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Immunotoxicity of Acrylamide in Female BALB/c Mice

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Biomedical and Environmental Sciences 2014年第6期27卷 401-409页

作者： FANG Jin LIANG Chun Lai jia xu dong LI Ning Key Laboratory of Food Safety Risk Assessment Ministry of Health National Center for Food Safety Risk Assessment National Institute for Nutrition and Food Safety Chinese Center for Disease Control and Prevention

Objective To investigate the immunotoxicity of acrylamide （ACR） in female BALB/c mice.Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups （10 mice per group）： negative control, positive control （cyclophosphamide）, low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity. Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen. %T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of % natural killer（NK） cells and increase of %Th cells were observed in the ACR-H group. interleukin-6（IL-6）, Concanavalin A（ConA）-induced splenocyte proliferation and serum half hemolysis value （HCso） were also significantly suppressed in the ACR-H group. Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.

关键词： Acrylamide（ACR） Immunotoxicity Female BALB/c mice

A Subchronic Toxicity Study on Lactobacillus Fermentum GM 090 in Rat

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Biomedical and Environmental Sciences 2013年第8期26卷 680-683页

作者： jia xu dong ZHANG Xiao Peng LI Ning Key Laboratory of Food Safety Risk Assessment of Ministry of Health China National Center for Food Safety Risk Assessment

Lactobacillus fermentum （L. fermentum） is one of seven species in the genus Lactobacillus[1]. With a long history of safe use in fermented food production, Lactobacillus is considered as one of the most beneficial probiotics[23]. The most well-known potential health benefit is improving digestion and immune function[4.s]. Other beneficial functions of Lactobacillus strains include managing lactose intoleranceIs], lowering cholesterol and blood pressureIs], reducing inflammation[~], and prevention of cancerIs＇7]. L. fermentum is usually found during malt whisky fermentation]8]. L. fermentum CP34 was found to have the significant effect of decreasing the serum antigen-specific IgE levels compared to a control group[9].

关键词： A Subchronic Toxicity Study on Lactobacillus Fermentum GM 090 in Rat body GM

Immunotoxicological Evaluation of Wheat Genetically Modified with TaDREB4 Gene on BALB/c Mice

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Biomedical and Environmental Sciences 2013年第8期26卷 663-670页

作者： LIANG Chun Lai ZHANG Xiao Peng SONG Yan jia xu dong Key Laboratory of Food Safety Risk Assessment Ministry of Health (China National Center for Food Safety Risk Assessment)

Objective To evaluate the immunotoxicological effects of genetically modified wheat with TaDREB4 gene in female BALB/c mice. Methods Female mice weighing 18-22 g were divided into five groups （10 mice/group）, which were set as negative control group, common wheat group, parental wheat group, genetically modified wheat group and cyclophosphamide positive control group, respectively. Mice in negative control group and positive control group were fed with AIN93G diet, mice in common wheat group, non-genetically modified parental wheat group and genetically modified wheat group were fed with feedstuffs added corresponding wheat （the proportion is 76%} for 30 days, then body weight, absolute and relative weight of spleen and thymus, white blood cell count, histological examination of immune organ, peripheral blood lymphocytes phenotyping, serum cytokine, serum immunoglobulin, antibody plaque-forming cell, serum half hemolysis value, mitogen-induced splenocyte proliferation, delayed-type hypersensitivity reaction and phagocytic activities of phagocytes were detected. Results No immunotoxicological effects related to the consumption of the genetically modified wheat were observed in BALB/c mice when compared with parental wheat group, common wheat group and negative control group. Conclusion From the immunotoxicological point of view, results from this study demonstrate that genetically modified wheat with TaDREB4 gene is as safe as the parental wheat.

关键词： Genetically modified wheat Immunotoxicological effects BALB/c mice

Effects of Parental Dietary Exposure to GM Rice TT51 on the Male Reproductive System of Rat Offspring

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Biomedical and Environmental Sciences 2016年第4期29卷 267-274页

作者： WANG Er Hui YU Zhou jia xu dong ZHANG Wen Zhong xu Hai Bin Department of Nutrition and Food Hygiene College of Public Health Xinxiang Medical University Xinxiang 453003 Henan China Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine Xinxiang Medical University Xinxiang 453003 Henan China China National Center for Food Safety Risk Assessment Beijing 100021 China

Objective To evaluate the health effects of parental dietary exposure to GM rice TT52 on the male reproductive system of rat offspring. Methods Rice-based diets, containing 60% ordinary grocery rice, MingHui63, or TT51 by weight, were given to parental rats （15 males/30 females each group） for 70 days prior mating and throughout pregnancy and lactation. After weaning, eight male offspring rats were randomly selected at each group and fed with diets correspondent to their parents＇ for 70 days. The effects of exposure to TT52 on male reproductive system of offspring rats were assessed through sperm parameters, testicular function enzyme activities, serum hormones （FSH, LH, and testosterone levels）, testis histopathological examination, and the relative expression levels of selected genes along the hypothalamic-pituitary- testicular （HPT） axis. Results No significant differences were observed in body weight, food intake, organ/body weights, serum hormone, sperm parameters, testis function enzyme ACP, LDH, and SDH activities, testis histopathological changes, and relative mRNA expression levels of GnRH-R, FSH-R, LH-R, and AR along the HPT axis. Conclusion The results of this study demonstrate that parental dietary exposure to TT51 reveals no significant differences on the reproductive system of male offspring rats compared with MingHui63 and control.

