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Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus

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Biomedical and Environmental Sciences 2011年第4期24卷 431-437页

作者： CAI Liang TAO XiaoYan liu YunZhi ZHANG Hong GAO LiDong HU ShiXiong liu FuQiang LI Hao SHEN XinXin liu jiahui WANG ShiQing TANG Qing Hunan Center for Disease Control and Prevention Key Laboratory of Microbial Molecular Biology of Hunan Province Changsha 410005 Hunan China State Key Laboratory for Molecular Virology and Genetic Engineering Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China

Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N *** Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific *** amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into *** XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive ***,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N *** The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% *** evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic *** The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.

关键词： Rabies N gene Molecular Characteristics Phylogenetic

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斑马鱼遗传操作进展

生物工程学报 2017年第10期33卷 1674-1692页

作者：高煜刘家辉王新刘东南通大学教育部江苏省神经再生重点实验室江苏南通226001

斑马鱼已经成为在发育生物学与疾病模型研究中应用最广泛的实验动物之一,其中多种遗传操作方法和高分辨率活体成像技术对斑马鱼的应用起了重要推动作用。斑马鱼在功能基因组学研究上有许多优势,如可以快速便捷地鉴定脊椎动物新基因的功... 详细信息

斑马鱼已经成为在发育生物学与疾病模型研究中应用最广泛的实验动物之一,其中多种遗传操作方法和高分辨率活体成像技术对斑马鱼的应用起了重要推动作用。斑马鱼在功能基因组学研究上有许多优势,如可以快速便捷地鉴定脊椎动物新基因的功能。本文尝试以示意图来简要总结应用于斑马鱼的遗传操作方法和基本原理,包括ENU化学诱变筛选的基本原理和隐性突变3代筛选的流程,逆转录病毒介导的插入突变的原理及其隐性突变3代筛选的流程,转座子Sleeping Beauty(SB)和Tol2介导的基因捕获载体系统(Gene-breaking transposon,GBT)为基础的基因捕获突变的基本原理,Morpholino介导的基因下调和micro RNA下调技术,TILLING(Targeting induced local lesions in genomes)技术和CEL1分析的基本原理和实验流程,锌指核酸酶ZFN(Zinc finger nuclease)与TALEN(Transcription activator-like effector nuclease)、CRISPR/Cas9定向基因失活的基本原理,斑马鱼上广泛采用的转基因技术的几种类型等。

关键词：斑马鱼遗传操作突变转基因转座子基因编辑

Probability Density Evolution Method for Vehicle Dynamics Analysis Under Uncertainty

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Probability Density Evolution Method for Vehicle Dynamics An...

Life Cycle Assessment and Prediction of Proton Exchange Membrane Fuel Cell Vehicles for 2020

2018中国汽车工程学会年会

作者： CHEN Xiaokai LEI Hao ZHANG Cheng liu jiahui Beijing Institute of Technology

Because of the uncertainties and highly nonlinear characteristics of intelligent vehicles,how to control the motion of intelligent vehicles more accurately and effectively has become the key to the development of intelligent *** this paper,based on the nonlinearity and parametric uncertainty of the suspension system,the probability density evolution method is used to analyse the variation of the probability density of the suspension dynamic response over time,in order to facilitate more effective motion *** the dynamic random state equation of the suspension and the probability conservation principle in the conservative stochastic system,the generalized probability density evolution equation for vehicle random vibration is *** the nonlinear stiffness of the hydro-pneumatic spring in the suspension system and the uncertainties of the parameters,such as the suspension and the tire,the stochastic vibration response analysis was performed with the sprung-mass acceleration response as an *** random probability space is discretely selected by the Number Theoretic ***-difference method such as Lax-Wendroff scheme and TVD（Total Variation Diminishing） scheme is used to numerically solve the generalized probability density evolution equation,and the variation of the probability density of the response is *** probability density evolution method has good calculation accuracy and can give specific probability distribution information,which lays a foundation for subsequent motion control.

关键词： suspension nonlinearity uncertainty probability density evolution method

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Life Cycle Assessment and Prediction of Proton Exchange Memb...

2018中国汽车工程学会年会

作者： CHEN Yisong DING Zhensen liu jiahui MA Jinqiu School of Automobile Chang’an University

Currently,fuel cell vehicles have attracted the attention of China and even the world,which perhaps will become the commanding point of technical competition in the automotive *** modeling with GaBi software,the prediction and quantitative analysis are made on life cycle energy saving and emission reduction for fuel cell vehicles in China in 2020 based on the 2020 target of China and the *** of Energy 2020 ***,sensitivity analysis was conducted for selected key *** result shows that the total resource depletion,energy consumption and environmental impact for fuel cell vehicles of China in its complete life cycle were 0.354 kg Sb-Eq.,7.38 × 105 MJ and 4.48 × 104 kg CO *** 2020,the impact of the power generation on the environment will decrease as the proportion of thermal power generation in the power structure declines,and it will provide a possibility for largescale electrolysis of water to produce hydrogen.

