against macrophages,the extracts of nine isolates were less cytotoxic than the control ***:Native isolate A21 had the main virulence factors against *** larvae,displayed a good larvicidal activity in presence of different factors related with *** breeding sites,and had low citotoxicity against *** results can contribute to the improvement of existing biological control strategies and the development of new biolarvicides.

关键词： Mosquitoes Biological control agent Bacillus thuringiensis Bioassays Aedes aegypti cry and cyt genes

The Challenge of Implementing Information Security Standards in Small and Medium e-Business Enterprises

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Journal of Software Engineering and Applications 2014年第10期7卷 883-890页

作者： ja’far Alqatawna King Abdulla II School of Information Technology The University of Jordan Amman Jordan

The dynamic nature of online systems requires companies to be proactive with thwarting information security threats, and to follow a systematic way for managing and evaluating the security of their online services. The existence of security standards is an important factor that helps organisations to evaluate and manage security by providing guidelines and best practices that enable them to follow a standard and systematic way to protect their e-Business activities. However, the suitability of available information security standards for Small and Medium e-Business Enterprises (e-SME) is worth further investigation. In this paper three major security standards including Common Criteria, System Security Engineering-Capability and Maturity Model and ISO/IEC 27001 were analysed. Accordingly, several challenges associated with these standards that may render them difficult to be implemented in e-SME have been identified.

关键词： Information Security Standards e-Business Small and Medium Enterprises

Congenital absence of the splenic artery and splenic vein accompanied with a duodenal ulcer and deformity

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World Journal of Gastroenterology 2009年第11期15卷 1401-1403页

作者： Eun Kyung Shin Won Moon Seun ja Park Moo In Park Kyu Jong Kim Jee Suk Lee Jin Hwan Kwon Department of Gastroenterology Kosin University College of MedicineBusanSouth Korea Department of Radiology Kosin University College of MedicineBusanSouth Korea

Congenital absence of the splenic artery is a very rare *** the best of our knowledge, congenital absence of the splenic artery accompanied with absence of the splenic vein has not been *** report a case of the absence of the splenic artery and vein in a 61-year-old woman who presented with postprandial epigastric discomfort. Upper gastrointestinal endoscopy showed a dilated, pulsatile vessel in the fundus and duodenal stenosis. An abdominal computed tomography(CT)scan revealed absence of the splenic vein with a tortuously engorged gastroepiploic ***-dimensional CT demonstrated the tortuously dilated left gastric artery and the left gastroepiploic artery with non-visualization of the splenic *** administration of a proton pump inhibitor,abdominal symptoms resolved without any recurrence of symptoms during 6 mo of follow-up.

关键词：脾动脉脾静脉十二指肠溃疡先天性消化道内窥镜检查电脑断层扫描上腹部不适胃网膜静脉

Progress and records in the study of endogenetic mineralization during collisional orogenesis

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Chinese Science Bulletin 2000年第1期45卷 1-10页

作者： Yanjing Chen Huayong Chen Yulin Liu Guangjun Guo Yong Lai Shan Qin Baoling Huang Zengjie Zheng Yinghui Sui Chao Li et al. Peking Univ Dept Geol Beijing 100871 Peoples R China

To develop and perfect the theory of plate tectonics and regional metallogeny, metal-logenesis during collisional orogenesis should be thoroughly studied and will attract increasing at-tention of more and more scientists. This paper presents the main aspects of research and discus-sions on metallogenesis during collisional orogenesis after the development of plate tectonics, and accordingly divides the study history into two stages, i.e. the junior stage during 1971-1990 and the senior stage after 1990. Beginning with the negation of mineralization in the collision regime by Guild (1971), the focus of study was put on whether there occurred any mineralization during collisional orogenesis at the junior stage. At the senior stage, which is initiated by the advance of met-allogenic and petrogenic model for collisional orogenesis, scientists begin to pay their attention to the geodynamic mechanism of metallogenesis, spatial and temporal distribution of ore deposits, ore-forming fluidization, relationship

关键词： collisional orogenesis metallogenesis study history progress frontier.

