检索结果-南通市图书馆

Chemoselective labeling-based spermatozoa glycan imaging reveals abnormal glycosylation in oligoasthenotspermia

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第4期35卷 475-480页

作者： Lijia Xu Tong Zhong Wei Zhao Bing Yao Lin Ding huangxian ju state key laboratory of analytical chemistry for life science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Department of Reproductive Medical Center Jinling HospitalSchool of MedicineNanjing UniversityNanjing 210002China chemistry and Biomedicine Innovation Center(ChemBIC) Nanjing UniversityNanjing 210023China Center of Clinical laboratory science The Affiliated Cancer Hospital of Nanjing Medical University&Jiangsu Cancer Hospital&Jiangsu Institute of Cancer ResearchNanjing 210009China

Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive disorders is unclear, mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation. We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia) and N-acetyl-D-galactosamine(Gal/GalNAc). We further proposed a “tandem glycan chemoselective labeling” strategy to achieve simultaneous imaging of two types of glycans on spermatozoa. We applied the developed method to the spermatozoa from oligozoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/Gal NAc expression than the normal groups. Moreover, spermatozoa from diabetic mice showed a severe decrease in number, viability, and forward motility, suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia. Our work provides a research tool to reveal the relationship between glycosylation modification and spermatozoa quality, and a promising clue for the development of glycan-based reproductive markers.

关键词： Spermatozoa Glycan Chemoselective labeling Oligoasthenotspermia Fluorescence imaging

Optical applications of quantum dots in biological system

在线全文

学校读者我要写书评

暂无评论

science China chemistry 2011年第8期54卷 1177-1184页

作者： WANG Wei & ZHU jun-Jie state key laboratory of analytical chemistry for life science School of chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China 1. state key laboratory of analytical chemistry for life science School of chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

This review presents a simple introduction on the unique properties and general synthesis of quantum dots (QDs) in which we lay emphasis on the optical applications in the biological system. The detection of biological molecules such as DNA, protein and enzyme, the cell-based analysis and in vivo animal imaging are mainly discussed.

关键词： quantum dots optical application biological system

Magnetic particles and cadmium sulfide nanoparticles tagging for signal-amplifying detection of nucleic acids

在线全文

学校读者我要写书评

暂无评论

science China chemistry 2011年第8期54卷 1304-1310页

作者： DONG XiaoYa, ZHAO WeiWei, XU Jingjuan & CHEN HongYuan state key laboratory of analytical chemistry for life science School of chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China 1. state key laboratory of analytical chemistry for life science School of chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.

关键词： CdS nanoparticles square wave stripping voltammetry nicking endonuclease electrochemical DNA assay

Polymer-assisted nanoparticulate contrast-enhancing materials

在线全文

学校读者我要写书评

暂无评论

science China chemistry 2010年第3期53卷 479-486页

作者： DING Yin1,3,HU Yong2,WU Wei1 & JIANG XiQun1 1laboratory of Mesoscopic chemistry and Department of Polymer science and Engineering,College of chemistry & Chemical Engineering,Nanjing University,Nanjing 210093,China 2National laboratory of Solid state Microstructure,Department of Material science and Engineering,Nanjing University,Nanjing 210093,China 3key laboratory of analytical chemistry for life science,Ministry of Education,School of chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,China 1. laboratory of Mesoscopic chemistry and Department of Polymer science and Engineering College of Chemistry & Chemical Engineering Nanjing University Nanjing 210093 China3. Key Laboratory of Analytical Chemistry for Life Science Ministry of Education School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. National Laboratory of Solid State Microstructure Department of Material Science and Engineering Nanjing University Nanjing 210093 China

The application of nanotechnology in medicine research has significant potential in modern biomedical research,disease diagnosis and *** fluorophore-based detection techniques have been widely used as imaging and signal transduction tools for the detection of trace levels of *** photosensitivity of the fluorophores,however,limits their application in such complex environments as living bio-systems where degradation or photobleaching *** nanoparticles have unique and stable optical,electrical,magnetic and catalytic properties because of their various shapes,sizes and *** potential of the nanoparticles as novel imaging agents has been studied as a possible solution to this *** modification of inorganic nanoparticles could enhance their biostability in physiological conditions and ability of targeting bioactive molecules.

关键词： contrast agent cell image gold nanoparticles iron oxide nanoparticles carbon nanotubes polymeric-inorganic hybrid nanoparticles

Synthesis and Crystal Structure of [Cis-1,10phenanthroline-palladium(Ⅱ)(THF)_2]dinitrate (THF = Tetrahydrofuran)

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural chemistry 2003年第5期22卷 547-549页

作者： HUANG Hui YU Shu-Yan② (state key laboratory of Polymer Physics and chemistry, Joint laboratory of Polymer science and Materials, Institute of chemistry, The Chinese Academy of sciences, Beijing 100080, China) Institute of chemistry The Chinese Academy of Sciences State Key Laborator6y of Polymer Physics and Chemistry Joint Laboratory of Polymer Science and Materials CAS 北京 100080

A mononuclear complex, cis-1,10-phenanthroline-palladium(Ⅱ)(THF)2]dinitrate (THF = tetrahydrofuran) [Pd(C10H8N2)(C4H8O)2](NO3)2 1, has been prepared and structurally characterized by single-crystal X-ray diffraction. The crystal belongs to monoclinic system, space group P21/c with a = 7.881(1), b = 27.371(3), c = 10.178(1) ? ?= 103.313(3)? V = 2136.5(4) 3, Mr = 554.83, Dc = 1.725 g/cm3, Z = 4, F(000) = 1128 and (MoK) = 0.926 mm-1. The structure was refined to R = 0.0678 and wR = 0.1651 for 1687 observed reflections with I > 2(I). It is revealed that the central Pd(Ⅱ) ion in the title complex is bonded to two THF and two phenanthroline molecules in a cis-configuration to form the coordination geometry, deviating slightly from the square plane.

