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Human neuroblastoma cells transfected with two Chinese presenilin 1 mutations are sensitized to trophic factor withdrawal and protected by insulin-like growth factor-1

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Chinese Medical Journal 2008年第10期121卷 910-915页

作者： FANG Bo-yan jia jian-ping Department of Neurology First Affiliated Hospital of Liaoning Medical University Jinzhou Liaoning 121001 China Department of Neurology Xuanwu Hospital Capital Medical University Neurodegenerative Lab of Ministry of Education of the People's Republic of China Beijing 100053 China

Background Two novel presenilin 1 （PS1） mutations, V97L and A136G, were recently found to be involved in the early-onset of Alzheimer＇s disease in two Chinese families. This research aimed to verity their pathological effects. Methods The human neuroblastoma SH-SY5Y cells stably transfected with these two Chinese presenilin 1 mutations were established to explore whether they are sensitive to, or influenced by, serum deprivation and protected by insulin-like growth factor-1 （IGF-1）. Apoptosis rate, glucose uptake of the cells and the expression of glucose transport protein 1 （GLUT1） on cell membranes were examined. Results The V97L or A136G mutants significantly decreased the cells viability and increased the apoptosis rate when compare to PSlwt and mock transfected cells. IGF-1 was found to improve the viability of these two kinds of mutant cells significantly, and to show a protective effect for the mutants when they were treated with trophic deprivation. The glucose uptake of each transfected cell line increased to about 25% after IGF-1 treatment, GLUT1 expression on the cell membrane increased modestly by about 15%-20%. Conclusions Enhanced sensitivity to trophic withdrawal in the cells transfected with the two Chinese PS1 mutations may contribute to the neuron apoptosis, IGF-1 provided a protective effect to cells, possibly through an enhanced glucose transport and mitochondrial activities.

关键词： Alzheimer disease presenilin 1 mutation apoptosis insulin-like growth factor 1

Resistance of barley varieties to Heterodera avenae in the Qinghai–Tibet Plateau,China

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Journal of Integrative Agriculture 2022年第5期21卷 1401-1413页

作者： YAN jia-hui jia jian-ping jianG Li-ling PENG De-liang LIU Shi-ming HOU Sheng-ying YU Jing-wen LI Hui-xia HUANG Wen-kun Key Laboratory of Agricultural Integrated Pest Management Qinghai Academy of Agriculture and Forestry SciencesXining 810016P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China College of Plant Protection Gansu Agricultural UniversityBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China

The cereal cyst nematode,Heterodera avenae,is one of the most economically important pathogens impacting the worldwide production of cereals and is widely distributed in more than 16 regions in China.The present study used the numbers of nematodes inside the plant roots to evaluate the resistance/susceptibility of different subpopulations of barley Hordeum vulgare(QH2R,QH6R and TB2R)to H.avenae under field and pot conditions.Nematode development in two highly resistant varieties was also evaluated by in vivo experiment and microscopic observation.Analyses of 186selected varieties showed the numbers of susceptible varieties identified with the number of females/cysts per plant(NFP)method were significantly higher than those identified with the Pf/Pi ratio(PPR)method,which indicated that the NFP method rather than the PPR method is more reliable to evaluate the resistance of barley.The field and pot experiment results indicated that the QH2R subpopulation had lower females/cysts numbers than QH6R and TB2R subpopulations,and eight HR varieties(Sunong 7617,Sunong 7635,Dongyuan 87-14,Rudong 14-46,Rudong 87-57,Rudong 87-8-45,Rudong 88-14-2,and Rudong 88-67-1)were identified in QH2R,with the NFP numbers below 4.2.Further microscopic observation of nematode development suggested that H.avenae often penetrated less into highly resistant varieties(Sunong 7635 and Dongyuan 87-14)and more frequently failed to develop into females than the susceptible barleys.The promising resistant varieties identified in the present research might be helpful for breeders to develop CCN-resistant cultivars and control H.avenae populations effectively at low costs.

关键词： Heterodera avenae barley varieties resistance evaluation number of females/cysts per plant(NFP)

Recurrent Fisher-Bickerstaff syndrome： report of a Chinese case

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Chinese Medical Journal 2011年第17期124卷 2786-2788页

作者： DONG Hui-qing LIU Zheng TANG Yi LU Yan WANG Qi jia jian-ping Department of Neurology Xuanwu Hospital Capital Medical University Beijing 100053 China Key Neurodegenerative Lab of Ministry of Education of the People's Republic of China Beijing 100053 China

Fisher-Bickerstaff syndrome （FBS） was recently proposed to help to diagnose the conditions that overlap Fisher syndrome and Bickerstaff＇s brainstem encephalitis, as well as the unclassified conditions that had ophthalmoplegia and ataxia with clear consciousness, flexor plantar response and preserved tendon reflexes. Recurrences are exceptional with Guillain-Barre syndrome and its variants. Here we reported a patient with diagnosis of recurrent FBS. The patient presented with recurrent drowsiness, unsteady gait, diplopia and reduced deep tendon reflexes, which met the diagnostic criteria for FBS. The interval was eight months. He was treated with intravenous immunoglobulins during each episode and got good recovery. To our knowledge, this is a relatively early report about recurrent FBS case that had central and peripheral involvement during each episode in China.

