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Chinese Science Bulletin 2002年第24期47卷 2095-2099页

作者： jiang wei, zhao dehua,{2., tian jiesheng, wang zhenfang & li jilundepartment of microbiology, college of biological sciences, china agricultural university, beijing 100094, china Department of microbiology College of Biological Sciences China Agricultural University Beijing China

A submerged culture technique for Magneto-spirillum gryphiswaldense under the nitrogen-fixing condition (microaerobic and N-limited) was set up. In N-limited medium with Na-lactate as a sole carbon source, the optical density (A600 nm) and activity of nitrogen fixation of cells were 1.3 and 2.7 nmol of ethylene produced per hour per A600nm respectively within 2. h by three times of feeds. The pH and temperature were controlled at 7.2.and 30℃ respectively, and the oxygen concentration was controlled by sparging with N2.containing 0.4%-0.8% of O2. The activity of nitrogen fixation of cells was obviously inhibited by oxygen and ammonium. It indicated that the posttranslational regulation of nitrogenase existed in M. gryphiswaldense.

关键词： M. gryphiswaldense submerged culture nitrogen fixation regulation of nitrogenase activity.

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核糖核酸标准物质研制与应用进展

生物技术通报 2024年第6期40卷 105-113页

作者：王丹赵文江齐鑫李欣然李凯樊丽李亮内蒙古农业大学园艺与植物保护学院呼和浩特010019 黑龙江省农业科学院畜牧兽医分院齐齐哈尔161005 中国农业科学院农业质量标准与检测技术研究所北京100081

核糖核酸(RNA)标准物质是保证核酸体外诊断数据准确一致的重要实物标准,广泛用于病毒类疾病的检测、仪器校准、试剂盒评价及能力验证等工作中,其目的是保证测量过程和测量结果的准确一致。自1997年,WHO研制出了首个针对核酸扩增技术的... 详细信息

核糖核酸(RNA)标准物质是保证核酸体外诊断数据准确一致的重要实物标准,广泛用于病毒类疾病的检测、仪器校准、试剂盒评价及能力验证等工作中,其目的是保证测量过程和测量结果的准确一致。自1997年,WHO研制出了首个针对核酸扩增技术的丙型肝炎核酸国际标准物质,使全球不同实验室、不同方法检测的结果有了可比性。截至2.2.年,中国在国家标准物质资源共享平台发布的RNA有证标准物质有近百种,对于RNA标准物质的研究与应用,日益受到世界各国计量机构、相关国际学术组织与企业的重视,尤其是新型冠状病毒疫情暴发后,RNA标准物质成为了生物标准物质的研制热点。随着标准物质研制技术的不断发展,标准物质的量值准确性也有所提升,为测量结果的准确一致提供保障。通过对当前RNA标准物质的制备方法、分装、定值技术、不确定度评定及应用领域进行综述,为RNA标准物质的研制工作以及未来在临床医学、农业生产、动物医学、食品安全等领域的应用提供参考。

关键词：核糖核酸标准物质标准物质定值稳定性均匀性不确定度评定

Comparative study of estimation methods for genomic breeding values

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Science Bulletin 2016年第5期61卷 353-356页

作者： Chonglong wang Qin Zhang li {2. Rong Qian Xiangdong Ding Yaofeng {2. Institute of Animal Husbandry and Veterinary Medicine AnhuiAcademy of Agricultural Sciences Key Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture of China College of Animal Science and Technology China Agricultural University college of biological {2. China Agricultural University

Estimation of genomic breeding values is important in genomic selection. Bayesian and BLUP methods are the main techniques employed. In this study,we conducted a comparative study of Bayes A, Bayes B,Bayes Cp and GBLUP methods in simulated data and real data of Chinese Holstein cattle. Results showed that, in simulated data, the accuracies of all methods were all similarly elevated with the increase of reference population size, but they made different responses to the changes of marker number or QTL number. In real data of Chinese Holstein cattle, Bayes A generated the highest accuracy almost for all six traits, and GBLUP performed as well as Bayes A for the traits of milk yield, fat yield and protein yield, while for the trait of fat percentage, protein percentage and somatic cell score, three Bayesian methods showed superior to GBLUP. Comprehensively analyzing above results, it can be speculated that accuracies of the three Bayesian methods are not only influenced by the absolute value of QTL number or marker number, but may also be influenced by the ratio of QTL number to marker number. And there is at least one kind of Bayesian methods performing better than GBLUP, when the ratio of QTL number versus marker number is very small or involving large-effect QTL.