Trichoderma *** Bacillus *** growth promoters in maize(Zea mays L.)

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 37-46页

作者： López-Valenzuela BE Armenta-Bojórquez AD Hernández-Verdugo S Apodaca-Sánchez MA Samaniego-Gaxiola ja Valdez-Ortiz A Universidad Autónoma de Sinaloa.Facultad de Agricultura del Valle del Fuerte.Avenida japaraqui y Calle 16 s/n Juan JoséRíosSinaloaC.P 81110México Universidad Autónoma de Baja California.Instituto de Ciencias Agrícolas.Carretera a Delta s/n Ejido Nuevo leónBaja CaliforniaC.P.21705México Instituto Politécnico Nacional.Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa.Blvd.Juan de Dios Bátiz Paredes#250 Colonia San JoachinGuasaveSinaloaC.P.81101México Universidad Autónoma de Sinaloa Facultad de Agronomía.Km.17.5Carretera Culiacán-El DoradoCuliacán RosalesSinaloaC.P.80000México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Campo Experimental La LagunaBlvd.JoséSantos Valdez 1200 Pte.MatamorosCoahuilaC.P.27440México Universidad Autónoma de Sinaloa Facultad de Ciencias Químico Biológicas.Calzada de las Américas y Calle Universitarioss/n.Ciudad UniversitariaCuliacánSinaloaC.P.80013México

Microbes that are beneficial to plants are used to enhance the crop growth,yield and are alternatives to chemical *** and Bacillus are the predominant plant growth-promoting fungi and *** objective of this study was select,characterize,and evaluate isolates of Trichoderma *** Bacillus *** from the northern region of Sinaloa,Mexico,and assess their effect on growth promotion in maize(Zea mays L.).In greenhouse conditions,four Trichoderma isolates and twenty Bacillus isolates,as well as two controls,were tested in a completely randomized design with three *** selected the two best strains of Trichoderma and Bacillus:TB=Trichoderma asperellum,TF=Trichoderma virens,B14=Bacillus cereus sensu lato and B17=Bacillus cereus,which were evaluated in the field in a completely randomized blocks in factorial arrangement design with three replicates applying different rates of nitrogen fertilizer(0,150 kg N/ha,and 300 kg N/ha).Treatments 5(B17=***)and 11(TF=***)both fertilized with 150 kg N/ha showed similar yields and they did not reveal significant differences from the treatments fertilized with 300 kg N/*** indicated that treatment 5(B17=*** with 150 kg N/ha)and treatment 11(TF=*** with 150 kg N/ha)were efficient as growth promoters,by not showing significant differences in root volume and dry weight of *** results indicated a reduction of 50% in the rate of nitrogen to fertilizer required for maize(Zea mays L.)*** microorganisms Trichoderma and Bacillus could be an alternative to reduce the use of chemical fertilizers in maize.

关键词： Trichoderma Bacillus Native strains Biofertilizers

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对足球守门员扑救球点球时机的研究

上海体育学院学报 1991年第2期15卷 5-8,12+96页

作者：王钢周荣根上海体育学院球类系足球教研室

点球攻守是当今足球比赛中决定胜负的重要因素之一。本文通过大量数据分析推导得出,守门员在防守点球时的最佳扑救(反应)时机,应该选择在看到主罚队员最后一步助跑支撑脚上步离开地面的一瞬间,这样可以获得最佳的提前量。

关键词：足球守门员球点球扑救时机

Cost-effective screening using a two-antibody panel for detecting mismatch repair deficiency in sporadic colorectal cancer

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World Journal of Clinical Cases 2021年第24期9卷 6999-7008页

作者： Jong Beom Kim Young Il Kim Yong Sik Yoon Jihun Kim Seo Young Park Jong Lyul Lee Chan Wook Kim In ja Park Seok-Byung Lim Chang Sik Yu Jin Cheon Kim Division of Colon and Rectal Surgery University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea Department of Pathology University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea Department of Clinical Epidemiology and Biostatistics University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea

