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The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line

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Acta Pharmaceutica Sinica B 2015年第6期5卷 554-563页

作者： Jun Xie jiahui liu Heng liu Shihui Liang Meigui Lin Yueyu Gu Taoli liu Dongmei Wang Hui Gee Sui-lin Mo Department of Traditional Chinese Medicine The First Affiliated HospitalSun Yat-sen University Huanghuagang Street Community Health Service Center Liwan District Shiweitang Street Community Health Service Center School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou Higher Education Mega Center Health Care and Physical Examination Center The First Affiliated HospitalSun Yat-sen University

The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGFNEGFR signal pathway were investigated. The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs. The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA (2.5-80 mu mol/E) for 24, 48 and 72 h, respectively. Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation. VEGF and VEGFR2 expression were studied by Western blotting. The binding mode of tanshinone IIA within the crystal stmcture of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1. The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose- and time-dependent manner. Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control, and tanshinone IIA-treated cells accumulated at the S phase, which was higher than the vehicle control. Furthermore, the expression of VEGF and VEGFR2 was decreased in Western blot Finally, molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and pi-pi stacking interactions with Va1848. In conclusion, tanshinone IIA may suppress A549 proliferation, induce apoptosis and cell cycle arrest at the S phase. This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Non-small cell lung cancer Tanshinone IIA VEG/VEGFR signal pathway Molecular docking

基于机电液联合仿真的高空测试转台俯仰运动控制

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机械制造与自动化 2024年第3期53卷 215-218页

作者：丁彦苏邓文翔刘家辉姚建勇南京理工大学机械工程学院江苏南京210094

针对高空测试转台俯仰运动存在负载不平衡以及液压缸之间的耦合效应,通过Adams和AMESim分别构建高空测试转台三维俯仰转台的刚柔耦合动力学模型和电液控制系统模型,采用PID控制算法对测试转台俯仰系统技术指标要求的工况进行联合仿真。... 详细信息

针对高空测试转台俯仰运动存在负载不平衡以及液压缸之间的耦合效应,通过Adams和AMESim分别构建高空测试转台三维俯仰转台的刚柔耦合动力学模型和电液控制系统模型,采用PID控制算法对测试转台俯仰系统技术指标要求的工况进行联合仿真。结果表明:俯仰平台的实际角度能很好地跟踪角度指令,满足定向精度要求,为后续的试验研究奠定了理论基础。该方法弥补了单一仿真软件不能对系统进行完整仿真的不足,可以更加全面、准确地反映研究对象的真实控制与运动状态,对于复杂的机电液一体化系统具有普遍的适用性。

关键词：高空测试转台刚柔耦合 PID控制联合仿真

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新生儿PICC相关性血栓预防与管理的证据总结

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中国护理管理 2024年第3期24卷 418-424页

作者：方运霞刘佳惠林琴彭思意魏涛袁忠王童语李旭英湖南中医药大学护理学院长沙市410208 湖南省肿瘤医院乳腺内科湖南省肿瘤医院血管通道中心湖南省肿瘤医院早期临床中心湖南省肿瘤医院麻醉科湖南省肿瘤医院护理部

目的:总结新生儿经外周静脉置入中心静脉导管(PICC)相关性血栓预防与管理的证据,为临床医护人员提供循证依据。方法:系统检索国内外临床决策网站、指南网站、专业协会网站、循证文献资源库及原始文献数据库,检索时限为建库至2023年2月... 详细信息

目的:总结新生儿经外周静脉置入中心静脉导管(PICC)相关性血栓预防与管理的证据,为临床医护人员提供循证依据。方法:系统检索国内外临床决策网站、指南网站、专业协会网站、循证文献资源库及原始文献数据库,检索时限为建库至2023年2月。对纳入的文献进行方法学质量评价,结合专业判断、临床情境提取证据并进行汇总。结果:共纳入14篇文献,其中临床决策2篇,指南6篇,系统评价4篇,队列研究2篇。从风险评估、导管置入、导管维护、血栓诊断、血栓治疗、拔管等6个方面共汇总了31条证据。结论:本研究总结了新生儿PICC相关性血栓预防及管理的证据。医护人员可结合文化背景、临床情境选择证据,制定科学的血栓预防及管理方案,提升护理质量。

