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Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

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Journal of Integrative Agriculture 2020年第11期19卷 2746-2757页

作者： ji shun-xia SHEN xiao-na LIANG Lin WANG xiao-di LIU Wan-xue WAN Fang-hao LV Zhichuang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120P.R.China

Insects are poikilothermic animals,and temperature is one of the most important abiotic factors affecting their spread and *** example,differences in thermal tolerance may underlie the significant differences in geographical distributions between the native AsiaII3 and invasive MED(Mediterranean)cryptic Bemisia tabaci species in *** receptor potential(TRP)channels are key components of the insect temperature perception system and act as molecular thermometers since they can be activated by specific changes in *** this study,we cloned and characterized the AsiaII3 BtTRP gene and revealed its functions in the response to thermal *** full-length cDNA of BtTRP was 3821 bp,with a 3501-bp open reading frame encoding a 132.05-kDa *** the deduced amino acid sequences of AsiaII3 BtTRP and MED TRP revealed five amino acid *** situ hybridization indicated that BtTRP might be widely expressed throughout the AsiaII3 adult *** mRNA expression reached the highest levels after exposure to mild thermal stimuli(12 and 35°C),showing that BtTRP expression can be induced by temperature ***,the thermal tolerance of AsiaII3 after BtTRP dsRNA feeding was significantly lower than that of the *** together,the present study highlights the importance of TRP channels for *** thermal resistance,and allows us to infer that the differences in amino acids between AsiaII3 and MED might cause the differences in thermal tolerance of these two cryptic *** study provides a new direction for investigating geographic distribution differences between invasive and native insects.

关键词： transient receptor potential thermosensation thermal stress Bemisia tabaci AsiaII3

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Role of nutrition in diabetes mellitus and infections

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World Journal of Clinical Cases 2025年第3期13卷 1-8页

作者： Xue-Lu Yu Li-Yun Zhou xiao Huang Xin-Yue Li Ming-Ke Wang ji-shun Yang Naval Medical Center of PLA Naval Medical UniversityShanghai 200052China

In this editorial,we have commented on the article that has been published in the recent issue of World Journal of Clinical *** authors have described a case of unilateral thyroid cyst and have opined that the acute onset of infection may be linked to diabetes mellitus(DM).We have focused on the role of nutrition in the association between DM and *** with DM are at a high risk of infection,which could also be attributed to nutrition-related *** interventions for patients with diabetes are mainly based on a low-calorie diet,which can be achieved by adhering to a low-carbohydrate ***,dietary fiber supplementation is recommended to maintain the diversity of the gut ***,high-quality protein can prevent the increased risk of infection due to *** of vitamins C,vitamins A,vitamins D,and folic acid improves blood sugar control and facilitates immune *** deficiencies augment the risk of infection,but the relationship with diabetes is mostly U-shaped and a good intake should be maintained.

关键词： Diabetes mellitus Infection Macronutrients Vitamins Minerals

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人von Willebrand因子A1区基因的克隆和表达

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中国实验血液学杂志 2002年第6期10卷 540-543页

作者：祝怀平王迎春季顺东白霞阮长耿苏州大学附属第一医院江苏省血液研究所苏州215007

为了深入研究血栓形成的机制以及开发新的抗血栓药物 ,将人vonWillebrand(vWF)因子A1区cDNA在大肠杆菌中表达 ,以获得高效表达的具有生物学活性的重组A1区蛋白 ,应用PCR方法从含有人全长vWFcDNA质粒中扩增A1区基因片段 ,并将其克隆至pUC... 详细信息

为了深入研究血栓形成的机制以及开发新的抗血栓药物 ,将人vonWillebrand(vWF)因子A1区cDNA在大肠杆菌中表达 ,以获得高效表达的具有生物学活性的重组A1区蛋白 ,应用PCR方法从含有人全长vWFcDNA质粒中扩增A1区基因片段 ,并将其克隆至pUCm T载体中 ,经DNA序列分析后 ,将其重组于有 6×HisTag的融合蛋白表达载体pQE 31中 ,并在大肠杆菌M1 5中诱导表达。采用Ni NTA柱进行纯化 ,用Western印迹鉴定。结果表明 ,PCR扩增得到 854bp的A1区基因片段 ,序列测定结果与文献报道一致。IPTG诱导 5小时后表达的vWF因子A1蛋白占菌体总蛋白的 30 %左右 ,经Ni NTA纯化后其纯度在 95 %以上。Western印迹检测显示A1蛋白具有很好的抗原性和特异性。结论 :成功地在大肠杆菌中高效表达了人vWFA1区蛋白。