关键词： fuel cell vehicles life cycle assessment DOE 2020 target energy depletion and environmental impact

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甘肃陇南不同种类花椒籽的营养成分对比研究

中国饲料 2024年第14期 14-19页

作者：张莉古雪艳韩富军徐国伟张康王磊郭志廷李建喜 Hanyurwumutima Egide 刘佳惠张洺溪陈海瑜张景艳王学智甘肃省动物疫病预防控制中心甘肃兰州730046 中国农业科学院兰州畜牧与兽药研究所甘肃兰州730050 甘肃省农业科学院林果花卉研究所甘肃兰州730070 中国农业科学院兰州兽医研究所甘肃兰州730030

文章旨在以甘肃省陇南市文县九月青花椒籽、红花椒籽、无刺嫁接红花椒籽、为研究对象,采用凯氏定氮法、色谱法等测定花椒籽中蛋白、挥发油等主要营养成分的含量,评价3个不同种类花椒籽的饲用价值。结果表明,经过品种改良后,无刺嫁接花... 详细信息

文章旨在以甘肃省陇南市文县九月青花椒籽、红花椒籽、无刺嫁接红花椒籽、为研究对象,采用凯氏定氮法、色谱法等测定花椒籽中蛋白、挥发油等主要营养成分的含量,评价3个不同种类花椒籽的饲用价值。结果表明,经过品种改良后,无刺嫁接花椒籽中的水分、总灰分和粗纤维含量下降,粗蛋白和粗脂肪含量增加;无刺嫁接红花椒籽中粗脂肪、粗蛋白和挥发油含量显著高于九月青花椒籽和红花椒籽(P<0.05)。无刺嫁接红花椒籽中α-亚油酸、棕榈酸、硬脂酸、顺油酸、顺亚油酸和十五烷酸中含量显著高于九月青花椒籽(P<0.05)。无刺嫁接红花椒籽中的天冬氨酸、谷氨酸、精氨酸、甘氨酸、亮氨酸、丙氨酸、丝氨酸、苯丙氨酸、苏氨酸、缬氨酸、异亮氨酸、脯氨酸、组氨酸含量和氨基酸总量均显著高于九月青花椒籽和红花椒籽中的氨基酸含量(P<0.05)。无刺嫁接红花椒籽所含必需氨基酸中异亮氨酸、赖氨酸、蛋氨酸、苏氨酸均低于FAO/WHO的推荐值,且只在无刺嫁接红花椒籽中检测出蛋氨酸,其中亮氨酸、苯丙氨酸+酪氨酸和缬氨酸均高于FAO/WHO的推荐值。综上所述,3个不同种类的花椒籽中各组成成分种类和含量有所不同,其中无刺嫁接花椒籽富含多种营养成分,具有良好的饲用开发价值。

关键词：花椒籽不同品种甘肃陇南营养价值

Fibroblast-localized lncRNA CFIRL promotes cardiac fibrosis and dysfunction in dilated cardiomyopathy

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Science China(Life Sciences) 2024年第6期67卷 1155-1169页

作者： Shuai Yuan Xudong Zhang Jiabing Zhan Rong Xie jiahui Fan Beibei Dai Yanru Zhao Zhongwei Yin Qian liu Dao Wen Wang Huaping Li Chen Chen Division of Cardiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders Wuhan 430030China Department of Forensic Medicine Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

CFIRL is a long noncoding RNA(lnc RNA),we previously identified as the most significantly upregulated lnc RNA in the failing hearts of patients with dilated cardiomyopathy(DCM).In this study,we determined the function of CFIRL and its role in ***-time polymerase chain reaction and in situ hybridization assays revealed that CFIRL was primarily localized in the nucleus of cardiac fibroblasts and robustly increased in failing *** knockdown or fibroblast-specific knockout of CFIRL attenuated transverse aortic constriction(TAC)-induced cardiac dysfunction and fibrosis in *** of CFIRL in vitro promoted fibroblast proliferation and aggravated angiotensin II-induced differentiation to *** knockdown attenuated these ***,RNA pull-down assay and gene expression profiling revealed that CFIRL recruited ENO1,a newly identified noncanonical transcriptional factor,to activate ***-6 exerted a paracrine effect on cardiomyocytes to promote cardiac hypertrophy,which can be prevented by CFIRL *** findings uncover the critical role of CFIRL,a fibroblast-associated lnc RNA,in heart failure by facilitating crosstalk between fibroblasts and *** knockdown might be a potent strategy to prevent cardiac remodeling in heart failure,particularly in DCM.