关键词： Genomic selection Estimation of genomic breeding values Bayesian methods BLUP methods

Effect of high temperature cooking on the quality of rice porridge

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International Journal of agricultural and biological Engineering 2021年第5期14卷 247-254页

作者： Cunshe Chen Siyuan jiang Mengfei li You li He li Fen zhao Zhihua Pang Xinqi liu beijing Engineering and Technology Research Center of Food Additives Beijing 100048China beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing 100048China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

In order to explore the effect of high-temperature cooking instant porridge on the taste and quality of porridge,this study explored the changes in the taste quality of rice porridge under different heating times(15 min,2. min,2. min and 30 min)at a higher initial temperature(100°C),and compared with that cooked at room temperature(2.°C),and explained the reasons for the *** the perspective of migration rate in moisture content,the rice porridge with special treatment has a more rapid moisture change,which makes texture,whiteness,smell and other factors change in a shorter time and increases the taste *** was concluded that under special treatment condition of starting with 100°C water and heating for 15 min,the kinds of rice flavor increased 2.2.times,the white of rice decreased by 10.04%,hardness decreased by 3.54 times,and the elasticity decreased by 35.48%*** general,cooking with higher initial temperature(100°C)is more valuable than cooking from 2.°C,in terms of the texture,flavor,saving time,and stable quality during the household and industrial production of instant porridge.

关键词： rice porridge low frequency NMR sensory evaluation GC-MS confocal laser scanning microscope

Modes of genetic adaptations underlying functional innovations in the rumen

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Science china(life {2.) 2021年第1期64卷 1-21页

作者： Xiangyu Pan Yudong Cai Zongjun li Xianqing Chen Rasmus Heller Nini wang Yu wang Chen {2. Yong wang Han Xu Songhai li Ming li Cunyuan li Shengwei Hu Hui li Kun wang Lei Chen Bin wei Zhuqing Zheng weiwei Fu Yue Yang Tingting Zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv wei Sun Xinyu li Shisheng Huang lixiang liu Shengyong Mao Wenqing liu Jinlian Hua Zhipeng li Guojie Zhang Yulin Chen Xihong wang Qiang Qiu Brian PDalrymple Wen wang Yu jiang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China School of Ecology and Environment Northwestern Polytechnical UniversityXi’an710072China Section for Computational and RNA Biology Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark university of Chinese Academy of sciences Beijing100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming650223China CEMS NCMISMDISAcademy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing100080China Marine Mammal and Marine Bioacoustics Laboratory Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanya572000China college of life {2. Shihezi UniversityShihezi832003China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Animal Science and TechnologyGuangxi UniversityNanning530005China college of Animal Science and Technology Yangzhou UniversityYangzhou225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of china Yangzhou UniversityYangzhou225009China Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510150China School of life Science and Technology Shanghai Tech UniversityShanghai201210China college of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China college of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering&TechnologyNorthwest A&F UniversityYangling712100China Department of Special Economic Animal Nutrition and Feed Science Institute of Special Animal and Plant SciencesChinese Academy of Agricultural SciencesChangchun130112China Section for Ecology and Evolution Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark china National GeneBank BGI-ShenzhenShen

The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant *** analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional *** identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other *** genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen *** modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory *** validated that the key ketogenesis rate-limiting gene(HMGCS2.with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other *** newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community ***,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene *** results greatly improve our understanding of rumen evolution and organ evo-devo in general.

关键词： rumen innovations comparative transcriptomics comparative genomics evo-devo evolution of organs

Exploring the stage-specific roles of Tcf-1 in T cell development and malignancy at single-cell resolution

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Cellular & Molecular Immunology 2021年第3期18卷 644-659页

作者： Fang wang Zhihong Qi yingpengao Guotao Yu Tao Feng {2.yan zhao Hai-Hui Xue Yaofeng zhao Peng jiang li Bao Shuyang Yu State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityYuanmingyuan West Road 2100193 BeijingChina Department of microbiology and Immunology Carver College of MedicineUniversity of IowaIowa CityIA 52242USA Regenerative Biology Laboratory Morgridge Institute for ResearchMadisonWI 53707USA Department Hematology Beijing Jishuitan Hospital100096 BeijingChina