关键词：基因克隆基因表达序列分析血友病 A1区 vonWillebrand因子

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抗血管性血友病因子A1区单链抗体噬菌体展示文库的构建和筛选

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上海免疫学杂志 2003年第5期23卷 331-335页

作者：祝怀平王迎春江淼季顺东白霞阮长耿苏州大学附属第一医院江苏省血液研究所苏州215006

为构建抗人vWF-A1的全套单链抗体噬菌体表面展示文库,从中筛选与vWF-Al高亲合力的单链抗体,为其临床应用奠定基础。从vWF-A1免疫小鼠的脾淋巴细胞中提取总RNA,纯化mRNA并反转录成cDNA后,用抗体可变区混合引物扩增全套轻、重链可变区基因... 详细信息

为构建抗人vWF-A1的全套单链抗体噬菌体表面展示文库,从中筛选与vWF-Al高亲合力的单链抗体,为其临床应用奠定基础。从vWF-A1免疫小鼠的脾淋巴细胞中提取总RNA,纯化mRNA并反转录成cDNA后,用抗体可变区混合引物扩增全套轻、重链可变区基因,经重叠延伸反应,在体外装配成单链抗体(scFv),将其克隆至噬菌体载体pHENl中,构建单链抗体噬菌体抗体库。对抗体库进行亲合筛选后,ELISA法鉴定抗vWF-A1单链抗体。结果经过5轮“吸附-洗脱-扩增”的富集过程,phage-ELISA筛选到与vwF-A1有高亲和力的克隆。序列测定符合抗体可变区结构特点。成功构建了全套抗vWF-A1单链抗体噬菌体展示文库,并筛选出具有结合vWF-A1功能的单链抗体。

关键词： vWF 单链抗体噬菌体抗体库

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Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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Traditional Medicine Research 2025年第2期10卷 16-29页

作者： xia-jie Quan Hao Liang Yong-Hong Tang Li jiang Xiong-Ying ji Feng-Ying Zhang Ping Zhang Bo Ouyang Department of Information The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Neurology The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Gastroscopy The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Traditional Chinese Medicine The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China

Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of ***,the specific mechanism of action of SXLJF remains ***:The mouse model of insomnia was established by intraperitoneal injection of ***-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive *** open field test and pentobarbital-induced sleeping test were ***-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating ***,key targets were validated using ***:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep *** and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of ***,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,***-qPCR results supported the role of TH in the mechanism of SXLJF in treating ***:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关键词： Shangxia Liangji formula insomnia metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism

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Vibration of Timoshenko beam on hysteretically damped elastic foundation subjected to moving load

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Science China(Physics,Mechanics & Astronomy) 2015年第8期58卷 58-66页

作者： LUO WeiLi xia Yong WENG shun Department of Civil and Environmental Engineering The Hong Kong Polytechnic University School of Civil Engineering & Mechanics Huazhong University of Science and Technology

The vibration of beams on foundations under moving loads has many applications in several fields, such as pavements in highways or rails in railways. However, most of the current studies only consider the energy dissipation mechanism of the foundation through viscous behavior; this assumption is unrealistic for soils. The shear rigidity and radius of gyration of the beam are also usually excluded. Therefore, this study investigates the vibration of an infinite Timoshenko beam resting on a hysteretically damped elastic foundation under a moving load with constant or harmonic amplitude. The governing differential equations of motion are formulated on the basis of the Hamilton principle and Timoshenko beam theory, and are then transformed into two algebraic equations through a double Fourier transform with respect to moving space and time. Beam deflection is obtained by inverse fast Fourier transform. The solution is verified through comparison with the closed-form solution of an Euler-Bernoulli beam on a Winkler foundation. Numerical examples are used to investigate:(a) the effect of the spatial distribution of the load, and(b) the effects of the beam properties on the deflected shape, maximum displacement, critical frequency, and critical velocity. These findings can serve as references for the performance and safety assessment of railway and highway structures.

