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Excessive ammonia inhibited transcription of MsU2 gene and furthermore affected accumulation distribution of allantoin and amino acids in alfalfa Medicago sativa

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Journal of Integrative Agriculture 2015年第7期14卷 1269-1282页

作者： WANG Li jiang lin-lin Nomura Mika Tajima Shigeyuki CHENG Xian-guo College of Life Science Shanxi Normal University Key Laboratory of Plant Nutrition and Fertilizers Agriculture of Ministry/Insititute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences Faculty of Agriculture Kagawa University 2393 Ikenobe Miki-choKita-gun Kagawa 761-0795 Japan

In legume plants, uricase gene （Nodulin-35） plays a positive role in metabolism of ureide and amide compounds in symbiotic nitrogen-fixing in the nodules. In this study, a pot experiment was performed to examine the effects of ammonium application on the transcription of MsU2 gene and distribution of major nitrogen compounds in alfalfa Medicago sativa. Data showed that alfalfa plant has a significant difference in contents of nitrogen compounds in xylem saps compared with soybean plant, and belongs to typical amide type legume plants with little ureide accumulation, and the accumulation of asparagines and ureide in the tissues of alfalfa is mainly gathered in the nodules. Northern blotting showed that excessive ammonium significantly inhibited the transcription of MsU2 gene in the nodules and roots, and mRNA accumulation of MsU2 gene in the plants exposed to excessive ammonium decreased gradually with culture time extension, indicating that application of ammonium significantly inhibited the transcription of MsU2 gene in the alfalfa plants. Although the application of exces- sive ammonium increased the contents of amino acids in various tissues of alfalfa, the accumulation of allantoin reflecting the strength of uricase activity is remarkably reduced in the xylem saps, stems and nodules when alfalfa plants exposed to excessive ammonium, suggesting that application of excessive ammonium generated a negative effect on symbiosis fixing-nitrogen system due to inhibition of ammonium ion on uricase activity in the nodules of alfalfa. This result seems to imply that application of excessive ammonium in legume plants should not be proposed to avoid affecting the ability of fixing nitrogen in the nodules of legume plants, and reasonable dose of ammonium should be recommended to effectively utilize the fixed N from atmosphere in legume plant production.

关键词： alfalfa amino acids ammonium MsU2 gene allantoin

Thellungiella halophila ThPIP1 gene enhances the tolerance of the transgenic rice to salt stress

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Journal of Integrative Agriculture 2015年第10期14卷 1911-1922页

作者： QIANG Xiao-jing YU Guo-hong jiang lin-lin SUN lin-lin ZHANG Shu-hui LI Wei CHENG Xian-guo Key Lab of Plant Nutrition and Fertilizers Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences College of Land and Environment Shenyang Agricultural University

Aquaporin proteins were demonstrated to play an important regulatory role in transporting water and other small molecules. To better understand physiological functions of aquaporins in extremophile plants, a novel ThPIP1 gene from the Thellungiella halophila was isolated and functionally characterized in the transgenic rice. Data showed that the ThPIP1 protein encoded 284 amino acids, and was identified to be located on the plasma membrane. The expression of ThPIP1 gene in the shoots and roots of T. halophila seedlings were induced by high salinity. The transgenic rice overexpressing ThPIP1 gene significantly increased plants tolerance to salt stress through the pathway regulating the osmotic potentials, accumulation of organic small molecules substances and the ratio of K＋/Na＋ in the plant cells. Moreover, split-ubiquitin yeast two-hybrid assay showed that Th PIP1 protein specifically interacted with ThPIP2 and a non-specific lipid-transfer protein 2, suggesting that ThPIP1 probably play a key role in responding to the reactions of multiple external stimulus and in participating in different physiological processes of plants exposed to salt stress.