关键词： Genetically modified rice Sperm parameter Hypothalamic-pituitary-testicular axis Reproductive toxicity

Combined Subchronic Toxicity of Bisphenol A and Dibutyl Phthalate on Male Rats

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Biomedical and Environmental Sciences 2013年第1期26卷 63-69页

作者： ZHANG Wen Zhong YONG Ling jia xu dong LI Ning FAN Yong Xiang China National Center for Food Safety Risk Assessment

Objective To evaluate the combined subchronic toxicity of bisphenol A（BPA） and dibutyl phthalate（DBP） in male Sprague Dawley（SD） rats.Methods Forty 4‐week‐old male rats weighing 115‐125 g were randomly divided into BPA‐treated,DBP‐treated group,BPA＋DBP‐treated and control groups and fed with a soy‐ and alfalfa‐free diet containing 285.4 ppm BPA,285.4 ppm DBP,285.4 ppm BPA plus 285.4 ppm DBP,and a control diet,respectively,for 90 consecutive days.At the end of the study,the animals were sacrificed by exsanguination via the carotid artery under diethyl etherane aesthesia and weighed.Organs,including liver,kidneys,spleen,thymus,heart,brain,and testis underwent pathological examination.The androgen receptor（AR）,gonadotropin‐releasing hormone receptor（GNRHR）,and progesterone hormone receptor（PR） genes from the hypothalamus were detected by real‐time PCR.The biomedical parameters were analyzed.Results No significant difference was found in food intake,body weight,tissue weight,organ/brain weight ratio,and biomedical parameters among the four groups（P〉0.05）.However,BPA and DBP up‐regulated AR,PR and GNRHR expression levels in rats and showed a synergistic or an additive effect in the BPA＋DBP group.Conclusion The combined subchronic toxicity of BPA and DBP is synergistic or additive in male SD rats.

关键词： Bisphenol A Dibutyl phthalate Combined subchronic toxicity Sprague dawley rat Endocrine disruption

Endocrine Disruption of Cadmium in Rats Using the OECD Enhanced TG 407 Test System

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Biomedical and Environmental Sciences 2014年第12期27卷 950-959页

作者： WANG Heng Juan LIU Zhao Ping jia xu dong CHEN Hao TAN Yan Jun Key Laboratory of China National Center for Food Safety Risk Assessment NHFPC

Objective To evaluate the endocrine disrupting effects of cadmium （Cd） using OECD enhanced TG407 test guideline. Methods Sprague-Dawley （SD） rats were randomly divided into six groups and accordingly administered with 0, 1, 2.5, 5, 10, 20 mg/kg.BW/day of Cd by gavage for 28 days. Body weight, food consumption, hematology, biochemistry, sex hormone levels, urinary β2-microglobulin, organ weights and histopathology and estrous cycle were detected. Results Cd could significantly decrease animals＇ body weight （P〈0.05）. Serum luteinizing hormone （LH） at 10-20 mg/kg.BW groups and testosterone （T） at 2.5 and 10 mg/kg.BW groups decreased significantly （P〈0.05）. However, no statistically significant change was found in urinary β2-microglobulin among Cd-treatment groups （P〉0.05）. Endpoints related to female reproduction including uterus weight and histopathological change at 10-20 mg/kg.BW groups showed significant increase （P〈0.05）. While among male rats in 2.5, 10, 20 mg/kg.BW groups, weight of prostate, thyroids, and seminal vesicle glands significantly decreased （P〈0.05）. Moreover, no histopathological change was observed in kidney. Conclusion Results suggested that Cd can cause endocrine disrupting effects in SD rats. Comparing with possible renal toxicity of Cd, its toxicity on endocrine system was more sensitive.

关键词： OECD Enhanced Test Guideline 407 Endocrine Disrupting Cadmium Renal toxicity

Neurobehavioral Assessment of Rats Exposed to Yttrium Nitrate during Development

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Biomedical and Environmental Sciences 2015年第4期28卷 281-290页

作者： LI Chen Xi MA Chuan FANG Hai Qin ZHI Yuan YU Zhou xu Hai Bin jia xu dong National Institute for Nutrition and Food Safety Chinese Center for Disease Control and Prevention Key Laboratory of Food Safety Risk Assessment of Ministry of Health China National Center for Food Safety Risk Assessment