关键词： crystal structure Pd(Ⅱ) complex 1,10-phenanthroline

Direct electrochemical detection of PCR product based on charge transfer through DNA

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

science Bulletin 2005年第15期 132-134页

作者： ZHAO Hongtao, ZHANG Zhijie & ju huangxian key laboratory of analytical chemistry for life science, Department of chemistry, Nanjing University, Nanjing 210093, China

Recent studies on chemistry of novel μ-CO-containing butterfly Fe/E (E=S,Se,Te) cluster salts

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

science China chemistry 2009年第1期52卷 1-14页

作者： SONG LiCheng Department of chemistry, state key laboratory of Elemento-Organic chemistry, Nankai University, Tianjin 300071, China 1. Department of chemistry State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071 China

This article describes recent developments in chemical study on a series of butterfly-shaped μ-CO-containing Fe/E (E = S, Se, Te) cluster salts. These salts include eleven novel cluster anions, which are the single butterfly one μ-CO-containing [(μ-RE)(μ-CO)Fe2(CO)6]- (A), the double butterfly two μ-CO-containing {[(μ-CO)Fe2(CO)6]2(μ-EZE-μ)}2- (B, E = S; C, E = Se), the triple butterfly three μ-CO- containing {[(μ-CO)Fe2(CO)6]3[(μ-SCH2CH2)3N]}3- (D), {[(μ-CO)Fe2(CO)6]3[1,3,5-(μ-SCH2)3C6H3]}3- (E), {[(μ- CO)Fe2(CO)6]3[(μ-SCH2)3CMe]}3- (F), the double butterfly one μ-CO-containing {[(μ-CO)Fe2(CO)6] [Fe2(CO)6][(μ-SCH2)3CMe]} - (G) derived in situ from F, the quadruple butterfly four μ-CO-containing {[(μ- CO)Fe2(CO)6]4[1,2,4,5-(μ-SCH2)4C6H2]}4- (H), the triple butterfly two μ-CO-containing {[(μ-CO)Fe2(CO)6]2 [Fe2(CO)6][1,2,4,5-(μ-SCH2)4C6H2]}2- (I) derived in situ from H, the quadruple butterfly four μ-CO-con- taining {[(μ-CO)Fe2(CO)6]4[(μ-SCH2)4C]}4- (J), and the triple butterfly two μ-CO-containing {[(μ-CO)Fe2 (CO)6]2[Fe2(CO)6][(μ-SCH2)4C]}2- (K) derived in situ from J. This article describes not only the synthetic methods for formation of such anionic cluster (A-K) salts, but also their novel reactions leading to various new types of butterfly Fe/E cluster complexes. All these findings described in this article are important both theoretically and practically.

关键词： butterfly cluster synthesis structure reaction mechanism

RHEOLOGICAL BEHAVIOR OF EPOXY RESIN WATERBORNE DISPERSIONS

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer science 2001年第5期19卷 471-476页

作者： Zhen-zhong Yang Yuan-ze Xu De-lu Zhao state key laboratory of Polymer Physics & chemistry, Center for Molecular science Institute of chemistry, Chinese Academy of sciences, Beijing 100080 China Chinese Acad Sci Inst Chem State Key Lab Polymer Phys & Chem Ctr Mol Sci Beijing 100080 Peoples R China

The waterborne dispersions of epoxy resin were prepared by the phase inversion emulsification technique. Rheological behavior and its relationship with the structural change of the systems were studied. It was shown that the concentrated dispersions were highly viscoelastic and pseudoplastic, which was attributed to the formation of a physical network among the waterborne particles via hydrogen bond. The dilute dispersions were Newtonian fluids. The discrete clusters composed of small waterborne particles were found in diluted dispersions. With increasing solid content, there existed a structural transition via percolation through a cluster-cluster aggregation mode to form the physical network, which was qualitatively evidenced by the TEM morphologies.

关键词： epoxy resin waterborne dispersion rheological behavior cluster-cluster aggregation percolation transition

SOLID PHASE TRANSITION OF SYNDIOTACTIC POLYSTYRENE IN SUPERCRITICAL CO_2

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer science 2002年第3期20卷 191-196页

作者： Yu-ying Li Jia-song He state key laboratory of Engineering Plastics, Center for Molecular science, Institute of chemistry Chinese Academy of sciences, Beijing 100080 China Chinese Acad Sci Inst Chem Ctr Mol Sci State Key Lab Engn Plast Beijing 100080 Peoples R China

Solid phase transition of the a form crystals to the 3 form crystals in syndiotactic polystyrene (sPS) samples has occurred in supercritical CO2. This transformation is different from those detected under other conditions. The effects of some factors (e.g. time, temperature, and pressure) on the solid phase transformation of sPS in supercritical CO2 were analyzed in detail. Experimental results show that longer time, higher temperature or higher pressure favors the transformation of the a form crystals to the beta form crystals.

关键词： solid phase transition syndiotactic polystyrene supercritical CO2