关键词： vibration beam-foundation system moving load

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Diffusion tensor interpolation profile control using non-uniform motion on a Riemannian geodesic

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Journal of Zhejiang University-Science C(Computers and Electronics) 2012年第2期13卷 90-98页

作者： Chang-II SON shun-ren xia MOE Key Laboratory of Biomedical Engineering Zhejiang UniversityHangzhou 310027China Department of Electronics Kim Chaek University of TechnologyPyongyang 104919DPR of Korea

Tensor interpolation is a key step in the processing algorithms of diffusion tensor imaging (DTI), such as registration and tractography. The diffusion tensor (DT) in biological tissues is assumed to be positive definite. However, the tensor interpolations in most clinical applications have used a Euclidian scheme that does not take this assumption into account. Several Rie-mannian schemes were developed to overcome this limitation. Although each of the Riemannian schemes uses different metrics, they all result in a ‘fixed’ interpolation profile that cannot adapt to a variety of diffusion patterns in biological tissues. In this paper, we propose a DT interpolation scheme to control the interpolation profile, and explore its feasibility in clinical applications. The profile controllability comes from the non-uniform motion of interpolation on the Riemannian geodesic. The interpolation experiment with medical DTI data shows that the profile control improves the interpolation quality by assessing the reconstruction errors with the determinant error, Euclidean norm, and Riemannian norm.

关键词： Diffusion tensor (DT) DT imaging (DTI) DT interpolation Interpolation profile control Riemannian geodesic

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Adsorption properties of Ni cluster modified TiO_(2)(101)towards SF_(6)decomposition gases

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High Voltage 2023年第1期8卷 158-170页

作者： Yingang Gui shun Yang Chang ji xianping Chen College of Engineering and Technology Southwest UniversityChongqingChina State Grid Chongqing Beibei Power Supply Company ChongqingChina College of Optoelectronic Engineering and Key Laboratory of Optoelectronic Technology&Systems Education Ministry of China Chongqing UniversityChongqingChina

Online detection of insulation defects the decomposition products of SF_(6)plays a key role in ensuring the safe operation of the gas-insulated switchgear(GIS)*** pristine TiO_(2)(101)modified by Ni cluster(1-3 Ni atoms)is used as a new gas-sensing material to study its gas-sensing characteristics to the characteristic decomposition products of SF_(6):SO2,SOF2,and *** density functional theory(DFT)calculation,we found that the modification of Ni clusters significantly improved the conductivity of pristine TiO_(2)(101)and the gas sensitivity of SF_(6)decomposition *** importantly,the change of conductivity after gas adsorption lays a theoretical foundation for identifying the type and concentration of SF_(6)characteristic decomposition products and further judging the type of defects in GIS.

关键词： cluster modified conductivity

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Symmetry Reduction and Cauchy Problems for a Class of Fourth-Order Evolution Equations

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Communications in Theoretical Physics 2008年第7期50卷 31-38页

作者： LI ji-Na ZHANG shun-Li Center for Nonlinear Studies Department of Mathematics Northwest University Xi＇an 710069 China Center of Nonlinear Science Ningbo University Ningbo 315211 China

We exploit higher-order conditional symmetry to reduce initial-value problems for evolution equations toCauchy problems for systems of ordinary differential equations (ODEs).We classify a class of fourth-order evolutionequations which admit certain higher-order generalized conditional symmetries (GCSs) and give some examples to showthe main reduction *** reductions cannot be derived within the framework of the standard Lie approach,which hints that the technique presented here is something essential for the dimensional reduction of evolu tion equations.

关键词： fourth-order evolution equation generalized conditional symmetry Cauchy problem

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A Pair Correlation Function Characterizing the Anisotropy of Force Networks

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Chinese Physics Letters 2011年第6期28卷 176-178页

作者： SUN Qi-Cheng ji shun-Ying State Key Laboratory of Hydroscience and Engineering Tsinghua UniversityBeijing 100084 State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian 116023

Force networks may underlie the constitutive relations among granular solids and granular flows and inter-state ***,it is difficult to effectively describe the anisotropy of force *** propose a new pair correlation function g(r,θ)to describe the characteristic lengths and orientations of force chains that are composed of particles with contact forces greater than the threshold values.A formulation g(r,θ)≈a(r)+b(r)cos2(θ−π/2)is used to fit the g(r,θ)*** characteristic lengths and orientations of force networks are then elucidated.

关键词： networks characteristic anisotropy

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