Tcf-1(encoded by Tcf7)not only plays critical roles in promoting T cell development and differentiation but also has been identified as a tumor suppressor involved in preventing T cell ***,the comprehensive mechanisms of Tcf-1 involved in T cell transformation remain poorly *** this study,Tcf7^(fl/fl)mice were crossed with Vav-cre,Lck-cre,or Cd4-cre mice to delete Tcf-1 conditionally at the beginning of the HSC,DN2.DN3,or DP stage,*** defective T cell development phenotypes became gradually less severe as the deletion stage became more advanced in distinct mouse ***,consistent with Tcf7^(-/-)mice,Tcf^(fl/fl)Vav-cre mice developed aggressive T cell lymphoma within 45 weeks,but no tumors were generated in Tcf7^(fl/fl)Lck-cre or Tcf^(fl/fl)CdA-cre ***-cell RNA-seq(ScRNA-seq)indicated that ablation of Tcf-1 at distinct phases can subdivide DN1 cells into three clusters(C1,C2.and C3)and DN2.DN3 cells into three clusters(C4,C5,and C6).Moreover,Tcf-1 deficiency redirects bifurcation among divergent cell fates,and clusters C1 and C4 exhibit high potential for leukemic ***,we found that Tcf-1 directly binds and mediates chromatin accessibility for both typical T cell regulators and proto-oncogenes#including Myb,Mycn,Runxl,and Lyl1 in the DN1 phase and Lefb ld2.Dtxb Fyn,Bclllb,and Zfp36l2.in the DN2.DN3 *** aberrant expression of these genes due to Tcf-1 deficiency in very early T cells contributes to subsequent ***,we demonstrated that Tcf-1 plays stage-specific roles in regulating early thymocyte development and transformation,providing new insights and evidence for clinical trials on T-ALL leukemia.

关键词： ScRNA-seq Tcf-1 T cell Lymphoma development Malignancy Leukemic transformation

Abscisic Acid Receptor ABAR/CHLH Antagonizes Negative ABA Signaling Regulators ADSls to Relieve ABA-responsive Genes of Inhibition in Arabidopsis

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Abscisic Acid Receptor ABAR/CHLH Antagonizes Negative ABA Si...

The Interactions among DWARF10,Auxin and Cytokinin Underlie Lateral Bud Outgrowth in Rice

中国遗传学会植物遗传和基因组学专业委员会2.09年学术研讨会

作者： Yi Shang,~1 Lu Yan,~(1,2. Zhi-Qiang liu,~(1,2. Zheng Cao,~(1,2. Chao Mei,~1 Fu-Qing Wu,~(1,2. Qi Xin,~(1,2. Xiao-Fang wang,~(1,2. Shu-Yuan Du,~1 Tao {2.,~(1,2. Xiao-Feng Zhang,~1 Rui {2.,~(1,2. Hai-li Sun,~(1,2. Rui liu,~(1,2.,Yong-Tao Yu,~1 and Da-Peng Zhang~(1*) 1 Department of biological sciences and Biotechnology,Tsinghua university,beijing,100084,china. 2.State Key Laboratory of Plant Physiology and Biochemistry,china {12. university,beijing, 100094,

The phytohormone abscisic acid(ABA) plays a vital role in plant development and response to environmental challenges,but the ABA receptor-mediated signaling pathways have been poorly *** previously reported that a chl... 详细信息

关键词： Abscisic acid(ABA) ABA receptor ABAR/CHLH,ABA signaling.

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Journal of Integrative Plant Biology 2010年第7期52卷 626-638页

作者： Shuying Zhang Gang li Jun Fang {2.qi Chen Haipai jiang Junhuang Zou Xue liu Xianfeng {2. Xiaobing li Chengcai Chu Qi Xie Xiangning jiang lihuang Zhu The State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China college of biological {2. and Biotechnology Beijing Forestry University Beijing 100083 China college of Agronomy Shenyang Agricultural University Shenyang 110161 China Department of Ophthalmology University of Utah Salt Lake City UT 84132 USA Graduate university of Chinese Academy of sciences Beijing 100049 China

Previous studies have shown that DWARFIO （D10） is a rice ortholog of MAX41RMS1/DAD1, encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives （SL） biosynthesis. Here we use DIO. RNA interference （RNAi） transgenic plants similar to dlO mutant in phenotypes to investigate the interactions among DIO, auxin and cytokinin in regulating rice shoot branching. Auxin levels in node 1 of both decapitated *** and wild type plants decreased significantly, showing that decapitation does reduce endogenous auxin concentration, but decapitation has no clear effects on auxin levels in node 2.of the same plants. This implies that node 1 may be the location where a possible interaction between auxin and DIO gene would be detected. DIO expression in node 1 is inhibited by decapitation, and this inhibition can be restored by exogenous auxin application, indicating that DIO may play an important role in auxin regulation of SL. The decreased expression of most OsPINs in shoot nodes of DIO-RNAi plants may cause a reduced auxin transport capacity. Furthermore, effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that DIO promotes cytokinin biosynthesis by reducing auxin levels. Besides, in DIO-RNAi plants, decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents, resulting in more tillering phenotypes.