关键词： ThPIP1 transgenic rice salt stress protein interaction Thellungiella halophila

Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

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Journal of Integrative Agriculture 2017年第3期16卷 713-724页

作者： jiang lin-lin HAN Guang-ming LAN Yu LIU Sai-nan GAO Ji-ping YANG Xu MENG Jun CHEN Wen-fu Liaoning Biochar Engineering & Technology Research Center Shenyang Agricultural University Shenyang 110866 P.R.China Rice Research Institute Shenyang Agricultural University Shenyang 110866 P.R.China Industrial Crops Institute Hubei Academy of Agricultural Sciences Wuhan 430064 P.R.China

Biochar has been shown to influence soil microbial communities in terms of their abundance and ***,the relationship among microbial abundance,structure and C metabolic traits is not well studied under biochar *** it was hypothesized that the addition of biochar with intrinsic properties（i.e.,porous structure）could affect the proliferation of culturable microbes and the genetic structure of soil bacterial *** the meantime,the presence of available organic carbon in biochar may influence the C utilization capacities of microbial community in Biolog Eco-plates.A pot experiment was conducted with differenct biochar application（BC）rates：control（0 t ha^-1）,BC1（20 t ha^-1）and BC2（40 t ha^-1）.Culturable microorganisms were enumerated via the plate counting *** diversity was examined using denaturing gradient gel electrophoresis（DGGE）.Microbial capacity in using C sources was assessed using Biolog *** addition of biochar stimulated the growth of actinomyces and bacteria,especially the ammonifying bacteria and azotobacteria,but had no significant effect on fungi *** phylogenetic distribution of the operational taxonomic units could be divided into the following groups with the biochar addition：Firmicutes,Acidobacteria,Gemmatimonadetes,Actinobacteria,Cyanobacteria andα-,β-,γ-andδ-Proteobacteria（average similarity〉95%）.Biochar application had a higher capacity utilization for L-asparagine,Tween 80,D-mannitol,L-serine,γ-hydroxybutyric acid,N-acetyl-D-glucosamine,glycogen,itaconic acid,glycyl-L-glutamic acid,α-ketobutyricacid and putrescine,whereas it had received decreased capacities in using the other 20 carbon sources in Biolog *** analysis（RDA）revealed that the physico-chemical properties,indices of bacterial diversity,and C metabolic traits were positively correlated with the appearance of novel sequences under BC2 *** study indicates that the addition of biochar can incre

关键词： biochar soil microbial community DGGE Biolog Eco-plates

Offload Strategy for Edge Computing in Satellite Networks Based on Software Defined Network

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Computers, Materials & Continua 2025年第1期82卷 863-879页

作者： Zhiguo Liu Yuqing Gui lin Wang Yingru jiang Communication and Network Laboratory Dalian UniversityDalian116622China College of Environment and Chemical Engineering Dalian UniversityDalian116622China

Satellite edge computing has garnered significant attention from researchers;however,processing a large volume of tasks within multi-node satellite networks still poses considerable *** sharp increase in user demand for latency-sensitive tasks has inevitably led to offloading bottlenecks and insufficient computational capacity on individual satellite edge servers,making it necessary to implement effective task offloading scheduling to enhance user *** this paper,we propose a priority-based task scheduling strategy based on a Software-Defined Network(SDN)framework for satellite-terrestrial integrated networks,which clarifies the execution order of tasks based on their ***,we apply a Dueling-Double Deep Q-Network(DDQN)algorithm enhanced with prioritized experience replay to derive a computation offloading strategy,improving the experience replay mechanism within the Dueling-DDQN ***,we utilize the Deep Deterministic Policy Gradient(DDPG)algorithm to determine the optimal resource allocation strategy to reduce the processing latency of *** results demonstrate that the proposed d3-DDPG algorithm outperforms other approaches,effectively reducing task processing latency and thus improving user experience and system efficiency.

