Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

作     者：JIANG Lin-lin HAN Guang-ming LAN Yu LIU Sai-nan GAO Ji-ping YANG Xu MENG Jun CHEN Wen-fu 

作者机构：Liaoning Biochar Engineering & Technology Research Center Shenyang Agricultural University Shenyang 110866 P.R.China Rice Research Institute Shenyang Agricultural University Shenyang 110866 P.R.China Industrial Crops Institute Hubei Academy of Agricultural Sciences Wuhan 430064 P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2017年第16卷第3期

页      面：713-724页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 0713[理学-生态学] 

基　　金：funded by the National Natural Science Foundation of China (41401325 and 31501250) the Key Technologied R&D Program of China during the 12th Five-Year Plan period (2014BAD02B06-02) the Special Fund for Agro-scientific Research in the Public Interest, China (201303095) the Excellent Talent Support Program of Ministry of Liaoning Education, China 

主　　题：biochar soil microbial community DGGE Biolog Eco-plates 

摘      要：Biochar has been shown to influence soil microbial communities in terms of their abundance and ***,the relationship among microbial abundance,structure and C metabolic traits is not well studied under biochar *** it was hypothesized that the addition of biochar with intrinsic properties（i.e.,porous structure）could affect the proliferation of culturable microbes and the genetic structure of soil bacterial *** the meantime,the presence of available organic carbon in biochar may influence the C utilization capacities of microbial community in Biolog Eco-plates.A pot experiment was conducted with differenct biochar application（BC）rates：control（0 t ha^-1）,BC1（20 t ha^-1）and BC2（40 t ha^-1）.Culturable microorganisms were enumerated via the plate counting *** diversity was examined using denaturing gradient gel electrophoresis（DGGE）.Microbial capacity in using C sources was assessed using Biolog *** addition of biochar stimulated the growth of actinomyces and bacteria,especially the ammonifying bacteria and azotobacteria,but had no significant effect on fungi *** phylogenetic distribution of the operational taxonomic units could be divided into the following groups with the biochar addition：Firmicutes,Acidobacteria,Gemmatimonadetes,Actinobacteria,Cyanobacteria andα-,β-,γ-andδ-Proteobacteria（average similarity〉95%）.Biochar application had a higher capacity utilization for L-asparagine,Tween 80,D-mannitol,L-serine,γ-hydroxybutyric acid,N-acetyl-D-glucosamine,glycogen,itaconic acid,glycyl-L-glutamic acid,α-ketobutyricacid and putrescine,whereas it had received decreased capacities in using the other 20 carbon sources in Biolog *** analysis（RDA）revealed that the physico-chemical properties,indices of bacterial diversity,and C metabolic traits were positively correlated with the appearance of novel sequences under BC2 *** study indicates that the addition of biochar can incre