BACKGROUND The microsatellite instability(MSI)test and immunohistochemistry(IHC)are widely used to screen DNA mismatch repair(MMR)deficiency in sporadic colorectal cancer(CRC).For IHC,a two-antibody panel of MLH1 and MSH2 or four-antibody panel of MLH1,MSH2,PMS2,and MSH6 are *** general,MSI is known as a more accurate screening test than *** To compare two-and four-antibody panels of IHC in terms of accuracy and cost benefit on the basis of MSI testing for detecting MMR *** We retrospectively analyzed patients with CRC who underwent curative surgery between 2015 and 2017 at a tertiary referral *** IHC with four antibodies and MSI tests were routinely *** sensitivity and specificity of a fourand two types of two-antibody panels(PMS2/MSH6 and MLH1/MSH2)were compared on the basis of MSI testing for detecting MMR *** High-frequency MSI was found in 5.5%(n=193)of the patients(n=3486).The sensitivities of the four-and two types of two-antibody panels were 97.4%,92.2%,and 87.6%,*** specificities of the three types of panels did not differ significantly(99.6%for the four-antibody and PMS2/MSH6 panels,99.7%for the MLH1/MSH2 panel).Based on Cohen's kappa statistic(κ),four-and twoantibody panels were in almost perfect agreement with the MSI test(κ>0.9).The costs of the MSI test and the four-and two-antibody panels of IHC were approximately$200,$160,and$80,*** Considering the cost of the four-antibody panel IHC compared to that of the twoantibody panel IHC,a two-antibody panel of PMS2/MSH6 might be the best choice in terms of balancing cost-effectiveness and accuracy.

关键词： Adenocarcinoma DNA mismatch repair Immunochemistry Monoclonal antibody Microsatellite instability Cost-effectiveness

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室内外空气污染对儿童免疫指标的影响

环境与健康杂志 1989年第4期6卷 1-4页

作者：王瑾陈秉衡上海医科大学环卫教研室

本文选择了不同大气污染环境下以及家庭使用不同燃料的小学生,检测其免疫功能。综合PHA皮试、唾液溶菌酶含量,外用血T淋巴细胞转化功能,血清IgG、A、M的结果,大气轻污染区煤饼组的小学生免疫功能受抑制最明显,大气重污染区煤气组的小学... 详细信息

本文选择了不同大气污染环境下以及家庭使用不同燃料的小学生,检测其免疫功能。综合PHA皮试、唾液溶菌酶含量,外用血T淋巴细胞转化功能,血清IgG、A、M的结果,大气轻污染区煤饼组的小学生免疫功能受抑制最明显,大气重污染区煤气组的小学生次之,大气轻污染区煤气组的小学生免疫功能最好。计算了三组小学生颗粒物的时间加权平均接触量分别为1.108,0.462和0.220mg/m^3。对影响小学生免疫功能变化因素的贡献率分析,发现室内燃料性质是最重要因素,大气污染其次。

关键词：室内污染大气污染免疫功能儿童

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HPLC法测定毛果芸香碱色谱条件的筛选

西北药学杂志 1996年第2期11卷 51-52页

作者：钱春梅 Lin,et 西安医科大学药学系

探讨了利用正相硅胶－反相洗脱系统测定毛果芸香碱的ＨＰＬＣ色谱条件。结果表明，流动相（乙腈为主体）中的含水量、磷酸与三乙胺的用量、ｐＨ都程度不同地改变该药物的保留时间。最佳色谱条件是：检测波长２１７ｎｍ，流动相为含０．... 详细信息

探讨了利用正相硅胶－反相洗脱系统测定毛果芸香碱的ＨＰＬＣ色谱条件。结果表明，流动相（乙腈为主体）中的含水量、磷酸与三乙胺的用量、ｐＨ都程度不同地改变该药物的保留时间。最佳色谱条件是：检测波长２１７ｎｍ，流动相为含０．００５％磷酸的８０％乙腈（以１ｍｏｌ／ＬＮａＯＨ调节ｐＨ＝５）。

关键词：毛果芸香碱 HPLC法筛选