关键词： Fisher syndrome Bickerstaff＇s brainstem encephalitis Guillain-Barr~ syndrome recurrent

Intracranial hypotension and co-existent normal-pressure glaucoma： the Beijing intracranial and intraocular pressure study

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Chinese Medical Journal 2013年第8期126卷 1588-1589页

作者： LI Zhen YANG Di-ya LU Yan LIU Da-chuan jia jian-ping Jonas Jost B WANG Ning-li Department of Ophthalmology Xuan Wu Hospital CapitalMedical University Beijing 100053 China Department of Neurology Xuan Wu Hospital CapitalMedical University Beijing 100053 China Beijing Tongren Eye Center Beijing Tongren Hospital CapitalMedical University Beijing Ophthalmology & Visual Sciences KeyLaboratory Beijing 100730 China Department of Ophthalmology Medical Faculty Mannheim of theRuprecht-Karls-University Heidelberg Mannheim Germany

n contrast to the so-called intraocular pressure, which is .the transcomeal pressure difference, it is thetrans-lamina cribrosa pressure difference, which is of importance for physiology and pathophysiology of the optic nerve head. The trans-lamina cribrosa pressure difference is the difference between the pressure in the intraocular compartment （i.e., the so-called intraocular pressure） minus the retrobulbar pressure, which is formed by the orbital cerebrospinal fluid （CSF）I pressure and the retrobulbar optic nerve tissue pressure. This anatomical fact has been the basis for the speculation that some patients with so-called normal intraocular pressure glaucoma have an abnormally low orbital CSF pressure. Previous clinical studies have supported this hypothesis.2＇3 The purpose of this study is to report on a patient with low CSF pressure and co-existent open-angle glaucoma with normal intraocular pressure.

关键词： intracranial pressure, glaucoma trans-lamina pressure difference

Transcriptomic analysis identifies the neuropeptide cortistatin (CORT) as an inhibitor of temozolomide (TMZ) resistance by suppressing the NF-kB-MGMT signaling axis in human glioma

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Genes & Diseases 2024年第3期11卷 385-399页

作者： Zongze He Bo Peng Qi Wang Jie Tian ping Liu Jie Feng Yiwei Liao Longyi Chen ping jia jian Tang Department of Neurosurgery Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Rehabilitation Medicine Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Neurosurgery Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Glioma is a common tumor originating in the brain that has a high mortality rate.Temozolomide(TMZ)is the first-line treatment for high-grade gliomas.However,a large pro-portion of gliomas are resistant to TMZ,posing a great challenge to their treatment.In the study,the specific functions and mechanism(s)by which cortistatin(CORT)regulates TMZ resis-tance and glioma progression were evaluated.The decreased expression of CoRT was detected in glioma tissues,and highly expressed CORT was associated with a better survival rate in pa-tients with glioma.CORT overexpression notably decreased the capacity of glioma cells to pro-liferate and migrate in vitro and to form tumors in vivo.CORT overexpression also markedly suppressed the viability and enhanced the apoptosis of TMZ-resistant U251 cells by regulating MGMT,p21,and Puma expression.Importantly,CORT overexpression reduced the resistance of gliomas to TMZ in vivo.CORT expression Was negatively correlated with MGMT expression in both glioma tissues and cells,and it was found that CORT inhibited NF-kB pathway activation in glioma cells,thereby inhibiting MGMT expression.In conclusion,CORT regulates glioma cell growth,migration,apoptosis,and TMZ resistance by weakening the activity of NF-kB/p65 and thereby regulating MGMT expression.The CORT/NF-kB/MGMT axis might be regarded as a molecular mechanism contributing to the resistance of glioma to TMZ.Our data also suggest that CORT regulates the viability and metastatic potential of glioma cells,independent of its effects on TMZ resistance,providing evidence of novel therapeutic targets for glioma that should be evaluated infurther studies.

关键词： Cortistatin Glioma MGMT NF-kB pathway Temozolomide

Cardioembolic occlusion of the internal carotid artery presented with infarction in the posterior cerebral artery territory

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Chinese Medical Journal 2010年第22期123卷 3375-3376页

作者： XUE Su-fang jia jian-ping Department of Neurology Xuanwu Hospital Capital Medical University Beijing 100053 China

Posterior circulation stroke may rarely be associated with occlusive disease in the anterior circulation, such as in the context of a direct （fetal） origin of the posterior cerebral artery （PCA） from the internal carotid artery （ICA）, or in the presence of a persistent trigeminal artery （PTA） or persistent hypoglossal artery （PHA）. Usually, they happened as infarction involving both ipsilateral carotid and posterior cerebral artery territories with carotid atherosclerosis etiology.3 Here, we reported a rare case with cadioembolic occlusion of the ICA presented with infarction mainly in the PCA territory.