Objective The aim of this study was to assess the effects of yttrium nitrate on neurobehaviora development in Sprague-Dawley rats. Methods Dams were orally exposed to 0, 5, 15, or 45 mg/kg daily of yttrium nitrate from gestation day （GD） 6 to postnatal day （PND） 21. Body weight and food consumption were monitored weekly. Neurobehavior was assessed by developmental landmarks and reflexes, motor activity, hot plate, Rota-rod and cognitive tests. Additionally, brain weights were measured on PND 21 and 70. Results No significant difference was noted among all groups for maternal body weight and food consumption. All yttrium-exposed offspring showed an increase in body weight on PND 21; however, no significant difference in body weight for exposed pups versus controls was observed 2 weeks or more after the yttrium solution was discontinued. The groups given 5 mg/kg daily decreased significantly in the duration of female forelime grip strength and ambulation on PND 13. There was no significant difference between yttrium-exposed offspring and controls with respect to other behavioral ontogeny parameters and postnatal behavioral test results. Conclusion Exposure of rats to yttrium nitrate in concentrations up to 45 mg/kg daily had no adverse effects on their neurobehavioral development.

关键词： Rare earth elements Yttrium Developmental neurotoxicity Neurobehavior

Lipid Accumulation and IL-6 Production in L02 Hepatocytes Induced by Sodium Oleate:Dose and Time Dependence

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Biomedical and Environmental Sciences 2021年第12期34卷 998-1004页

作者： CHEN Chen HUO Jun Sheng ZHUO Qin LI Yan MA Yan WANG Jing Bo jia xu dong Key Laboratory of Trace Element Nutrition of National Health Commission(NHC) National Institute for Nutrition and HealthChinese Center for Disease Control and PreventionBeijing 100050China Key Laboratory of Food Safety Risk Assessment of Ministry of Health China National Center for Food Safety Risk AssessmentBeijing 100021China

To explore interleukin-6(IL-6)production and characterize lipid accumulation in L02 hepatocytes induced by sodium oleate.L02 hepatocytes were incubated with 0,37.5,75,150,300,600,or 1,200μmol/L sodium oleate for 24 h,and the supernatant was collected to detect the concentration of IL-6.L02 hepatocytes were incubated with 300,150,75,or 0μmol/L sodium oleate for 0–24 h.The supernatant was collected for detection of IL-6 and free fatty acids.L02 hepatocytes treated with 300μmol/L sodium oleate for 0–24 h were stained with Oil Red O.With extended sodium oleate incubation time,IL-6 levels increased,and free fatty acids decreased.After 24 h incubation,IL-6 levels increased as sodium oleate increased from 37.5 to 300μmol/L(P<0.05 for 37.5μmol/L,P<0.01 for 75μmol/L and P<0.001 for concentrations 150μmol/L or higher).Lipid accumulation increased as the sodium oleate concentration and incubation time increased.Oil Red O staining intensified with incubation time extending beyond 2 h.IL-6 production and lipid accumulation in L02 hepatocytes are influenced by sodium oleate in a dose-and time-dependent manner.

关键词： L02 intensified Lipid

Subchronic Oral Toxicity Evaluation of Lanthanum： A 90-day, Repeated Dose Study in Rats

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Biomedical and Environmental Sciences 2018年第5期31卷 363-375页

作者： FANG Hai Qin YU Zhou ZHI Yuan FANG Jin LI Chen Xi WANG Yi Mei PENG Shuang Qing jia xu dong Key Laboratory of Food Safety Risk Assessment of Ministry of Health China National Center for Food Safety Risk AssessmentBeijing 100021China Evaluation and Research Centre for Toxicology Institute of Disease Control and PreventionAcademy of Military Medical SciencesBeijing 100071China

Objective The present study was undertaken to evaluate the subchronic toxicity of lanthanum and to determine the no observed adverse effect level（NOAEL）,which is a critical factor in the establishment of an acceptable dietary intake（ADI）.Methods In accordance with the Organization for Economic Co-operation and Development（OECD） testing guidelines,lanthanum nitrate was administered once daily by gavage to Sprague-Dawley（SD） rats at dose levels of 0,1.5,6.0,24.0,and 144.0 mg/kg body weight（BW） per day for 90 days,followed by a recovery period of 4 weeks in the 144.0 mg/kg BW per day and normal control groups.Outcome parameters were mortality,clinical symptoms,body and organ weights,serum chemistry,and food consumption,as well as ophthalmic,urinary,hematologic,and histopathologic indicators.The benchmark dose（BMD） approach was applied to estimate a point of departure for the hazard risk assessment of lanthanum.Results Significant decreases were found in the 144.0 mg/kg BW group in the growth index,including body weight,organ weights,and food consumption.This study suggests that the NOAEL of lanthanum nitrate is 24.0 mg/kg BW per day.Importantly,the 95% lower confidence value of the benchmark dose（BMDL） was estimated as 9.4 mg/kg BW per day in females and 19.3 mg/kg BW per day in males.Conclusion The present subchronic oral exposure toxicity study may provide scientific data for the risk assessment of lanthanum and other rare earth elements（REEs）.

关键词： Lanthanum Subchronic toxicity 90-day repeated oral dose test No observed adverse effectLevel Acceptable dietary intake Benchmark dose