关键词： Satellite network edge computing task scheduling computing offloading

Virulence of Salmonella enterica Serovar Enteritidis Isolate LN-248-0 and Immune Responses in BALB/c Mice

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Biomedical and Environmental Sciences 2020年第8期33卷 628-632页

作者： LIU Yang LI You Zhi FENG Tao GONG Pi Qian JIN Ying Cai ZHU Hong Wei jiang lin lin ZHANG Jian Long CHEN Guo Zhong YU Xin ZHANG Xing Xiao School of Life Science Ludong UniversityYantai 264025ShandongChina Yantai Key Laboratory of Animal Pathogenetic Microbiology and Immunology Yantai 264025ShandongChina Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Animal Products Jinan 250022ShandongChina

Salmonella enterica serovar Enteritidis(***)is a major etiological agent of acute gastroenteritis in humans;the mode of infection being consumption of undercooked or improperly handled poultry meat or contaminated eggs or milk[1,2].Among more than 2,600 serotypes of Salmonella,*** frequently causes Salmonella infections in humans[3].

关键词： handle agent properly

Wafer‑Scale Vertical 1D GaN Nanorods/2D MoS_(2)/PEDOT:PSS for Piezophototronic Effect‑Enhanced Self‑Powered Flexible Photodetectors

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Nano-Micro Letters 2025年第3期17卷 102-116页

作者： Xin Tang Hongsheng jiang Zhengliang lin Xuan Wang Wenliang Wang Guoqiang Li State Key Laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640People’s Republic of China Department of Electronic Materials School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510640People’s Republic of China

van der Waals(vdW)heterostructures constructed by lowdimensional(0D,1D,and 2D)materials are emerging as one of the most appealing systems in next-generation flexible ***,hand-stacked vdW-type photodetectors are not compatible with large-areaarray fabrication and show unimpressive performance in self-powered ***,vertical 1D GaN nanorods arrays(NRAs)/2D MoS_(2)/PEDOT:PSS in wafer scale have been proposed for self-powered flexible photodetectors arrays *** as-integrated device without external bias under weak UV illumination exhibits a competitive responsivity of 1.47 A W^(−1)and a high detectivity of 1.2×10^(11)Jones,as well as a fast response speed of 54/71μs,thanks to the strong light absorption of GaN NRAs and the efficient photogenerated carrier separation in type-II ***,the strain-tunable photodetection performances of device have been ***,the device at−0.78%strain and zero bias reveals a significantly enhanced photoresponse with a responsivity of 2.47 A W^(−1),a detectivity of 2.6×10^(11)Jones,and response times of 40/45μs,which are superior to the state-of-the-art self-powered flexible *** work presents a valuable avenue to prepare tunable vdWs heterostructures for self-powered flexible photodetection,which performs well in flexible sensors.

关键词： Vertical nanorod arrays van der Waals heterostructure Piezophototronic effect Self-powered photodetection Flexible sensors

Multi-model ensemble learning for battery state-of-health estimation:Recent advances and perspectives

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Journal of Energy Chemistry 2025年第1期100卷 739-759页

作者： Chuanping lin Jun Xu Delong jiang Jiayang Hou Ying Liang Zhongyue Zou Xuesong Mei State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong University Shaanxi Key Laboratory of Intelligent Robots School of Mechanical Engineering Xi'an Jiaotong University Department of Electrical Engineering and Automation Luoyang Institute of Science and Technology School of Mechanical Engineering Hangzhou Dianzi University

The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational performance. Despite numerous data-driven methods reported in existing research for battery SOH estimation, these methods often exhibit inconsistent performance across different application scenarios. To address this issue and overcome the performance limitations of individual data-driven models,integrating multiple models for SOH estimation has received considerable attention. Ensemble learning(EL) typically leverages the strengths of multiple base models to achieve more robust and accurate outputs. However, the lack of a clear review of current research hinders the further development of ensemble methods in SOH estimation. Therefore, this paper comprehensively reviews multi-model ensemble learning methods for battery SOH estimation. First, existing ensemble methods are systematically categorized into 6 classes based on their combination strategies. Different realizations and underlying connections are meticulously analyzed for each category of EL methods, highlighting distinctions, innovations, and typical applications. Subsequently, these ensemble methods are comprehensively compared in terms of base models, combination strategies, and publication trends. Evaluations across 6 dimensions underscore the outstanding performance of stacking-based ensemble methods. Following this, these ensemble methods are further inspected from the perspectives of weighted ensemble and diversity, aiming to inspire potential approaches for enhancing ensemble performance. Moreover, addressing challenges such as base model selection, measuring model robustness and uncertainty, and interpretability of ensemble models in practical applications is emphasized. Finally, future research prospects are outlined, specifically noting that deep learning ensemble is poised to advance ens