关键词：导管相关性血栓经外周静脉置入中心静脉导管新生儿证据总结

Visible-light-induced photocatalytic CO_(2)reduction over zirconium metal organic frameworks modified with different functional groups

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Journal of Environmental Sciences 2023年第10期132卷 22-30页

作者： Yuexian Du Guang’an Jie Huilin Jia jiahui liu Jieyu Wu Yanghe Fu Fumin Zhang Weidong Zhu Maohong Fan Key Laboratory of the Ministry of Education for Advanced Catalysis Materials Institute of Physical ChemistryZhejiang Normal UniversityJinhua 321004China Zhejiang Engineering Laboratory for Green Syntheses and Applications of Fluorine-Containing Specialty Chemicals Institute of Advanced Fluorine-Containing MaterialsZhejiang Normal UniversityJinhua 321004China Department of Chemical and Petroleum Engineering University of WyomingLaramieWY 82071USA

The reduction of CO_(2)into high value-added chemicals and fuels by a photocatalytic technology can relieve energy shortages and the environmental problems caused by greenhouse *** the current work,an amino-functionalized zirconium metal organic framework(Zr-MOF)was covalently modified with different functional groups via the condensation of Zr-MOF with 2-pyridinecarboxaldehyde(PA),salicylaldehyde(SA),benzaldehyde(BA),and trifluoroacetic acid(TA),named Zr-MOF-X(X=PA,SA,BA,and TA),respectively,through the post-synthesis *** with Zr-MOF and Zr-MOF-TA,the introduction of PA,SA,or BA into the framework of Zr-MOF can not only enhance the visible-light harvesting and CO_(2)capture,but also accelerate the photogenerated charge separation and transfer,thereby improving the photocatalytic ability of Zr-MOF for CO_(2)*** results indicate that the modification of Zr-MOF with electron-donating groups can promote the photocatalytic CO_(2)***,the current work provides an instructive approach to improve the photocatalytic efficiency of CO_(2)reduction through the covalent modification of MOFs.

关键词： Photocatalysis CO_(2)reduction Metal organic frameworks Post-synthesis modification Electric effect

一体化^(18)F-FET PET/MR术前评估成人胶质瘤患者MGMT基因启动子甲基化状态

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磁共振成像 2024年第7期15卷 21-26页

作者：韩青青李拓林增萍李恩慧杨阳刘家惠邢浩王裕程欣霍力中国医学科学院北京协和医学院北京协和医院核医学科北京100730 上海联影医疗高新技术研究院有限公司上海201807 北京联影智能影像技术研究院北京100094 中国医学科学院北京协和医学院北京协和医院神经外科北京100730 核医学分子靶向诊疗北京市重点实验室北京100730

目的旨在研究一体化^(18)F-氟乙基酪氨酸(^(18)F-fluoroethyltyrosine, ^(18)F-FET)正电子发射断层成像(positron emission tomography, PET)/MR对成人胶质瘤的O6-甲基鸟嘌呤DNA甲基转移酶(O6-methylguanine DNA methyltransferase, MGMT)基因启动子甲基化状态的鉴别能力。材料与方法回顾性分析未经活检或治疗的胶质瘤患者资料16例,均完成一体化PET/MR扫描,包括^(18)F-FET PET、常规MRI及体素内不相干运动(intravoxel incoherent motion, IVIM)成像。以靶本比(target-background ratio, TBR)=1.6为阈值对PET图像进行感兴趣体积(volume of interest, VOI)分割,通过刚性配准获得肿瘤VOI对应的IVIM图及其参数表观扩散系数(apparent diffusion coefficient, ADC)、真实扩散系数(true diffusion coefficient, D)、伪扩散系数(pseudo diffusion coefficient, D^(*))、灌注分数(perfusion fraction, f)、分布扩散系数(distributed diffusion coefficient, DDC)和异质性指数(heterogeneity index, α),使用pyradiomics对各参数进行特征提取,获得对应的一阶灰度直方图特征。计算每个IVIM参数图对应的特征值与^(18)F-FET PET参数的关系,并利用组间比较和受试者工作特征(receive operating characteristic, ROC)曲线分析探究PET和IVIM参数对MGMT启动子甲基化的区分能力。结果 IVIM-α的两个特征值90分位值(r=0.526,P<0.05)和最大值(r=0.520, P<0.05)与PET参数平均标准摄取值(meanstandarduptakevalue,SUVmean)呈正相关;IVIM-α和SUVmean在MGMT启动子甲基化状态阳性和阴性两组之间的差异有统计学意义(P<0.05),阳性组的IVIM-α均值和SUVmean显著高于阴性组;融合IVIM-α均值和SUVmean对MGMT启动子甲基化状态进行区分的曲线下面积(area under the curve, AUC)为0.77。结论基于一体化PET/MR的^(18)F-FET PET和IVIM参数能够有效预测胶质瘤的MGMT启动子甲基化状态。

