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Transplanted choroidal plexus epithelial cells can integrate with organotypic spinal cord slices into a new system

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BIOCELL 2022年第6期46卷 1537-1544页

作者： jingjie liu XIAOYAN DING LI XIANG SHENGLI HUANG Department of Neurology The Second Affiliated HospitalCollege of MedicineXi’an Jiaotong UniversityXi’an710004China Department of Ophthalmology Xi’an No.3 HospitalXi’an710018China Department of Orthopedics The Second Affiliated HospitalCollege of MedicineXi’an Jiaotong UniversityXi’an710004China

This study aimed to evaluate the integration of transplanted choroidal plexus epithelial cells with organotypic spinal cord *** spinal cord slices,normally cultured for 6 days,were divided into control group(Ctrl)and transplanted group(T).The choroidal plexus epithelial cells were dissociated and primary cultured(C group).The choroidal plexus epithelial cells cultured for 6–7 days were labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanineperchlorate(CM-Dil),and were identified by transthyretin(TTR)in *** were adjusted to the density of 0.5–1×107/ml,then 2μl cells suspension were transplanted to the spinal cord slices in the T *** same amount of basal medium was dripped on the spinal cord slices in the Ctrl *** 14 days of transplantation,the differentiations into neurons and astrocytes,and the synapses were identified by immunofluorescence *** the same time,the ratios of cell differentiations and synapses in new system,and the changes of MAPK signaling pathway were tested by western *** choroid plexus epithelial cells were well labeled by CM-Dil and were immune-stained by TTR in *** choroid plexus epithelial cells bodies were small when transplanted on the spinal cord slices,but big when transplanted on the polyester membrane *** transplanted cells could differentiate into astrocytes,and possibly differentiate into neurons,and there were a large number of synaptophysin positive vesicles between transplanted cells and organotypic spinal cord slices in immunofluorescence *** levels of GFAP,TUB-III and synaptophysin in the T group were higher than which in the Ctrl and C groups in western blotting(P<0.05).And the ratios of p-JNK/JNK and p-P38/P38 in the T group were significantly lower than which in the Ctrl and C groups(P<0.05).But the ratio of p-ERK/ERK in the three groups was of no significant *** transplanted choroidal plexus epi

关键词： Cell transplantation Choroidal plexus epithelial cells Integration Organotypic spinal cord slices

Theoretical Calculation of the Real Vapor Pressure of Al during ISM Processing of Ni-xAl (***) (x=25-50) Alloy

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Journal of Materials Science & Technology 2004年第2期20卷 189-192页

作者： jingjie GUO,Guizhong liu, Yanqing SU, Jun JIA and Hengzhi FUSchool of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China Sch. of Mat. Sci. and Eng. Harbin Inst. of Technol. Harbin 150001 China

A new model was established to calculate the real vapor pressure of Al in the molten Ni-xAl （***）（x=25;0） alloy. The effects of the holding time, chamber pressure, mole fraction of Al and melting temperature on the real vapor pressure of Al in the vacuum chamber were analyzed. Because of the impeding effect of the real vapor pressure on the evaporation loss rate, within a short time （less than 10 s）, the real vapor pressure tends to a constant value. When the chamber pressure is less than the saturated vapor pressure of Al, the real vapor pressure of Al is equal to the chamber pressure. While when the chamber pressure is higher than the saturated vapor pressure, the real vapor pressure of Al approaches to the saturated vapor pressure of Al of the same condition.

关键词： Real vapor pressure Volatile elements ISM process Ni-Al alloy

Critical and Impeding Pressures of Al Evaporation during ISM Processing of NiAl

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Journal of Materials Science & Technology 2003年第6期19卷 571-575页

作者： Guizhong liu, jingjie GUO, Yanqing SU, Jingzhe GUO, Jun JIA and Hengzhi FUSchool of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China Sch. of Mat. Sci. and Eng. Harbin Inst. of Technol. Harbin 150001 China