关键词： Contrastive Learning

CaH2-promoted activity of Ni-carbonate interface for CO2 methanation

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Journal of Energy Chemistry 2025年第1期100卷 522-532页

作者： Jin-Peng Wang Guo-Cui Mao Hui-lin jiang Bao-Xia Dong Yun-Lei Teng School of Chemistry and Chemical Engineering Yangzhou University

Transition metal-carbonate interfaces often act as active sites in heterogeneous catalytic *** interface between transition metal and metal carbonate exhibits a dynamic equilibrium during the CO2hydrogenation reaction,involving surface carbonate hydrogenation and ***,there have been few reports on engineering the activity of the interface between transition metal and alkaline earth metal carbonate for catalytic *** work demonstrated that the incorporation of CaH2in Ni/CaCO3enhances the CO2methanation activity of the *** CO2conversion for Ni/CaH2-CaCO3reached 68.5%at 400°C,which was much higher than that of the Ni/CaCO3（31.6%） and Ni/CaH2-CaO （42.4%） ***,the Ni/CaH2-CaCO3catalysts remained stable during the stability test for 24 h at 400°C and 8 *** research revealed that CaH2played a crucial role in promoting the activity of the Ni-carbonate interface for ***2could modify the electronic structure of Ni and tune the structural properties of CaCO3to generate medium basic sites （OH groups）,which are favorable for the activation of H2and ***-situ Fourier transform infrared spectroscopy （FTIR） analysis combined with density functional theory calculations demonstrated that CO2activation occurs at the hydroxyl group （OH） on the CaH2-modified Ni-carbonate surface,leading to the formation of CO3H****,our study has confirmed that CO2methanation over the Ni/CaH2-CaCO3catalysts proceeds via the formate pathway.

关键词： CO 2 methanation Metal hydride Ni catalysts Carbonate Metal-carbonate interface

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框-筒结构建筑物的折叠爆破拆除

爆炸与冲击 2009年第4期29卷 380-384页

作者：杨国梁杨军姜琳琳北京理工大学爆炸科学与技术国家重点实验室北京100081 中国矿业大学(北京)力学与建筑工程学院北京100083

采用共节点分离式钢筋混凝土模型,对框-筒结构折叠倒塌进行了三维数值模拟,包括底部起爆和顶部起爆2种方式,并且在底部起爆方式中,采用了不同切口高度和延迟时间。对结构的倒塌过程、支撑立柱的破坏过程进行了比较分析。结果表明:采用... 详细信息

采用共节点分离式钢筋混凝土模型,对框-筒结构折叠倒塌进行了三维数值模拟,包括底部起爆和顶部起爆2种方式,并且在底部起爆方式中,采用了不同切口高度和延迟时间。对结构的倒塌过程、支撑立柱的破坏过程进行了比较分析。结果表明:采用底部起爆时,结构下部压碎程度较采用顶部起爆严重;同为底部起爆,延迟时间0.25 s的结构破碎比0.5 s严重;底层立柱采用不同的切口高度和延迟时间,结构的倒塌方向发生了改变,结构略向南倾倒;支撑柱的破坏过程不同,对结构的后坐距离产生影响,采用底部起爆时,结构的后坐距离小于顶部起爆。通过对混凝土和钢筋单元受力过程进行的分析,共节点分离式模型能够反映钢筋和混凝土2种材料的力学性能差异。

关键词：爆炸力学折叠倒塌共节点分离式模型框-筒结构爆破拆除