关键词：胶质瘤 O6-甲基鸟嘌呤-DNA甲基转移酶启动子甲基化 ^(18)F-氟乙基酪氨酸正电子发射断层成像正电子发射断层成像/磁共振体素内不相干运动磁共振成像

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某铰接式铁水罐车动力学性能分析

机车电传动 2024年第3期 79-89页

作者：田明洁黄志辉杨权于宏达刘加蕙西南交通大学轨道交通运载系统全国重点实验室四川成都610031 成都西部物联集团有限公司四川攀枝花617000

文章基于多体动力学软件Simpack建立了某铰接式铁水罐车的动力学模型,从理论和仿真两方面计算了不同铰接位置时的轴重分配情况;对比分析了不同铰接位置铁水罐车曲线通过性能,结合轴重分配确定了最佳铰接位置,并校核了最佳铰接位置时铁... 详细信息

文章基于多体动力学软件Simpack建立了某铰接式铁水罐车的动力学模型,从理论和仿真两方面计算了不同铰接位置时的轴重分配情况;对比分析了不同铰接位置铁水罐车曲线通过性能,结合轴重分配确定了最佳铰接位置,并校核了最佳铰接位置时铁水罐车的非线性临界速度;最后研究了轨道不平顺、曲线半径等线路参数及运行速度对铁水罐车小半径曲线运行安全性的影响。结果表明,最佳铰接位置是车辆枕簧中心与一位轮对中心的纵向距离为其与中间轴箱后臂铰接点纵向距离50%的位置,此时铁水罐车各轴轴重分配均衡,曲线通过性能最好;铁水罐车非线性临界速度为40km/h,满足运行要求且有一定的安全裕量;轨道不平顺对铁水罐车小半径曲线运行安全性能指标有显著影响,曲线半径次之,曲线超高影响不大,并在不同的轨道不平顺和曲线半径下选取了较安全的运行速度。

关键词：铁水罐车动力学性能铰接式三轴转向架轴重均衡曲线通过安全性运行参数

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肿瘤专科医院新入职护士培训目标清单的构建

中华护理教育 2024年第2期21卷 152-158页

作者：刘佳惠李金花李旭英陈婕君李星凤王婧胡美华湖南省肿瘤医院乳腺内科长沙市410031 湖南省肿瘤医院护理部长沙市410031

目的构建肿瘤专科医院新入职护士培训目标清单。方法基于文献回顾法、教学计划开发法、专家咨询法构建肿瘤专科医院新入职护士培训目标清单,采用四象限定位法对培训目标进行定位。结果最终构建的肿瘤专科医院新入职护士培训目标清单... 详细信息

目的构建肿瘤专科医院新入职护士培训目标清单。方法基于文献回顾法、教学计划开发法、专家咨询法构建肿瘤专科医院新入职护士培训目标清单,采用四象限定位法对培训目标进行定位。结果最终构建的肿瘤专科医院新入职护士培训目标清单包括6项一级指标(基础知识与技能、制度与标准、肿瘤专科知识与技能、职业素养、安全管理、护理科研与教学)和42项二级指标。四象限定位结果显示,处于“重要易培育”象限的培训目标有7项,处于“重要难培育”象限的培训目标有19项,处于“次重要易培育”象限的培训目标有12项,处于“次重要难培育”象限的培训目标有4项。结论构建的肿瘤专科医院新入职护士培训目标清单内容具有科学性,符合肿瘤专科医院新入职护士培训的需求,可为肿瘤专科医院开展新入职护士培训工作提供参考。

关键词：肿瘤专科医院新入职护士培训

A pan-cancer analysis of the biological function and clinical value of BTLA in tumors

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BIOCELL 2023年第2期47卷 351-366页