In this paper, the critical pressure pcrit and impeding pressure pimpe of the elemental evaporation were defined and studied based on the calculation of the relationship between the evaporation loss rate Nm and the chamber pressure p during melting of NiAl alloys. When the chamber pressure is lower than pcrit or higher than Pimpe Nm tends to be the maximum or minimum value and remains almost unchanged. However, declines sharply with the increase of the chamber pressure when pcrit

关键词： ISM process Al evaporation Critical pressure Impeding pressure

贝叶斯电流分解:利用单个传感器感知家用电器电流

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计算机研究与发展 2018年第3期55卷 662-672页

作者：刘晶杰聂磊计算机体系结构国家重点实验室(中国科学院计算技术研究所) 北京100190 中国科学院大学北京100049

通过单个传感器对家庭中各个电器的用电行为进行感知是普适计算中的一个重要应用,其关键问题是电流分解,即在给定总电流波形情况下计算各个电器的实际电流.此问题现有2类求解方法:稳态估计方法和线性分解方法.前一类方法基于电器稳态耗... 详细信息

通过单个传感器对家庭中各个电器的用电行为进行感知是普适计算中的一个重要应用,其关键问题是电流分解,即在给定总电流波形情况下计算各个电器的实际电流.此问题现有2类求解方法:稳态估计方法和线性分解方法.前一类方法基于电器稳态耗电假设,使用稳态波形估计电器的工作电流.虽然该类方法能避免电器间的相互干扰,但是其结果不能反映总电流的实时变化.后一类方法通过模型约束或数据约束对电流波形进行线性降维,之后将总电流分解到各个低维线性空间中.虽然其分解结果能够反映总电流的实时变化,但是相似电器会降低分解结果的精度.从贝叶斯统计的角度将上述方法的关键假设松弛为位置向量先验分布与噪音先验分布,并提出了基于这2个分布的贝叶斯电流分解方法.利用真实用电数据,构造了多组仿真实验对此方法进行评测.实验结果表明:提出方法分解精度高于原有2类方法,其感知结果既能够反映总电流及各个电器电流的实时变化,又能够降低相似电器对分解结果的干扰.

关键词：家庭用电单点感知电流分解普适计算贝叶斯方法

Coarsening Manner and Microstructure Evolution of Al-In Hypermonotectic Alloy during Rapidly Cooling Process

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Journal of Materials Science & Technology 2002年第3期18卷 241-244页

作者： Yuan liu, jingjie GUO, Jun JIA, Yanqing SU, Hongsheng DING, Jiuzhou ZHAO and Xiang XUESchool of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China [ Manuscript received April 13, 2001, in revised form June 2,5, 2001] Harbin Inst Technol Sch Mat Sci & Engn Harbin 150001 Peoples R China

A numerical model reflecting the real physical processes well has been developed to predict the coarsening manner of the second phase droplets and the microstructural evolution under the common action of nucleation, diffusional growth, colliding coagulation during rapid cooling Al-ln hypermonotectic alloys. The model reflects the real physical processes well and is also applicable to other immiscible alloys.

关键词： Al-In immiscible alloy Coarsening Microstructure

Prosperity and Disappearance of Introduced Fishes in Xiangmoshan Reservoir

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Journal of Northeast Agricultural University(English Edition) 2010年第3期17卷 29-37页

作者： FAN Zhaoting CHEN Weixing liu Yi FANG jingjie liu Qingzhen College of Animal Sciences and Technology Northeast Agricultural University Harbin 150030 China

