The Interactions among DWARF10,Auxin and Cytokinin Underlie Lateral Bud Outgrowth in Rice

作     者：Shuying Zhang Gang Li Jun Fang Weiqi Chen Haipai Jiang Junhuang Zou Xue Liu Xianfeng Zhao Xiaobing Li Chengcai Chu Qi Xie Xiangning Jiang Lihuang Zhu 

作者机构：The State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China College of Agronomy Shenyang Agricultural University Shenyang 110161 China Department of Ophthalmology University of Utah Salt Lake City UT 84132 USA Graduate University of Chinese Academy of Sciences Beijing 100049 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2010年第52卷第7期

页      面：626-638页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 0710[理学-生物学] 071001[理学-植物学] 07[理学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (30623011) 

主　　题：AUXIN PLANT hormones CYTOKININS RICE PHENOTYPE 

摘      要：Previous studies have shown that DWARFIO （D10） is a rice ortholog of MAX41RMS1/DAD1, encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives （SL） biosynthesis. Here we use DIO. RNA interference （RNAi） transgenic plants similar to dlO mutant in phenotypes to investigate the interactions among DIO, auxin and cytokinin in regulating rice shoot branching. Auxin levels in node 1 of both decapitated *** and wild type plants decreased significantly, showing that decapitation does reduce endogenous auxin concentration, but decapitation has no clear effects on auxin levels in node 2 of the same plants. This implies that node 1 may be the location where a possible interaction between auxin and DIO gene would be detected. DIO expression in node 1 is inhibited by decapitation, and this inhibition can be restored by exogenous auxin application, indicating that DIO may play an important role in auxin regulation of SL. The decreased expression of most OsPINs in shoot nodes of DIO-RNAi plants may cause a reduced auxin transport capacity. Furthermore, effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that DIO promotes cytokinin biosynthesis by reducing auxin levels. Besides, in DIO-RNAi plants, decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents, resulting in more tillering phenotypes.