关键词： lncRNA fibroblast fibrosis dilated cardiomyopathy heart failure

Enhancing the crystallinity and stability of perovskite solar cells with 4-tert-butylpyridine induction for efficiency exceeding 24%

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Journal of Energy Chemistry 2024年第6期93卷 1-7,I0001页

作者： You liu Lishuang Zheng Kuanxiang Zhang Kun Xu Weicheng Xie Jue Zhang Yulu Tian Tianyuan liu Hanzhong Xu Ruoming Ma Wei Huang jiahui Chen Jusheng Bao Chen Chen Yongsheng Zhou Xuchun Wang Junming Chen Jungan Wang College of Chemistry and Materials Engineering Anhui Science and Technology UniversityBengbu 233000AnhuiChina Anhui Province Quartz Sand Purification and Photovoltaic Glass Engineering Research Center Chuzhou 233100AnhuiChina Triumph Photovoltaic Materials Co.Ltd BengbuAnhuiChina National Laboratory of Solid State Microstructures School of PhysicsNanjing UniversityNanjing 210009JiangsuChina JinkoSolar Holding Co.Ltd HainingZhejiangChina

Perovskite solar cells(PSCs)have emerged as a promising photovoltaic technology because of their high light absorption coefficient,long carrier diffusion distance,and tunable ***,PSCs face challenges such as hysteresis effects and stability *** this study,we introduced a novel approach to improve film crystallization by leveraging 4-tert-butylpyridine(TBP)molecules,thereby enhancing the performance and stability of *** findings demonstrate the effective removal of PbI_(2)from the perovskite surface through strong coordination with TBP ***,by carefully adjusting the concentration of the TBP solution,we achieved enhanced film crystallinity without disrupting the perovskite *** TBP-treated perovskite films exhibit a low defect density,improved crystallinity,and improved carrier *** a result,the PSCs manufactured with TBP treatment achieve power conversion efficiency(PCE)exceeding 24%.Moreover,we obtained the PCE of 21.39%for the 12.25 cm^(2)module.

关键词： 4-tert-butylpyridine Film crystallization Perovskite solar cells Power conversion efficiency Stability improvement

Neural stem cell-derived exosomes promote mitochondrial biogenesis and restore abnormal protein distribution in a mouse model of Alzheimer's disease

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Neural Regeneration Research 2024年第7期19卷 1593-1601页

作者： Bo Li Yujie Chen Yan Zhou Xuanran Feng Guojun Gu Shuang Han Nianhao Cheng Yawen Sun Yiming Zhang jiahui Cheng Qi Zhang Wei Zhang Jianhui liu Department of Radiology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Morphology and Spatial Multi-Omics Technology Platform Shanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghaiChina Department of Anesthesiology Tongji HospitalSchool of MedicineTongji UniversityShanghaiChina Department of Blood Transfusion Huashan HospitalFudan UniversityShanghaiChina

Mitochondrial dysfunction is a hallmark of Alzheimer’s *** previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 *** Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a ***,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial ***,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse *** findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-β***,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial *** addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.

关键词： Alzheimer’s disease mitochondrial biogenesis neural stem cell-derived exosome SIRT1-PGC1α regional brain distribution whole brain clearing and imaging

基于全矢谱时间固有尺度分解和独立分量分析盲源分离降噪的滚动轴承故障特征提取

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中国机械工程 2018年第8期29卷 943-948页

作者：刘嘉辉董辛旻李剑飞郑州大学机械工程学院郑州450001

结合盲源分离技术和全矢谱技术的各自优势,提出一种同源双通道信噪盲源分离法。首先采用时间固有尺度分解(ITD)和独立分量分析(ICA)相结合的分析法降噪,对同源双通道的轴承信号进行ITD分解,根据相关系数准则将分解得到的PRC分量进行重... 详细信息

结合盲源分离技术和全矢谱技术的各自优势,提出一种同源双通道信噪盲源分离法。首先采用时间固有尺度分解(ITD)和独立分量分析(ICA)相结合的分析法降噪,对同源双通道的轴承信号进行ITD分解,根据相关系数准则将分解得到的PRC分量进行重组作为ICA输入矩阵,再采用FastICA解混,实现故障信号与噪声信号的分离;其次采用全矢谱技术对信噪分离降噪后的双通道有效分量信号进行全矢信息融合,做全矢谱分析。滚动轴承故障实验对比分析表明了该方法的有效性。

关键词：时间固有尺度分解盲源分离独立分量分析全矢谱