关键词： carotid artery, internal cardioembolic occlusion posterior circulation stroke

Effect of screening colonoscopy frequency on colorectal cancer mortality in patients with a family history of colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 354-363页

作者： Li Zheng Bin Li Ling Lei Li-jia Wang Zhi-ping Zeng jian-Dong Yang Department of Gastroenterology The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina Department of General Surgery The First Hospital of NanchangNanchang 330008Jiangxi ProvinceChina

BACKGROUND Colorectal cancer is a common malignant tumor in China,and its incidence in the elderly is increasing annually.Inflammatory bowel disease is a group of chronic non-specific intestinal inflammatory diseases,including ulcerative colitis and Crohn’s disease.We included the clinicopathological and follow-up data of patients with colorectal cancer who underwent laparoscopic colectomy or open colectomy at our Gastrointestinal Department between January 2019 and December 2022.Surgical indicators,oncological indicators,and survival rates were compared between the groups.The results of 104 patients who met the above criteria were extracted from the database(laparoscopic colectomy group=63,open colectomy group=41),and there were no statistically significant differences in the baseline data or follow-up time between the two groups.RESULTS Intraoperative blood loss,time to first ambulation,and time to first fluid intake were significantly lower in the laparoscopic colectomy group than in the open colectomy group.The differences in overall mortality,tumor-related mortality,and recurrence rates between the two groups were not statistically significant,and survival analysis showed that the differences in the cumulative overall survival,tumor-related survival,and cumulative recurrence-free rates between the two groups were not statistically significant.CONCLUSION In elderly patients with colorectal cancer,laparoscopic colectomy has better short-term outcomes than open colectomy,and laparoscopic colectomy has superior long-term survival outcomes compared with open colectomy.

关键词： Colorectal cancer Laparoscopic surgery Open surgery Prognosis Laparoscopic colectomy Open colectomy

Anti-OX40 Antibody Combined with HBc VLPs Delays Tumor Growth in a Mouse Colon Cancer Model

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Biomedical and Environmental Sciences 2024年第2期37卷 187-195页

作者： LIU jia jia SU Qiu Dong YI Yao SHEN Li ping BI Sheng Li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective Combination immunotherapy strategies targeting OX40,a co-stimulatory molecule that can enhance antitumor immunity by modulating the proliferation,differentiation,and effector function of tumor-infiltrating T cells,have attracted much attention for their excellent therapeutic effects.In this study,we aimed to evaluate the antitumor efficacy of combined anti-OX40 and hepatitis B core viruslike particles(HBc VLPs)therapy using a mouse colon cancer model.Methods Humanized B-h OX40 mice were injected subcutaneously with MC38 colon tumor cells and treated with HBc VLPs+anti-h OX40 antibody.Tumor growth was monitored.Flow cytometric analysis was performed to evaluate the populations of T cell subsets in the tumors.Results The combination of anti-OX40 with HBc VLPs resulted in a significant delay in tumor growth,suggesting that a potent antitumor immunity was induced by the combination therapy.Further studies revealed that HBc VLPs+anti-OX40 treatment induced a significant increase in effector T cells(Teffs)and a significant decrease in regulatory T cells(Tregs)in the tumor microenvironment(TME),which accounted for the synergistic antitumor effect of anti-OX40 in combination with HBc VLPs.Conclusion Combination therapy of anti-h OX40 and HBc VLPs provides synergistic antitumor activity in colon cancer-bearing mice,which may represent a potential design strategy for cancer immunotherapy.

关键词： Anti-OX40 antibody Hepatitis B core virus-like particles Tumor Combination therapy

Clinicopathologic analysis of eight cases of pancreatic carcinoid tumors

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Chinese Medical Journal 2009年第13期122卷 1591-1594页

作者： HE Xiao-wen WU Xiao-jian HE Xiao-sheng ZOU Yi-feng KE jia WANG jian-ping LAN ping Department of Colorectal Surgery the Sixth Affiliated Hospital Sun Yat-Sen University Guangzhou Guangdong 510655 China

Carcinoids of the pancreas are exceedingly rare tumors that originate from the enterochromaffin cells of the gastroenteropancreatic neuroendocrine system. According to a recent report, pancreatic carcinoids are found in only 0.58% （79/13 715 cases） of the entire carcinoid group. To date, very limited information regarding the detection and diagnosis of this entity has been reported in the available literature. Although pancreatic carcinoid tumors grow slowly and show an indolent clinical behavior, they are potentially malignant. Due to the late onset of clinical symptoms and delayed diagnosis of the tumor, there was a high incidence of distant metastases that hampered long-term survival for many patients, leading to an unfavorable overall prognosis.

关键词： carcinoid tumor pancreas pathology metastasis