Transplanted choroidal plexus epithelial cells can integrate with organotypic spinal cord slices into a new system

作     者：JINGJIE LIU XIAOYAN DING LI XIANG SHENGLI HUANG 

作者机构：Department of NeurologyThe Second Affiliated HospitalCollege of MedicineXi’an Jiaotong UniversityXi’an710004China Department of OphthalmologyXi’an No.3 HospitalXi’an710018China Department of OrthopedicsThe Second Affiliated HospitalCollege of MedicineXi’an Jiaotong UniversityXi’an710004China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第6期

页      面：1537-1544页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：This study was supported by National Natural Science Foundation of China(81471247) 

主　　题：Cell transplantation Choroidal plexus epithelial cells Integration Organotypic spinal cord slices 

摘      要：This study aimed to evaluate the integration of transplanted choroidal plexus epithelial cells with organotypic spinal cord *** spinal cord slices,normally cultured for 6 days,were divided into control group(Ctrl)and transplanted group(T).The choroidal plexus epithelial cells were dissociated and primary cultured(C group).The choroidal plexus epithelial cells cultured for 6–7 days were labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanineperchlorate(CM-Dil),and were identified by transthyretin(TTR)in *** were adjusted to the density of 0.5–1×107/ml,then 2μl cells suspension were transplanted to the spinal cord slices in the T *** same amount of basal medium was dripped on the spinal cord slices in the Ctrl *** 14 days of transplantation,the differentiations into neurons and astrocytes,and the synapses were identified by immunofluorescence *** the same time,the ratios of cell differentiations and synapses in new system,and the changes of MAPK signaling pathway were tested by western *** choroid plexus epithelial cells were well labeled by CM-Dil and were immune-stained by TTR in *** choroid plexus epithelial cells bodies were small when transplanted on the spinal cord slices,but big when transplanted on the polyester membrane *** transplanted cells could differentiate into astrocytes,and possibly differentiate into neurons,and there were a large number of synaptophysin positive vesicles between transplanted cells and organotypic spinal cord slices in immunofluorescence *** levels of GFAP,TUB-III and synaptophysin in the T group were higher than which in the Ctrl and C groups in western blotting(P0.05).And the ratios of p-JNK/JNK and p-P38/P38 in the T group were significantly lower than which in the Ctrl and C groups(P0.05).But the ratio of p-ERK/ERK in the three groups was of no significant *** transplanted choroidal plexus epi