关键词： AUXIN PLANT hormones CYTOKININS RICE PHENOTYPE

Inhibition of the maize salt overly sensitive pathway by ZmSK3 and ZmSK4

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Journal of Genetics and Genomics 2023年第12期50卷 960-970页

作者： Jianfang li Xueyan Zhou Yan wang Shu Song liang Ma Qian He Minhui Lu Kaina Zhang Yongqing Yang Qian zhao weiwei Jin Caifu {2. Yan Guo State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China college of life {2. Qingdao Agricultural UniversityQingdaoShandong 266109China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100093China National Maize Improvement Center of china China Agricultural UniversityBeijing 100193China

Soil salinity is a worldwide problem that adversely affects plant growth and crop productivity. The salt overly sensitive (SOS) pathway is evolutionarily conserved and essential for plant salt tolerance. In this study, we reveal how the maize shaggy/glycogen synthase kinase 3-like kinases ZmSK3 and ZmSK4, orthologs of brassinosteroid insensitive 2.in Arabidopsis thaliana, regulate the maize SOS pathway. ZmSK3 and ZmSK4 interact with and phosphorylate ZmSOS2. a core member of the maize SOS pathway. The mutants defective in ZmSK3 or ZmSK4 are hyposensitive to salt stress, with higher salt-induced activity of ZmSOS2.than that in the wild type. Furthermore, the Ca^(2.) sensors ZmSOS3 and ZmSOS3-like calcium binding protein 8 (ZmSCaBP8) activate ZmSOS2.to maintain Na^(+)/K^(+) homeostasis under salt stress and may participate in the regulation of ZmSOS2.by ZmSK3 and ZmSK4. These findings discover the regulation of the maize SOS pathway and provide important gene targets for breeding salt-tolerant maize.

关键词： Salt stress Maize SOS pathway ZmSK3 ZmSK4 ZmSCaBP8

昆虫病原线虫共生菌及代谢产物对根结线虫的防治作用

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土壤与作物 2024年第3期13卷 381-389页

作者：吴黎黎谢倚帆常豆豆黄铭慧姜野秦瑞峰蒋丹韦柳利王旋赵亚男窦悦文王从丽李春杰中国科学院东北地理与农业生态研究所大豆分子设计育种院重点实验室黑龙江哈尔滨150081 中国科学院大学北京100049 铁力市农业农村局黑龙江铁力152500 齐齐哈尔市农业技术推广中心黑龙江齐齐哈尔161006

为探讨不同种昆虫病原线虫(EPN)共生菌对根结线虫病的防治效果,开展了温室盆栽定量接种试验;为明确EPN共生菌的代谢产物对根结线虫卵孵化和二龄幼虫活性的影响,进行了室内生测试验。结果表明,Meloidogyne incognita(***)在中蔬4号番茄... 详细信息

为探讨不同种昆虫病原线虫(EPN)共生菌对根结线虫病的防治效果,开展了温室盆栽定量接种试验;为明确EPN共生菌的代谢产物对根结线虫卵孵化和二龄幼虫活性的影响,进行了室内生测试验。结果表明,Meloidogyne incognita(***)在中蔬4号番茄上接种剂量为1000、2.00和5000个卵·株^(−1)时,昆虫病原线虫共生菌Xb-IGA和Pl-IGA对***卵的繁殖均有较好的抑制效果,抑制效果为59.4%~73.9%。Meloidogyne hapla(***)在VFNT番茄上接种剂量为2.00个卵·株^(−1)时,昆虫病原线虫共生菌Pl-IGA对***卵的繁殖抑制效果最好,可达74.5%,与常规药剂阿维菌素(82.2.)差异不显著。4种EPN共生菌均能有效降低***和***侵染后的发病程度,对番茄根部生长有不同程度的促进作用。在生测试验7 d时,4种昆虫病原线虫共生菌代谢产物的2.稀释液和5倍稀释液对***和***卵孵化抑制率为55.1%~94.8%,对***二龄幼虫校正死亡率为34.2.~47.8%,对***二龄幼虫校正死亡率为59.6%~79.9%。因此,昆虫病原线虫共生菌及其代谢产物对根结线虫是非常有潜力的生物杀线虫资源。

关键词：根结线虫昆虫病原线虫共生菌代谢产物防效