作者： XIANGLAI JIANG JIN HE YONGFENG WANG jiahui liu XIANGYANG LI XIANGUI HE HUI CAI Graduate School Ningxia Medical UniversityYinchuan750004China General Surgery Clinical Medical Center Gansu Provincial HospitalLanzhou730000China Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province Gansu Provincial HospitalLanzhou730000China NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor Gansu Provincial HospitalLanzhou730000China The First Clinical Medical College of Lanzhou University Lanzhou730000China

B and T-lymphocyte attenuator(BTLA)plays an immunosuppressive role by inhibiting T-and B-cell *** is associated with a variety of diseases,especially cancer ***,the function of BTLA in various cancers and its clinical prognostic value have still not been comprehensively *** study aimed to identify the relationship between BTLA and cancer from the perspectives of differences in BTLA expression,its clinical value,immune infiltration,and the correlation with immune-related genes in various *** regarding mRNA expression,miRNA expression,lncRNA expression,and clinical data of patients of 33 existing cancers were collected from the TCGA *** miRNA of BTLA and the lncRNA that interacts with the target miRNA were obtained from the StarBase *** on bioinformatics analysis methods,the relationship between various types of cancers and BTLA was thoroughly investigated,and a competing endogenous RNA network of BTLA,target miRNA,and interacting lncRNA was *** Kaplan-Meier(KM)prognostic analysis of BTLA and target miRNA(has-miR-137)in various types of cancers was completed using the KM *** expression varied in different cancers,with statistical significance in nine cancer *** plotter to analyze the overall survival(OS)and regression-free survival prognosis of cancer patients revealed that the BTLA expression was statistically different in the OS of 11 types of cancers out of 21 types of cancers;the OS of 8 type of cancers was also statistically *** analysis of tumor immune genes revealed a positive correlation of BTLA expression with immunosuppressive gene(CTLA4 and PDCD1)*** Set Enrichment Analysis showed that BTLA and its co-expressed genes mainly act through biological processes and pathways,including immune response regulation,cell surface receptor signaling pathway,antigen binding,antigen receptor-mediated signaling pathway,and leukocyte *** has t

关键词： Pan-cancer BTLA Tumor immunity Clinical value

急性心肌梗死患者血浆前蛋白转化酶枯草溶菌素9水平的影响因素研究

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中国全科医学 2024年第36期27卷 4568-4574页

作者：贾佳刘嘉慧季文君郑博王新刚范芳芳李寅张龙张岩北京大学第一医院心血管内科北京市100034 北京大学第一医院医学统计室北京市100034 北京大学第一医院心血管疾病研究所北京市100034

背景前蛋白转化酶枯草溶菌素9(PCSK9)对脂质代谢起到重要调节作用,急性心肌梗死患者血浆PCSK9水平的影响因素尚未完全阐明。目的了解急性心肌梗死患者血浆PCSK9水平的影响因素。方法连续纳入2010—2018年于北京大学第一医院心血管内科... 详细信息

背景前蛋白转化酶枯草溶菌素9(PCSK9)对脂质代谢起到重要调节作用,急性心肌梗死患者血浆PCSK9水平的影响因素尚未完全阐明。目的了解急性心肌梗死患者血浆PCSK9水平的影响因素。方法连续纳入2010—2018年于北京大学第一医院心血管内科入院诊断为急性心肌梗死的患者。基线信息采集患者入院时电子病历系统中的现病史、既往史、体格检查相关信息,检测基线血浆PCSK9水平。影响因素分析采用单因素及多因素线性回归模型,通过LASSO法进行筛选并纳入多因素线性回归模型。结果共入选996例急性心肌梗死患者,其中37例患者因留存血样容量不足无PCSK9检测结果,共959例患者纳入分析,PCSK9水平为543.1(425.4,692.1)ng/mL。多因素回归分析结果显示,性别是影响急性心肌梗死患者PCSK9水平最大的因素(标准化回归系数为0.13),其余对PCSK9水平影响较大的因素依次为:心房颤动病史(标准化回归系数为0.09)、白细胞计数(标准化回归系数为0.07)及血尿酸水平(标准化回归系数为0.07)。结论在急性心肌梗死患者中,性别、心房颤动病史、白细胞计数及血尿酸水平与血浆PCSK9水平显著独立相关。

关键词：前蛋白转化酶枯草溶菌素9 急性心肌梗死胆固醇冠心病影响因素分析

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