In order to enhance the resources, some fishes were introduced into Xiangmoshan reservoir. The introduced fishes consisted of different ecotypes such as silver carp （Hypophthahnichthys rnolitrix （Cuvier et Valenciennes））, big head （Aristichthys nobilis （Richardso）） and lake herring （Coregonus clupeaformis （Mitchill）） that were plankton feeders, common carp （Cyprinus carpio Linnaeus） and crucian carp （Carassius auratus gibelio） belonging to the polyphagia, and also the non-native predator Walleye （Stizostedion vitreum （Mitchill）） and big mouth （Silurus soldatovi rneridonalis Chen）, et al. Among there were not only the cool water fish Peled （Coregonus peled （Gmelin））, but also the warm water fish big mouth. The introduction of non-native fishes resulted in ecosystem changes of the reservoir and some native fishes had gone to decrease their population sizes or been in the condition of depopulation such as Opsariichthys uncirostris arnurensis （Berg）, Rhodeus sericeus （Pallas） and Silurus asotus （Linnaeus）. The native common carp had disappeared after the introduction of non-native species. Roach （Rutilus rutilus lacustris （Pallas） had been thriven for one or two generations after its introduction and immeidiatly disappeared. Silver fish （Protosalanx hyalocranius （Abbott））, Lake herring （Coregonus clupeaformis （Mitchill）） and Walleye （Stizostedion vitreum （Mitchill）） died several years after the introduction, so most of the introduced species has passed out of existence. Via many years introduction of different fishes, composition of species and the structure of the population in the reservoir had been changed obviously. It was necessary to further estimate whether the ecosystem of the reservoir had been damaged.

关键词： Xiangmoshan reservoir introdued fish prosperity and disappearance ecological damage

Methods and Countermeasures of Controlling Drilling Cost under New Normal

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Energy and Power Engineering 2015年第13期7卷 584-590页

作者： Haixu Qu He Huang Haiqing Cui jingjie liu Liang Zhao School of Petroleum Engineering Northeast Petroleum University Daqing China Economic Evaluation Center for Exploration and Development of Liaohe Oilfield Company Panjin China Special Oil Company of Liaohe Oilfield Panjin China Research Institute of Petroleum Exploration and Development Beijing China

The oil price drops in the international crude oil market and the benefit of upstream operations reduces which has been the new normal of oil companies so far. The upstream operations production and management of oil companies face numerous problems and challenges. One of the most significant aspects is to control and optimize investment. Drilling investment is an important component of development investment in drilling oil and gas wells. This paper proposes ways and countermeasures of controlling drilling cost from 3 aspects which are technology innovation, management innovation and system innovation on basis of studying drilling cost structure and influence factors analysis. The results have some guidance and exemplary significance to show how to improve production and management effect respectively on upstream operations of oil companies under new normal.

关键词： New Normal Drilling Cost Upstream Operations Investment Innovation

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基于模糊原理的中间件自适应优化

微计算机信息 2007年第5X期23卷 235-237页

作者：刘竞杰安徽工贸职业技术学院计算机系

J2EE中间件系统得到了广泛的使用。为了提高服务性能,需要配置系统参数。传统的手工配置法费时费力,而且在负载变化的环境中不能自适应改变,造成了性能随时间越来越低下。为此,我们提出了基于模糊控制J2EE应用服务器自适应调优系统。系... 详细信息

J2EE中间件系统得到了广泛的使用。为了提高服务性能,需要配置系统参数。传统的手工配置法费时费力,而且在负载变化的环境中不能自适应改变,造成了性能随时间越来越低下。为此,我们提出了基于模糊控制J2EE应用服务器自适应调优系统。系统实时监控性能数据,并基于模糊决策器,动态改变参数,优化性能。通过测试表明,该系统能减少响应时间,提高系统性能。

关键词：性能调优模糊控制 J2EE

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带宽测量技术中背景流量特征和影响

微计算机信息 2007年第18期23卷 145-146,138页

作者：刘竞杰安徽工贸职业技术学院计算机系

端到端的带宽测量技术一直是研究的热点。现在有很多测量带宽的方法和工具,但是它们的测试环境特别是背景流量的设置,都是不相同的。首先调查了各种方法提出的测试环境;然后通过实验证明了不同的背景流量会影响到测试结果;最后提出了生... 详细信息

端到端的带宽测量技术一直是研究的热点。现在有很多测量带宽的方法和工具,但是它们的测试环境特别是背景流量的设置,都是不相同的。首先调查了各种方法提出的测试环境;然后通过实验证明了不同的背景流量会影响到测试结果;最后提出了生成公平背景流量的新方法。该方法具有实际网络流量的特性,也便于在模拟环境中实现,可作为测量带宽算法的评估标准。

关键词：带宽检测背景流量